• 환경생물
• /
• 제25권4호
• /
• pp.313-318
• /
• 2007

본 연구는 2003년 8월에 통영 바다목장해역 5개 정점 표층수에서 페놀류, 9개의 폴리클로리네이티드비페닐(PCB) 및 유기주석화합물(TBT)의 농도와 10개 정점에서 수은 및 크롬(VI), 카드뮴, 납의 중금속 농도를 해양 환경공정시험방법(해양수산부 2002) 또는 이 방법과 내분비계 장애물질의 측정 분석방법(국립환경연구원 1999)을 일부 변경하여 분석하였다. 환경정책기본법시행령(대통령령 제18108호: 별표 1, 3. 수질, 라. 해역)에 의하면 페놀류: 5 ppb, PCBs: 0.5 ppb, Hg: 0.5 ppb, Cr (VI): 50 ppb, Cd: 10 ppb 및 Pb: 50 ppb이고 유기주석화합물의 기준은 나타나 있지 않다. 본 해역에서는 페놀류: 1.6$\sim$2.8 ppb, PCBs: 검출되지 않음, Hg: <0.1 ppb, Cr (VI): 0.01$\sim$5.32 ppb, Cd: 미 검출$\sim$0.41 ppb 그리고 Pb: 0.43$\sim$2.60 ppb로써 사람의 건강보호 기준에 적합한 해수로 판단되었다. 유기주석화합물(TBT) 중 Sn의 농도는 미 검출$\sim$0.0273 ppb로써 1998년, Shim et al. (1998)에 의하여 조사된 진해만의 해수 중 TBT의 농도: 8$\sim$35 ng Sn $L^{-1}$ (즉 Sn:0.008$\sim$0.035 ppb)와 유사한 농도였다. 따라서 본 연구에서 조사된 통영 바다목장의 해수는 사람의 건강 기준에 적합한 수질로 판명되었다.

• 한국수정란이식학회:학술대회논문집
• /
• 한국수정란이식학회 2002년도 국제심포지엄
• /
• pp.98-98
• /
• 2002

Recruitment of primordial follicles(PMF) is crucial for female fertility. however, factors and mechanisms that regulate this process is poorly understood. The present study was conducted to obtain an inclusive view of the gene expression and to identify novel factors and their pathways of regulating PMF arrest and/or growth initiation. Ovaries from one-day neonatal(consists of oocyte and PMF) and five-day old(consists of PMF and primary follicles, PRIF) mice were collected, either total RNA or mRNA was isolated, and suppression subtractive hybridization(SSH) was used to isolate and clone genes that differentially expressed in day 1 and day 5 ovaries. Confirmation that some of these genes are differentially expressed in PMF and/or in PRIF was accomplished by using laser captured microdissection(LCM), RT-PCR. in situ hybridization(ISH) and/or immunohistochemistry(IHC). In toto, 357 clones were sequenced and analyzed by BLAST and RIKEN program. Sequences of 330 clones significantly matched database entries while 27 clones were novel. Forty-two and 47 different genes were identified as differentially expressed in day 1 and day 5 ovaries, respectively, while 7 genes were expressed in both stages of ovaries. Day 5-subtracted library included several genes known as markers far growing follicles, such as ZP2, MATER, and fetuin. Among the genes with assigned functions, 23.8% was associated with cell cycle/apoptosis regulation, 7.1% with cellular structure, 11.9% with metabolism, 26.2% with signal transduction, and 31.0% with gene/protein expression in day 1; while 10.6%, 17.0%, 23.5%, 25.5%, and 23.4% in day 5, respectively. Genes such as GDF-8, Lats2, Septin2, and Weel were the highly expressed genes in PMF, while HSP84, Laminin2, MATER, MTi7, PTP, and Wrn were highly expressed genes in PRIF. We have successfully discovered list of genes expressed in day 1 and day 5 ovaries and confirmed that some of them are differentially expressed in PMF and/or PRIF. Gene expression profile from the present study would provide insight for the future study on the mechanism(s) involved in primordial-primary follicular transition. This work was Supported by Korean Health 21 RND Project, Ministry of Health and Welfare, Korea (01-PJ10-PG6-01GN13-0002).

