• Korean Journal of Veterinary Service
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• v.37 no.3
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• pp.213-218
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• 2014

In early April 2014, a month-old Alexandrine Paraqeet (Psittacula eupatria) that was raised in a domestic aviary located in Gyungju-si, Korea was suddenly died and submitted to Animal Disease Intervention Center, Kyungpook National University in order to diagnose the causative agent. In post-mortem examination, the bird had abnormally developed feathers on the neck and abdomen region and subcutaneous hemorrhages on the neck and cheek adjacent to the beak. At necropsy, the bird had hemorrhage on the muscle of the femoral region, ascites, multi-focal hemorrhages on the epicardium, and diffuse hemorrhages on the sub-serosa of proventriculus and gizzard, suggesting typical avian polyomavirus (APV) infection. The partial large tumor (T) antigen gene of APV was detected by PCR from tissues of the heart, lung, liver, kidney, proventriculus and feathers of the APV-suspected birds. However, other pathogenic virus-specific nucleic acid common with psittacine birds such as avian bornavirus, psittacine beak and feather disease virus and psittacid herpesvirus were not detected from the mixed tissue samples of the bird, indicating this case is due to single infection of APV. Nucleotide sequence analysis of the partially amplified large T antigen DNA was confirmed to have 99~100% homology with that of the previously reported APV strains. This case report describes the first detection of APV in Alexandrine Paraqeet in Korea.

• Korean Journal of Veterinary Research
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• v.49 no.2
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• pp.157-161
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• 2009

A 11-year-old female mixed cat with subcutaneous mass around the left 5th mammary glands was presented to local animal hospital. According to history taking, the mass recurred 2 times on the same site of abdomen. After surgical excision, subcutaneous mass was referred to Pathology Department of Veterinary Medicine in the Jeju National University. Grossly, round to oval, milky yellow or pale red nodules, measuring 0.1${\sim}$1 cm in diameter, were occupied in the subcutis. Microscopically, the most neoplastic sweat glands were proliferated in the dermis and subcutis. Most tubules were lined by round to oval shaped epithelium with eosinophilic cytoplasm, hyperchromatic nuclei with high mitotic figures and severe central necrosis. The neoplastic epithelium also had periodic acid-Schiff-positive diastase-resistant cytoplasmic granules, but was negative for Perl's iron stain. Based on the gross, histopathologic and special staining, this cat was diagnosed as apocrine sweat gland adenocarcinoma. In our best knowledge, this is the first report of apocrine sweat gland adenocarcinoma around abdominal mammary gland in a cat.

• Journal of the korean academy of Pediatric Dentistry
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• v.39 no.1
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• pp.97-102
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• 2012

Odontoma is a mixed tumor formed by the overgrowth of ectodermal epithelial cells and mesodermal cells. It arises during normal tooth development and only grows to a certain level. It is not a true neoplasm and therefore it is considered as hamartomas. Odontomas are classified into compound and complex types based on morphology. Compound odontoma is commonly found in the anterior region of the maxilla, and has a radiopaque lesion that resembles small teeth. Usually, odontoma is treated by conservative surgical removal and there is little probability of recurrence. This paper describes three cases of compound odontoma diagnosed in children with 1 year of follow-up. These case reports present patients having eruption disturbance of primary and permanent teeth due to the presence of compound odontoma. In order to induce spontaneous eruption, conservative surgical removal was performed showing favorable results.

• Journal of Ginseng Research
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• v.44 no.4
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• pp.619-626
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• 2020

