• 제목/요약/키워드: Momordin Ic

검색결과 7건 처리시간 0.026초

지부자 활성성분이 D-Galactosamine 투여에 의한 흰쥐의 간손상에 미치는 영향 (The Hepatoprotective Effect of Active Compounds of Kochiae fructus on D-Galactosamine-Intoxicated Rats)

  • 김나영;이정숙;박명주;이경희;김석환;최종원;박희준
    • 한국식품영양과학회지
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    • 제33권8호
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    • pp.1286-1293
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    • 2004
  • 지부자(Kochiae Fructus)의 생리활성물질 검색 및 간손상에 미치는 영향을 연구할 목적으로 실험동물에 지부자 추출물을 경구 투여한 후 GaIN으로 간손상을 유발하여 혈액 및 간조직의 생화학적 변화를 관찰하고 free radical의 생성계와 해독계의 활성에 미치는 영향을 검토한 결과는 다음과 같다. GaIN 단독투여군은 대조군에 비하여 AST와 ALT가 증가하였으나, KFB, oleanolic acid, momordin Ic 투여군에서는 GaIN 단독투여군에 비해 유의적 감소를 보였다. 간조직의 지질과산화 함량은 GaIN 단독투여군이 대조군과 비교하여 증가하였고, KFM 200-GaIN군, KFB 200-GaIN군, momordin Ic 30-GaIN군과 oleanolic acid 30-GaIN군은 대조군에는 미치지 못하였으나 GaIN 단독투여군에 비해 현저히 감소하였다. XO, AO의 활성은 대조군보다 GaIN 단독투여군에서 유의적으로 증가하였으며, KFB 200-GaIN군, momordin Ic 30-GaIN군과 oleanolic acid 30-GaIN군의 XO와 AO의 활성은 GaIN 단독투여군보다 낮게 나타났다. 간조직 중의 GSH농도는 GaIN 단독투여군이 대조군에 비해 현저히 감소하였고, KFB 200-GaIN군과 oleanolic acid 30-GaIN군은 GaIN 단독투여군과 비교시 증가를 보였고, momordin Ic 30-GaIN군은 대조군에 가깝게 회복되었다. GaIN 단독투여군의 ${\gamma}$-GCS와 GR의 활성은 대조군에 비하여 유의한 감소를 보였고, momordin Ic 30-GaIN군의 ${\gamma}$ -GCS의 활성은 대조군에는 미치지 못하였지만 GaIN 단독투여군에 비해 유의하게 개선되었다. KFM 200-GaIN군, KFB 200-GaIN군, momordin Ic 30-GaIN군과 oleanolic acid 30-GaIN군의 GR 활성은 GaIN 단독투여군보다 유의한 증가를 보였다. GST활성은 GaIN 단독투여군이 대조군에 비하여 현저한 감소를 나타내었고, KFM200-GaIN군, KFB 200-GaIN군, momordin Ic 30-GaIN군과 oleanolic acid 30-GaIN군은 대조군 수준에는 못미쳤으나 GaIN 단독투여군보다 통계적으로 유의한 증가를 관찰할 수 있었다. SOD, catalase 및 GSH-Px의 활성은 대조군에 비하여 GaIN 단독투여군에서 감소를 보였고, SOD와 catalase 활성은 KFM, KFB와 oleanolic acid의 투여로 GaIN 단독투여군보다 높게 나타났다. 특히 momordin Ic 30-GaIN군의 SOD 활성은 대조군에 가깝게 개선되었다. GSH-Px의 활성은 KFM 200-GaIN군, KFB 200-GaIN군과 oleanolic acid 30-GaIN군은 대조군 수준에는 미치지 못하였으나 GaIN 단독투여군에 비해 현저히 증가하였고, 특히 momordin Ic 30-GaIN군은 대조군에 가깝게 증가되었다. 이상의 결과를 종합하여 볼 때 지부자로부터 분리한 momordin Ic가 GSH 농도를 증가시키고 활성산소 해독계에 관여하는 효소의 활성을 증가시킴으로서 GaIN으로 인한 간손상을 완화시키는 것으로 사료되어진다.