• 한국산업보건학회지
• /
• 제20권3호
• /
• pp.168-174
• /
• 2010

South Korea's industrial injuries are decreasing overall in the last 32 years. Nevertheless, the fatal occupational injury rate is still higher than in developed countries. This study was conducted to help prevention strategies of occupational injuries for the Republic of Korea. Fatal occupational injury rates were obtained from "Industrial Accident Analysis"of the Korean Ministry of Labor. Poisson regression was used to assess time trends. Socioeconomic indicators were obtained from the Korea Labor Institute and the Statistics Korea. Fatal occupational injury rates were adjusted by year, and Pearson correlation analysis was used to assess the relationship between the socio-economic indicators and occupational injuries. In 1975, fatal occupational injury rate was 54.8 per 100,000 workers. With somewhat up and down, it was decreased to 21.0 in 2006. An annual rate of change for the years 1975-2006 was - 1.83%, and for the years 2002-2006 was -5.02%. As economic growth rate, paricipation rate for the age less than 25 and hours of work per week or year increased, fatal occupational injury rate also increased. Conversely, as GDP per capita, paricipation rate or employment rate for female, paricipation rate for the age 25 or more, hourly compensation costs for production workers and services output as percent of GDP increased, fatal occupational injury rate decreased. By the development of safety techniques and the adoption of more legislative constraints, developed economy reduce occupational injuries. Conversely, economic growth may raise occupational injuries. Therefore, prevention strategies are needed to manage both of them. We need to make an effort to prevent occupational injuries due to not only sexual differences, but also job differences between male and female. Preventive strategies are needed to consider the characteristics of younger workers. Addition to wage, other appropriate variables for work condition should be considered together. Extending work hours is need to be regulated with systemic methods.

• 한국환경농학회지
• /
• 제32권2호
• /
• pp.142-147
• /
• 2013

국내 유통 농산물의 잔류농약 안전성을 평가하고자 2012년 전국 6개 도시에서 유통되고 있는 과실류인 사과와 복숭아에 대하여 123점의 시료를 수거하여 잔류농약을 분석하였다. 122종의 농약에 대해 GC-ECD, GC-NPD 및 HPLCUVD을 이용한 다성분분석법으로 잔류농약을 분석하였고, 분석 결과 총 농약이 검출된 시료는 관행농산물에서 38점, 저농약농산물에서 6점, GAP농산물에서 1점으로 시료에서 16종의 농약이 검출되어 33.6% 검출률을 나타내었다. 농약 성분별 검출 빈도는 cypermethrin, lufenuron, fenvalerate, bifenthrin, chlorfenapyr, iprodione 순이었다. 농약이 검출된 시료 중 잔류허용기준을 초과하는 시료는 18점으로 14.6% 검출되었으며, 해당 작물에 대한 잔류허용기준이 설정되어 있지 않거나 품목고시 되어있지 않은 농약은 2점의 시료에서 2종의 농약이 검출되어 1.6% 검출률을 보였다. 본 연구에서 검출된 농약이 해당 농산물의 섭취로 인체에 유입될 잔류농약의 일일섭취허용량 대비 일일섭취추정량은 최저 0.033%에서 최고 2.748%로 농산물 섭취에 의한 위해성은 낮은 것으로 나타났다.

