• 대한약침학회지
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• 제15권1호
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• pp.23-28
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• 2012

Objective: This experimental study was designed to investigate the safety and the effectiveness of Samjeong pharmacopuncture solution (SPS) manufactured by using a the lowtemperature extract on process. Methods: To identify the safety and the effectiveness of using SPS as eye drops, we performed applied eye irritation tests on rabbits and antibacterial tests for Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans. The eye irritation test was performed according to the toxicity testing regulation of the Korea Food & Drug Administration (2009. 8. 24, KFDA 2009-116). After SPS had been applied on the left eye of the rabbits, eye irritation in the cornea, iris and conjunctiva was observed on the 1st, 2nd, 3rd, 4th & 7th day. After SPS had been dropped on bacterial species that cause keratitis, the minimum inhibition concentration and the size of the inhibition zone were measured. The anti-bacterial potency was also measured by taking the size of inhibition zone. Results: After SPS had been administered on the left eye of the rabbits, none of nine rabbits were found to show abnormal signs or weight changes. After SPS had been administered on the left eye of the rabbits, no eye irritation in the cornea, iris and conjunctiva was observed on the 1st, 2nd, 3rd, 4th & 7th day. No specific response was detected in MIC for bacterial species Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans after SPS had been applied. Conclusions: This study suggests that SPS is a non-toxic and non-irritant medicine that does not cause any of eye irritation in rabbits, but it has no antibacterial effects on bacterial species that are well known to cause keratitis. These results suggest that more research is required on extracts from herbal medicines for treating keratitis.

• 대한약침학회지
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• 제12권1호
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• pp.21-33
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• 2009

Objective : This experimental study was performed to investigate the safety of Soyeom Pharmacopunture solution manufactured by extraction of alcohol and water. To identify the use of it as eyedrops, the eye irritation test of rabbits and antibacterial test of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans was performed. Methods : 1. The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration(2005. 10. 21, KFDA 2005-60). After Soyeom pharmacopuncture solution was administered in the left eye of the rabbits, eye irritation of the cornea, iris and conjunctiva was observed at 1, 2, 3, 4 & 7day. 2. After administering Soyeom Pharmacopuncture solution on bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, MIC(Minimum Inhibition Concentration) and the size of inhibition zone were measured. Anti-bacterial potency was also measured using the size of inhibition zone. Results : 1. After Soyeom pharmacopuncture solution was administered in the left eye of the rabbits, it was found that none of nine rabbits have abnormal signs and weight changes. 2. After Soyeom pharmacopuncture solution was medicated in the left eye of the rabbits, no eye irritation of the cornea, iris and conjunctiva was observed at 1, 2, 3, 4 & 7day. 3. There was no response to MIC on bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) after Soyeom pharmacopuncture solution was medicated. Conclusions : The present study suggests that Soyeom pharmacopuncture solution is a non-toxic and non-irritant medicine, which does not cause eye irritation in rabbits, but dosen't have anti-bacterial effects on bacterial species which cause Keratitis. These study result recommends that more research on other herbal medicines of eye drop for Keratitis are required.

• 생약학회지
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• 제50권3호
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• pp.191-197
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• 2019

Sepsis, an infectious disease, is a life-threatening condition that arises when the response to infection causes injury to tissues and organs. The purpose of this study was to demonstrate whether ABHC-1 and ABHC-2, two functional extracts from herbal complex, have an anti-bacterial effect against Escherchia coli in vivo, in vitro experimental model. ABHC-1 and ABHC-2 showed the antibacterial activity against the bacteria by paper disc method. The minimum inhibitory concentration (MIC) was measured using alamar blue reagent. The MIC was shown at $60{\mu}g/ml$ from ABHC-1 and $500{\mu}g/ml$ from ABHC-2 against E. coli. We next examined the effect of ABHCs on the production of inflammatory cytokine, such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), which is related to the induction of inflammation, in RAW 264.7 cell. ABHC-1 and ABHC-2 increased $TNF-{\alpha}$ production of RAW 264.7 cell in a dose-dependent manner while two extract decreased $TNF-{\alpha}$ production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell in a dose-dependent manner. At a dose of $1{\times}10^8$ E. coli. i.p., non-treated mice were succumbed, while most of mice treated with ABHC-1 were survived. Therefore, our results suggest that ABHC-1 has anti-bacterial activity and can be a novel therapeutic agent against infectious diseases.

