• Journal of Veterinary Science
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• 제22권4호
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• pp.50.1-50.10
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• 2021

Background: Bovine papillomatosis is a type of proliferative tumor disease of skin and mucosae caused by bovine papillomavirus (BPV). As a transboundary and emerging disease in cattle, it poses a potential threat to the dairy industry. Objectives: The aim of this study is to detect and clarify the genetic diversity of BPV circulating in dairy cows in Xinjiang, China. Methods: 122 papilloma skin lesions from 8 intensive dairy farms located in different regions of Xinjiang, China were detected by polymerase chain reaction. The genetic evolution relationships of various types of BPVs were analyzed by examining this phylogenetic tree. Results: Ten genotypes of BPV (BPV1, BPV2, BPV3, BPV6, BPV7, BPV8, BPV10, BPV11, BPV13, and BPV14) were detected and identified in dairy cows. These were the first reported detections of BPV13 and BPV14 in Xinjiang, Mixed infections were detected, and there were geographical differences in the distribution of the BPV genotypes. Notably, the BPV infection rate among young cattle (< 1-year-old) developed from the same supply of frozen sperm was higher than that of the other young cows naturally raised under the same environmental conditions. Conclusions: Genotyping based on the L1 gene of BPV showed that BPVs circulating in Xinjiang China displayed substantial genetic diversity. This study provided valuable data at the molecular epidemiology level, which is conducive to developing deep insights into the genetic diversity and pathogenic characteristics of BPVs in dairy cows.

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.56-69
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• 2022

Silk is a unique natural biopolymer with outstanding biocompatibility, high mechanical strength, and superior optical transparency. Due to its excellent properties, silk has been widely reported as an ideal biomaterial for several biomedical applications. Recently, fluorescent silk protein, a variant of native silk, has been reported as a biophotonic material with the potential for bioimaging and biosensing. Despite the realization of fluorescent silk, the traditional degumming process of fluorescence silk is crude and often results in fluorescence loss. The loss of fluorescent properties is attributed to the sensitivity of silk fibroin to temperature and solvent concentration during degumming. However, there is no comprehensive information on the influence of these processing parameters on fluorescence evolution and decay during fluorescent silk processing. Therefore, we conducted a spectroscopic study on fluorescence decay as a function of temperature, concentration, and duration for fluorescent silk cocoon degumming. Sodium carbonate solution was tested for degumming the fluorescent silk cocoons with different concentrations and temperatures; also, sodium carbonate solution is combined with Alcalase enzyme and triton x-100 to find optimal degumming conditions. Additionally, we conducted a molecular dynamics study to investigate the fundamental effect of temperature on the stability of the fluorescent protein. We observed degumming temperature as the prime source of fluorescent intensity reduction. From the MD study, fluorescence degradation originated from the thermal agitation of fluorescent protein Cα atoms and fluctuations of amino acid residues located in the chromophore region. Overall, degumming fluorescent silk with sodium carbonate and Alcalase enzyme solution at 25 ℃ preserved fluorescence.

• Fisheries and Aquatic Sciences
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• 제9권2호
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• pp.70-74
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• 2006

DNA sequence-based typing is considered a robust tool for the discrimination of dinoflagellate species because of the availability of extensive rDNA sequences. Here, we present a rapid, cost-effective DNA-sequencing technique for various PCR products. This sequencing strategy relies on 'nested' or 'tailed' primer labeled with near-infrared dye, and uses a minimal volume of unpurified PCR product (ca. $5{\mu}L$) as the DNA template for sequencing reactions. Reliable and accurate base identification was obtained for several hundred PCR fragments of rRNA genes. This quick, inexpensive technique is widely applicable to sequence-based typing in clinical applications, as well as to large-scale DNA sequencing of the same genomic regions from related species for studies of molecular evolution.

• Journal of Photoscience
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• 제9권2호
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• pp.142-145
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• 2002

A strict anaerobe, Prevotella melaninogenica is highly sensitive to oxidative stress. Oxidative stress such as exposure to oxygen or addition of hydrogen peroxide, increased 8-hydroxydeoxyguanosine (80HdG), a typical of oxidative DNA damage, and decreased the bacterial cell survival rate. We could detect the generation of reactive oxygen species in P. melaninogenica after exposure to oxygen. UVA irradiation also increased 80HdG in the bacterium. On the other hand, such oxidative stress did not increase 80HdG in a facultative anaerobe. These findings suggest that P. melaninogenica is a suitable material to study the biological effects of oxidative stress, to evaluate antioxidants, and to study the effects of oxygen or reactive oxygen species on molecular evolution.

