• Asian-Australasian Journal of Animal Sciences
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• v.9 no.2
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• pp.127-132
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• 1996

Effects on feed intake, liveweight gain and economic benefits of supplementing lambs with urea molasses blocks, were studied. Forty eight crossbred lambs were divided into 6 groups and assigned randomly to grazing on native pasture (CONT) or along with supplements of Commercial ration (COM) and urea molasses blocks (UMBs) containing two levels of cement and calcium oxide as a binding agent. Analysis of variance revealed highly significant (p<0.01) differences in dry matter (DMI, g/day), crude protein (CPI, g/day) and metabolizable energy (MEI, MJ/day) intakes. Differences in liveweight gain (LWG, g/day), feed conversion ratio (FCR) and net economic benefit of supplementation were also highly variable. The intake of DM, CP and ME varied from 974 to 1002, 66-70 and 7.6-8.4 in lambs supplemented with UMBs, significantly (p<0.01) greater than 848, 52.5 and 5.6 in lambs supplemented with COM or FCR and net economic benefits (54.3; 57.8; 17.1 and 1.96; 2.4) in lambs supplemented with COM and UMB-2, were CONT or supplemented with UMB-1, UMB-3 and UMB-4 respectively. Factors responsible for differences in feed intake, liveweight gain and economic benefits, are discussed.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.1
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• pp.39-44
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• 1992

Twelve male crossbred calves of 18 months of age were divided into two groups of six each. Animals in both the groups were fed wheat straw ad lib. However, animals in group II had free access to urea molasses mineral block (UMMB) lick. Straw DM intake (kg/100 kg B. Wt. and $g/w^{0.75}\;kg$) was significantly (p < 0.01) higher in group II ($1.95{\pm}0.06$; $75.55{\pm}1.79$) as compared to group I ($1.27{\pm}0.08$; $48.77{\pm}2.43$). Straw DM digestibility coefficient was not significantly different in groups I and II. However, DOMI (kg/100 kg B. Wt.) was significantly (p < 0.01) higher in group II ($0.986{\pm}0.05$) as compared to group I ($0.615{\pm}0.03$). Digestibility coefficient of DM, OM and CP were significantly higher in group II as compared to group I. However, digestibility coefficients of EE, CF and NFE were non-significantly different between the two groups. Animals in group II exhibited significantly (p < 0.01) higher and positive N, Ca and P balances as compared to group I which exhibited negative balances. Total-N, ammonia-N and urea-N in the blood plasma of animals in group II were significantly (p < 0.01) higher as compared to group I.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.196-202
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• 1992

Zoogloea ramigera 115 was selected for the production of viscous microbial polysaccharide for bioflocculants usage. Batch, fed-batch, and continuous culture processes were examined with regard to the high biopolymer production. Several carbon sources were tested, including glucose, lactose, molasses, and cheese whey. The C/N ratio of 90 was most effective for biopolymer production from glucose, while the C/N ratios of 30 for lactose and 60 for both molasses and cheese whey substrate gave a maximum production. Fed batch culture proved more effective to increase final biopolymer concentration than batch culture. Continuous fermentation with two stages modifying C/N ratio increased the productivity. The production rates were a maximum at dilution rate of 0.048 $hr^{-1}$ for molasses and at 0.096 $hr^{-1}$for cheese whey.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.43-52
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• 1991

The overall objective of this research was to evaluate the effects of spraying sulfuric acid solution, and adding corn meal or molasses to reed canarygrass silage on digestibility of dry matter (DM) and fibrous components in ruminant. DM content of molasses or corn meal treated silages were higher than control. Whereas, sulfuric acid treated silage showed lower DM content compared with untreated control. Acid detergent fiber content was similar among treatments, but neutral detergent fiber (NDF) and hemicellulose concentration were higher in treated silages than those of control. Organic acids content were also increased in treated silages. In contrast, silage pH were lower in treated silages. Treated silages increased both rate and extent of DM and NDF in situ digestibility compared to control at all incubation times. Especially, 7 % molasses and 0.4 % sulfuric acid treated silages increased DM and NDF digestibility significantly after 12 hours of incubation compared with control. In nitro DM and NDF digestibility showed similar trend to in situ incubation. Also treated silages(mo1asses or sulfuric acid treated) incubated in situ had higher particle-associated carboxymethylcellulase activity throughout the incubation except 72 hours.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.4
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• pp.361-368
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• 2012

