• Bulletin of the Korean Chemical Society
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• 제27권8호
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• pp.1119-1120
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• 2006

• Bulletin of the Korean Chemical Society
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• 제30권4호
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• pp.948-950
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• 2009

• Journal of Yeungnam Medical Science
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• 제15권2호
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• pp.286-296
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• 1998

비중격 만곡증 환자의 비내수술시 채취한 정상 하비갑개 점막조직으로 부터 상피세포만을 분리세포의 단층배양법(monolayer culture of dissociated cells)으로 배양하여 비점막 상피세포 배양법을 정립하고 또한 배양된 세포가 상피세포임을 동정하기 위하여 간접 면역형광항체법으로 상피세포 특유의 cytokeratin을 확인하였고 투과전자현미경으로 상피세포만이 가지고 있는 교소체(desmosome) 및 장세사(tonofilament) 등을 확인하였으며 6회까지 계대배양을 할 수 있었다. 향후 본 연구에서 배양된 비점막 상피세포를 이용하여 알레르기성 비염 등과 같은 비점막 염증성 질환의 병태생리에서 상피세포의 역할에 대한 연구와 호산구등 염증 세포의 침윤과 상피세포의 손상에 접착분자 및 cytokine의 상호작용에 관한 연구가 계속될 수 있을 것으로 기대한다.

• 대한화학회지
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• 제37권6호
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• pp.570-576
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• 1993

물 표면 위의 스테아르산 단분자막을 은을 입힌 유리판에 이전시켜 1층, 3층, 9층 및 21층의 Langmuir-Blodgett(LB) 분자막을 제조하였다. 표면압은 30 mN/m 이었으며 FTIR-RA 분광법을 이용하여 31~72$^{\circ}C$에서 적외선 흡수 스펙트럼을 얻었다. $31^{\circ}C$에서 탄화수소 사슬이 보다 더 수직적인 배향을 나타내고 있었으며 1층의 경우에는 C = O의 피크가 관찰되지 않았다. 이는 은표면에서 이미지 이중극 효과나 금속염을 형성하기 때문으로 본다. 1층과 3층의 경우에는 스테아르산의 트란스 이성질체가 많고 21층의 경우에는 시스형이 많은 것으로 나타났다. 온도 변화에 대한 FTIR-RAS 측정에서 분자층의 수를 증가시키면 사슬의 녹는점이 높아졌으나 1층의 경우는 예외를 나타내는데 이는 1층의 경우 은기판과의 강한 상호작용에 의한 때문이다.

• 접착 및 계면
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• 제20권1호
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• pp.1-8
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• 2019

균일한 단일층(monolayer)의 형성은 박막 두께에서의 특성을 유지하면서 차광, 반사방지 등의 물리적, 화학적 기능을 강화할 수 있는 중요한 수단이다. 이전 연구에서도 여러 코팅 방법으로 단일층을 구현하였으나, 공정이 복잡하고 대면적화 하는데 어려움이 있었다. 이에 본 연구에서는 소량의 용액으로 대면적 코팅이 가능한 로드 (rod) 코팅법을 사용하여, $20cm{\times}20cm$ PET 필름 기판 위에 마이크로미터 크기의 PMMA 비드를 가장 치밀한 형태인 HCP(hexagonal closed packing)가 되도록 코팅을 진행하였다. 끓는점과 증기압이 다른 용매의 사용과 계면활성제의 적용, 플라즈마 처리를 통한 기판 에너지의 변화를 통해 형성되는 단일층의 수준을 관찰하였다. 본 연구를 통해 비드의 메니스커스, 용매와 비드의 인-척력, 표면에너지를 포함한 코팅 조건을 최적화함으로써, 최종적으로 단위면적당 비드가 차지하는 정도인 입자의 커버리지를 초기 대비 약 20% 정도 향상시켰고, 단일층에 영향을 주는 인자들을 확인하였다. 본 결과는 기존에 연구되었던 코팅 방법에 비해 간단하고 빠르게 대면적의 단일층(monolayer)을 형성할 수 있기 때문에, 광학필름과 센서 등 첨단 분야로의 잠재적 응용 가능성이 높다.