• Journal of Photoscience
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• 제9권2호
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• pp.82-85
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• 2002

33kDa extrinsic protein, an important protein in oxygenic photosynthesis, was known to have no fixed configuration in solution. At 20$\^{C}$ and pH 6, 33kDa extrinsic protein showed changes of free energy of -14.6 kJ/mor$\^$-1/ and of standard volume of -120mL/mol, respectively, with increase of hydrostatic pressure, comparatively lower than for most proteins. NBS modification of Trp241 in 33kDa extrinsic protein dramatically changes the secondary protein structure, its affinity to photosystem II as well as photosynthetic oxygen evolution. The relationship between structural change and transport of oxygen, water and proton is deserved a further study.

• 대한미생물학회지
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• 제35권5_6호
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• pp.349-349
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• 2000

• Animal Systematics, Evolution and Diversity
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• 제29권1호
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• pp.18-22
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• 2013

A recent study on the mitochondrial genetic variation of the Carassius auratus population in South Korea suggested that there are 3 distinct mitochondrial lineages in the country, and that they are geographically separated between westward rivers and southward rivers, respectively. In this study, the population genetic structure of amplified fragment length polymorphism (AFLP) of Carassius auratus was investigated. The results of analysis of molecular variance (AMOVA) supported the geographic distinction between westward and southward river populations, but only 3.66% of total genetic variance lies among these populations. The panmicticity of the AFLP genetic variation is backed up by the results of the neighbor-joining dendrogram drawn from a linearized pairwise $F_{ST}$ matrix and Bayesian clustering analysis. The discordance of genetic structure between mitochondrial and AFLP genetic variation may come from difference in effective population size between these markers and/or gene flow between westward and southward river populations through river capture events.

• Animal Systematics, Evolution and Diversity
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• 제27권3호
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• pp.205-212
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• 2011

Platorchestia monodi (Mateus et al., 1986), the beach hopper, commonly lives in sea shores and was previously reported only from the Atlantic regions, such as Mid-Atlantic islands, Brazilian coast, and Israel. We present the first record of this species in the Pacific region. The present species can be clearly distinguished from related species by the shapes of gnathopods 1-2 and development of sexual dimorphism in antenna 2 and pereopod 7. Descriptions of the diagnostic characteristics of the species are provided on the text. In addition, this paper also provided the partial sequence of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene of the species for its molecular characteristics.

• 대한화학회지
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• 제24권6호
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• pp.463-468
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• 1980

Tris(hydroxyethyl) phosphate, ethylphosphoramidic dichloride, N,N-diethylphosphoramidic dichloride, bis(hydroxyethyl) N-ethylphosphoramidate 나 bis(hydroxyethyl) N,N-diethylphosphoramidate를 합성하고 특성을 고찰하였다. Phosphate와 두종류의 phosphoramidate는 감압하에서 가열하면 ethylene glycol이 빠지면서 중합반응이 일어났으며 이들 화합물의 mass 스펙트라에서는 분자이온피이크를 관찰할 수 없었다. 또한 ethylphosphoramidic dichloride도 180$^{\circ}C$에서 HCl이 발생되면서 중합체로 변하였다. IR스펙트라에서는 P=O strectching의 특성밴드가 $1,300{\sim}1,200 cm^{-1}$에서 나타났다.

• Animal Systematics, Evolution and Diversity
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• 제33권3호
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• pp.195-199
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• 2017

A sea cucumber was collected from Gonghyeonjin in the East Sea of Korea at a depth of 50 m on 22 June 2011 and was identified as Psolus phantapus (Strussenfelt, 1765). This species belongs to the family Psolidae of the order Dendrochirotida based on morphological characteristics and mitochondrial cytochrome c oxidase subunit I sequence analysis. Psolus phantapus, which widely distributes in the Arctic and North Atlantic Oceans, is newly recorded in the Korean fauna. Two Psolus species including the previously reported P. squamatus are recorded in the East Sea of Korea.