• Applied Microscopy
• /
• v.51
• /
• pp.17.1-17.10
• /
• 2021

Our previous study on the binding activity between Cel5H and clay minerals showed highest binding efficiency among other cellulase enzymes cloned. Here, based on previous studies, we hypothesized that the positive amino acids on the surface of Cel5H protein may play an important role in binding to clay surfaces. To examine this, protein sequences of Bacillus licheniformis Cel5H (BlCel5H) and Paenibacillus polymyxa Cel5A (PpCel5A) were analyzed and then selected amino acids were mutated. These mutated proteins were investigated for binding activity and force measurement via atomic force microscopy (AFM). A total of seven amino acids which are only present in BlCel5H but not in PpCel5A were selected for mutational studies and the positive residues which are present in both were omitted. Of the seven selected surface lysine residues, only three mutants K196A(M2), K54A(M3) and K157T(M4) showed 12%, 7% and 8% less clay mineral binding ability, respectively compared with wild-type. The probable reason why other mutants did not show altered binding efficiency might be due to relative location of amino acids on the protein surface. Meanwhile, measurement of adhesion forces on mica sheets showed a well-defined maximum at 69±19 pN for wild-type, 58±19 pN for M2, 53±19 pN for M3, and 49±19 pN for M4 proteins. Hence, our results demonstrated that relative location of surface amino acids of Cel5H protein especially positive charged amino acids are important in the process of clay mineral-protein binding interaction through electrostatic exchange of charges.

• Journal of KIISE:Software and Applications
• /
• v.37 no.8
• /
• pp.611-619
• /
• 2010

This paper presents an efficient modeling system of virtual pottery in which the user can deform a body of virtual clay with a haptic tool for E-learning. We propose a Circular Sector Element Method (CSEM) which represents the virtual pottery with a set of circular sector elements based on the cylindrical symmetry of pottery. Efficient algorithms for collision detection and response, interactions between adjacent elements, and GPU-based visual-haptic synchronization are designed and implemented for the CSEM. Empirical evaluation showed that the modeling system is computationally efficient with finer details and provides convincing model deformation and force feedback. The developed system, if combined with educational contents, is expected to be used as an effective E-learning platform for elementary school students.

• Journal of the Korean Geosynthetics Society
• /
• v.6 no.1
• /
• pp.17-25
• /
• 2007

This study presents a modeling approach for geogrid-encased stone column(GESC) method which is widely used in Europe as an alternative to conventional pile foundations. Several benefits of using the stone column method include sound performance, low cost, expediency of construction, and liquefaction resistance, among others. Recently, geosynthetic-encased stone column approach has been developed to improve load carrying capacity through increasing confinement effect. The aim of this research is to establish a systematic approach for modeling of GESC and to form a database for the fundamentals of GESC. This paper presents details of 3D modeling of GESC together with the general behavior of GESC.

• Journal of Industrial Technology
• /
• v.27 no.A
• /
• pp.111-120
• /
• 2007

This paper is results of experimental research on the effect of application of similarity related to permeability of soil on the consolidation behavior as centrifuge modeling of consolidation is performed with the centrifuge model facility. In this research, the permeability of soil was controlled by changing the viscosity of porewater as the mixed water with glycerin was used during the centrifuge model experiments. The effect of drainage path on consolidation was investigated by installing the vertical drains. A serise of centrifuge model tests with conditions of single vertical and radial horizontal drainage were carried out. Kaolinite and Jumunjin standard sand were used as soft clay and surcharges respectively during tests. For testing condition of single vertical drainage considering similarity of permeability, it was found that consolidation with mixed porewater with glycerin was delayed in comparisons sons with test results with water only. For conditions of horizontal drainage with vertical drains, a low permeability by changing the viscosity of pore water resulted in delayed degree of consolidation at an initial stage of consolidation. But, it predicted not much differences in settlement as long as the consolidation time was sufficiently long enough to finish consolidation. Consequently, it was found that similarity in permeability should be considered to be critical for the case of centrifuge model experiments related to consolidation with long drainage path.

