• 한국미생물·생명공학회지
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• 제45권3호
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• pp.257-264
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• 2017

혼합유박은 황화물, 암모니아 그리고 아민이 발생하는 것으로 알려져 있다. 악취 저감 미생물을 선별하기 위해 물과 혼합유박이 포함된 바이얼을 농화 배양하였다. 황화물 저감 미생물의 분리를 위해 황화수소 및 메틸메르캅탄 저감 활성실험을 수행하였다. 대조군에는 100 ml 바이알에 혼합유박(0.25 g)과 증류수(10 ml)를 넣어 악취를 발생시켰으며 실험군에는 대조군 바이알과 같은 상태에서 분리균주를 접종하였다. 분리균주 중에서 황화합물의 저감효율이 가장 높은 균주를 일반적인 동정 방법인 16S rRNA sequence 분석으로 동정 결과 Brevundimonas diminuta로 동정되었으며 KCTC에 기탁하여 기탁번호 KCTC11724BP를 부여받았다. B. diminuta는 황화수소 표준가스를 200 ppmv까지 24시간내에 모두 제거하였다. 또한, 황화수소와 메틸머캅탄의 최대 제거 효율은 바이알 실험에서 453 ppmv과 98 ppmv에서 각각 100% 효율을 나타냈다. 또한 돈분을 이용한 악취발생반응기에서는 접종량 20% (v/weight of swine manure)일 때 황화물 95% 이상 제거 효율을 나타냈다.

• 박물관보존과학
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• 제8권
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• pp.13-24
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• 2007

미술부에서는 "한·중 도자교류전" 연구집에 개제할 목적으로 소장품 중 한·중 금채자기의 금채기법을 보존과학팀에 조사의뢰하였다. 대상 소장품은 금을 사용하여 도자기 유약 층을 장식한 개성 106 청자상감수하원문금채편호 고려청자 1점과 중국 송나라시기에 제작된 본관 10011 천목다완, 본관2027 시유초화접문화금완, 덕수3322시유완이다. 조사 결과, 금채장식의 안정화 방법은 고운 금분에다 매용제 성분을 혼합하여 채식을 한 후 저온에서 번조한 것은 유사하였지만, 채식처리방법에서는 다소 차이가 있었다. 즉, 고려 화금청자는 화려한 상감기법 위에 세필로 일일이 금채장식을 하였다면 중국 화금자기는 아무런 장식이 없는 도자기 유약면에 나비나 당초문을 스텐실 기법으로 장식한 것이 큰 차이이다. 이런 조사결과에 따라 일부 박락된 금채 부분을 새롭게 복원한 결과, 제작 당시 화금자기가 얼마나 화려한 자기이었는지를 확인할 수가 있었다.

• 한국임상수의학회지
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• 제16권2호
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• pp.375-380
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• 1999

This study was conducted to develop an intrauterine inseminator (IUI) to deposit of frozen semen into uterus and to evaluate the results obtained after artificial insemination by IUI. Two Japanese spitzs (2 to 4 years of age) were used as semen donors. Semen was collected by manual masturbation into sterile glass collection tubes and separated into 3 fractions with only the sperm-rich fractions retained for further examination. Sperm motility >70%, sperm concentration of 200 to $400{\times}10^6 cells/ml$$\times$g for 5 min and poured out the suspended solution, and then diluted with 2 ml Tris-buffer which was consisted of 2.4 g Tris, 1.4 g citric acid, 0.8 g glucose, 0.1 $\mu\textrm{g}$/ml streptomycin, 100 IU/ml penicillin, 20 ml egg yolk to 100 ml mili-Q water (Ext I) or supplemented with 8 ml glycerol and 1 ml Equex STM paste to 100 rnl (Ext II). The diluted semen was cooled to 5$^{\circ}C$ in cold room, where the temperature in the sample reached 5$^{\circ}C$. Two h after beginning the cooling procedure, 2 ml of Ext II, also at 5$^{\circ}C$, was added and mixed by gently reversing the tubes several times during 1 h. The final sperm concentration for freezing was approximately $50{\times}10^6 cells/ml$. After equilibration, the semen was loaded into 0.5 ml straw and frozen on the liquid nitrogen vapour in styrofoam box. The straws were thawed at 7$0^{\circ}C$ for precisely 6 sec. After thawing of each straw, the frozen semen can survived over 50% motility. All the females were inseminated twice with 1 ml of $25{\times}10^6 cells/ml$

