• Journal of Ginseng Research
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• 제31권3호
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• pp.117-126
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• 2007

Ginseng, the root of Panax species, a well-known herbal medicine has been used as a traditional medicine for thousands of years and is now a popular and worldwide used natural medicine. Ginseng has been used primarily as a tonic to invigorate weak bodies to help the restoration of homeostasis in a wide range of pathological conditions such as cardiovascular diseases, cancer, immune deficiency and hepatotoxicity. Although conclusive clinical data in humans is still missing, recent research results have suggested that some of the active ingredients ginseng exert beneficial effects on central nervous system (CNS) disorders and neurodegenerative diseases, suggesting it could be used in treatment of psychotic disorders. Data from neural cell cultures and animal studies contribute to the understanding of these mechanisms that involve inhibitory effects on stress-induced corticosterone level increasing and modulating of neurontransmitters, reducing $Ca^{2+}$ over-influx, scavenging of free radicals and counteracting excitotoxicity. In this review, we focused on recently reported medicinal effects of ginseng and summarized the possibility of its applications on psychotic disorders.

• Korean Circulation Journal
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• 제53권5호
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• pp.273-293
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• 2023

Pulsed field ablation (PFA) was recently rediscovered as an emerging treatment modality for the ablation of cardiac arrhythmias. Ultra-short high voltage pulses are leading to irreversible electroporation of cardiac cells subsequently resulting in cell death. Current literature of PFA for pulmonary vein isolation (PVI) consistently reported excellent acute and long-term efficacy along with a very low adverse event rate. The undeniable benefit of the novel ablation technique is that cardiac cells are more susceptible to electrical fields whereas surrounding structures such as the pulmonary veins, the phrenic nerve or the esophagus are not, or if at all, minimally affected, which results in a favorable safety profile that is expected to be superior to the current standard of care without compromising efficacy. Nevertheless, the exact mechanisms of electroporation are not yet entirely understood on a cellular basis and pulsed electrical field protocols of different manufactures are not comparable among one another and require their own validation for each indication. Importantly, randomized controlled trials and comparative data to current standard of care modalities, such as radiofrequency- or cryoballoon ablation, are still missing. This review focuses on the "pearls" and "pitfalls" of PFA, a technology that has the potential to become the future leading energy source for PVI and beyond.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8145-8147
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• 2016

Background: Atypical squamous cells of undetermined significance (ASCUS) feature a wide variety of cervical cells, including benign and malignant examples. The management of ASCUS is complicated. Guidelines for office gynecology in Japan recommend performing a high-risk human papillomavirus (HPV) test as a rule. The guidelines also recommend repeat cervical cytology after 6 and 12 months, or immediate colposcopy. The purpose of this study was to determine the clinical significance of ASCUS. Materials and Methods: Between January 2012 and December 2014, a total of 162 patients underwent cervical conization for cervical intraepithelial neoplasia grade 3 (CIN3), carcinoma in situ, squamous cell carcinoma, microinvasive squamous cell carcinoma, and adenocarcinoma in situ at our hospital. The results of cervical cytology prior to conization, the pathology after conization, and high-risk HPV testing were obtained from clinical records and analyzed retrospectively. Results: Based on cervical cytology, 31 (19.1%) of 162 patients were primarily diagnosed with ASCUS. Among these, 25 (80.6%) were positive for high-risk HPV, and the test results of the remaining 6 patients (19.4%) were uncertain. In the final pathological diagnosis after conization, 27 (87.1%) and 4 patients (12.9%) were diagnosed with CIN3 and carcinoma in situ, respectively. Conclusions: Although ASCUS is known as a low-risk abnormal cervical cytology, approximately 20% of patients who underwent cervical conization had ASCUS. The relationship between the cervical cytology of ASCUS and the final pathological results for CIN3 or invasive carcinoma should be investigated statistically. In cases of ASCUS, we recommend HPV tests or colposcopic examination rather than cytological follow-up, because of the risk of missing CIN3 or more advanced disease.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2000년도 International Symposium on Bioinformatics
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• pp.56-58
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• 2000

Post-genomics may be defined in different ways depending on how one views the challenges after the genome. A popular view is to follow the concept of the central dogma in molecular biology, namely from genome to transcriptome to proteome. Projects are going on to analyze gene expression profiles both at the mRNA and protein levels and to catalog protein 3D structure families, which will no doubt help the understanding of information in the genome. However complete, such catalogs of genes, RNAs, and proteins only tell us about the building blocks of life. They do not tell us much about the wiring (interaction) of building blocks, which is essential for uncovering systemic functional behaviors of the cell or the organism. Thus, an alternative view of post-genomics is to go up from the molecular level to the cellular level, and to understand, what I call, the "interactome"or a complete picture of molecular interactions in the cell. KEGG (http://www.genome.ad.jp/kegg/) is our attempt to computerize current knowledge on various cellular processes as a collection of "generalized"protein-protein interaction networks, to develop new graph-based algorithms for predicting such networks from the genome information, and to actually reconstruct the interactomes for all the completely sequenced genomes and some partial genomes. During the reconstruction process, it becomes readily apparent that certain pathways and molecular complexes are present or absent in each organism, indicating modular structures of the interactome. In addition, the reconstruction uncovers missing components in an otherwise complete pathway or complex, which may result from misannotation of the genome or misrepresentation of the KEGG pathway. When combined with additional experimental data on protein-protein interactions, such as by yeast two-hybrid systems, the reconstruction possibly uncovers unknown partners for a particular pathway or complex. Thus, the reconstruction is tightly coupled with the annotation of individual genes, which is maintained in the GENES database in KEGG. We are also trying to expand our literature surrey to include in the GENES database most up-to-date information about gene functions.

