• Asian-Australasian Journal of Animal Sciences
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• v.28 no.10
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• pp.1454-1464
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• 2015

Four methods were adopted, including the mobile nylon bag (MNB) method, modified three-step in vitro (MTS) method, original three-step in vitro (OTS) method, and acid detergent insoluble nitrogen (ADIN) estimating method, to evaluate the intestinal digestibility of rumen undegradable protein (DRUP) of 10 types of concentrates and 7 types of roughages. After correlation analysis to determine the DRUP values using the MNB, MTS, OTS, and ADIN methods, the study aimed to find out appropriate methods to replace the MNB method due to its disadvantages such as high price, long time period, and use of a duodenal T-fistula. Three dairy cows with a permanent ruminal fistula and duodenal T-fistula were used in a single-factor experimental design. The results showed that the determined DRUP values using the MNB method for soybean meal, cottonseed meal, rapeseed meal, sunflower meal, corn germ meal, corn, rice bran, barley, wheat bran, corn fiber feed, Alfalfa (Zhao dong), Alfalfa (Long mu 801), Alfalfa (Long mu 803), grass (North), Grass (Inner Mongolia), corn silage and corn straw were 98.13%, 87.37%, 88.47%, 82.60%, 75.40%, 93.23%, 69.27%, 91.27%, 72.37%, 79.03%, 66.72%, 68.64%, 73.57%, 50.47%, 51.52%, 54.05%, and 43.84%, respectively. The coefficient of determination ($R^2=0.964$) of the results between the MTS method and the MNB method was higher than that ($R^2=0.942$) between the OTS method and the MNB method. The coefficient of determination of the DRUP values of the concentrates among the in vitro method (including the MTS and OTS methods) and the MNB method was higher than that of the roughage. There was a weak correlation between the determined DRUP values in concentrates obtained from the ADIN method and those from the MNB method, and there was a significant correlation (p<0.01) between the determined DRUP values of the roughage obtained from the MNB method and those obtained from ADIN method. The DRUP values were significantly correlated with the nutritional ingredients of the feeds. The regression equation was DRUP =100.5566+0.4169CP - 0.4344SP - 0.7102NDF - 0.7950EE ($R^2=0.8668$, p<0.01; CP, crude protein; SP, soluble protein; NDF, neutral detergent fiber; EE, ether extract). It was concluded that both the MTS method and the OTS may suitable to replace the MNB method for determining the DRUP values and the former method was more effective. Only the ADIN method could be used to predict the values of the roughages but conventional nutritional ingredients were available for all of the samples' DRUP.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.2
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• pp.113-118
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• 1995

Eighteen Holstein Friesian ${\times}$ Native heifers with an average live weight of $175.4{\pm}27.8kg$ were allotted to 3 dietary groups. All animals were kept individually in a stanchion barn with free access to water and urea-treated rice straw (UTS). The supplemented feeds were as follows: Group (Gr) 1 - concentrate mixture (15% CP) at 1% body weight (BW), Gr 2 - concentrate mixture at 0.7% BW + free licking of multinutrient block (MNB), Gr 3 - as Gr 2 + 0.3% BW ground com. Multinutrient block composed of 20% mineral mixture, 10% urea, 18% molasses, 20% soybean meal, 22% sesame meal, 10% cement, 0.15 million IU and 50 IU/kg MNB of vitamin A and E. The experimental period lasted 12 weeks. The result revealed that MNB enhanced intake of UTS and total dry matter intake. Animals in Gr 3 consumed a higher amount of feed than the other 2 groups, thus resulting in the highest live weight gain and tended to possess the best feed conversion ratio. Heifers in Gr 2 also performed better than Gr 1 although the difference was not significant. Average MNB intake found to be 0.65 kg/day. Feed cost per kg gain was not significantly different amon groups. However if the cost of MNB could be lower, it would benefit the production cost of animals. The advantage of MNB is the safety of using a high level of urea. Although it was supplemented to UTS which also contained non-protein nitrogen (NPN), no toxic sign was shown. The results indicated that MNB could be partially substituted to concentrate mixture and it was even better when used incombination with soluble carbohydrate feed.

