• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.62-62
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• 2019

Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1 and K2. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 pairedend read was used for GBS sequencing. GWAS was conducted using TASSEL 5.0. The TASSEL program uses a mixed linear model (MLM). The results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race and K2 resistant germplasm proportion remained between 66.67. The genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than 5 (K1 and K2) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. These SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races. The significant loci detected have also been illustrated and make the CPAS markers for NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

• Journal of Physiology & Pathology in Korean Medicine
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• v.33 no.2
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• pp.89-101
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• 2019

In recent times, the number of men with late-onset hypogonadism has increased, and interest on this topic has also increased. This study was conducted to investigate effects of the mixture extract of Fructus amomi Amari, Eucommiae cortex, Bombyx batryticatus on improve late-onset hypogonadism. The experimental subjects consisted of three groups: a control group consisting of 8-week-old male ICR mice that had undergone no treatment, an aging-elicited group (AE group) consisting of 50-week-old ICR male mice that had undergone no treatment, and a Mixed herbal extract treatment group (MT group) consisting of 50-week-old ICR male mice that had undergone the mixture extract of Fructus amomi Amari, Eucommiae cortex, Bombyx batryticatus treatment (0.1 g/kg/day) for 6 months. After the experiment, the mice from all the experimental groups were dissected, and they were analyzed through histochemical and immunohistochemical methods. The mixture extract of Fructus amomi Amari, Eucommiae cortex, Bombyx batryticatus reduces aging-induced cell damage and oxidative stress and increases the secretion of serotonin and B-endorphin in aged mice, and promotes spermatogenesis in seminiferous tubules and reduces apoptosis and oxidative stress, and increases androgen receptor, $17{\beta}-HSD$ and GnRH, increases the ratio of smooth muscle to collagen fibers in the corpus cavernosum, increases eNOS, decreases PDE-5 and oxidative stress in aged mice, so it improves depression, reproductive, sexual problems caused by Late-onset hypogonadism. the mixture extract of Fructus amomi Amari, Eucommiae cortex, Bombyx batryticatus inhibits the induction of osteoporosis by increasing decreased bone matrix distribution due to aging, increasing the activities of OPC and OPN, which are produced in osteoblasts, and decreasing RANKL, MMP-3 activity, increasing OPG activity. It also reduces muscle damage, oxidative stress, inflammation and apoptosis of muscle tissue, and increases Myo-D in the sartorius muscle of aged mice for improving muscle atrophy caused by by Late-onset hypogonadism.

• KIPS Transactions on Software and Data Engineering
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• v.11 no.3
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• pp.133-140
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• 2022

We investigate the performance of deep learning-based Korean language models on a task of predicting the score range of Korean essays written by foreign students. We construct a data set containing a total of 304 essays, which include essays discussing the criteria for choosing a job ('job'), conditions of a happy life ('happ'), relationship between money and happiness ('econ'), and definition of success ('succ'). These essays were labeled according to four letter grades (A, B, C, and D), and a total of eleven essay score range prediction experiments were conducted (i.e., five for predicting the score range of 'job' essays, five for predicting the score range of 'happiness' essays, and one for predicting the score range of mixed topic essays). Three deep learning-based Korean language models, KoBERT, KcBERT, and KR-BERT, were fine-tuned using various training data. Moreover, two traditional probabilistic machine learning classifiers, naive Bayes and logistic regression, were also evaluated. Experiment results show that deep learning-based Korean language models performed better than the two traditional classifiers, with KR-BERT performing the best with 55.83% overall average prediction accuracy. A close second was KcBERT (55.77%) followed by KoBERT (54.91%). The performances of naive Bayes and logistic regression classifiers were 52.52% and 50.28% respectively. Due to the scarcity of training data and the imbalance in class distribution, the overall prediction performance was not high for all classifiers. Moreover, the classifiers' vocabulary did not explicitly capture the error features that were helpful in correctly grading the Korean essay. By overcoming these two limitations, we expect the score range prediction performance to improve.

