• Journal of Life Science
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• v.21 no.6
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• pp.819-828
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• 2011

To inspect norovirus contamination of groundwater in south eastern areas of Korea, a systematic survey of groundwater in Busan, Ulsan, and Gyeongsangnam-do was performed for two years from 2009 to 2010. For this purpose, we first optimized the nested reverse transcription-PCR condition by designing two sets of primers for the detection of norovirus genogroups, GI and GII. Of 145 samples, 21 (25.9%) and 15 (23.4%) were norovirus positive in the dry season (April to June) and wet season (July to August), respectively. The detection frequencies of norovirus in Busan, Ulsan, and Gyeongsangnam-do were 15%, 7%, and 32%, respectively, reflecting a geographical difference in their distribution. The GI and GII isolates were 5 and 31, respectively, indicating the prevalence of GII in the tested areas. According to phylogenetic analysis of their nucleotide sequences, all of the GI isolates were identified to genotype GI.5 whilst the GII isolates were divided into two genotypes, GII.3 and GII.4. Neither physical-chemical parameters such as pH, temperature, oxidation-reduction potential, and dissolved oxygen, nor microbial indicators of water quality such as total bacteria, total coliforms, and Escherichia coli were statistically correlated with contamination of norovirus in the groundwater. Interestingly, however, the presence of norovirus was closely correlated with low turbidity (<0.50 NTU). The present study suggests that periodical monitoring of norovirus in groundwater is necessary to prevent epidemic waterborne diseases and to secure better sanitary conditions for public health.

• Journal of Korean Society of Transportation
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• v.32 no.5
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• pp.431-440
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• 2014

According to the State Safety Program (SSP) of the International Civil Aviation Organization (ICAO), it is essential to establish and manage Safety Performance Indicators (SPIs) which are used for monitoring the safety performance to achieve the national aviation safety goal. There have been enormous efforts to develop the framework of SPIs by considering the current status for each country. In case of Republic of Korea, however, there has been limited research and policy projects related to the framework for SPIs. Furthermore, Korea has used identical SPIs since the SSP was legislated in 2008. With this background, this research is, as part of strategy for the state level of SPI development, the study cases of advanced aviation countries for SPI development plans, such as the European Aviation Safety Agency (EASA) of the EU, the Civil Aviation Authority (CAA) of the UK, the Finish Transport Safety Agency (FTSA) of the Finland and the Federal Aviation Administration (FAA) of the U.S. The comparison between the foreign and domestic policies for SPI development strategies are provided in the conclusion of this study. The results and analyses of the case studies performed in this research will be helpful to provide some valuable development strategies for further SPI research in Korea.

• Analytical Science and Technology
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• v.12 no.3
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• pp.190-195
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• 1999

The metabolism of testosterone ($17{\beta}$-hydroxy-androst-4-en-3-one) was confirmed in horse after a single intramuscular administration of testosterone cypionate (750 mg). Solvent extracts of urine obtained with enzymatic hydrolysis and methanolysis were analyzed by GC/MS after oxime t-butyldimethylsilyl (oxime-TBDMS) derivatization. The structures of four urinary metabolite after testosterone administration in horse were determined based on EI mass spectra and $5{\alpha}$-androstane-$3{\beta}$, $17{\alpha}$-diol and $5{\alpha}$-androstane-$3{\beta}$-ol-17-one as major was confirmed with authentic standard. Also the concentrations of $5{\alpha}$-androstane-$3{\beta}$, $17{\alpha}$-diol, $5{\alpha}$-androstane-$3{\beta}$, $17{\beta}$-diol, dehydroepiandrosterone (DHEA), $5{\alpha}$-androstane-$3{\beta}$-ol-17-one and testosterone were determined in the urine of normal subjects and the urine after administration. The recovery and detection limit in the most drugs were 86.3~94.7% and 1~3 ppb, respectively. Correlation coefficients for calibration were in the range of 0.984~0.999. Excretion profile of testosterone presents the rapid and large increasement up to maximum values at days 5 after administration and the slow regression. The relative ratios of testosterone, its metabolites over DHEA were determined for indication of testosterone administration in horse doping.