Anti-Rheumatoid Arthritis Effect of the Kochia scoparia Fruits and Activity Comparison of Momordin Ic, its Prosapogenin and Sapogenin

  • Choi, Jongwon;Lee, Kyung-Tae;Jung, Hyun-Ju;Park, Hee-Sun;Park, Hee-Juhn
    • Archives of Pharmacal Research
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    • 제25권3호
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    • pp.336-342
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    • 2002
  • MeOH extract of Kochia scoparia was fractionated into $CHCl_3-$, EtOAc- and BuOH extracts and the last fraction were hydrolyzed by 3%-NaOH ($MeOH-H_2O$) to compare the bioactivities on antinociceptive and anti-inflammatory effects. Silica gel column chromatography of BuOH fraction afforded a large amount of $3-Ο-{\beta}-D-xylopyranosyl {\;}(1{\rightarrow}3)-{\beta}-D-glucuronopyranosyl$ oleanolic acid (momordin Ic, 4) and that of acid hydrolysate of BuOH fraction gave $3-Ο-{\beta}-D-glucuronopyranosyl oleanolic$ acid (momordin Ib, 3), its 6'-Ο-methyl ester (2) and oleanolic acid (1). Silica gel column chromatography of alkaline hydrolysate afforded a large amount of 4. MeOH extract and both EtOAc- and BuOH fractions were active in the rheumatoidal rat induced Freund's complete adjuvant reagent (FCA) whereas $CHCl_3$ fraction was inactive. Compound 1 and 4 showed significant activities in the same assay but oleanolic acid 3-Ο-glucuronopyranoside (3) showed no activity. These fashions were also observed in carrageenan-induced edema of the rat and in the antinociceptive activity tests undertaken in hot plate- and writhing methods. These results suggest that momordin Ic and its aglycone, oleanolic acid, could be active principles for rheumatoid arthritis.

Anti-proliferative and Apoptosis Inducing Effect of Momordin I on Oral Carcinoma (KB) Cells

  • Seo, Kyeong-Seong;Kim, Jeong-Hee;Kim, Yeo-Gab
    • International Journal of Oral Biology
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    • 제32권3호
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    • pp.113-118
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    • 2007
  • Treatment of oral cancers with chemotherapeutic agents become evaluated as an effective method to reduce cancer cell proliferation. Anti-proliferative and anti-oral cancer activities of momordin I on oral cancer cells were evaluated in this study. Momordin I was originally purified from a natural product, Ampelopsis radix and showed the antiproliferative activity against oral carcinoma, KB cells. Obtained $IC_{50}$ value was approximately $10.4{\mu}g/ml$. Time-and dose-dependent chromosomal DNA fragmentations were observed in momordin I-treated KB cells. Flow cytometry analysis showed time-dependent apoptotic cell appearance after treatment of momordin I. Approximately 18.6% apoptotic cells were observed at 72 hours after $20{\mu}g/ml$ of momordin I treatment. These observation were consistent with the results obtained in DNA fragmentation analysis. These data suggest that momordin I has anti-proliferative effect and induces cell death in KB cells through apoptosis.

천연약제 Momordin의 구강암(KB) 세포주에 대한 항암작용기전에 관한 연구 (STUDIES ON ANTICANCER EFFECT OF MOMORDIN ON ORAL CARCINOMA (KB) CELLS)