• 한국산업보건학회지
• /
• 제1권2호
• /
• pp.181-191
• /
• 1991

The present study determined the overall noise level and the distribution of sound pressure level over audible frequency range of noise produced at various work sites. Work-related noise greater than 80dBA produced from 98 separate work sites at 37 manufacturing companies and machine shops were analysed for the overall sound level (dBA) and frequency distribution. In addition, to determine the possible hearing loss related to work site noise, a hearing test was also conducted on 1,374 workers in these work sites. The results of the study were as follows ; 1. Of the total 98 work sites, 57 work sites(58.2%) produced noise exceeding threshold limit value (${\geq}90dBA$) set by the Ministry 01 Labor. In terms of different manufacturing industries the proportion of work sites which exceeded 90dBA was the highest for the cut-stone products industry with 6/6 work sites and lowest for the commercial printing industry with 1/13 work sites. 2. The percentage of workers who were exposed to noise greater than 90dBA was 19.8% (1,040 workers) 01 the total 5,261 workers. In terms of different industries, cut-stone products industry had the most workers exposed to noise exceeding 90dBA with 82.8%, textile bleaching and dyeing industry was next at 30.6% followed by fabricated metal products industry with 27.9%, plastic products manufacturing industry had the lowest percentage of workers exposed to 90dBA exceeding noise with 4.5%. 3. There was a statistically significant correlation between the frequency of noise-induced hearing loss and the percentage of workers exposed to noise exceeding 90dBA (P<0.05). 4. The frequency analysis of noise produced at the 98 work sites revealed that 44 work sites (44.9%) had the maximum sound pressure level at high-frequencies greater than 2KHz. In addition, significantly higher sound pressure level was detected at the high-frequencies at 90dBA exceeding work sites as compared to below 90dBA work sites (P<0.01). 5. The differences in sound level meter's A-and C-weighted sound pressure levels were analysed by frequencies. Of the 28 work sites which showed 0-1 dB difference in the two weighted sound levels, 20 work sites (71.4%) had significantly higher sound pressure levels at high-frequencies greater than 2KHz (P<0.01). Furthermore, there was a tendency for higher sound pressure levels to occur in the high-frequency range as the differences in the two weighted sound levels decreased.

• Journal of Periodontal and Implant Science
• /
• 제31권3호
• /
• pp.565-571
• /
• 2001

The purpose of this study is to determine if a relationship exists among osteoporosis, alveolar bone density and periodontal disease in postmenopausal osteoporotic women and postmenopausal healthy women. Twenty-two women were evaluated for this study. They were attending the postmenopausal clinic, Seoul National University Hospital and generally healthy except osteoporosis. They had experienced menopause not less than one year when we began to examine them. Bone densities of lumbar area(L2-L4) was determined by DEXA(LUNAR-expert Co,. U.S.A). We diagnosed osteoporosis when T-score was below -2.5 and healthy state when T-score was over -1. Osteoporotic(10 female), not hormone-treated group and healthy control group(12 female) were asked for their age, menopausal age, menopausal period and the number of remaining teeth and examined clinically for plaque index(PI), gingival index(GI), clinical attachment loss(CAL) on their 6 Ramfjord index teeth. Intraoral radiographs were taken in maxillary anterior zone. All films were equally exposed and developed. Each films was digitized and analysed using image processing software, Scion image. Alveolar bone regions of interest were selected and Intensity of each pixel was quantized in the array ranging from 0(white) to 255(black). The two groups were comparable with respect age, menopausal age, menopausal period and number of remaining teeth. The osteoporotic women had significantly lower alveolar bone density than controls in maxilla. But no significant difference was found with respect clinical attachment loss, plaque index and gingival index. Supported by the Ministry of Public Health and Welfare, Korea (HMP-00-CH-10-0009).

