• International Journal of Advanced Culture Technology
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• 제12권1호
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• pp.281-286
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• 2024

With the commercialization and full-timeization of the livestock industry, civil complaints continue to increase, and the livestock industry is facing a crisis due to social problems such as odor caused by livestock manure, soil pollution, water pollution, and environmental pollution. In order to increase productivity in livestock farms, the amount of livestock manure generated is increasing due to excessive use of protein feed and high-density breeding environment, and complaints such as odor and water pollution due to management problems are increasing rapidly. Livestock odor has emerged as a serious social problem, and due to growing complaints, conflicts between the Ministry of Agriculture and Forestry and the Ministry of Environment are even causing the livestock industry to lower its status. There is an urgent need for solutions to identify problems in the livestock industry and improve policies. This study aims to develop a " Abelmoschus Manihot Jinhuakui " brand that can improve the intestinal environment of livestock, reduce odors caused by livestock excrement, and improve the productivity of livestock farms in order to improve the increasingly serious odor problem in livestock sites. For Jeju livestock farms, which place more importance on the environment by securing tourists, eco-friendly feed additives were applied to the experiment, the results were derived, and the focus was on solving fundamental problems of odor generation through the development and packaging of feed additive brands. We aim to fundamentally solve the odor problem of domestic livestock farms, secure eco-friendly livestock farms, and contribute to reducing livestock odors and increasing productivity through research results that reduce ammonia levels in each livestock farm.

• 창의정보문화연구
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• 제6권3호
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• pp.149-158
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• 2020

코로나19로 인해 초중등학교 및 고등교육기관에서의 교수학습 방법의 급진적인 변화를 경험하였고, 교육부는 원격교육상황에서 교수학습의 질을 담보하고, 학습 참여를 촉진하기 위한 다양한 시도와 지원이 지속되고 있다. 이 연구에서는 포스트 코로나 시대 대비 교육부 지원 정책에 대해 살펴보고, 원격교육에 대한 교수자의 경험을 집중면담을 통해 조사하였다. 이를 토대로 학습자의 학습 참여 촉진을 위한 방안을 모색하였다. 실시간 원격교육에서 학습 참여를 지원하는 프로그램 활용방안으로, 첫째, 학습자의 적정 학습 집중 기간에 대한 고려, 둘째, 학습활동 중 예기치 못한 문제 상황 발생 대처 방안, 협업 도구로 소회의실 및 채팅 활용방안에 대해 제시하였다.

• 한국식생활문화학회지
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• 제32권3호
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• pp.244-257
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• 2017

Nutrition labeling can provide information in order for people to select products suitable for their own health, and sodium content labeling for processed foods is important since sodium is one of the main causes of cardiac diseases. This study had carried out to propose the desirable sodium content labeling by conducting surveys on the awareness of sodium content in processed foods, understanding of sodium content labeling, and requirements for new sodium comparative claims. The survey period was from 12th of September, 2016 through the 24th, during which a self-administered questionnaire survey was given to 1,003 persons through demographic quota sampling by age and region. As a result of the survey, 66.0% of respondents assuring nutrition labeling answered they check sodium content labeling, whereas 83.2% were aware of excessive intake of sodium having a negative effect on health. Exactly 49.9% of respondents answered that the current system for nutrition labeling on processed foods does not help one to understand the content of sodium, whereas 72.9% answered they wanted to compare sodium contents with those of other products when buying or taking processed foods. As 92.5% cited the importance of sodium comparative claims made by processed foods, preparation of a new system for food labeling should be considered by which consumers can easily compare sodium contents with those of other similar products.

• 문화기술의 융합
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• 제8권6호
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• pp.569-580
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• 2022

현재, 교육부 장관이 교육부 중심으로 유보통합추진하는 것을 공식 발표하였다. 유보통합의 핵심은 유치원과 어린이집의 통합으로, 구체적인 모델(안)을 개발할 필요가 있어 본 연구를 수행하였다. 유보통합 논의 이후 유아학교를 중심으로 논의가 되어 왔고, 구체적인 사안에서 의견 차이가 있었다. 이에 본 연구는 교육부 중심으로 유치원과 어린이집이 통합된 이후 유아학교 모델(안)의 단계별 방향을 제안하고자 수행하였다. 연구문제는 교육부 중심 0-5세 유아학교 모델(안)은 어떠한지 알아보는 것이다. 본 연구에서는 유보통합이 실현된 이후의 설립유형별 유아교육기관 통합 방안을 제안하였다. 본 연구의 결론으로 첫째, 국가책임 단계별 0-5세 유아학교 모델(안)이 요구된다. 둘째, 다양한 설립유형별 특성을 반영한 세부적인 유아학교 모델(안)이 개발되어야 한다. 셋째, 유아학교 체제시 교원양성체계 개편, 방과후과정 운영 모델, 교육청 및 지자체 통합 방안 등의 구체적인 방안이 제시되어야 한다.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.116-120
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• 2004

The medicinal properties of Ganoderma lucidum have been recognized in China for many centuries. Active pharmaceutical components include triterpenes. To elucidate the molecular regulation of triterpene biosynthesis in this mushroom, a 57-base pair DNA fragment encoding the fourth conserved domain SQ-4 (SMGLFLQKTNIIRDYNEDL) of squalene synthase was synthesized and cloned into the expression vector pET-32a(＋). The recombinant fusion protein induced by IPTG (isopropyl-$\beta$-D-thiogalactopyranoside) was overexpressed in the Escherichia coli. Using the purified recombinant fusion protein of 20.9 kDa, a specific polyclonal antibody was obtained from immunized rabbit. Expression of squalene synthase at different development stages of Ganoderma lucidum was analyzed.