• Journal of the Korean earth science society
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• v.43 no.2
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• pp.265-282
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• 2022

This study analyzes the impact of long-range transport of biomass burning emissions from northeastern China on the concentration of particulate matter of diameter less than 10 ㎛ (PM₁₀) in Korea using the Weather Research and Forecasting model coupled with Chemistry (WRF-Chem). Korea was impacted by anthropogenic emissions from eastern China, dust storms from northern China and Mongolia, and biomass burning emissions from northeast China between April 4-and 7, 2020. The contributions of long-range PM₁₀ transport were calculated by separating biomass burning emissions from mixed air pollutants with anthropogenic emissions and dust storms using the zeroing-out method. Further, the radiation energy budget over land and sea around the Korean Peninsula was analyzed according to the distribution of biomass burning emissions. Based on the WRF-Chem simulation during April 5-6, 2020, the contribution of long-range transport of biomass burning emissions was calculated as 60% of the daily PM₁₀ average in Korea. The net heat flux around the Korean Peninsula was in a negative phase due to the influence of the large-scale biomass burning emissions. However, the contribution of biomass burning emissions was analyzed to be <45% during April 7-8, 2020, when the anthropogenic emissions from eastern China were added to biomass burning emissions, and PM₁₀ concentration increased compared with the concentration recorded during April 5-6, 2020 in Korea. Furthermore, the net heat flux around the Korean Peninsula increased to a positive phase with the decreasing influence of biomass burning emissions.

• Journal of Korean Society of Forest Science
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• v.111 no.3
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• pp.345-356
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• 2022

Yokjido is a 15-km² inhabited island located at the tip of the southeastern coast of the Korean Peninsula. Its forest is mostly composed of substitutional vegetation. Our aim was to provide basic information necessary for the conservation and management of the forest vegetation in Yokjido. We classified the types of existing vegetation using methods of the Zurich-Montpellier school of phytosociology. The resulting vegetation map shows the dominant tree species in the top canopy-layer. A total of 8 vegetation types were identified, which were arranged into a vegetation unit hierarchy of 2 communities, 4 sub-communities, 6 variants, and 2 subvariants. Evaluations of each type showed large and small differences in floristic composition, which reflect anthropogenic influences, site conditions, succession stages, and the establishment period. Moreover, vegetation types differed significantly in terms of species diversity indices; in particular, overall species richness, species diversity, and species evenness tended to increase significantly as the elevation increased. The herbaceous plant species showed the highest positive (+) correlation to x. These results were consistent with those of McCain, who reported that species diversity increases in mountainous areas with relatively low elevations due to the mid-domain effect. The forest succession in Yokjido will potentially enter a mixed-forest stage and then proceed to become an all-evergreen broad-leaved forest.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.27 no.3
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• pp.144-157
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• 2022

Noble gases, which are chemically inert and behave conservatively in marine environments, have been used as tracers of physical processes such as air-sea gas exchange, mixing of water masses, and distribution of glacial meltwater in the ocean. For precise measurements of Ne, Ar, and Kr, we developed a mass spectrometric system consisting of a quadrupole mass spectrometer (QMS), a high vacuum preparation line, an activated charcoal cryogenic trap (ACC), and a set of isotope standard gases. The high vacuum line consists of three sections: (1) a sample extraction section that extracts the dissolved gases in the sample and mixes them with the standard gases, (2) a gas preparation section that removes reactive gases using getters and separates the noble gases according to their evaporation points with the ACC, and (3) a gas analysis section that measures concentrations of each noble gas. The ACC attached to the gas preparation section markedly lowered the partial pressures of Ar and CO₂ in the QMS, which resulted in a reduced uncertainty of Ne isotope analysis. The isotope standard gases were prepared by mixing ²²Ne, ³⁶Ar, and ⁸⁶Kr. The amounts of each element in the mixed standard gases were determined by the reverse isotope dilution method with repeated measurements of the atmosphere. The analytical system achieved precisions for Ne, Ar, and Kr concentrations of 0.7%, 0.7%, and 0.4%, respectively. The accuracies confirmed by the analyses of air-equilibrated water were 0.5%, 1.0%, and 1.7% for Ne, Ar, and Kr, respectively.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.693-702
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• 2022

The purpose of this study is to present the problems of urban ecosystem imbalance in Seoul and the need to restore housing functions through comparison with Manhattan, New York, which grew earlier than Seoul and has been striving to solve urban problems and restore urban ecosystems. The residential status in urban commercial use areas was investigated through analysis of the total amount of residential space, characteristics of each type of residential building, and distribution of residential buildings. Through this, it can be seen that most of the space in Seoul's historic city center is biased toward commercial roads, and there are significantly insufficient high- and high-density mixed-use buildings suitable for urban dwellings compared to Manhattan's. In addition, it can be seen that the complex building in the historical city center of Seoul is located adjacent to the outskirts of the target site. In conclusion, Seoul's historical city center does not provide an appropriate residential space for creating an urban ecosystem, and it is necessary to come up with measures to restore housing functions.

