• Bulletin of the Korean Chemical Society
• /
• v.34 no.8
• /
• pp.2425-2430
• /
• 2013

A simple, fast and robust analytical method was developed to determine imatinib in human plasma using liquid chromatography-tandem mass spectrometry with electrospray ionization in the positive ion mode. Imatinib and labeled internal standard were extracted from plasma with a simple protein precipitation. The chromatographic separation was performed using an isocratic elution of mobile phase involving 5.0 mM ammonium formate in water-5.0 mM ammonium formate in methanol (30:70, v/v) over 3.0 min on reversed-stationary phase. The detection was performed using a triple-quadrupole tandem mass spectrometer in multiple-reaction monitoring mode. The developed method was validated with lower limit of quantification of 10 ng/mL. The calibration curve was linear over 10-2000 ng/mL ($R^2$ > 0.99). The method validation parameters met the acceptance criteria. The spiked samples and standard solutions were stable under conditions for storage and handling. The reliable method was successfully applied to real sample analyses and thus a pharmacokinetic study in 27 healthy Korean male volunteers.

• Bulletin of the Korean Chemical Society
• /
• v.25 no.1
• /
• pp.109-114
• /
• 2004

A sensitive method for quantitation of glimepiride in human plasma has been established using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI/MS/MS). Glipizide was used as an internal standard. Glimepiride and internal standard in plasma sample was extracted using diethyl etherethyl acetate (1 : 1). A centrifuged upper layer was then evaporated and reconstituted with the mobile phase of acetonitrile-5 mM ammonium acetate (60:40, pH 3.0). The reconstituted samples were injected into a $C_{18}$ reversed-phase column. Using MS/MS in the multiple reaction monitoring (MRM) mode, glimepiride and glipizide were detected without severe interference from human plasma matrix. Glimepiride produced a protonated precursor ion ([M+H]$^+$) at m/z 491 and a corresponding product ion at m/z 352. And the internal standard produced a protonated precursor ion ([M+H]]$^+$) at m/z 446 and a corresponding product ion at m/z 321. Detection of glimepiride in human plasma by the LC-ESI/MS/MS method was accurate and precise with a quantitation limit of 0.1 ng/mL. The validation, reproducibility, stability, and recovery of the method were evaluated. The method has been successfully applied to pharmacokinetic studies of glimepiride in human plasma.

• The Korean Journal of Mycology
• /
• v.37 no.2
• /
• pp.161-166
• /
• 2009

The purpose of the present study is to develop a simple, fast and sensitive LC/MS method for simultaneous separation and the determination of an active component in the oriental medicinal mushroom Cordyceps militaris. Based on this work, the contents of cordycepin in Cordyceps militaris fruiting cultivated on various media were determined and compared. And also, the nutritional components such as minerals and vitamins were determined in order to provide useful information to consumer as a food material. The analysis methods of nutritional components were chosen on the basis of AOAC. The optimum separation for cordycepin was achieved using a solvent gradient consisting of the mixture of 0.1% formic acid in methanol (solvent B) in a background of 0.1% formic acid in water (solvent A) as a mobile phase and a 3.0${\times}$150 Waters XTera column. Selective ion monitoring (SIR) mode ([M+H]+ at m/z 252) was used for quantitative analysis of cordycepin. The cultivated Cordyceps militaris on various media contained 1~14 /g of cordycepin, 0.65~1.08% of thiamine, 0.86~7.17% of riboflavin, and 3.01~5.26% of niacin. The content of mineral components varied on categories, especially contained 500~3500% of potassium as a major mineral. Cordycepin, niacin and potassium were found much higher in the fruiting cultivated with soy power media (gold 10) than other media.

• Journal of the Korea Convergence Society
• /
• v.11 no.3
• /
• pp.85-93
• /
• 2020

This study is to examine the randomized controlled trials abroad studies on ICT-based convergence studies in the elderly, and to investigate the types and effects of ICT-based interventions. Eight studies that meet the selection criteria were selected from 326 studies identified by three databases and hand-searching. As a result of this study, the most commonly used ICT were the three internet, followed by two tablets and mobile phones, one twoway videoconferencing, one Kinect, one robot, and one Blood Glucose Monitoring System. These results could be used for ICT-based convergence studies in Korea, and further studies should be conducted with higher quality studies such as randomized controlled trials.