  • 서경성;김여갑
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제27권3호
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    • pp.209-213
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    • 2001
  • Treatment of oral cancers with chemotherapeutic agents are evaluated as an effective method for remission to reduce cancer proliferation nowadays. But, minimization of side-effects such as bone marrow suppression, gastrointestinal toxicity and renal damage is another problem to be solved. Thus, a possible approach to develop a clinically applicable chemotherapeutic agents is to screen anticancer activity among traditional medicinal plants which have been used for thousands of years with very low side-effects in orient. In this study we focused on anti-oral cancer activities of momordin, which was medicinal plant extracts that was revealed anticancer activities, on KB cell(oral cancer cell). The results were as follow : 1. Momordin showed the excellent anti-oral cancer activity against KB cells. Obtained IC50 value of Momordin was $10.4{\mu}g/ml$. 2. When KB cells were treated with Momordin, dose and time dependent DNA fragmentation of KB cells were observed. DNA fragmentation was initiated on three days at the concentration of $20{\mu}g/ml$ Momordin. 3. Flow cytometry showed dose-dependent apoptotic cell increase of KB cells on Momordin. 18.55% apoptotic cell were observed up to 72 hours at the concentration of $20{\mu}g/ml$ of Momordin. 4. Momordin induced nonspecific apoptosis without specific cell cycle arrest. 5. Through MTT assay, DNA fragmentation assay and flow cytometric analysis. anticancer effect of Momordin against KB cell was induce of apoptotic cell death.

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Inhibitory Effects of Momordin I Derivatives on the Formation of Fos-Jun-AP-1 DNA Complex

  • Lee, Ju-hyung;Park, Chi-Hoon;Kim, Wook-Hwan;Hwang, Yun-Ha;Jeong, Kyung-chae;Yang, Chul-Hak
    • Bulletin of the Korean Chemical Society
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    • 제27권4호
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    • pp.535-538
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    • 2006
  • In our previous studies, we have observed that curcumin and momordin I isolated from Ampelopsis radix inhibit the formation of Fos-Jun-activation protein-1 (AP-1) DNA complex. We have screened more effective compounds which have a 5-membered ring framework like momordin I and have modified disaccharide or carboxylic acid portions in momordin I. We synthesized momordin I derivatives according to the published method with slight modification. Synthetic momordin I derivatives showed remarkable inhibitory activities on Fos-Jun-AP-1 DNA complex formation results in in vitro assays. The $IC_{50}$ values of momordin I derivatives were about 4.0 $\mu$M in an electrophoretic mobility shift assay (EMSA). This value is about 125 times higher than that of curcumin and about 12 times higher than that for curcumin derivative C1, and moreover about 30 times higher than that for momordin I. We found momordin I derivatives (a) and (b) are the strongest inhibitory compound for Fos-Jun-AP-1 DNA complex formation.

호박$(Cucurbita\;moschata\;D_{UCHESNE})$잎에서 리보즘불활성화 단백질의 분리 및 특성 (Purification and Properties of Ribosome-inactivating Proteins from the Leaves of $Cucurbita\;moschata\;D_{UCHESNE}$)

  • 이시명;김영태;황영수;조강진
    • Applied Biological Chemistry
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    • 제40권5호
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    • pp.375-379
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    • 1997
  • 리보즘불활성화 단백질(Ribosome-inactivating protein, RIP)을 생성하는 식물을 탐색하여 그중 호박$(Cucurbita\;moschata\;D_{UCHESNE})$ 잎에서 ammonium sulfate 침전, DE 52-Cellulose, S-Sepharose, FPLC Superose 12 HR, FPLC Mono-S column chromatography에 의하여 ribosome-Inactivating 활성이 있는 단백질(PR 1, PRIP 2)을 분리하였다. 정제된 단백질의 분자량은 SDS-PAGE에서 약 31,000과 30,500인 염기성 단백질로서, 특히 PRIP 1은 열에도 안정하여 $50^{\circ}C$에서 30분간 처리한 경우에도 활성이 유지되었다. 이 단백질들의 ribosome-inactivating 활성을 in vitro translation system에서 측정한 결과 50% 활성저해농도 $(IC_{50})$는 PRIP 1은 0.82nM, PRIP 2은 0.79 nM이었다. PRIP 1과 PRIP 2의 N-말단부분의 아미노산 서열을 분석하여, 이미 밝혀진 리보즘불활성화 단백질들과 아미노산서열의 유사성을 분석해 본 결과, PRIP 1은 Luffa cylindrica에서 분리된 Luffin B 및 Trichosanthes kirilowii Maximowicz에서 분리된 Trichokirin과, PRE 2은 Momordia charantia에서 분리된 Momordin II 및 MAP 30과 유사성이 매우 높았다.

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