• The Journal of Engineering Geology
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• v.33 no.1
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• pp.69-83
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• 2023

Forest fires increase the risk of subsequent soil erosion and mass movement in burned areas, even under rainfall conditions below landslide alert thresholds, by destroying plants and vegetation and causing changes to soil properties. These effects of forest fires can alter runoff in burned areas by altering soil composition, component minerals, soil water repellency, soil mass stability, and soil fabric. Heat from forest fires not only burns shallow organic matter and plants but also spreads below the surface, affecting soil constituents including minerals. This study analyzed X-ray diffraction and physical properties of topsoil and subsoil obtained from both burned and non-burned areas to identify the composition and distribution of clay minerals in the soil. Small amounts of mullite, analcite, and hematite were identified in burned soils. Vermiculite and mixed-layer illite/vermiculite (I/V) were found in topsoil samples from burned areas but not in those from non-burned areas. These findings show changes in soil mineral composition caused by forest fires. Expansive clay minerals increase the volume of soil during rainfall, degrading the structural stability of slopes. Clay minerals generated in soil in burned areas are therefore likely to affect the long-term stability of slopes in mountainous areas.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.7 no.2
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• pp.105-111
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• 2021

⁸⁹Zr is a positron-emitting radioisotope, which has known as well-suited radioisotope for use in a monoclonal antibody-based imaging agent for immuno-PET. The purpose of this study was to quantitatively evaluate the diagnostic ability of general trastuzumab and thio-trastuzumab as HER2 positive receptors based on Df hexadentate iron chelator. Desferrioxamine-p-SCN (Df-Bz-NCS) and desferroixamine-maleimide (Df-Mal) were purchased from Macrocyclics (Dallas, TX, USA). The trastuzumab was purchased from Roche (Schweiz), and thio-trastuzumab was obtained from professor Hyo-Jeong Hong group (Kangwon National University). The radioisotope ⁸⁹Zr was produced by domestic purification system and KIRAMS using medical cyclotron (50 MeV, Scantronix). The conjugates of Df-trastuzumab and Df-thio-trastuzumab were prepared with Df-Bz-NCS and Df-Mal under basic aqueous solution (pH 8-9) at room temperature, respectively. The conjugates purified by PD-10 column were mixed with dried ⁸⁹Zr chloride. ⁸⁹Zr-labeled conjugates were purified and concentrated by Amicon ultra centrifugal filter. The preparation step and time of ⁸⁹Zr-labeled conjugates was shorted as 4 steps within 2 hours. ⁸⁹Zr-labeled conjugates showed the highly radiochemical purity of over 98%, and were very stable until 7 days by the analysis of radio-ITLC method. Each radio-labeled conjugates were also exhibited the highly stability in both PBS buffer and mouse serum. Immuno-PET imaging of ⁸⁹Zr-labeled conjugates in mice bearing gastric cancer xenograft tumors with HER2 expression showed high tumor uptake in the NCI-N87 HER2-expressing. However, ⁸⁹Zr-Df-Mal-thio-trastuzumab showed a relatively lower tumor-to-background ratio than ⁸⁹Zr-Df-Bz-trastuzumab, as well as whole-body distribution. In the results, ⁸⁹Zr-Df-Bz-trastuzumab was evaluated to have a relatively higher HER2 diagnostic ability than ⁸⁹Zr-Df-Mal-thio-trastuzumab.

• Land and Housing Review
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• v.14 no.3
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• pp.93-106
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• 2023

This study attempted to simulate changes in the thermal environment according to the type of apartment complex in Korea using CFD techniques and evaluate the thermal environment by type of apartment. First, apartment complex types in the 2000s and 2010s were referred from previous studies and four types of apartment complex were extracted from. Second, the layout of the apartment complex and temperature changes were analyzed by the direction of wind inflow. Third, a standardized model was created from each type using tower type, plate type, and mixed driving. Last, CFD simulations were performed by setting up the inflow of wind from a total of eight directions. The temperature was relatively low in the type consisting of only the tower type and the type of placing the tower type in the center of the complex, regardless of the direction of the wind. It was due to the good inflow of wind from these types to the inside of the complex. It can be interpreted because wind flows easily into the complex in these types. The findings showed that wind flow and resulting temperature distribution patterns differed depending on the building type and complex layout type, confirming the need for careful consideration of the complex layout in the early design stage. The results are expected to be used as basic data for creating a sustainable residential environment in the early design stage of apartment complexes in the future.