Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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The Expression Patterns of Cdc25A, Cdc25B, Sox2 and Mnb in Central Nervous System in Early Chicken Embryos

  • Zhang, Hui (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Qin, Junhui (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Cao, Jingjing (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Hei, Nainan (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Xu, Chunsheng (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Yang, Ping (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Liu, Haili (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Chu, Xiaohong (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Bao, Huijun (College of Veterinary Medicine, Nanjing Agricultural University) ;
  • Chen, Qiusheng (College of Veterinary Medicine, Nanjing Agricultural University)
  • Received : 2008.09.12
  • Accepted : 2009.01.19
  • Published : 2009.06.01
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Abstract

The sense and antisense digoxigenin-labeled RNA probes of four genes, Cdc25A, Cdc25B, Sox2 and Mnb, were produced by using SP6 and T7 RNA polymerases, respectively, and in vitro transcription. Expression patterns of the four genes were detected by in situ hybridization in HH (Hamburger and Hamilton) stage 10 chick embryos. In general, expression patterns of the four genes were similar. mRNA of the four genes was mostly restricted to the entire CNS (central nervous system). All were confined to an identical region, neural tube, neural groove and caudal neural plate, corresponding to the notochord or spinal cord, but there was some distinction in specific region or in concentration, for example in somites. The overlap in expression at the same developmental stage in the CNS suggests that the four genes may be functional similar or related in CNS development. Expression patterns of the four genes support specific roles of these regulators in the developing CNS.

Keywords

References

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