• Journal of Microbiology and Biotechnology
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• 제23권12호
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• pp.1673-1682
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• 2013

Citrobacter sp. is a cause of significant opportunistic nosocomial infection and is frequently found in human and animal feces, soil, and sewage water, and even in industrial waste or putrefaction. Biofilm formation is an important virulence trait of Citrobacter sp. pathogens but the process and characteristics of this formation are unclear. Therefore, we employed in vitro assays to study the nutritional and environmental parameters that might influence biofilm formation of C. werkmanii BF-6 using 96-well microtiter plates. In addition, we detected the relative transcript levels of biofilm formation genes by RT-PCR. Our results indicated that the capacity of C. werkmanii BF-6 to form biofilms was affected by culture temperature, media, time, pH, and the osmotic agents glucose, sucrose, NaCl, and KCl. Confocal laser scanning microscopy results illustrated that the structure of biofilms and extracellular polysaccharide was influenced by 100 mM NaCl or 100 mM KCl. In addition, nine biofilm formation genes (bsmA, bssR, bssS, csgD, csgE, csgF, mrkA, mrkB, and mrkE) were found to contribute to planktonic and biofilm growth. Our data suggest that biofilm formation by C. werkmanii BF-6 is affected by nutritional and environmental factors, which could pave the way to the prevention and elimination of biofilm formation using proper strategies.

• 대한수의학회지
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• 제58권4호
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• pp.177-182
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• 2018

Canine adenovirus type 2 (CAV-2) infection results in significant respiratory illness in dogs. Isolating and culturing CAV-2 allows for investigations into its pathogenesis and the development of vaccines and diagnostic assays. In this study, we successfully isolated a virus from a naturally infected dog in Gyeonggi-do, Korea. The virus was propagated in Madin-Darby canine kidney (MDCK) and Vero cells and showed a specific cytopathic morphology that appeared similar to a bunch of grapes. The virus was first confirmed as CAV-2 based on these cytopathic effects, an immunofluorescence assay, hemagglutination assay, and electron microscopy. The viral titer of the isolate designated APQA1601 reached $10^{6.5}$ 50% tissue culture infections dose per mL in MDCK cells and exhibited no hemagglutination units with erythrocytes from guinea pig. The virus was also confirmed by polymerase chain reaction and next-generation sequencing. The APQA1601 strain had the highest similarity (~99.9%) with the Toronto A26/61 strain, which was isolated in Canada in 1976 when the nucleotide sequences of the full genome of the APQA1601 strain were compared with those of other CAV strains. Isolating CAV-2 will help elucidate the biological properties of CAV-2 circulating in Korean dogs.

• Asian-Australasian Journal of Animal Sciences
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• 제29권10호
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• pp.1500-1507
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• 2016

Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause "double-muscling" trait in animals. In order to block the inhibiting effect of MSTN on muscle growth, we transferred zinc-finger nucleases (ZFN) which targeted sheep MSTN gene into cultured fibroblasts. Gene targeted colonies were isolated from transfected fibroblasts by serial dilution culture and screened by sequencing. Two colonies were identified with mono-allele mutation and one colony with bi-allelic deletion. Further, we introduced the MSTN-ZFN mRNA into sheep embryos by microinjection. Thirteen of thirty-seven parthenogenetic embryos were targeted by ZFN, with the efficiency of 35%. Our work established the technical foundation for generation of MSTN gene editing sheep by somatic cloning and microinjection ZFN into embryos.

• 한국항해항만학회지
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• 제30권4호
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• pp.295-302
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• 2006

This study is to integratedly examine coastal management policy and marine tourism development project for Korean coasts, especially for the South Sea of high development pressure, presenting sustainable tourism development policies for the future. To do so, it is examined central government-level coastal development projects set up by the Ministry of Culture and Tourism, the Ministry of Maritime Affairs and Fisheries and the Ministry cf Construction and Transportation, setting the direction of south coastal management and tourism development at a level of national territory planning. The problems of coastal management first and then the problems of the South Coast Tourism Belt Project are analyzed in order to present political and administrative alternatives. To overcome such problems and make Korea a marine tourism base in East Asia, there is a need for re-recognition of the value of the project and its continuous push through cooperation between central and local governments. Also, under the presupposition of consensus building among local people and the sustainable development of environments, there should be are-recognition that the future cf Korean marine tourism in the 21st-century and the success of an inverted $\pi-axis$ national development depend on the South coastal Tourism Belt Development Project.

• The International Journal of Costume Culture
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• 제13권1호
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• pp.23-25
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• 2010

Archaeological evidence shows that cotton fibres of the genus Gossypium were first used for textile production at Mehrgarh, Pakistan in the Neolithic period ($6^{th}$ millenium BC). Eventually the cotton plant and textiles found their way to Egypt and through there, to Greece. However, the chronological origins of the cultivation and/or use of cotton fibres in textile production in ancient Greece are ambiguous. The main sources of information are ancient written texts and excavated textile finds. Both indicate that the introduction of cotton in Greece can be placed in the mid-first millenium BC. However, it is not clear whether the cultivation of the plant and consequently the production of cotton textiles was established in that period or whether another cotton species was produced and used locally in Greece or whether isolated examples of cotton textiles had been imported in Greece before the cotton plant. A more comprehensive analytical study of surviving textile finds coupled with archaeological research for plant production and cultivation would provide the answer to these questions.