• Korean Journal of Environmental Agriculture
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• v.36 no.4
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• pp.288-292
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• 2017

BACKGROUND: Silicon tetrachloride reacts with moisture in the atmosphere to generate hydrogen chloride, which affects the environment. Since silicon tetrachloride and its by-products are dispersed in the atmosphere in a short time after the silicon tetrachloride release into the atmosphere, it is difficult to directly assess the extent of environmental impact. In the present study, the exposure test of silicon tetrachloride or hydrogen chloride was examined in order to establish the criterion of the range affected by the silicon tetrachloride release, and the actual crops in the area exposed to silicon tetrachloride leakage were analyzed. METHODS AND RESULTS: For the experiment of exposure to silicon tetrachloride or hydrogen chloride, the leaves of red-pepper and corn were used in glass sealed containers. In the actual accident area, 59 samples from 10 different kinds of crops were collected. The pretreatment of the sample was performed by freezing and grinding, and then extracted using distilled water. The pH and concentration of chloride ($Cl^-$) ion of the extracted solution were measured using pH meter and ion chromatograph, respectively. CONCLUSION: Exposure to silicon tetrachloride caused visible damage, increasing the concentration of chloride ion, and decreasing the pH as well as hydrochloric acid. In the actual crops of the affected area, the tendency was the same as the result of the laboratory test, and the range of influence could be estimated through the concentration of $Cl^-$ ion over 2,000 mg/kg, and the correlation evaluation between the concentration of $Cl^-$ and pH. Therefore, the concentration of $Cl^-$ ion and the correlation between $Cl^-$ and pH would be considered as the factors to estimate the influence range of silicon tetrachloride release.

• Korean Journal of Ecology and Environment
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• v.46 no.1
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• pp.103-115
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• 2013

The purpose of this study was to analyze fish fauna and species compositions along with trophic guilds, tolerance indicators, and fish community conditions before weir construction (BWC) and after weir construction (AWC) in the Yeongsan River watershed. Total 45 and 44 fish species were sampled in BWC and AWC, respectively without any distinct differences through weir constructions. Fish fauna analysis revealed that the dominant species was the same, Zacco platypus with 24.3% and 20.8% in BWC and AWC, respectively. However, the subdominant species were Carassius auratus with 8.9% in BWC but Opsarichthys uncirostris amurensis with 20.3%, almost identical that of the dominant species AWC. This phenomenon showed the distinct modification of species composition in the watershed. We sampled the $1^{st}$ class endangered species, Liobagrus obesus in tributary stream as previously reported. Also Culter brevicauda was sampled in the mainstream of Yeongsan River watershed and this was the first sampled record in this watershed. One of the most important features were an increase of exotic species, such as Micropterus salmoides and Lepomis macrochirus, with 3.2% BWC vs. 10.2% AWC as well as the increase in tolerant species with 49.2% BWC vs. 73.7% AWC, indicating ecological degradation through weir construction. Overall, our results indicated that fish fauna and composition analyses showed distinct ecological degradations related to increases of exotic and tolerant species AWC. Further long-term studies of fish monitoring should be conducted in the future to configure existent status of river conditions and to provide key information in order to conserve the healthy ecosystem.

• Ecology and Resilient Infrastructure
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• v.6 no.4
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• pp.227-235
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• 2019

This study monitored Daecheong 1-bo, Daecheong-stream, which carried out the project in 2014, from 2015 to 2016. The technology applied to the stream was evaluated using Periphytic Algae to check contamination indicators and ecological health of the area with an integral river-bed protection using non-toxic materials. The water quality of the monitoring section was confirmed to be above the river environment standard (II), and it was confirmed that the Saproxenic taxa of the river bed protection were higher than the upper and downstream sections. The TDI, which is an index of attachment algae, was shown in the average 51.03 and 52.15 for the pilot project sections in 2015 and 2016, confirming that the index is of the "normal" grade. This is the other sections in the upstream and downstream sections showed higher than "bad", which is thought to have a positive effect on the habitat of the river ecosystem components, especially the microbial population in river bed protection.