Background: The effects of diol-ginsenoside fraction (Diol-GF) and triol-ginsenoside fraction (Triol-GF) from Korean Red Ginseng on the development of type 1 diabetes (T1D) were examined in diabetes-prone biobreeding (DP-BB) rats that spontaneously develop T1D through an autoimmune process. Methods: DP-BB female rats were treated with Diol-GF or Triol-GF daily from the age of 3-4 weeks up to 11-12 weeks (1 mg/g body weight). Results: Diol-GF delayed the onset, and reduced the incidence, of T1D. Islets of Diol-GF-treated DP-BB rats showed significantly lower insulitis and preserved higher plasma and pancreatic insulin levels. Diol-GF failed to change the proportion of lymphocyte subsets such as T cells, natural killer cells, and macrophages in the spleen and blood. Diol-GF had no effect on the ability of DP-BB rat splenocytes to induce diabetes in recipients. Diol-GF and diol-ginsenoside Rb1 significantly decreased tumor necrosis factor α production, whereas diol-ginsenosides Rb1 and Rd decreased interleukin 1β production in RAW264.7 cells. Furthermore, mixed cytokine- and chemical-induced β-cell cytotoxicity was greatly inhibited by Diol-GF and diol-ginsenosides Rc and Rd in RIN5mF cells. However, nitric oxide production in RAW264.7 cells was unaffected by diol-ginsenosides. Conclusion: Diol-GF, but not Triol-GF, significantly delayed the development of insulitis and T1D in DP-BB rats. The antidiabetogenic action of Diol-GF may result from the decrease in cytokine production and increase in β-cell resistance to cytokine/free radical-induced cytotoxicity.

• Journal of Veterinary Clinics
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• v.24 no.1
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• pp.26-28
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• 2007

An 11-year-old castrated male, Shih-tzu dog was admitted for progressive weight-bearing lameness and mass on the right hindlimb. Radiography and ultrasonography revealed a large well-marginated multinodular mass with mixed echogenicity. On T1 and T2-weighted magnetic resonance imaging (MRI), the mass has a heterogenous signal intensity similar to or higher than muscle. The masses were extended to the pelvic cavi쇼 through obturator foramen and displaced the rectum dorsally. It was diagnosed as synovial sarcoma which was composed with mesenchymal and epithelial elements on histopathological findings of the multifocal biopsied specimen. MRI was helpful to determine the definitive margin for surgical resection of the mass. The mass was recurred at the 6th month after surgery. On the 10th month, the patient was euthanasiuzed by owner's request.

• Journal of Veterinary Clinics
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• v.35 no.3
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• pp.107-110
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• 2018

A 5-year-old Thoroughbred race horse was presented to Busan Korea Racing Authority equine hospital with a 3-year history of a slow-growing left rostral maxillary mass. The location and progressive growth of the mass eventually resulted in poor food prehension, quidding and mouth bit placement. The mass was solitary and hard, and covered by normal smooth oral mucosa. Radiographic examination of the maxillae showed a flocculated and mixed radiolucent lesion protruding outward and displacing the 202 and 203 teeth caudally. The 202 tooth was in normal size and the 203 tooth was hypoplastic on radiography. Under general anesthesia, a partial surgical resection of the mass was performed to minimize functional loss and facilitate prompt return to track. After surgery, there was improvement in food intake, mouth bit placement, and cosmetic appearance. Histopathological examination determined the resected maxillay mass to be an ossifying fibroma. However, there was continued growth of remnant mass in the maxilla. Equine ossifying fibroma is a rare condition and primarily affects the rostral mandible, and less commonly, the maxillae. In this case, the lesion was slow-growing, and caused cosmetic and functional impairments, including poor food intake and reduced trainability. Surgical resection was performed, but the effect of treatment was limited due to advanced size / stage of the tumor. Early dental care is suggested for horse owners to prevent belated identification and improve successful treatment of oral disorders like ossifying fibroma.

• Korean Journal of Veterinary Research
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• v.45 no.3
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• pp.369-373
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• 2005

A male mixed breed dog with unknown age (case 1) and 5-year-old female golden retriever (case 2) were admitted to the Department of Veterinary Pathology at College of Veterinary Medicine, Seoul National University after sudden death. At necropsy, stifle region of case 1 was replaced by coalescing firm tan nodules approximately 1-3 cm in diameter. Neoplastic nodules were also presented in the lung. In case 2, the spleen was enlarged 3-5 times its normal size and numerous firm tan nodules were noted in the kidney, pericardium, diaphragm and lymph nodes. Histopathologically, the neoplastic masses of case 1 consisted of plump spindle cells having abundant cytoplasm and indistinct cell borders that formed interlacing bundle. In case 2, the masses were consisted of round to polygonal cells with abundant eosinophilic cytoplasm and eccentric nuclei. Numerous multinucleated giant cells containing nuclei of various sizes were noted. Immunohistochemically, neoplastic cells of all two cases stained intensely with vimentin and lysozyme. Based on the gross and light microscopic findings together with immunohistochemistry, both cases were diagnosed as localized histiocytic sarcoma with pulmonary metastasis and disseminated histiocytic sarcoma, respectively.