• Bulletin of the Korean Chemical Society
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• 제31권5호
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• pp.1262-1266
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• 2010

In this study, we investigated the use of liquid crystals to selectively detect the activity of enzymes at interfaces decorated with oligopeptide-based membranes. We prepared a mixed monolayer of tetra(ethylene glycol)-terminated lipids and carboxylic acid-terminated lipids at the aqueous-liquid crystal (LC) interface. The 17 amino-acid oligopeptide SNFKTIYDEANQFATYK was then immobilized onto this mixed monolayer through N-hydroxysuccinimide-activation of the carboxylic acid groups. We examined the orientational behavior of nematic 4-cyano-4'-pentylbiphenyl (5CB) after conjugation of the 17 amino-acid oligopeptide with the mixed monolayer assembled at the interface. Immobilization of the oligopeptide caused orientational transitions in 5CB, with a change from homeotropic (perpendicular) to tilted alignment, which was primarily due to the reorganization of the monolayer. The orientation of the 5CB molecules returned to its homeotropic state after contacting the interface containing ${\alpha}$-chymotrypsin, which can cleave the immobilized oligopeptide. Control experiments confirmed that the enzymatic activity of ${\alpha}$-chymotrypsin triggered the ordering transitions in the LC. These results suggest that the LC can provide a facile method for selective detection of enzymatic activity.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 춘계학술대회 논문집 센서 박막재료 반도체재료 기술교육
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• pp.90-93
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• 2002

In the surface pressure-area isotherms of mixed monolayers, mixtures containing as much as 30 mol% of AA form stable condensed monolayer while the monolayer without AA is in the expanded state because PVK take on 3D collapsed. All of the mixed monolayers with 0, 10, 20 and 30 mol% of AA could be readily transferred onto ITO substrate at 16, 17, 24 and 26 mN/m, respectively. The monolayer containing 30 mol% of AA, however, showed a roughness value of 28A and became homogeneous decreasing with the phase separation. We fabricated organic EL device of ITO/CuPc/MEL/BBOT/iLiF/Al using mixed monolayer of 13, 19 and 25 layer deposited by LB method as a emitting layer. In the voltage-current characteristics of EL device, current density was much smaller than that of the spin-coated devices. It may due to the large contact resistance existed at the interface of LB layer/organic layer inhibit carrier injection to the emitting layer. EL spectra of device showed peaks at 450. 470, 505, 555 and 650 nm and the white light emission indicate the CIE coordinate x=0.306, y=0.353.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1991년도 추계학술대회 논문집 학회본부
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• pp.333-335
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• 1991

${\prod}-A$ characteristics and UV-visible spectrum of N-docosylquinolium-TCNQ monolayers were investigated. ${\prod}-A$ characteristics of N-docosylquinolium-TCNQ monolayer were measured following variation. i > ${\prod}-A$ characteristics with a variation of spreading amount. ii > ${\prod}-A$ characteristics with a variation of subphase temperature. An optium surface pressure for a LB Film deposition was found to be a 30, 45 mN/m in pure water subphase(pH 5.4, $25^{\circ}C$). UV-visible spectrum Analysis of these LB film were investigated. As a result, we think two kinds of surface pressure had no effect the monolayer aggregation but monolayer density.

• The Korean Journal of Physiology
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• 제10권1호
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• pp.7-14
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• 1976

The Present study was conducted to investigate the effects of Ginseng extract on the tension-area curve for stearic acid monolayer. At the same time, the effects of Ginseng extract on osmotic and mechanical fragility of human red cells and histamine release from rabbit leukocytes were studied, The results are summarized as follows. 1. The Ginseng alcohol extract was found to expand liquid expanded phase of stearic acid monolayer, thus it is speculated that this agent may be acting as a surface active substance. 2. Osmotic hemolysis was inhibited by the Ginseng alcohol extract and the same effect was also observed in the presence of Ginseng saponin. However, the Ginseng alcohol extract was found to decrease hematocrit ratio of the RBC suspension, therefore, the inhibition of the osmotic hemolysis by this agent may be secondary effect to the reduced cell volume. 3. The mechanical hemolysis was also inhibited by the Ginseng alcohol extract but the inhibition was independent of changes in hematocrit ratio. 4. Histamine release from rabbit leukocytes was significantly increased in vitro in the presence of the Ginseng alcohol extract.(p<0.05)

• 전기학회논문지
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• 제56권1호
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• pp.117-122
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• 2007

LB method is one of the most interesting technique to arrange certain molecular groups at precise position relative to others. Also, the LB deposition technique can fabricate extremely thin organic films with a high degree of control over their thickness and molecular architecture. In this way, new thin film materials can be built up at the molecular level, and the relationship between these artificial structures and the properties of materials can be explored. In this paper, evaluation of physical properties was made for dielectric relaxation phenomena by the detection of the surface pressures and displacements current on the monolayer films of phospolipid monomolecular DLPC. Lipid thin films were manufacture by detecting deposition for the accumulation and the current was measured after the electric bias was applied across the manufactured MIM device. It is found that the phospolipid monolayer of dielectric relaxation takes a little time and depend on the molecular area. When electric bias is applied across the manufactured MIM device by the deposition condition of phospolipid mono-layer, it wasn't breakdown when the higher electric field to impress by increase of deposition layers.