• BMB Reports
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• v.41 no.5
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• pp.369-375
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• 2008

Polygonatum cyrtonema Lectin (PCL), which is classified as a monocot mannose-binding lectin, has received great regards for its uniquely biological activities and potentially medical applications in cancer cells. This paper was initially aimed to study apoptosis of PCL on Hela cells. Thus, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method was carried out. Through observation of cell morphologic changes and Lactate dehydrogenase (LDH) activity-based cytotoxicity assays, PCL induced HeLa cell apoptosis in a dose-dependent manner. To further gain structural basis, multiple alignments, homology modeling and docking experiments were performed to analyze the correlation between its biological activities and mannose-binding sites. Eventually, considering docking data, chemical modification properties on the three mannose-binding sites were analyzed by a series of biological experiments (e.g., hemagglutinating and mitogenic activity assays, fluorescence and Circular Dichrosim (CD) spectroscopy) to profoundly identify the role of some key amino acids in the structure-function relationship of PCL.

• Journal of Korean Foot and Ankle Society
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• v.23 no.3
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• pp.91-99
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• 2019

Purpose: This retrospective study reports the intermediate-term clinical outcomes including the practical function in daily and sports activities after total ankle arthroplasty for end-stage rheumatoid arthritis, as well as the effects of modification of perioperative anti-rheumatic medications. Materials and Methods: Twelve patients were followed for a minimum of 2 years after total ankle replacement for end-stage rheumatoid arthritis. Perioperative anti-rheumatic medications in all patients were modified based on a specific guideline. Clinical evaluations consisted of American Orthopaedic Foot and Ankle Society (AOFAS) scores, Foot and Ankle Outcome Score (FAOS), and Foot and Ankle Ability Measure (FAAM) scores. Periodic radiographic evaluation was conducted to detect changes in ankle alignment and postoperative complications. Results: Mean AOFAS, FAOS, and FAAM scores improved significantly from 37.5 to 81.2, 39.1 to 72.4, and 33.8 to 64.0 points at final follow-up, respectively (p<0.001). Functional outcomes in daily and sports activities at final follow-up were found to be 76.5 and 55.8 points for the FAOS and 70.5 and 57.5 points for the FAAM, respectively. As early postoperative complications, there was one case of local wound necrosis, one case of medial malleolar fracture, and one case of deep peroneal nerve injury. Radiological evaluation revealed two cases of asymptomatic heterotopic ossification and one case of progressive arthritis in the talonavicular joint. Reoperation was performed in only one patient (8.3%) with a medial soft tissue impingement at a mean of 35.6 months follow-up. Conclusion: Total ankle arthroplasty appears to be an effective surgical option for end-stage rheumatoid arthritis. Practical functions in daily and sports activities were significantly improved at intermediate-term follow-up. Modification of perioperative anti-rheumatic medications can be one of the solutions to reduce the postoperative complication rate.

• Korean Journal of Metals and Materials
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• v.48 no.9
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• pp.807-812
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• 2010

In this paper, the Ultrasonic Nanocrystalline Surface Modification (UNSM) surface treatment process was used to induce compressive residual stress and nanocrystalline structure by severe plastic deformation on the UNSM-treated surface. The test results for AISI304 stainless steel demonstrated that the grain size was found to be 23 nm, the dislocation density was increased by $0.2085{\times}10^{18}\;m^{-2}$, and the volume fraction of martensite is defined as 27.6% from austenite so that the surface hardness of the surface is increased from 200 Hv up to 515 Hv. The initial tensile residual stress is changed from 300 MPa to a compressive residual stress of 500 MPa after UNSM treatment. In addition, UNSM was applied under five various conditions, and the results of those conditions were defined as a function of depth quantitative.

• Journal of the Korea Society of Computer and Information
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• v.26 no.11
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• pp.101-110
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• 2021

The Weapon Control Software performs the function of supporting weapon operation within the Naval Combat System in connection with the Weapon System. As Weapon Control Software depends on an Weapon System, it has the characteristic that software modification is unavoidable with the change in Interface information. Modification of software causes an increase in development costs since it must take verification step such as software reliability test. In this paper, We design the standard architecture of weapon control software to minimize the modification elements of existing weapon control software. For Interface information management, Feature Model were applied to make a division between common factor and variable factor. In addition, Strategy Pattern were applied to improve the software design. Software evaluation test results show that new architecture provides better modifiability and reuse than existing software as well as the cost of development decrease.

• Molecules and Cells
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• v.45 no.6
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• pp.425-434
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• 2022

The post-translational modification (e.g., phosphorylation) of estrogen receptor α (ERα) plays a role in controlling the expression and subcellular localization of ERα as well as its sensitivity to hormone response. Here, we show that ERα is also modified by UFM1 and this modification (ufmylation) plays a crucial role in promoting the stability and transactivity of ERα, which in turn promotes breast cancer development. The elevation of ufmylation via the knockdown of UFSP2 (the UFM1-deconjugating enzyme in humans) dramatically increases ERα stability by inhibiting ubiquitination. In contrast, ERα stability is decreased by the prevention of ufmylation via the silencing of UBA5 (the UFM1-activating E1 enzyme). Lys171 and Lys180 of ERα were identified as the major UFM1 acceptor sites, and the replacement of both Lys residues by Arg (2KR mutation) markedly reduced ERα stability. Moreover, the 2KR mutation abrogated the 17β-estradiol-induced transactivity of ERα and the expression of its downstream target genes, including pS2, cyclin D1, and c-Myc; this indicates that ERα ufmylation is required for its transactivation function. In addition, the 2KR mutation prevented anchorage-independent colony formation by MCF7 cells. Most notably, the expression of UFM1 and its conjugating machinery (i.e., UBA5, UFC1, UFL1, and UFBP1) were dramatically upregulated in ERα-positive breast cancer cell lines and tissues. Collectively, these findings implicate a critical role attributed to ERα ufmylation in breast cancer development by ameliorating its stability and transactivity.