• Journal of Biomedical Engineering Research
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• v.32 no.3
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• pp.185-190
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• 2011

The purpose of this study is to investigate the influence of organism vigor information solution(OVIS) on the animal cell growth and to set up a condition for cell culture. We investigated the reactions on the MG-63 and MCF-7 cell line in mixture culture media with various amount of REVIEW solution, which is an example among various OVIS. DMEM-HG was used as basic media. The concentration range of the mixture was limited from 0% to 15%. MTT assay is used for cell viability test. The cell was incubated for 14days and the MTT assay was performed on day 1, 3, 7, 10 and 14 throughout the experiment. We used the ELISA reader to measure the Optical Density(O.D) at 595 nm wavelength filter. MCF-7 was linearly proliferated according to culture time and concentrations of OVIS. On the other hand, MG-63 cells were measured the highest O.D value at 12%. The growth rates of both cells cultured in mixed culture media with OVIS are much higher than those in only basic media, DMEMHG, after 14days. It was confirmed that cell cultured at OVIS grows rapidly at certain period although cells showed a negative effect in initial stage.

• Korean Journal of Plant Resources
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• v.24 no.6
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• pp.712-718
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• 2011

Ginseng (Panax ginseng) is frequently used in Asian countries as a traditional medicine. The major components of ginseng are ginsenosides. Among these, ginsenoside compound K has been reported to prevent the formation of malignancy and metastasis of cancer by blocking the formation of tumor and suppressing the invasion of cancer cells. In this study, ginsenoside $Rb_1$ was converted into compound K, via secreted ${\beta}$-glucosidase enzyme from the Leuconostoc lactis DC201 isolated, which was extracted from Kimchi. The strain DC201 was suspended and cultured in MRS broth at $37^{\circ}C$. Subsequently, the residue from the cultured broth supernatant was precipitated with EtOH and then dissolved in 20 mM sodium phosphate buffer (pH 6.0) to obtain an enzyme liquid. Meanwhile, the crude enzyme solution was mixed with ginsenoside $Rb_1$ at a ratio of 1:4 (v/v).The reaction was carried out at $30^{\circ}C$ and 190 rpm for 72 hours, and then analyzed by TLC and HPLC. The result showed that ginsenoside Rb1 was transformed into compound K after 72 hours post reaction.

• The Korean Journal of Mycology
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• v.11 no.4
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• pp.151-157
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• 1983

To find antitumor components in Korean basidiomycetes, the carpophores of Armillariella mellea which were collected in Gyeong Gi Province were extracted with distilled water at $90{\sim}100^{\circ}C$ for eight hours. The hot water extract was concentrated under reduced pressure, mixed with three-fold volumes of ethanol and allowed to stand at $4^{\circ}C$ overnight. The precipitate was centrifugated and lyophilized to yield a protein-polysaccharide fraction. It was examined for antitumor activity against sarcoma 180 implanted in ICR mice. The fraction showed 75.7%, 83.9%, and 94.1% of tumor inhibition ratios at the doses of 10, 20 and 50 mg/kg/day, respectively. The chemical analysis of the fraction showed that it contained a polysaccharide(41.3%) and a protein (35.0%). The hydrolyzates of the polysaccharide moiety contained fucose (4.5%), xylose (1.1%), galactose (17.4%), glucose (55.4%), mannose(19.4%), and one unknown monosaccharide. The protein moiety contained seventeen amino acids. The protein-polysaccharide from A. mellea was administered, i.p., to mice and caused an influx of polymorphonuclear leukocytes (PMN) at $5{\sim}24$ hours which was followed by an accumulation of macrophages and disappearance of the PMN at $48{\sim}72$ hours.