• 한국토양비료학회지
• /
• 제43권5호
• /
• pp.674-683
• /
• 2010

본 연구에서는 폐광산 주변 농경지 용도의 경작 적합성을 판단하고자 토양의 위해성을 정량적으로 측정할 수 있는 과학적 방법인 한국, 미국 및 영국의 위해성평가를 이용하였다. 예비위해성평가를 통하여 선택한 DM, MG 및 KS광산을 위해성평가 대상 지역으로 선택하여 토양 및 농작물 시료를 채취하였다. 이후 토양과 농작물 시료 중 중금속 분석을 통하여 위해성 평가 및 농경지 적합성 판단을 위한 변수를 확보하였다. 토양 중 중금속 분석결과, 환경부 보고서에 수록된 수치보다 토양 중 중금속의 함량이 낮은 것을 확인하였으며, 대상 광산지역 모두에서 토양 중 Cd의 함량이 토양 환경보전법의 1 지역 토양오염우려기준을 초과하고 있는 것을 확인 할 수 있었다. S3와 S6지역은 As함량이 각각 1 지역의 대책기준과 우려기준을 초과하였다. 농작물 내 중금속 농도는 작물의 중금속 한계 농도를 근거로 DM광산주변에서 재배되고 있는 고추, 콩, 옥수수에서 Zn의 경우에만 오염식물의 범위로 판단되었으며, 이 외의 중금속 함량은 Kabata-pendias and Pendias의 일반적으로 오염되지 않은 식물의 배경농도 범위에 비교하여 안전한 수준을 보였다. 위해성평가 결과에 따르면 한국과 미국의 위해 성평가 모델의 경우, MG광산과 KS광산에서 농작물 내 Pb에 의한 인체 위해성이 나타날 것으로 예상되었고, 영국의 모델에서는 조사 전 지역에서 농작물의 Pb에 의한 독성이 나타날 것으로 예상되었다. 이 후 위해성평가 모델을 통한 토양 중 중금속의 오염기준을 한국의 정화목표치과 영국의 토양지침값으로 도출하고, 현재 토양 중 중금속 농도와 비교하였다. 그 결과 현재 토양 중 중금속의 농도가 영국의 토양지침값을 초과하여 MG광산, DM광산 및 KS광산지역에서 농경지 용도로 토지를 사용하는 데 문제가 있을 것으로 판단되었고, 오염물질에 대한 대책이 필요함을 알 수 있었다.

• 한국식품위생안전성학회지
• /
• 제28권2호
• /
• pp.115-123
• /
• 2013

식품안전관리는 과학적 근거에 따라 진행되어야 한다. FAO/WHO에서는 식품안전관리 4대 원칙 중 하나로 위해성 분석을 제시하고 있으며, WTO에서는 위해성 평가에 입각한 경우에 한하여 자체적인 안전기준을 인정하고 있다. 과학적 분석 없이는 식중독 발생의 원인을 추적 제거함으로써 재발을 막는 것 또한 불가능하다. 연구개발은 과학적 근거를 생산하는 중요한 역할을 한다. 정부의 식품안전 연구개발은 11개 정부기관에서 40개가 넘는 사업을 통해 진행되고 있다. 그렇다보니 정확한 통계 자료를 확보하기 어렵다. 본 연구에서는 사업명, 과학기술표준분류, 키워드라는 3가지 기준에 따라 국가과학기술지식정보서비스(NTIS)에서 제공하는 데이터베이스를 활용해 2008년부터 2010년까지 진행된 식품안전 연구과제를 추출하였다. 분석 결과, 식품의약품안전청과 농림수산식품부 및 농촌진흥청의 연구사업이 중요한 비중을 차지하고 있다. 위해성 평가를 위한 기초자료 확보 등을 위해서는 현재보다 1년 이상 다년도 과제의 비중을 높일 필요가 있다. 또한 SCI 논문을 투고할 수 있는 수준으로 연구의 깊이를 높일 필요도 있다. 노로바이러스 등 생물학적 위해에 대한 연구비중을 높이는 방향으로 연구 포트폴리오의 조정이 필요하다. 이를 위해서는 식품안전 관련 사업의 통합 집중화할 필요가 있다. 별다른 차이가 없음에도 부처가 다르다보니 별도로 설정하고 있는 식품안전관리와 농축산물 위생/품질관리로 이원화된 과학기술표준분류의 통합도 필요하다.