• 한국정보디스플레이학회:학술대회논문집
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• 2006.08a
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• pp.1174-1177
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• 2006

A bottom contact organic thin film transistor (OTFT) is fabricated with an organic double-layered gate insulator (GI) and pentacene. The PMMA and MNB layers are treated on gate insulator and source/drain (S/D, Au) before depositing pentacene to investigate device properties and pentacene growth. The sequence of surface treatment affects a device performance seriously. The ultra-thin PMMA (below 50A) was deposited on organic gate insulator and S/D metal by spin coating method, which showed no deterioration of on-state current (Ion) although bottom contact structure was exploited. We proposed that the reason of no contact resistance (Rc) increase may be due to a wettability difference in between PMMA / Au and PMMA / organic GI. As a result, the device treated by $PMMA\;{\rightarrow}\;MNB$ showed much better Ion behavior than those fabricated by $MNB\;{\rightarrow}\;PMMA$. We will report the important physical and electrical performance difference associated with surface treatment sequence.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.3
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• pp.313-316
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• 2000

Three Holstein steers, fitted with ruminal and duodenal cannulas, were used in a replicated $3{\times}3$ Latin square design to determine the digestibility of dietary nitrogen in total digestive tract by three methods, 1) mobile nylon bag (MNB); 2) total fecal collection (TFC); and 3) washed fecal sample after freezing and thawing through a sieve with a pore size of $45{\mu}m$ (WFS). A basal diet of oaten hay-barley was supplemented with one of the following protein sources; soybean meal, fish meal or blood meal. Steers were fed at a level of 2% of body weight. The experimental diets were contained approximately 1.85% nitrogen. There were no differences (p>0.05) among the diets on DM, NDF and nitrogen disappearances, and the diet results were pooled to assess the methods. Total tract disappearances of dry matter and neutral detergent fiber were 61.6, 71.1 and 78.9 and 25.3, 63.2 and 64.6 for MNB, TFC and WFS methods, respectively. The lower digestibility of DM and NDF in the MNB method could be a result of low ruminal incubation time. The TFC method had the lower (p<0.05) determination of nitrogen disappearance in the total digestive tract than the MNB and WFS methods. On the other hand, nitrogen disappearance in the total digestive tract determined by the WFS technique was comparable to that in MNB technique, as there was no significant difference (p>0.05) between the methods. It is shown that the disappearance of dietary nitrogen in the total digestive tract could be estimated in the intact animals by using washed fecal sample prior to freezing and thawing.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.671-674
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• 2002

This study investigated the use of multinutrient blocks (MNB) composed of 35% date syrup, 35% date syrup byproducts, 10% date fronds, 7% urea, 7% cement and 6% common salt for growing small ruminants, as partial substitute for the roughage component of the diet (Rhodes grass hay) and its effect on feedlot performance and economics of feeding. Eight growing local Omani goats and 8 sheep (each about one year old) were used in this study. Mean body weights for goats and sheep (kg), respectively were $21.1{\pm}4.5$ and $25.5{\pm}4.1$. The goats and sheep were subdivided into two sub-groups of more or less equal body weights for each species. Each sub-group in both species was either fed on 0.5 kg concentrate+ad libitum Rhodes grass hay or the same diet+restricted hay (about 0.2 kg/head/day) and ad libitum amount of MNB. Sheep significantly (p<0.05) consumed greater amounts of MNB ($36{\pm}17$g/head/day) than goats ($6{\pm}2.5$ g/head/day). Feeding of the MNB was effective in sparing about 40% of the roughage Rhodes grass hay for goats (from 240 to 140 g) and about 42% for sheep (from 252 to 146 g) or approximately 100 g/head/day for both goats and sheep. This reduction (or sparing) in the consumption of Rhodes grass hay was coupled by an improvement in daily liveweight gain (g/head/day) in both goats (from 29 to 46 by 58.6%) and sheep (from 26 to 39 by 50%) and also by an improvement in the feed conversion efficiency (g feed/g gain) of both goats (from 25 to 13.8 by 45%) and sheep (from 28.7 to 17.2 by 40%). Cost of daily consumed feeds as well as cost/kg gain (or cost of meat) were both reduced due to feeding of MNB. They were both respectively reduced by 7.5% (from 53 Baisa/day to 49) and 38% (from 1,828 Baisa/kg to 1,140). It was economically viable to feed MNBs containing date by-products and urea to small ruminants in the Sultanate of Oman.