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.73-73
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• 2018

Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1, K2, K3 and K3a. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 paired- end read was used for GBS sequencing. GWAS was conducted using T ASSEL 5.0. The T ASSEL program uses a mixed linear model (MLM). T he results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race, while the least number of accessions (34.37%) resisted K3a race. For races K2 and K3, the resistant germplasm proportion remained between 66.67 to 70.83%. T he genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than5 (K1 and K2) and more than4 (K3 and K3a) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. T hese SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races, whereas on chromosome 4, 6, 11, and 12 for K3 and K3a races. The significant loci detected have also been illustrated, NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

• 한국환경생태학회지
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• 제26권2호
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• pp.264-272
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• 2012

본 연구는 생태관광지의 유형별 탐방객 특성을 파악하기위해 북한산둘레길, 우포늪, 지리산둘레길, 울진금강소나무 숲길을 대상으로 탐방객의 인구통계적 특성 및 여행동기, 훼손에 대한 인식 등을 분석하였다. 각 대상지를 방문한 탐방객들을 대상으로 1:1 면접조사를 통해 현장 설문조사를 실시하였다. 북한산둘레길의 경우 인근 주민들이 건강을 위해 찾는 빈도가 높고 생태관광객의 비율이 낮은 경향을 보였으며, 환경훼손에 대한 인식도 높지 않게 나타났다. 우포늪은 동료간의 친목을 다지기 위해 단체로 움직이는 탐방객 수가 많았고, 습지생태계의 관찰·체험, 탐조활동 등 생태관광 비율이 높게 나타났으며, 산악형에 비하여 물리적인 훼손요인이 많지 않아 훼손에 대한 탐방객들의 인식은 높지 않았다. 지리산둘레길은 생태관광객과 함께 일반 대중관광객과 자연관광객의 비율도 높게 나타났으며, 평지형 관광지보다 환경훼손에 대한 인식이 높았다. 울진금강소나무숲길의 경우 탐방을 제한함으로써 생태관광에 대한 인식이 높고 자연환경 보전의식이 높은 관광객들이 주로 방문하기 때문에 거의 모든 지표에서 다른 생태관광지에 비해 높은 값을 보였다. 관광지의 자연환경훼손이 우려되는 경우에 입장료 징수 또는 탐방예약제를 통해 관광객수를 조절하는 방안에 대해서는 대체적으로 긍정적으로 받아들이는 것으로 조사되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권6호
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• pp.811-815
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• 2006

The objective of this study was to evaluate the effect of electric fan installation for milk production improvement of dairy cattle in Thailand. The study was conducted using 2 small-holder dairy farms in Chiang Mai province, during April to August 2004. Electric fans were installed in front of each row of cows. Each of the two rows of cows in the barn was defined as an experimental unit, thus each farm had two experimental units. The fans were operated alternately in 7-day intervals between rows of cows within each farm during the day or between 8.00 am to 8.00 pm. Non-operation periods were used as control. Milk yields were recorded. Data on environmental temperature and humidity were obtained from Chiang Mai Meteorological Center. Result from statistically analysis of milk record suggested an interaction between lactation period and fan installation. Therefore, this interaction term of lactation period and fan installation (PERIOD_FAN) was added as a variable to the regression model. Due to the repeated data collection of milk yield from the same cow (alternate week), milk yield was analyzed by repeated measure analysis (Mixed model). Least square means were calculated for all levels and used to compare between each pair-wise values. The final data were collected from the total of 18 cows with 2,072 data. Overall means and SEM of milk yields and days in milk separated into farm were $14.7{\pm}0.06kg/day$ and $176.3{\pm}2.2days$, and $15.2{\pm}0.22kg/day$ and $202.5{\pm}3.7$ days for farm A and farm B, respectively. For multivariable analysis, only PERIOD_FAN and humidity were significantly associated with milk yield. Only the first period of lactation showed that the amount of milk yields during fan installation was higher than that of non-fan installation (p<0.05). Cows with fan installation produced approximately 1.2 kg/cow more milk than cows without fan installation during this period. In conclusion, the use of electric fan operated during the day time increased milk production of cows during the first period of lactation.

