• KSBB Journal
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• v.30 no.6
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• pp.307-312
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• 2015

Transgenic rice cells using RAmy3D promoter can provide high productivity, and the production of recombinant protein is induced by sugar starvation. In this system, productivity was reduced during the scale-up processes. To ensure the influences of shear stress and oxygen transfer rate, working volume and mixing performances were investigated under various agitation speeds and working volumes. In addition, inoculation methods including suspended cells and filtered cells were compared. Working volumes and shaking speeds were 300, 450 mL and 80, 120 rpm, respectively. Hydrodynamic environment of each condition was measured numerically like mixing time and $k_La$. Good mixing performance and high shear stress were measured at high agitation speed and low volume. The highest level of hCTLA4Ig was 30.7 mg/L at 120 rpm, 300 mL. When conditioned medium was used for inoculation, increased cell growth was noticed during the day 0~4 and decreased slower than filtered cells. Compared with filtered cells, the maximum hCTLA4Ig level reached 37.8 mg/L at 120 rpm, 300 mL and lower protease activity level was observed. In conclusion mixing performance is critical factor for productivity and conditioned medium can have a positive effect on damaged cells caused by hydrodynamic shear stress.

• Korean Journal of Metals and Materials
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• v.48 no.7
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• pp.630-638
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• 2010

The present investigation has been performed on the mixing behavior and microstructural development during fabrication of Fe micro-nano powder feedstock for a micro-powder injection molding process. The mixing experiment using a screw type blender system was conducted to measure the variations of torque and temperature during mixing of Fe powder-binder feedstock with progressive powder loading for various nano-powder compositions up to 25%. It was found that the torque and the temperature required in the mixing of feedstock increased proportionally with increasing cumulative powder loading. Such an increment was larger in the feedstock containing higher content of nano-powder at the same powder loading condition. However, the maximum value was obtained at the nano-powder composition of not 25% but 10%. It was owing to the 'roller bearing effect' of agglomerate type nano-powder acting as lubricant during mixing, consequently leading to the rearrangement of micro-nano powder in the feedstock. It is concluded that the improvement of packing density by rearrangement of nano-powders into interstices of micro-powders is responsible for the maximum powder loading of about 71 vol.% in the nano-powder composition of 25%.

• Journal of Advanced Marine Engineering and Technology
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• v.29 no.7
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• pp.736-743
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• 2005

In this study, three different types of mechanical ventilation systems are compared based on their ventilation characteristics: tracer gas concentration decay characteristics, and ventilation effectiveness by calculating actual ventilation air flow rate. The experiments are performed by using a step-down method for measuring tracer gas. $CO_{2}$ gas, concentration in the model chamber. Application of a mixing factor, k, was used and measured values ranged from 0.68 to 0.77. The Type 2 ventilation system was found to have the highest ventilation effectiveness rather than the Types 1 and 3.

• 제어로봇시스템학회:학술대회논문집
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• 1994.10a
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• pp.494-497
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• 1994

We propose a novel velocity detection method of moving object based on a speckle pattern on the target surface using a self-mixing laser diode (SMLD). By this measurement, it was confirmed that the speckle signal has its waveform independent of the target velocity, and has its averaged frequency directly proportional to the target velocity. So it will be possible to detect the velocity of the target transversely translating against the laser light beam using a compact measuring system.

• Journal of the Korean Ceramic Society
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• v.30 no.2
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• pp.131-138
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• 1993

The effects of silane coupling agents on the injection molding process were investigated using silicone nitride mixtrues with a binder system containing polypropylene as a major binder (55vol% solid loading). The formation of bonding between silicon nitride powder and coupling agents was confirmed through the analyses of powder surface. The use of coupling agents improved mixing characteristics judged by the torque change during mixing process. the coupling agents also reduced molten viscosity of the mixture considerably, which is a main factor to determine the flow of the mixture. However, the bonding between coupling agents and polymers had a negative effect on the debinding process by retarding the thermal decomposition.

• International Journal of Ocean Engineering and Technology Speciallssue:Selected Papers
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• v.6 no.1
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• pp.15-21
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• 2003

A common feature in the three-dimensional numerical model experiments of coastal discharge with simplified model and idealized external forcings is investigated. The velocity fields due to the buoyancy and flaw flux, are spreaded radiately and the surface velocites are much greater than the homegeneous discharges. The coastal dischargd due to the Coriolis force and flaw flux are shaped a anticyclical gyre (clockwise) and determined the scale of the gyre in the coastal zone, respectively. The bottom topography restricts a outward extention of the coastal fronts and it accelerates a southward flow.

• Journal of the Korean Mathematical Society
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• v.45 no.1
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• pp.221-228
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• 2008

In this paper, we study the complexity of semigroup actions using complexity functions of open covers. The main results are as follows: (1) A dynamical system is equicontinuous if and only if any open cover has bounded complexity; (2) Weak-mixing implies scattering; (3) We get a criterion for the scattering property.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2012.11a
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• pp.81-83
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• 2012

친수성 코팅 물질인 폴리도파민을 초소수성표면에 패터닝함으로써 이차원 마이크로 플루이딕 장치 (폴리도파민 마이크로플루이딕 장치)를 만듦. 폴리도파민 마이크로플루이딕 장치는 마이크로 펌프가 없이 오직 중력만을 이용해서 액적의 이동을 가능케하는 기술임. 또한 이 장치는 매우 빠르게 액적을 손실없이 혼합시키는 기술임. 본 기술을 이용하여 금입자 반응 및 단백질 구조 분석을 수행함.

• Journal of the Korean Solar Energy Society
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• v.25 no.4
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• pp.133-139
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• 2005

In this paper, the ventilation performance of suspended particulates in indoor side was investigated by step-down method. Experiments were performed in function of mechanical ventilation types and locations of supply and extract air. The type 2 ventilation system shows the highest removal characteristics rather than other 2 types. It means that the displacement ventilation has also good decay rates of concentration compared to mixing ventilation.

• Proceedings of the KSME Conference
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• 2000.04b
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• pp.290-295
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• 2000

An experimental study was carried out in a cavity with upper channel and square heat surface by visualization equipment with Mach-Zehnder interferometer and laser apparatus. The visualization system consists of 2-dimensional sheet light by Argon-Ion Laser with cylindrical lens and flow picture recording system. Instant simultaneous velocity vectors at whole field were measured by 2-D PIV system(CACTUS'2000). Obtained result showed various flow patterns. Severe unsteady flow fluctuation within the cavity are remarkable and sheared mixing layer phenomena are also found at the region where inlet flow is collided with the counter-clockwise rotating main primary vortex. Photographs of Mach-Zehnder are also compared in terms of constant heat flux.