• Geomechanics and Engineering
• /
• v.30 no.6
• /
• pp.539-549
• /
• 2022

Accidental anchor drop can cause disturbances to seabed materials and pose significant threats to the safety and serviceability of submarine structures such as pipelines. In this study, a series of anchor drop tests was carried out to investigate the penetration mechanism of a Hall anchor in sand and clay. A special anchor drop apparatus was designed to model the inflight drop of a Hall anchor. Results indicate that Coriolis acceleration was the primary cause of large horizontal offsets in sand, and earth gravity had negligible impact on the lateral movement of dropped anchors. The indued final horizontal offset was shown to increase with the elevated drop height of an anchor, and the existence of water can slow down the landing velocity of an anchor. It is also observed that water conditions had a significant effect on the influence zone caused by anchors. The vertical influence depth was over 5 m, and the influence radius was more than 3 m if the anchor had a drop height of 25 m in dry sand. In comparison, the vertical influence depth and radius reduced to less than 3 m and 2 m, respectively, when the anchor was released from 10 m height and fell into the seabed with a water depth of 15 m. It is also found that the dynamically penetrating anchors could significantly influence the earth pressure in clay. There is a non-linear increase in the measured penetration depth with kinematic energy, and the resulted maximum earth pressure increased dramatically with an increase in kinematic energy. Results from centrifuge model tests in this study provide useful insights into the penetration mechanism of a dropped anchor, which provides valuable data for design and planning of future submarine structures.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.9 no.6
• /
• pp.1548-1554
• /
• 2008

The market of silver precious metal clay (PMC) is promising as its application for agile metal craft. The property of suitable hardness and shrinkage as well as environment-safe is strictly required as people make the final product with bare hands. We propose a silver PMC with new organic binder made of palm oil, glycolic acid and water. Then we prepared disk specimen of our proposed PMC and well-known commercial PMC. We investigated the hardness, weight change, linear shrinkage, density and micro structure evolution with sintering temperature of $700{\sim}900^{\circ}C$ ($50^{\circ}C$ gap) for 15minutes. We confirm our proposed PMC has suitable property for craft product comparable for commercial PMC, and the optimum sintering condition is $850^{\circ}C$-15minutes for metal craft application.

• The Journal of Engineering Geology
• /
• v.31 no.2
• /
• pp.187-197
• /
• 2021

Clay mineral content of weathered zone is a key parameter for landslide studies. Electrical resistivity tomography is usually performed to delineate the geometry of complex landslides and to identify the sliding surface. In clay-bearing weathered zone, parallel resistivity Archie equation is employed to investigate the effect of conductivity added (resistivity reduced) by clay minerals of kaolinite and montmorillonite, which is dependent on their specific surface area and cation exchange capacities (CEC). A decrease of overall resistivity and apparent formation factor is observed with increasing pore-water resistivity, significantly in montmorillonite. Formation factor is found decreased with increasing porosity and decreasing cementation factor. Parallel Archie equation was applied to the electrical resistivity data from the test area (Sinjindo-ri, Taean-gun, Chungcheongnam-do, Korea) which experienced land creeping in the year of 2014. A panel test with varying clay-mineral contents provides the best fit section when the theoretical section constructed with the assumed contents approaches the field section, from which the clay-mineral content of the weathered zone is estimated to be approximately 10%. Resistivity interpretation schemes including the clay mineral contents for land creeping studies explored in this paper can be challenged more when porosity, saturation, and pore-water resistivity are provided and they are included in the numerical resistivity modeling.

• Geomechanics and Engineering
• /
• v.1 no.4
• /
• pp.307-321
• /
• 2009

The paper employs a feed forward neural network with back-propagation algorithm for modeling time dependent swell in clays containing carbonate in the presence of sulfuric acid. The oedometer swell percent is estimated at a nominal surcharge pressure of 6.25 kPa to develop 612 data sets for modeling. The input parameters used in the network include time, sulfuric acid concentration, carbonate percentage, and liquid limit. Among the total data sets, 280 (46%) were assigned to training, 175 (29%) for testing and the remaining 157 data sets (25%) were relegated to cross validation. The network was programmed to process this information and predict the percent swell at any time, knowing the variable involved. The study demonstrates that it is possible to develop a general BPNN model that can predict time dependent swell with relatively high accuracy with observed data ($R^2$=0.9986). The obtained results are also compared with generated non-linear regression model.

• Structural Engineering and Mechanics
• /
• v.33 no.1
• /
• pp.67-77
• /
• 2009

The soil response calculation is described, by which, threw the fictive path of stress, the stress-deformation diagrams are determined, considering the nonlinear soil behavior. The calculation are lead incrementally, by which is shown that in the presented soil model (modified Cam Clay), considering the influence of overconsolidated soil pressure OCR, the number of calculation steps may, but not necessarily, have a sufficient influence on the value of failure load and definite soil deformation. The simplicity and the practicalness of the procedure, the enables modeling the complex relations in soil.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.7 no.3
• /
• pp.409-413
• /
• 2006

The accumulated deformation due to the undrained creep causes the general stability problem for the overall soil mass. In this study, the time-dependent constitutive equation, into which a damage law, modified cam clay model, and Perzyna's generalized viscous theory were incorporated, was derived microscopically. The model prediction agreed well with the experimental result including the case of the undrained creep rupture.