• 사물인터넷융복합논문지
• /
• 제8권2호
• /
• pp.13-24
• /
• 2022

본 연구는 우리나라에서 매년 증가하고 있는 노인장기요양기관의 부당청구 맥락과 부당청구 예방을 위한 대책들이 어떠한지를 탐색하기 위해서 언론기사를 활용한 텍스트 마이닝 분석을 실시하였다. 기사는 뉴스 빅테이터 분석 시스템인 빅카인즈에서 수집하였고, 수집기간은 노인장기요양보험이 시행된 2008년 7월부터 2022년 2월 28일까지로 약 15년간이다. 이 기간 동안 '노인요양+부당청구', '장기요양+부당청구', 등의 키워드로 총 2,627개의 기사가 수집되었고, 이중 중복된 기사를 제외한 총 946개가 선정되었다. 본 연구의 텍스트마이닝 분석결과로 첫째, 모든 구간(2008.7.1-2022.2.28)에서 가장 높은 빈도로 언급된 상위 10위 키워드는 노인장기요양기관, 부당청구, 국민건강보험공단, 노인장기요양보험, 장기요양급여(비용), 노인요양시설, 보건복지부, 노인, 신고, 포상금(지급)의 순으로 나타났다. 둘째, N-gram 분석결과 장기요양급여(비용)과 부당청구, 부당청구와 노인장기요양기관, 허위와 부당청구, 신고와 포상금(지급), 노인장기요양기관과 신고 등의 순으로 나타났다. 셋째, TF-IDF 분석은 빈도분석의 결과와 유사하게 나타났지만, 신고, 포상금(지급), 증가 등은 순위가 상승하였다. 상기 분석결과를 바탕으로 노인장기요양기관 부당청구 예방을 위한 방향성을 제시하였다.

• 한국산업보건학회지
• /
• 제23권3호
• /
• pp.212-221
• /
• 2013

Objectives: Asbestos fibers are accumulated in negative pressure unit (NPU) or vacuum cleaner in asbestos removal. Failure of operation or poor use performance of the NPU or vacuum cleaner causes asbestos fibers to spread in the air. Asbestos contractors should have an NPU fitted with a HEPA , vacuum cleaner, decontamination area, respirators, wetting equipment, and differential pressure manometer to register with the Ministry of Employment and Labor. There should be performance criteria of equipment used in asbestos removal. But there are none such criteria used in asbestos removal. This study was performed to investigate international or national performance criteria of equipment used in asbestos removal. Methods: Regulations, approved code of practice, guidance and national standards of the UK, USA and Korea were reviewed. A survey was done to investigate the requirements for equipment used in asbestos removal in Korea. Results: Air flow of NPUs used in Korea usually covers from$500m^3/h$ to over $3000m^3/h$. Some requirements for NPUs used in Korea were missing compared to the requirements of British standards for NPUs. All NPUs have different missing requirements. The UK also has separate British standard for leak tests for NPUs. Highly hazardous class vacuum cleaners should be used in asbestos removal in the UK. It has national standards on the operation of vacuum cleaners used in asbestos removal. There is only a certification system for less than 2.5kw rated voltage vacuum cleaners for home use in Korea. Powered (-assisted) respirators with mask are recommended in asbestos removal. Type 5 coveralls should be used in asbestos removal in the UK. There are international standards for requirements and leak tests of type 5 coveralls. A manometer for measuring differential pressure is needed in asbestos removal. The manometer's measuring range should cover less than ${\pm}125$ Pa and the gradation of manometer should be less than 2.5 Pa. The definition of decontamination area should be corrected. Installation of airlock and minimum area should be considered in decontamination area. Conclusions: Equipment should be used in asbestos removal proper performance and no leaks. There should be a certification system for equipment used in asbestos removal. This study can help to a certification system for equipment used in asbestos removal in Korea.