• Journal of Environmental Science International
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• v.20 no.3
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• pp.291-299
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• 2011

The main objectives of this research are to investigate characteristics of ozone solubility due to low solubility of conventional bubbles-ozone generators, evaluate the treatment characteristics of reclaiming textile wastewater for industrial water by means of micro/nano bubbles-dissolved ozone flotation(MNB-DOF) process. The textile wastewater used in this research was obtained from final effluent of the textile wastewater in B city. There is a 400L reactor which consists of a micro-nano bubble system and a ozone generator for experiments. As a result of generating micro-nano bubbles (below $0.5{\mu}m$) by using of MNB-DOF process, it improved ozone solubility due to higher ozone transfer rates. Consequently, the shorter ozonation time clearly indicates the lower power costs. The reported results clearly indicated that MNB-DOF process can be effectively and inexpensively. Results of the experiments through MNB-DOF process in this study satisfy all reclaiming standards as industrial water: pH 6.5~8.5, SS 10 mg/L or below, $BOD_5$) 6 mg/L or below, turbidity 10 NTU or below, Coliforms 1,000/100 mL or below. Therefore there is a possibility of the reclaiming of the textile wastewater as industrial water.

• Korean Journal of Child Studies
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• v.13 no.2
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• pp.99-112
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• 1992

The purpose of the present study was to investigate methodological and theoretical problems in measuring the acquisition of national identity of in-country and over-seas Korean adolescents. The subjects consisted of 344 in-country and 89 over-seas (total 433) adolescents. 14 to 26 years of age. Data were collected with the use of two questionnaires. The Cognition of National Characteristics(CNC) and The Measure of National Belongings(MNB). As statistical measures. F-test and Pearson's correlation coefficient were used. The results are disclosed as follows; 1) The concept of national identity organized not only cognition of the national characteristics in multi-dimension which is related each other, but also a sense of belonging. 2) Two measuring methods(CNC and MNB) for this study are closely related. 3) Overseas Korean adolescents performed more than in-country Korean adolescents in 5 sub-national characteristics(language, social norm, history of the nation, & breaking with ideological thinking) except the consanguinity, of CNC and MNB. Crisis hypothesis was supposed as alternative factor to explain that overseas Korean adolescents cognified about the national characteristics and feel a sense of belonging more than the in-country respondents. 4) Some demographic variables, such as age of the respondents, place of birth, level of understanding Korean language, are contributed to acquiring national identity.

• Plant Resources
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• v.4 no.1
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• pp.36-40
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• 2001

Transgenic Petunia hybrida cv. Rosanpion was produced by Agrobactepium tumefaciens LBA4404 harboring a binary vector pBI 121 containing $\beta$-glucuronidase (gus) and neomycin phosphotransferase (nptII). For genetic transformation, leaf discs were precultured on MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA (MNB) for 2 days and cocultured for 15 mins with A. tumefaciens. For selection of transformant, leaf discs were transferred to fresh MNB containing 50 mg/L kanamycin and 500 mg/L cefotaxime. Eighteen plants were regenerated and four were confirmed by PCR for detection of gus and nptII gene integrated into the nuclear genome of petunia ‘Rosanpion’. Using this transformation system, we expect that transgenic petunia ‘Rosanpion’ incorporating a useful gene can be produced.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.6
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• pp.781-787
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• 2009

The sense and antisense digoxigenin-labeled RNA probes of four genes, Cdc25A, Cdc25B, Sox2 and Mnb, were produced by using SP6 and T7 RNA polymerases, respectively, and in vitro transcription. Expression patterns of the four genes were detected by in situ hybridization in HH (Hamburger and Hamilton) stage 10 chick embryos. In general, expression patterns of the four genes were similar. mRNA of the four genes was mostly restricted to the entire CNS (central nervous system). All were confined to an identical region, neural tube, neural groove and caudal neural plate, corresponding to the notochord or spinal cord, but there was some distinction in specific region or in concentration, for example in somites. The overlap in expression at the same developmental stage in the CNS suggests that the four genes may be functional similar or related in CNS development. Expression patterns of the four genes support specific roles of these regulators in the developing CNS.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.11 no.6
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• pp.2996-3011
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• 2017

With rapid growth of web technology and dissemination of smart devices, social networking service(SNS) is widely used. As a result, huge amount of data are generated from SNS such as Twitter, and sentiment analysis of SNS data is very important for various applications and services. In the existing sentiment analysis based on the $Na{\ddot{i}}ve$ Bayes algorithm, a same number of attributes is usually employed to estimate the weight of each class. Moreover, uncountable and meaningless attributes are included. This results in decreased accuracy of sentiment analysis. In this paper two methods are proposed to resolve these issues, which reflect the difference of the number of positive words and negative words in calculating the weights, and eliminate insignificant words in the feature selection step using Multinomial $Na{\ddot{i}}ve$ Bayes(MNB) algorithm. Performance comparison demonstrates that the proposed scheme significantly increases the accuracy compared to the existing Multivariate Bernoulli $Na{\ddot{i}}ve$ Bayes(BNB) algorithm and MNB scheme.