The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Studies on Constituents of the Higher Fungi of Korea(XXXVII) - Antitumor Components of Armillariella mellea -

한국산(韓國産) 고등(高等) 균류(菌類)의 성분(成分) 연구(硏究)(제(第)37보(報)) - 뽕나무버섯의 항암(抗癌) 성분(成分) -

  • Kim, Jin-Sook (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Choi, Eung-Chil (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Kim, Hye-Ryoung (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Lee, Chong-Kil (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Lee, Chong-Ock (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Chung, Kyeong-Soo (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Shim, Mi-Ja (Seoul City University) ;
  • Kim, Byong-Kak (Department of Microbial Chemistry, College of Pharmacy, Seoul National University)
  • 김진숙 (서울대학교 약학대학 미생물약품화학교실) ;
  • 최응칠 (서울대학교 약학대학 미생물약품화학교실) ;
  • 김혜령 (서울대학교 약학대학 미생물약품화학교실) ;
  • 이종길 (서울대학교 약학대학 미생물약품화학교실) ;
  • 이정옥 (서울대학교 약학대학 미생물약품화학교실) ;
  • 정경수 (서울대학교 약학대학 미생물약품화학교실) ;
  • 심미자 (서울시립대학) ;
  • 김병각 (서울대학교 약학대학 미생물약품화학교실)
  • Published : 1983.12.30
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Abstract

To find antitumor components in Korean basidiomycetes, the carpophores of Armillariella mellea which were collected in Gyeong Gi Province were extracted with distilled water at $90{\sim}100^{\circ}C$ for eight hours. The hot water extract was concentrated under reduced pressure, mixed with three-fold volumes of ethanol and allowed to stand at $4^{\circ}C$ overnight. The precipitate was centrifugated and lyophilized to yield a protein-polysaccharide fraction. It was examined for antitumor activity against sarcoma 180 implanted in ICR mice. The fraction showed 75.7%, 83.9%, and 94.1% of tumor inhibition ratios at the doses of 10, 20 and 50 mg/kg/day, respectively. The chemical analysis of the fraction showed that it contained a polysaccharide(41.3%) and a protein (35.0%). The hydrolyzates of the polysaccharide moiety contained fucose (4.5%), xylose (1.1%), galactose (17.4%), glucose (55.4%), mannose(19.4%), and one unknown monosaccharide. The protein moiety contained seventeen amino acids. The protein-polysaccharide from A. mellea was administered, i.p., to mice and caused an influx of polymorphonuclear leukocytes (PMN) at $5{\sim}24$ hours which was followed by an accumulation of macrophages and disappearance of the PMN at $48{\sim}72$ hours.

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