• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.3
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• pp.399-408
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• 1998

The effect of low molecular alginates feeding on the cholesterol levels and fatty acid compositions of rat serum and liver lipid were investigated. After one week basal diet feeding, four week old Sprague-Dawley male rats were fed with water soluble and acid $\cdot$alkali soluble alginate extracted from sea mustard (Undaria pinnatifida) and giant kelp (Macrocystis pyrifera), and their low molecular alginates prepared by the HCl partial hydrolysis. The feeding efficiency of the alginate fed group was ranged in 0.37$\~$0.44, which was 0.03$\~$0.05 lower than that of the basal diet group. Also, there was much less increase of liver weight in the alginate fed group. The water soluble alginate showed more significant effect in reducing the total cholesterol, free cholesterol, LDL-cholesterol, triglyceride and phospholipid of serum and liver lipid than the acid$\cdot$alkali soluble alginate. The effect was much better with low molecular alginate (reducing effect by the low-molecularization : Water soluble alginate - serum lipid; total cholesterol $59\%$, free cholesterol $65\%$, LDL-cholesterol $96\%$, triglyceride $50\%$, and phospholipid $36\%$. liver lipid: total cholesterol $4\%$, free cholesterol $62\%$, LDL-cholesterol $44\%$, triglyceride $33\%$, and phospholipid $44\%$. acid$\cdot$alkali soluble alginate - serum lipid; total cholesterol $52\%$: free cholesterol $97\%$, LDL-cholesterol $78\%$ triglyceride $32\%$, and phospholipid $64\%$. liver lipid; total cholesterol $11\%$, free cholesterol $12\%$, LDL-cholesterol $10\%$, triglyceride $27\%$, and phospholipid $21\%$). The effect of low molecular alginate feeding on the fatty acid composition of serum and liver lipid reflects the remarkable increase of polyenoic acid, over $44\%$ in serum lipid and about $70\%$ in liver lipid, comparing to the cholesterol fed group. The overall results indicated that feeding of low molecular alginates improves physiological function of rats by changing the serum and liver lipid composition.

• Food Science and Preservation
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• v.4 no.3
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• pp.301-309
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• 1997

This study was conducted to examine morphological characteristics and the content of cell wall polysaccharides of Brassica campestris var. pekinensis(baechu). First of all, the variety of scientific name and naming of parts of baechu in the literatures of kimchi showed, which will unify marks. So, we propose not so much mid-rib and leaf blade of baechu leaf as white part and green part, respectively. On the other hand, the forms of vessel elements of white part in baechu consist in ring, sclariform and reticulate thickening. The proximate compositions and contents of cell wall polysaccharides of baechu has significant differences between its cultivars. The cell wall pectin from baechu exhibited four peals with molecular weights of 2,000,000, about 100,000 and less than 10,000 by gel filteration chromatography and hemicellulose did two peaks with molecular weights of 2,000,000 and 10,000.

• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.4
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• pp.335-341
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• 1990

Nuclei proteins were purified from chick liver to homogeneity by means of acid extraction CM Sephadex c 25 column chromatography and Bio Rex 70 column chromatography, The molecular weight of liver Nuclei proteins 1 and 2 as estimated by electrophoresis on SDS-polycrylamide gel are 29000 and 27,000 respectively. These molecular weights are identical with those of Nuclei Proteins 1 and 2 isolated from chick erythrocyte. The liver and erythrocyte Nuclei Proteins also co-migrated in acetic acid-urea gel electrophoresis. Furthermore the anti-sera raised against liver Nuclei Proteins 1 and 2 cross-reacted with erythrocyte Nuclei Proteins 1 and 2 respectively, However the amino acid compositions of liver Nuclei Prooteins 1 and 2 were found to be different from those of corresponding erythrocyte Nuclei proteins ; the contents of serine and proline in liver Nuclei proteins were higherocyte Nuclei proteins ; the contents of serine and proline in liver Nuclei protesins were higher than those in erythrocyte Nuclei proteins while the content of lycsine in liver Nuclei proteins was lower than the erythrocyte Nuclei proteins, These results suggest that in spite of similarities in many respects the liver and erythrocyte Nuclei proteins in chicks and different proteins.