• Journal of the Korean Electrochemical Society
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• v.17 no.3
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• pp.172-178
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• 2014

In this work, we describe an electrochemical immunosensor for simple, fast and quantitative detection of a urinary hippuric acid (HA). Urinary HA, of molecular weight 180 DA, is one of the major metabolites and biological indicators in toluene-exposed humans. Simple and ubiquitous monitoring of exposure to toluene is very important in occupational health care. We propose the electrochemical immunoassay based on the dopamine-antigen conjugate for detecting hippuric acid. Our electrochemical immunoassay system employs a conjugate of dopamine (DA) as an electrochemical active molecule and hippuric acid (HA) as an antigen. As an electrochemical aspect, dopamine (DA) containing two hydroxyl group can show excellent redox signal. Also, dopamine-tethered hippuric acid (DA-HA) shows the reversible redox signal in the immunoassay. The competition between HA and DA-HA generated electric signals proportional to HA concentration. The electrochemical immunoassay was performed with DA-HA on the screen printed carbon electrodes (SPCEs), and then applies the mixture antigen (HA) and HA-antibody. The electrical signals were proportional to HA in the range of 0.010~2.500 mg/mL which is enough range to be used for the point-of-care.

• Journal of Korea Water Resources Association
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• v.53 no.spc1
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• pp.813-826
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• 2020

This study analyzes the effects of the Smart Water City (SWC) project that was introduced from 2014 to 2016 in Paju City, Gyeonggi Province, Korea, focusing on the achievement of the business goals. The SWC is referred to as a city that embraces a healthy water supply system based on Smart Water Management (SWM) that promotes the efficiency of water management by combining Information and Communication Technologies (ICTs) with water and sewerage facilities. In order to evaluate the effectiveness of the SWC project, this study deploys evaluation criteria corresponding to the project objectives, and analyzes the outputs before and after the project. The results show that the SWC has contributed to enhancing water supply services and the reliability and drinking rate of tap water. Specific improvement areas include the rise of average water flow rate and water leakage reduction, the diffusion of water quality monitoring system, and the reduction of floating particle concentration and turbidity in drainage pipes was achieved. These were possible because of specific implementation plans for clear goal setting and achievement and active services for citizens. The data related to water quantity and quality showed improved performance compared to before the introduction of SWMS, which is a positive effect. However, a quantitative analysis of the outputs has limitations in identifying other external factors that have led to the changes. In the future, guidelines for spreading SWC and more comprehensive and specific evaluation indicators for SWC should be prepared, and SWMS should be developed in consideration of the needs of users.

• Journal of Korean Society of Forest Science
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• v.109 no.3
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• pp.350-360
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• 2020

The purpose of this study was to identify and prioritize the key indicators and drivers of forest thinning. The research for this study was designed in two-phases: 1) sequential, exploratory, mixed methods research that was initiated with a qualitative phase (Delphi technique), and 2) the quantitative phase (Analytic Hierarchy Process technique). Results indicated that management and planning were the most important factors in the first level of criteria among the "management and planning," "directing and monitoring," "supervision," and "quality of thinning work." On the sub-criteria level, "the quality of forest management planning" was indicated as the most important factor among the ten sub-criteria. Our results have shown that the developed forest thinning evaluation factors were a well-represented characteristic for a variety of forest thinning work in Korea.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.36 no.3
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• pp.75-87
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• 2018

The purpose of this study is to investigate conservation and management methods of the Joseon Royal Tombs under the Eastern District Management Office. Through the literature survey, we understood the process of change of Joseon royal tombs, and through field surveys and interviews, we understood the status of the interior and the surrounding area. In this process, topography, land use and flow of human traffic, architecture and stone objects, water system, historical forests, and facilities were set as the main evaluation indicators. Urbanization has damaged the original terrains of Royal Tombs as national roads, buildings and facilities have constructed in the inner and outer area of Joseon Royal Tombs. Construction of underground passage, land purchase, relocation and demolition of the buildings are required for the conservation of the Royal Tombs area, and then it is necessary to recover the original terrain. In the case of land use and pathways, there are many disconnection of the original ritual circulation, they should be maintained to remind the sacred atmosphere of the royal tomb. And It is necessary to collect accurate information on the lost buildings and stoneworks through literature survey and excavation investigation, and that investigations should be lead to the exposure or restoration of the ruins. Historical forests require periodic and ongoing monitoring and management, and it is necessary to establish new entrance area and appropriate facilities following the long-Term conservation and management plan. These plans should be classified into short, medium and long-Term projects according to urgency and securing financial resources with a long perspective to implement continuous and systematic projects.