• 패션비즈니스
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• 제11권6호
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• pp.87-100
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• 2007

This study aims at analyzing the characteristics of deconestructionism expressed in Issey Myake's fashion who is one of the most creative designers in the world. Method and contents of this study are as follows. Through the review of literatures, three major categories such as interminacy of meaning, decentring, and intertextuality and eight subcategories have been identified as major features of deconestructionism. These subcategories for content analysis were selected to examine the characteristics of deconstructionism expressed in Issey Myake's design in depth. Interminacy of meaning was divided into unstructured and unconstructed factors while decentring into qualities of cross-gender, subculture, anti-fashion, and post-humanism. Lastly, intertextuality was classified into factors of disordered mixing mode and mixed styles. Materials for analysis were chosen from total 349 designs on the website,www.firstview.com, which carries Issey Miyake's collection from 2002 S/S through 2006 F/W. 2 fashion major analysts including the researcher participated in 1st analysis and two another fashion major analysts participated in 2nd analysis. The final level of consistency between analysts was over 97% for all categories. The data analysis quantitatively evaluates the frequency of each category and qualitatively evaluates the characteristics of design. The result is as follows. First, the avant-garde facets of inter-textuality are most prominent. In particular, Miyake has introduced the various novelly textiles to fashion and employed the mixtured styles, not to copy them but to create hybrid designs by using pastiches and mixing fashion styles. Moreover, he has used various fabrics in order to inflict shock and contrast but still create subtle harmony, thus enriching his fashion. Second, the indeterminacy of meaning is the second most frequently shown facet of Miyake's fashion design. Miyake has employed unconstructed expression techniques, which has projected unusual images free from basic forms of designs and also has used the unstructured styles which dismantle harmony and balance-the common rule of design. He has made a new attempt to acknowledge clothing as an expressive artwork by allowing clothes to take a complete form when a person actually wears them. He has demonstrated a unique perspective on cloth construction and presented a love for artistic collaboration in the development of his collections A distinct manufactured aesthetic is exaggerated, even hyperbolic. Thirdly, the aspects of decentralization were also shown in Miyake's design, however, it has been visibly decreased after 2002 S/S.

• 한국측량학회지
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• 제27권5호
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• pp.545-553
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• 2009

국가 공간정보 중 지형의 형태를 나타내기 위해 수치표고모형(DEM)을 사용한다. 수치표고모형을 생성하기 위한 다양한 방법 중 항공 LiDAR를 활용한 방법은 지형의 변화에 신속하게 자료를 획득할 수 있고, 사진측량기법에 비해 기상조건에 따른 제약을 덜 받는다. 이러한 항공 LiDAR는 최근 국가공간정보의 제작에 다양하게 활용되기 시작하였으나, 다양한 식생이 우거진 한반도의 산림지역에서 일반적으로 평가되고 있는 항공 LiDAR 측량의 정확도가 얻어지는 지에 대한 연구는 아직 이루어지고 있지 않다. 본 논문에서는 다양한 식생이 존재하는 산림지역을 연구대상지역으로 선택하여 수목의 특성에 따라 항공 LiDAR 자료의 정확도가 어떻게 다른지 평가하고자 하였다. 연구대상지역은 낮은 식생이 존재하는 나대지를 포함하고 있는 지역과 이 나대지의 경계부분과 인접하고 있는 다양한 수목이 존재하는 산림지역을 선정하였다. 선정된 지역에 대하여 대상지역에 고르게 분포하도록 검사점을 선점하고 GPS 측량을 실시하였다. 이렇게 측량된 검사점과 항공 LiDAR 자료와의 비교를 통하여 정확도를 평가하였다. 정확도 평가 결과, 활엽수 지역에서는 상당히 큰 오차가 발생할 수 있기 때문에 항공 LiDAR 측량에서도 촬영시기를 낙엽이 떨어진 이후로 하여야 함을 알 수 있었다.