The present study was conducted to develop persimmon peel, a by-product of dried persimmon manufacturing, as a feed additive via lactic acid bacteria fermentation. Pediococcus pentosaceus, Lactobacillus plantarum, and three strains of Leuconostoc mesenteroides were used as a starter culture in the solid state fermentation of persimmon peel, and antioxidant activity and total polyphenol content were assessed. Leuconostoc mesenteroides KCTC 3100 showed high antioxidant activity (p<0.05), whereas Pediococcus pentosaceus showed high total polyphenol content (p<0.05). These two strains were thus selected as starter culture strains. Glucose, sucrose and molasses were used as variables for optimization and a total 15 experimental runs were produced according to Box-Behnken design. Regarding significant effects of variables, molasses showed linear and square effects on antioxidant activity of persimmon peel fermentation (p<0.05). In conclusion, optimum concentrations of glucose, sucrose, and molasses were determined to be 4.2, 3.9 and 5.3 g/L, respectively, using a response surface model. Antioxidant activity was also improved 2.5 fold after optimization.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1583-1591
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• 2003

The influence of varying levels of urea and additives on nitrogen (N) retention and chemical composition of wheat straw was studied. The wheat straw was treated with 4, 6 and 8% urea and ensiled with 1.5, 2 and 2.5% of acetic or formic acid and 2, 4 and 6% of corn steep liquor (CSL) or acidified molasses for 15 days. The N content of wheat straw was significantly different across all treatments. The N content of urea treated wheat straw was increased with the increasing level of urea. The N content was higher in urea treated wheat straw ensiled with acetic or formic acid as compared to urea treated wheat straw ensiled without these organic acids. The N content of urea treated wheat straw was further enhanced when it was ensiled with CSL or acidified molasses. This effect was significant across all levels of urea used to treat the wheat straw. Nitrogen retention in urea treated wheat straw was decreased linearly as the urea level was increased to treat the wheat straw. The N content was increased linearly when higher levels of CSL or acidified molasses were used to ensile the urea treated wheat straw. Most of the N in urea treated wheat straw was held as neutral detergent insoluble N (NDIN). The NDIN content was increased linearly with the increasing levels of urea and additives. The neutral detergent fiber (NDF) contents were higher in urea treated wheat straw ensiled with acetic or formic acid as compared to urea treated wheat straw ensiled without additive. The NDF content further increased in urea treated wheat straw ensiled with CSL and acidified molasses. The entire increase in NDF content was because of fiber bound N. The hemicellulose content of urea treated wheat straw ensiled with CSL or acidified molasses was higher as compared to urea treated wheat straw ensiled with acetic or formic acid. The acid detergent fiber content of urea treated wheat straw ensiled with or without additives remained statistically non-significant. The cellulose contents of wheat straw was linearly reduced when urea level was increased from 4 to 6 and 8% to treat the wheat straw. This effect was further enhanced when urea treated wheat straw was ensiled with different additives. The results of the present study indicated that fermentable carbohydrates might improve the Nitrogen retention and bring the favorable changes in physiochemical nature of wheat straw. However, biological evaluation of urea treated wheat straw ensiled with fermentable carbohydrates is required.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.705-712
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• 2006

Influences of urea treated corncobs (UTC) ensiled with or without different additives on ruminal characteristics, in situ digestion kinetics, nutrient digestibility and nitrogen metabolism were examined in a $5{\times}5$ Latin square design using five ruminally cannulated buffalo bulls. Five iso-caloric and iso-nitrogenous diets were formulated to contain 30% dry matter (DM) from concentrate and 70% DM from 5% UTC ensiled without any additive (U) or with 5% enzose (EN), 5% acidified molasses (AM), 5% non-acidified molasses (NM) and 5% acidified water (AW), respectively. These diets were fed to buffalo bulls at 1.5% of their body weight daily. Ruminal $NH_3$-N concentration at 3 hours (h) post feeding was significantly higher in bulls fed U, NM and AW diets, however, at 6, 9 and 12 h post feeding it was significantly higher in bulls fed EN and AM diets. Ruminal total volatile fatty acids (VFA) and acetate concentrations were significantly higher with EM and AM diets compared with other diets at 3, 6, 9 and 12 h post feeding. Ruminal pH at 6 and 9 h post feeding was higher with EN and AM diets; however; it was notably lower with these diets at 3 h post feeding. Total ruminal bacterial and cellulolytic bacterial counts were higher in bulls fed EN and AM diets than in those fed the other diets. In situ ruminal DM and NDF degradabilities and total tract digestibilities were significantly higher with UTC ensiled with enzose and acidified molasses than those ensiled without any additive or other additives. Nitrogen balance was significantly higher in bulls fed EN and AM diets than those fed U, AW and NM diets. The UTC ensiled with enzose or acidified molasses resulted in better digestibility and N utilization than those ensiled without any additive, with non-acidified molasses and acidified water in buffaloes.