• Journal of Sericultural and Entomological Science
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• v.41 no.3
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• pp.211-215
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• 1999

The pure-separation of calcium chloride-treated fibroin hydrolysates could be carried out using gel filtration chromatography. Also, the effect of its enzymatic hydrolysis was investigated in order to find out the enhancement of their functionality. The average molecular weight(Mw), solubility and free amino acid compositions of three hydrolysates samples (calcium chloride, calcium chloride-flavourzyme and calcium chloride-thermoase)were measured to compare their characteristics. The molecular weight of calcium chloride hydrolysate was about Mw 46,800 and it can be reduced to Mw 12,500 and 1,070 upon the enzymatic hydrolysis by flavourzyme and thermoase, repectively. A solubility of calcium chloride-treated samples shows about 60% while calcium chloride/enzyme-treated samples are perfectly soluble (100% solubility). The total amino acid composition of calcium chloride enzymatic hydrolysates are much higher than that of calcium chloride hydrolysate.

• Bulletin of the Korean Chemical Society
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• v.15 no.5
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• pp.399-404
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• 1994

The non-Newtonian viscosities (the specific or intrinsic viscosities) of poly(L-proline) (PLP, $M_v$=19,000 and 32,000) in various mixed-solvent systems like water-propanol and acetic acid-propanol of varying compositions were measured during the reverse mutarotation (Form II ${\rightarrow}$Form I) by the application of external pressure (up to 4.5 psi). The non-Newtonian viscosity effect was found to be larger in acetic acid-propanol system than in water-propanol system and to somewhat decrease during the reverse mutarotation at a given solvent system. The non-Newtonian viscosity behavior of PLP in aqueous salt ($CaCl_2$) solution was also studied, from which it was found that the degree of the non-Newtonian effect decreased with increasing salt concentration, and increased with increasing PLP molecular weight. These findings could be explained in terms of conformational changes of PLP in solution (like the helix-helix or helix-coil transition) involved.

• ALGAE
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• v.30 no.3
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• pp.183-195
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• 2015

The dinoflagellate genus Pelagodinium is genetically classified in distinct sub-clades and subgroups. However, it is difficult to determine whether this genetic diversity represents intra- or interspecific divergence within the genus since only the morphology of the type strain of the genus Pelagodinium, Pelagodinium bei, is available. An isolate associated with the genus Pelagodinium from Shiwha Bay, Korea, was recently cultured. This isolate was clustered with 3 to 4 strains from the Atlantic Ocean, Mediterranean Sea, and Indian Ocean. This cluster was distinct from the subgroup more closely associated with P. bei. The morphology of the isolate was analyzed using optical and scanning electron microscopy and was almost identical to that of P. bei except that this isolate had two series of amphiesmal vesicles (AVs) in the cingulum, unlike P. bei that has one series. When the pigment compositions of the isolate and P. bei were analyzed using high-performance liquid chromatography, these two strains had peridinin as a major accessory pigment and their pigment compositions were almost identical. In addition, the swimming behaviors of these two strains were very similar. The reexamination of the type culture of P. bei revealed two series in the cingulum as for the isolate. The new findings on the number of series of AVs in the cingulum, the pigment composition, and the swimming behaviors suggest that P. bei and the isolate are conspecific despite their genetic divergence. This study provides a basis to further understand the molecular classification within Pelagodinium combining genetic, morphological, pigment, and behavioral data.

• Bulletin of the Korean Chemical Society
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• v.32 no.7
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• pp.2358-2368
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• 2011