• Communications for Statistical Applications and Methods
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• 제16권1호
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• pp.103-113
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• 2009

표본조사에서 무응답은 비 표본추출오차를 발생시키는 중요한 원인 중 하나이다. 단위무응답이 발생하는 경우 무응답에 의한 편향을 줄이는 동시에 추정의 정도를 향상시키기 위해 단위무응답 조정층을 구성해 무응답 가중치 조정을 하는 것이 일반적이다. 본 연구에서는 무응답 조정층 구성과 관련된 기존의 이론들을 정리하고 어업총조사 자료를 이용한 실증적인 모의실험을 통해 효과적으로 무응답 조정층을 구성하는 방안에 대해 살펴본다. 모의실험결과 응답성향에 따른 조정층 구성보다는 예측평균을 기준으로 한 조정층 구성이 효율성 측면에서 효과적인 것으로 나타났으며, 아울러 다른 관심변수에도 적용될 수 있는 로버스트한 조정층 구성을 위해서는 예측평균만을 고려하는 것보다 응답성향과 예측평균을 모두 고려한 조정층 구성방법이 효과적인 것으로 나타났다. 한편 무응답 조정을 위한 응답률 산출에 있어서 설계가중치의 적용 필요성에 대해 살펴본 결과 설계가중치 적용 여부는 추정결과에 거의 영향을 주지 않는다는 사실을 확인할 수 있었다.

• Journal of Ginseng Research
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• 제45권4호
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• pp.473-481
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• 2021

Background: Mitochondrial dysfunction is one of the significant reasons for Alzheimer's disease (AD). Ginsenosides, natural molecules extracted from Panax ginseng, have been demonstrated to exert essential neuroprotective functions, which can ascribe to its anti-oxidative effect, enhancing central metabolism and improving mitochondrial function. However, a comprehensive analysis of cellular mitochondrial bioenergetics after ginsenoside treatment under Aβ-oxidative stress is missing. Methods: The antioxidant activities of ginsenoside Rb₁, Rd, Re, Rg₁ were compared by measuring the cell survival and reactive oxygen species (ROS) formation. Next, the protective effects of ginsenosides of mitochondrial bioenergetics were examined by measuring oxygen consumption rate (OCR) in PC12 cells under Aβ-oxidative stress with an extracellular flux analyzer. Meanwhile, mitochondrial membrane potential (MMP) and mitochondrial dynamics were evaluated by confocal laser scanning microscopy. Results: Ginsenoside Rg₁ possessed the strongest anti-oxidative property, and which therefore provided the best protective function to PC12 cells under the Aβ oxidative stress by increasing ATP production to 3 folds, spare capacity to 2 folds, maximal respiration to 2 folds and non-mitochondrial respiration to 1.5 folds, as compared to Aβ cell model. Furthermore, ginsenoside Rg1 enhanced MMP and mitochondrial interconnectivity, and simultaneously reduced mitochondrial circularity. Conclusion: In the present study, these results demonstrated that ginsenoside Rg₁ could be the best natural compound, as compared with other ginsenosides, by modulating the OCR of cultured PC12 cells during oxidative phosphorylation, in regulating MMP and in improving mitochondria dynamics under Aβ-induced oxidative stress.

• 대한전자공학회논문지SD
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• 제43권11호
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• pp.26-36
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• 2006

본 논문에서는 화소간의 상관관계를 이용한 CCD/CMOS 이미지 센서용 효율적인 색 보간 기법을 제안한다. 최근 각광받고 있는 CCD/CMOS 이미지 센서는 컬러 필터 배열(Color Filter Array)을 사용하기 때문에, 각 화소는 컬러 영상을 만들기 위한 3가지 색 채널 중 한 가지 채널만 갖고 있게 된다. 따라서 컬러 영상을 만들기 위해서는 색 보간 구조가 필요하다. 최근 제안되는 색 보간 기법은 보간된 영상의 품질 향상에만 주력하고 있는데 반해, 본 논문에서는 낮은 복잡도를 갖으면서 잘못된 색을 최소화하기 위한 방법을 제안한다. 제안된 색 보간 기법에서는 인접한 화소간의 상관관계를 이용하여, 현재 화소의 방향성을 결정할 때 이웃 화소의 방향성 정보를 이용하였다. 기존의 방향성을 고려한 색 보간 기법에 제안된 기법을 적용한 결과, 알고리즘의 종류에 따라 PSNR이 $0.09{\sim}0.47dB$ 향상되었고, 대부분의 잘못된 색(False color)을 최소화함으로써 색 보간된 컬러영상의 품질이 향상되었다. 제안된 색 보간 기법은 Verilog HDL 및 FPGA를 이용하여 실시간으로 구현 검증되었다. 0.25um CMOS 표준 셀 라이브러리를 이용하여 합성하였을 때, 총 게이트 수는 12K개였으며 5개의 라인 메모리가 사용되었다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제24권2호
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• pp.173-186
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• 2021