• Journal of the Korean Institute of Telematics and Electronics
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• v.17 no.1
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• pp.23-30
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• 1980

The reconstruction problem in a low dose case requires some compromise of resolution and noise artifacts, and also some modification of filter kernels depending on the signal-to-noise ratio of projection data. In this paper, ail algorithm for the reconstruction of an image function from noisy projection data is suggested, based on minimum-mean-square error criterion. Modification of the falter kernel is made from information (statistics) obtained from the projection data. The simulation study Proves that this algorithm, based on the Wiener falter approach, provides substantially improved image with reduction of noise as well as improvement of the resolution. An approximate method was also studied which leads to the possible use of a recursive filter in the convolution process of image reconstruction.

• Journal of Environmental Health Sciences
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• v.30 no.4
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• pp.314-319
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• 2004

Glycinin of more than $97\%$ purity was modified using maleic anhydride. Glycinin samples of $0\%,\;65\%,\;and\;95\%$ lysine residue modifications were used to determine the changes in turbidimetric characteristics of the protein due to maleylation. The solubility behavior of the protein as a function of pH was changed with maleylation. The isoelectric point of $65\%\;and\;95\%$ modified glycinin shifted to pH 4.0 and pH 3.5-4.0, respectively, as compared to pH 4.6 for native glycinin. Maleylated glycinins exhibited increased solubility at pH above 4.6. Turbidity of native glycinin decreased substantially by the addition of NaCl, but the stabilizing effect of NaCl decreased when the protein was chemically modified. The effect of NaCl on $65\%$ modified glycinin was intermediate between native glycinin and $95\%$ modified sample. Thermal aggregation of native glycinin was completed within 5 min of heating at $80^{\circ}C$. Maleylation contributed significantly to the thermostability of the protein at pH of 7.0 and 9.0, exhibiting little turbidity. Addition of NaCl suppressed thermal aggregation of native glycinin, but turbidity actually increased for the samples of $65\%\;and\;95\%$ modification.

• 제어로봇시스템학회:학술대회논문집
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• 1997.10a
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• pp.179-182
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• 1997

In a training simulator for power plant, operator's action in the MCR(Main Control Room) are given to plant process and computer system model as an inputs, and the same response as in real power plant is provided in real time. Inter-process communication and synchronization are especially important among various inputs. In the plant simulator, to simulate the digital control system such as FOXBORO SPEC-200 Micro control system, modification and adaptation of control card(CCC) and its continuous display station(CDS) is necessary. This paper describes the modeling and simulation of FOXBORO SPEC-200 Micro control system applied to Younggwang nuclear power plant unit #3 & 4, and its integration process to the full-scope replica type training simulator. In a simulator, display station like CDS of FOXBORO SPEC-200 Micro control system is classified as ITI(Intelligent Type Instrument), which has a micro processor inside to process information and the corresponding alphanumeric display, and the stimulation of ITI limits the important functions in a training simulator such as backtrack, replay, freeze and IC reset. Therefore, to achieve the better performance of the simulator, modification of CDS and special firmware is developed to simulate the FOXBORO SPEC-200 Micro control system. Each control function inside control card is modeled and simulated in generic approach to accept the plant data and control parameter conveniently, and debugging algorithms are applied for massive coding developed in short period.

• Plant Journal
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• v.16 no.3
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• pp.37-41
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• 2020

Lately old LNG carriers increased and ship price is getting down. So Interest for reuse and modification of used LNG carriers is growing. Also the needs for replacement of old power plant is increasing. Additionally eco friendly fuel such as LNG become attractive. Consequently gas power plant is getting much more popular than before. So in this research planning, we consider the floating power plant by modifying LNG carriers. This plant has the various function including storage, power plant and bunkering fuction etc. Through this multifunctional plant, we are ready for the old power plant shutdown and energy crisis in the future when we can supply the urgent mobile floating power plant quickly in time.

• Journal of Information Technology Services
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• v.14 no.2
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• pp.129-142
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• 2015

The purpose of this study is to suggest an effective regression testing method in order to minimize the scope of test resulting from the modification of software and to prevent mismatch of test case and test objects. As a way to improve the efficiency of regression testing which uses a change-centric testing technique, the method flow is analyzed and grasped through a static analysis based on source code in order to identify modified parts. After the order of priority is set according to the results of user action log-based dynamic analysis on identified regression testing objects, test effect can be raised by adjusting the order of priority using code complexity. Quality assurance coverage can be checked using the user action log suggested in this study, and the progress of test and whether or not each function has been verified can be checked, too. In addition, by minimizing test parts and adjusting the order of test, costs and time can be saved, making it possible to conduct regression testing effectively.