• 복식
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• 제56권6호
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• pp.1-15
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• 2006

The purpose of this study is to present a new viewpoint in look by closely examining fashion and look through the past, present, and future. Specifically, the concept of time is studied and fashion is considered based upon the concept of time, finally, looks in fashion related to time are analysed. For this study, the literature of retro, modern, contemporary, futuristic looks that express the past, present, and future, and the classic look, the popularity of which transcends the concept of time was considered focusing on 20th century women's fashion. Design characteristics of these looks were analyzed utilizing photographs of collection images. The results of the study are as follows: first, although the retro look of different periods regularly rotate and appear, they change and develop in new and various ways depending on the Periodic background of the time they appear in, and thus show simultaneously the cyclical and straight nature of time. Second, the modern look includes not only the moaning of time concept of the present, but also of interpreting anew the formal significance of modernism in the present. In other words, because it cyclically repeats the times of past modern periods, the look can be said to focus on the cyclical nature of time. Third, the contemporary look is a style that expresses current time most exactly as it exists, and it can be said to be the look in which current time is most vividly expressed. Fourth, the time concept in futuristic look is a subjective time that selectively accommodates and expresses the objective time of the future which has not yet come, and that can be the look's most important point. Last, the classic look possesses an objective value that transcends the concept of time, and it keeps returning, showing the cyclical nature of time. The closer this study came to the present, it could be seen that a look communicated more complex meanings, Influenced by periodic phases such as diversification, individualization, and eclecticism, and that while it could not be defined as any one look, various elements were expressed eclectically by being mixed and matched with each other, and it could utilize more natural forms, colors, and materials.

• 한국균학회지
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• 제31권1호
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• pp.1-7
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• 2003

Beauveria속균은 2000년 9월 1일부터 2002년 8월 31일까지 총 151개 표본이 채집되었고 분리된 균주는 25개다. 분리된 균주는 대부분 Beauveria bassiana에 속하는 것으로 나타내었다. 춘천시 삼악산에서 채집한 유충노랑곰보 동충하초의 특성은 기주를 균사에 의하여 쌓여있으며 자좌는 기주로부터 하나 내지 4개가 나오며 분생포자도 같이 형성되었다. 밝은 노란색의 자좌는 45 mm 이고 머리는 $17mm{\times}4mm$ 이고 자루 28mm 이지만 경계는 뚜렷하지 않았다. 자낭각은 머리에 조밀하게 분포되어 있고 묻힌형이고 크기는 $530{\sim}550{\times}290{\sim}300{\mu}m$이었고 자낭은 $400{\sim}450{\times}4{\sim}5{\mu}m$이었다. 자낭포자는 실 모양이고 2차 포자로 분열한 후 바로 둥근 2차 포자를 형성하였다. Cordyceps staphyl- indaecola의 불완전 세대는 형태적인 특징인 분생자경은 rachis로 분생포자세포는 정단으로 생장하였으며 작은 목에서부터 원형의 분생포자를 형성하였다. 크기는 $2.6{\sim}3.4{\times}1.2{\sim}1.9{\mu}m$로 Beauveria bassiana로 동정하였다. 균사생육에 가장 적합한 배지는 HM이었고 기본 액체배지로는 HM, MCM 배지에서 우수한 건조 균체량을 나타내었다. 11일간 배양하여 $25^{\circ}C$에서 가장 우수한 생장과 밀도를 나타냈으며 $pH6.5{\sim}pH8.5$까지 배지에서 균사생장이 양호하였다. 현미와 번데기 배지에서 균사가 배지의 표면을 채우는 데 일주일이 걸리며 15일이나 18일이 되면 밝은 균사집합체에서 분생자병속이 형성되기 시작하였고 40일 후에는 분생자병속에 분생포자가 형성되기도 하나 간혹 자낭각이 형성되는 자좌도 있었다. 배양에서 형성된 자실체와 자연에서 채집한 자실체는 형태적으로 비슷하나 인공적으로 머리부분에서 자낭각이 형성하게 하는 것은 매우 어려운 것으로 나타났다.