• KSBB Journal
• /
• v.25 no.5
• /
• pp.449-452
• /
• 2010

L-Thyroxine possessing a chiral center, the naturally occurring thyroid hormone has been used for the treatment of thyroid dysfunctions and marketed as levothyroxine (L-thyroxine) sodium salt. In this study, after extraction of levothyroxine tablet as a pre-treatment process, direct enantiomer separation of thyroxine on crown ether derived chiral columns for determination of optical purity was performed using reversed mobile phase with acid additive. The chromatographic method developed in this study was applied in the determination of optical purity of several current domestic and foreign commercialized levothyroxine tablets. Optical purity values of these commercialized L-thyroxine sodium tablets except one were higher than 99 percents.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.22 no.4
• /
• pp.587-594
• /
• 2018

The purpose of this study is to propose a power demand volatility evaluation system based on LSTM and not to verify the accuracy of the demand module which is a core module, but to recognize the sudden change of power pattern by using deeplearning in the actual power demand monitoring system. Then we confirm the availability of the module. Also, we tried to provide a visualized report so that the manager can determine the fluctuation of the power usage patten by applying it as a module to the web based system. It is confirmed that the power consumption data shows a certain pattern in the case of government offices and hospitals as a result of implementation of the volatility evaluation system. On the other hand, in areas with relatively low power consumption, such as residential facilities, it was not appropriate to evaluate the volatility.

• Journal of Pharmaceutical Investigation
• /
• v.34 no.6
• /
• pp.513-519
• /
• 2004

A sensitive method for quantification of pinaverium bromide in human plasma was established using liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI-MS/MS). Glimepiride was used as internal standard. Pinaverium bromide and internal standard in plasma sample were extracted using tert-butylmethylether(TBME). A centrifuged upper layer was then evaporated and reconstituted with mobile phase of acetonitrile-5 mM ammonium formate (80/20, pH 3.0). The reconstituted samples were injected into a $C_{18}$ reversed-phase column. Using MS/MS with multiple reaction monitoring (MRM) mode, pinaverium and glimepirde were detected without severe interference from human plasma matrix. Pinaverium produced a protonated precursor ion $([M+H]^+)$ at m/z 510.3 and a corresponding product ion at m/z 228.9. Internal standard produced a protonated precursor ion $([M+H]^+)$ at m/z 491.5 and a corresponding product ion at m/z 352.0. Detection of pinaverium bromide in human plasma was accurate and precise, with limit of quantitation at 0.5 ng/ml. The method has been successfully applied to bioavailability study of pinaverium bromide tablet in Korean healthy male volunteers. Pharmacokinetic parameters such as $AUC_t,\;C_{max},\;T_{max},\;K_{el}\;and\;t_{1/2}$ were calculated.

• Journal of the Korea Institute of Information Security & Cryptology
• /
• v.26 no.5
• /
• pp.1105-1119
• /
• 2016

Android includes SEAndroid as a core security feature. SELinux is applied to Android OS as a SEAndroid, because there exists structural differences between Linux and Android. Since the security of SEAndroid depends on the reliable policy if the policy is tampered by the attacker, the serious security problems can be occurred. So we must protect policies which are the most important thing in SEAndoroid. In this paper, we analyze the process of SEAndroid policy updating to find out vulnerabilities and study the attack points on policy tampering. And we propose the SPPA to detect whether the policy is modified by an attacker. Moreover, we prove the performance and the effect of our proposed method on mobile device.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2017.10a
• /
• pp.415-418
• /
• 2017

Recently, the demand for energy efficiency improvement technology through the connection of waste heat energy and SmartGrid has been increasing. Thus, investments for the cultivation of high value crops and produce is increasing through research aimed at synthetic technology in real-time utilization of smart farms and waste heat energy with the concept of using container greenhouses and plant factories. In this aspect, we have carried out research on a practical application technology that will help farmers to increase the economic effectiveness of LED based plant factories in terms of energy efficiency. This can provide opportunities to connect with the large scale automated smart farms in the future. In this study, we focused on the economic effectiveness of crop cultivation using cooling technology in a container greenhouse through waste heat energy. Hereafter, in order to further advance the technology of real-time monitoring/control of the absorption chiller which is used through the container greenhouses and waste heat energy by using IOT, we would like to propose research on new ideas of agricultural technology that can maximize the utility of cooling energy by operating a mobile gateway based on Raspberry PI.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.16 no.9
• /
• pp.6260-6268
• /
• 2015

In LTE-Advanced that different networks coexist, it is considered that it is actually difficult to provide service continuity with a procedural and static control method applied to the existing voice service. This paper suggests Service Self-Organization to support the service continuity effectively based on SON. It means a method in which a subscriber's terminal collects information about its current condition and base station around, and a base station, through the data collected by monitoring inner or adjacent base station, shares related data and converges, controlling service continuity on its own. In other words, as context information of mobile terminal and base station changes, set-up of related functions such as ISHO, cell selection, source allocation, load control, and QoS mapping is adapted; each function fits into the change, exchanges the process of reorganization, and interacts; these actions go toward to satisfy service continuity. Simulation results show that it provides better performances than the conventional one with the measure of resource utilization rate and outage probability.