Purpose: Biliary atresia (BA) is a disease that manifests as jaundice after birth and leads to progressive destruction of the ductal system in the liver. The aim of this study was to investigate histopathological changes and immunohistochemically examine the expression of glial cell line-derived neurotrophic factor (GDNF), synaptophysin, and S-100 protein in the gallbladder of BA patients. Methods: The study included a BA group of 29 patients and a control group of 41 children with cholecystectomy. Gallbladder tissue removed during surgery was obtained and examined immunohistochemically and histopathologically. Tissue samples of both groups were immunohistochemically assessed in terms of GDNF, S-100 protein, and synaptophysin expression. Expression was classified as present or absent. Inflammatory activity assessment with hematoxylin and eosin staining and fibrosis assessment with Masson's trichrome staining were performed for tissue sample sections of both groups. Results: Ganglion cells were not present in gallbladder tissue samples of the BA group. Immunohistochemically, GDNF, synaptophysin, and S-100 expression was not detected in the BA group. Histopathological examination revealed more frequent fibrosis and slightly higher inflammatory activity in the BA than in the control group. Conclusion: We speculate that GDNF expression will no longer continue in this region, when the damage caused by inflammation of the extrahepatic bile ducts reaches a critical threshold. The study's findings may represent a missing link in the chain of events forming the etiology of BA and may be helpful in its diagnosis.

• 응용통계연구
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• 제14권1호
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• pp.39-57
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• 2001

범주형 자료에서 오분류는 자료를 수집하는 과정에서 발생될 수 있다. 오분류되어 있는 자료를 정확한 자료로 간주하여 분석한다면 추정결과에 편의가 발생하고 검정력이 약화되는 결과를 초래하게 되며, 정확하게 분류된 자료를 오분류하고 판단한다면 오분류의 수정을 위해 불필요한 비용과 시간을 낭비해야 할 것이다. 따라서 정확하게 분류된 표본인지 오분류된 표본인지를 판정하는 것은 자료를 분석하기 전에 이루어져야할 매우 중요한 과정이다. 본 논문은 I$\times$J 분할표로 주어지는 범주형 자료에서 두 변수 중 하나의 변수에서만 오분류가 발생되는 경우에 오분류 여부를 검정하기 위해서 오분류 가능성이 없는 변수에 대한 주변합은 고정시키고, 오분류 여부를 가능성이 있는 변수의 주변합을 Sebastiani와 Ramoni(1997)가 제안한 Bound와 외부정보로 표현되는 Collapse의 개념, 그리고 베이지안 방법을 확장하여 자료에 적합한 모형과 사전정보를 고려한 사전모수를 다양하게 설정하면서 재분류하는 연구를 하였다. 오분류에 대한 정보를 얻기 위해서 Tenenbein(1970)에 의해 연구된 이중추출법을 이용하여 오분류 검정을 위한 새로운 통계량을 제안하였으며, 제안된 오분류 검정통계량에 관한 분포를 다양한 모의실험을 통하여 연구하였다.

• 대한환경공학회지
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• 제33권3호
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• pp.191-195
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• 2011

본 연구의 목적은 낮은 전단흐름조건에서 편모 운동성이 박테리아의 거동 특성에 미치는 영향을 파악하는데 있다. 대다수의 미생물은 편모를 이용하여 수용액 내에서 운동할 수 있는 능력을 가지고 있으며, 이러한 운동성은 수계나 수처리 시스템에서 미생물의 거동에 있어서 중요한 역할을 한다. 그러나 현재까지 병원성 미생물의 이동 현상과 관련된 연구에서 편모에 의한 운동성은 거의 고려되지 않고 있는 실정이다. 본 연구에서는 미세유체장치를 이용하여 전단흐름이 낮은 조건에서 E. coli의 거동 특성을 파악하고자 하였다. 실험을 통하여 유속이 작은 경우에 E. coli는 포물선의 형태의 궤적들을 그리며 이동하는 것을 알 수 있었으며, 벽면 근처에서는 상류로 헤엄쳐 올라간다는 것을 파악하였다. 또한 유속과 종횡비(aspect ratio)에 따른 궤적의 변화를 분석하였는데, 유속이 작을수록 포물선 형태의 궤적을 그리게 되며, 길이가 짧을수록 보다 작은 회전 반경을 그리며 운동하는 것을 관찰할 수 있었다.