• Microbiology and Biotechnology Letters
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• v.29 no.4
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• pp.234-239
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• 2001

In order to maximize the RNA accumulation and biomass production is Saccharomyces cerevisiae MTY62, a high-content RNA yeast strain, batch and fed-batch cultures were performed. Among the feeding modes of fed-batch cultures examined, the intermittent feeding mode R\`(IFB-lV), in which 50 ml of 40% molasses and 20% com steep liquor (CSL) solution was intermittently fed for 5 times, resulted in the cell concentration of 33.8 g- dry cell weight/1 and the RNA concentration of 5221 mg-/l, and RNA content of 153 mg-RNA/g-dry cell weight. The constant fed-batch with feeding mode III (CFB-III), in which the feeding rate of 40% molasses and 20% CSL solution was stepwisely decreased from 48 mph (9-13 h), to 24 mph (13-21 h), and to 18 ml/h (21∼ 48 h), gave the highest cell concentration of 42.7 g-dry ceil weigh71 and R7IA concentration of 5536 mg-RNA/1, which were about 2.4-fold and 1.9-fold increased levels, respectively, compared to the results of batch culture. However, the RNA con- tent of 130 mg-RNA/g-dry cell weight of the fed-batch was lower than that of the batch culture (171 mg-RNA/g-dry cell weight) and other fed-batch cultures. When the specific growth rates in the fed-batch cultures were increased, the RNA contents increased. This result indicates that the RNA content is adversely proportional to the cell concen- tration. However, at the same specific growth rate, the RNA content was maintained at higher level in the intermit- tent fed-batch than in the constant fed-batch culture.

• Journal of Life Science
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• v.30 no.6
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• pp.522-531
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• 2020

The aim of this study was to establish the optimal medium composition for enhancing L(+)-lactic acid (LLA) production using response surface methodology (RSM). Lactobacillus paracasei SRCM201474 was selected as the LLA producer by productivity analysis from nine candidates isolated from kimchi and identified by 16S rRNA gene sequencing. Plackett-Burman design was used to assess the effect of eleven media components on LLA production, including carbon (glucose, sucrose, molasses), nitrogen (yeast extract, peptone, tryptone, beef extract), and mineral (NaCl, K₂HPO₄, MgSO₄, MnSO₄) materials. Glucose, sucrose, molasses, and peptone were subsequently chosen as promising media for further optimization studies, and a hybrid design experiment was used to establish their optimal concentrations as glucose 15.48 g/l, sucrose 16.73 g/l, molasses 39.09 g/l, and peptone 34.91 g/l. The coefficient of determination of the equation derived from RSM regression for LLA production was mathematically reliable at 0.9969. At optimum parameters, 33.38 g/l of maximum LLA increased by 193% when compared with MRS broth as unoptimized medium (17.66 g/l). Our statistical model was confirmed by subsequent validation experiments. Increasing the performance of LLA-producing microorganisms and establishing an effective LLA fermentation process can be of particular benefit for bioplastic technologies and industrial applications.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.997-1003
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• 2006

An experiment with twenty crossbred goat kids (male) of 2-3 months old, weighing about 12 kg was conducted to study the effect of feeding formaldehyde treated rape seed oil cake based diet supplemented with molasses on growth rate and histopathological changes of different organs. Goats were randomly divided into four groups of 5 animals each and were individually fed for a period of 120 days. The animals in group I (URC) and II (URCM) were fed concentrate mixture (CM-I) containing untreated rape seed oil cake (30%) while, the animals in group III (TRC) and IV (TRCM) were offered concentrate mixture (CM-II) containing formaldehyde treated rape seed oil cake. Further, molasses as energy source was additionally supplemented with the concentrate mixture at the rate of 8% of concentrate mixture on dry matter basis to animals in group II and IV. All the animals were maintained on roughage (Berseem hay:wheat straw = 2:1) and concentrate in 50:50 ratio. Average daily gain (g/d) of animals in group IV was significantly (p<0.05) higher than that in group I., but at par with group II and III. Feed conversion efficiency was also significantly (p<0.05) higher in group IV (10.14) than group I and II but at par with group III. The growth rate however increased by 50.2% in group IV showing more consistency in maintaining highest growth rate due to better balance of nutrients. At the end of four months of feeding trial, two animals from each group were sacrificed for histopathological study of different organs. Significant histopathological changes in liver, heart, lungs tissue of animals fed untreated rape seed oil cake diet were recorded which were totally absent in the organ of animals fed formaldehyde treated cake. The liver tissue of goats receiving control diet (containing untreated rape seed oil cake) were found to be associated with engorged central vein and blood vessels. Hepatocytes were swollen, pale and degenerated with cellular infiltration and fibrosis of portal areas. The muscles of heart were found to have intermyofibral edema. Emphysema accompanied by dilated and ruptured alveoli was also recorded in lung tissue. However, histopathological examination of various tissues of goats fed formaldehyde treated cake diet did not exhibit any degenerative changes. Additional supplementation of molasses with or without treated cake diet, apparently did not have any significant effect on ameliorating the above degenerative changes.