• Title/Summary/Keyword: Mitochondrial cytochrome b gene

Search Result 110, Processing Time 0.035 seconds

우리나라 긴꼬리닭의 계통분류학적 추정

  • Yeon, Seong-Heum;Jo, Chang-Yeon;Kim, Jong-Dae;Jin, Hyeon-Ju;Lee, Seung-Su;Kim, Yeong-Geun;Sang, Byeong-Don
    • Proceedings of the Korea Society of Poultry Science Conference
    • /
    • 2006.11a
    • /
    • pp.84-85
    • /
    • 2006
  • This study was carried out to ascertain phylogenetic status of long-tail chicken which found recently in Korea and was presumed to be a kind of Korean Natives. 10 loci microsatellites were analysed for 449 birds of 11 groups and 2 region of mitochondrial DNA were sequenced for 135 birds of the same groups, that consist of 3 introduced breeds and 8 Korean Natives including 3 long-tail chicken. In mean numbers of alleles per locus(MNA) for microsatellites, long-tail chicken were smaller (2.60${\sim}$3.20) than the others, but in heterozygosities, were higher(0.4087${\sim}$0.5375) than others that were the same level of MNA. And in the neighbor joining bootstrap tree drawing by Nei's standard distance, they made a cluster with some Korean Native groups. All of the nucleotide sequences of mitochondrial cytochrome b gene and D-loop were classified into 23 haplotypes. In long-tail chicken, the haplotypes were 3 kinds, and were different among the groups (LTA, LTB and LTD). Resultly, it was supposed that 3 groups of the long-tail chicken be all a kind of Korean Natives.

  • PDF

Genetic Variation in the Asian Shore Crab Hemigrapsus sanguineus in Korean Coastal Waters as Inferred from Mitochondrial DNA Sequences

  • Hong, Sung-Eic;Kim, Jin-Koo;Yu, Jeong-Nam;Kim, Keun-Yong;Lee, Chung-Il;Hong, Kwan-Eui;Park, Kie-Young;Yoon, Moon-Geun
    • Fisheries and Aquatic Sciences
    • /
    • v.15 no.1
    • /
    • pp.49-56
    • /
    • 2012
  • Genetic variation in the Asian shore crab Hemigrapsus sanguineus was determined from partial mitochondrial DNA (mtDNA) sequences of the cytochrome b (Cytb) gene. Samples included 143 crabs from six localities along three coastlines in South Korea. A nucleotide sequence analysis revealed 38 variable sites in a 470-bp sequence, which defined 37 haplotypes. The haplotypes were not associated geographically and had a shallow genealogy. Pairwise $F_{ST}$ tests and a two-dimensional scaling analysis revealed no significant genetic differentiation among most of the populations. The low pairwise comparison values, but significant genetic differentiation of a northeastern population from all other populations, might have been influenced by a restriction in gene flow caused by hydrographic conditions such as ocean boundaries. The high haplotype diversity, low nucleotide diversity, and time since H. sanguineus expansion in Korean coastal waters indicate rapid population growth and a recent, sudden expansion in the Late Pleistocene.

Multiple Age-Associated Mitochondrial DNA Deletions in Mouse Brain

  • Kim, Jin-Sun;Kim, Min-Jung;Kwon, In-Sook;Song, Eun-Sook
    • BMB Reports
    • /
    • v.30 no.1
    • /
    • pp.33-36
    • /
    • 1997
  • Age-dependent deletion of mitochondrial DNA (mtDNA) was detected in mouse brain using PCR method. The size of the deleted fragment was 0.5 kb, 0.9 kb. 1.7 kb and 4.3 kb in the region between cytochrome b gene and ATPase 6 gene. The deleted fragment was increased gradually from 3-month to 22month Direct repeat sequence flanking the deletion in 0.5 kb PCR product was TAAT.

  • PDF

Sequence Diversity of Mitochondrial Cytochrome b Gene in Grey Goral Naemorhedus caudatus(Artiodactyla, Bovidae) from Korea (한국의 산양(우제목, 소과)의 미토콘드리아 Cytochrome b 염기서열 다양성)

  • Koh, Hung-Sun;Yang, Byong-Guk;Lee, Bae-Kun;Lee, Jong-Hyong
    • Animal Systematics, Evolution and Diversity
    • /
    • v.18 no.1
    • /
    • pp.13-21
    • /
    • 2002
  • To add genetic information to the conservation efforts on grey coral (Naemorhedus caudatus) in Korea, we investigated the pattern of mitochondrial cytochrome b gene sequence (606 bp) of six specimens from two localities in Korea. The corresponding sequences of N. caudatus in China obtained from GenBank were also used. The nucleotide Tamura-Nei distances between each of four haplotypes of N. caudatus in Korea and the haplotype of N. caudatus in China varied from 0.0650 to 0.0803: N. caudatus revealed high level of sequence diversity in Bovidae. In N. caudatus in Korea, the distances among three haplotypes at Yanggu were 0.0151 to 0.0185, and it suggests that the genetic diversity of Yanggu population was decreased in low level. Moreover, the distances between each of three haplotypes at Yanggu and one haplotype at Samcheok were 0.0343 to 0.0489. It indicates that habitat isolation caused the continuous increase of genetic distance with geographic distance in N. caudatus, and various conservation plans for mitigating the loss of genetic diversity in Korea have to be in immediate action. To clarify the taxonomic status of N. caudatus, the sequence (276 bp) of N. goral available from GenBank were also utilized, and n goral was not distinct from N. caudatus. It suggests that they may be conspecific, but further analyses with additional specimens of two species are necessary.

Genetic Variation of Taenia Pisiformis Collected from Sichuan, China, Based on the Mitochondrial Cytochrome b gene

  • Yang, Deying;Ren, Yongjun;Fu, Yan;Xie, Yue;Nie, Huaming;Nong, Xiang;Gu, Xiaobin;Wang, Shuxian;Peng, Xuerong;Yang, Guangyou
    • Parasites, Hosts and Diseases
    • /
    • v.51 no.4
    • /
    • pp.449-452
    • /
    • 2013
  • Taenia pisiformis is one of the most important parasites of canines and rabbits. T. pisiformis cysticercus (the larval stage) causes severe damage to rabbit breeding, which results in huge economic losses. In this study, the genetic variation of T. pisiformis was determined in Sichuan Province, China. Fragments of the mitochondrial cytochrome b (cytb) (922 bp) gene were amplified in 53 isolates from 8 regions of T. pisiformis. Overall, 12 haplotypes were found in these 53 cytb sequences. Molecular genetic variations showed 98.4% genetic variation derived from intra-region. $F_{ST}$ and Nm values suggested that 53 isolates were not genetically differentiated and had low levels of genetic diversity. Neutrality indices of the cytb sequences showed the evolution of T. pisiformis followed a neutral mode. Phylogenetic analysis revealed no correlation between phylogeny and geographic distribution. These findings indicate that 53 isolates of T. pisiformis keep a low genetic variation, which provide useful knowledge for monitoring changes in parasite populations for future control strategies.