Density functional theory (DFT) and time-dependent density functional theory (TDDFT) calculations, employing the B3LYP method and the LANL2DZ, 6-31G$^*$(LANL2DZ for Tc), 6-31G$^*$(cc-pVDZ-pp for Tc) and DGDZVP basis sets, have been performed to investigate the electronic structures and absorption spectra of the technetium-99m-labeled methylenediphosphonate ($^{99m}Tc$-MDP) complex of the simplest diphosphonate ligand. The bonding situations and natural bond orbital compositions were studied by the Mulliken population analysis (MPA) and natural bond orbital (NBO) analysis. The results indicate that the ${\sigma}$ and ${\pi}$ contributions to the Tc-O bonds are strongly polarized towards the oxygen atoms and the ionic contribution to the Tc-O bonding is larger than the covalent contribution. The electronic transitions investigated by TDDFT calculations and molecular orbital analyses show that the origin of all absorption bands is ascribed to the ligand-to-metal charge transfer (LMCT) character. The solvent effect on the electronic structures and absorption spectra has also been studied by performing DFT and TDDFT calculations at the B3LYP/6-31G$^*$(cc-pVDZ-pp for Tc) level with the integral equation formalism polarized continuum model (IEFPCM) in different media. It is found that the absorption spectra display blue shift in different extents with the increase of solvent polarity.

• Polymer(Korea)
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• v.39 no.1
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• pp.13-22
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• 2015

A new methacrylate copolymer that includes chalcone as a side group, poly(4-methacryloyloxyphenyl-4'-methoxystyryl ketone-co-styrene) was synthesized by free radical copolymerization. FTIR and $^1H$ NMR spectroscopic techniques were used to characterize the homopolymers and copolymers. The copolymerizations were carried out to high conversions. Copolymer compositions were established by $^1H$ NMR spectra analysis. The monomer reactivity ratios for copolymer system were determined by the linearized Kelen $T{\ddot{u}}d{\ddot{o}}s$, and Extended Kelen $T{\ddot{u}}d{\ddot{o}}s$ methods and a non-linear least squares method. The molecular weights and polydispersity index of copolymers were measured by using the gel permeation chromatography (GPC). The effect of copolymer compositions on their thermal behavior were studied by differential scanning calorimetry and thermogravimetric analysis methods. The optical properties of the resulting copolymer were also investigated.

• Food Science of Animal Resources
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• v.38 no.1
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• pp.14-25
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• 2018

Chemical compositions, trypsin inhibitory activity, and gelling properties of albumen from duck egg during salting of 30 days were studied. As the salting time increased, moisture content decreased, the salt content and surface hydrophobicity increased (p<0.05). Trypsin inhibitory activity and specific activity were continuously decreased throughout the salting time of 30 days (p<0.05). This coincided with the decrease in band intensity of inhibitor with molecular weight of 44 kDa as examined by inhibitory activity staining. Nevertheless, no differences in protein patterns were observed in albumen during the salting of 30 days. Based on texture profile analysis, hardness, springiness, gumminess, chewiness, and resilience of albumen gel decreased with increasing salting time. Conversely, salted albumen gels exhibited higher cohesiveness and adhesiveness, compared to those of fresh albumen. Scanning electron microscopic study revealed that gel of salted albumen showed the larger voids and less compactness. In general, salting lowered trypsin inhibitory activity and gelling property of albumen from duck egg to some extent. Nevertheless, the salted albumen with the remaining inhibitor could be an alternative additive for surimi or other meat products to prevent proteolysis.

• BMB Reports
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• v.35 no.5
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• pp.459-464
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• 2002

Fusogenic liposomes that incorporate Sendai virus envelope proteins, so-called Sendai virosomes, have been developed for in vitro and in vivo genetic modification of animal cells. In this study, several different virosomes of varying lipid compositions were formulated and their in vitro gene-transfer efficiencies compared. The virosomes were prepared by quantitative reconstitution of the Sendai envelope, fusion (F) and hemagglutinin-neuraminidase (HN) proteins into liposomal vesicles. Virosomes that contained luciferase reporter genes were tested in 293 transformed human kidney cells. F/HN-virosomes that were prepared with an artificial Sendai viral envelope (ASVE-virosomes) or phosphatidylserine (PS-virosomes) exhibited an 8- or 6-fold higher gene-transfer efficiency than cationic liposomes that were made with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). F/HN-virosomes that were prepared with phosphatidic acid (PA-virosomes) instead of PS were less efficient in gene transfer than either ASVE- or PS-virosomes. In addition, the genetransfer capability of ASVE- and PS-virosomes was maximal at a $Ca^{2+}$ concentration of 510 mM. These results suggest that the incorporated lipid components significantly affect the in vitro gene transfer that is mediated by Sendai F/HN-virosomes.