• 한국응용곤충학회지
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• 제24권1호
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• pp.19-24
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• 1985

국내(國內) 주요(主要) 사과산지(産地) 4개처(個處)에서 저장중(貯藏中) 부패과(腐敗果) 및 수확전(收穫前) 과수원(果樹園) 이병과(罹病果)에서 Alternaria mali를 분리(分離)하여 각종(各種) 살균제(殺菌劑)를 가용(加用)한 PSA배지상(培地上)에 배양(培養)하여 균(菌)의 내성(耐性)을 조사(調査)한 결과(結果)는 다음과 같다. 지역별(地域別) 및 계절별(季節別) 균주간(菌株間)에는 약제내성(藥劑耐性) 차이(差異)가 적었으며, 살균제(殺菌劑)의 종류(種類) 및 농도(濃度)에 따른 차이(差異)는 컸다. Chlorothalonil 및 captafol의 균생육(菌生育)에 대(對)한 MIC 는 각각(各各) 100,238 및 $81,000{\mu}g/ml$ 이상(以上)으로서 효과(效果)가 가장 낮았으며 균(菌)의 내성(耐性)이 가장 높았다. lprodione 및 folpet의 MIC는 각각(各各) 3,825 및 $3,000{\mu}g/ml$ 이상(以上)으로 중도(中度)의 효과(效果)를 보였으며 균(菌)의 내성(耐性)도 중도(中度)였다. polyoxin의 MIC는 $900{\mu}g/ml$로서 효과(效果)가 높았으나 이는 권장농도(勸奬濃度)의 9배(倍)로서 균(菌)의 내성(耐性)이 약간(若干) 발생(發生)한 경향(傾向)을 보였다. polydong 및 oxidong 의 MIC 는 각각(各各) 500 및 $1,000{\mu}g/ml$ 이하(以下)로서 효과(效果)가 가장 높았으며 권장농도(勸奬濃度) 이하(以下)에서 균생육(菌生育)을 억제(抑制)하였으므로 현재(現在)로서는 농가(農家) 과수원(果樹園)에서 균(菌)의 내성(耐性)이 가장 낮았다. 공시(供試) 살균제(殺菌劑) 7종(種)에 대(對)한 사과 반점(斑點) 낙엽병균(落葉病菌)의 균사생장(菌絲生長), 포자형성(胞子形成) 및 포자발아(胞子發芽)는 같은 경향(傾向)의 반응(反應)을 보였다.

• 경제분석
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• 제27권3호
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• pp.1-32
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• 2021

본 연구는 통화정책 전달경로 중 은행자본경로와 위험추구경로가 국내은행에도 작동하는지에 대해 실증분석하였다. 이를 위해 확장적 통화정책이 은행의 예대금리차, BIS비율, 위험가중자산비율 및 대출 등에 미치는 영향을 분석하였다. 실증분석은 기존 연구에서 통화정책 영향분석에 많이 사용된 Uhlig (2005)의 부호제약 SVAR(Structural VAR) 모형을 토대로 실시하였다. 본 연구는 실증분석을 통해 국내외 기존 연구 결과와 유사하게 국내은행에서도 은행자본경로 및 위험추구경로가 작동하는 것을 확인하였다. 확장적 통화충격에 대해 국내은행의 예대금리차는 확대되었으며, 자기자본비율은 개선되었고, 이후 총대출이 증가하는 것으로 분석되었다. 이는 은행자본경로가 작동함을 의미한다. 또한, 실질콜금리에 대한 확장적 통화충격에 대해 위험가중자산비율이 증가하는 것으로 분석되었다. 이는 위험추구경로가 작동함을 의미한다. 본 연구의 시사점은 다음과 같다. 확장적 통화정책 지속 시 은행자본경로 작동으로 단기적으로 은행 건전성과 수익성이 개선되는 효과도 있으나, 위험추구경로 작동으로 중장기적으로 은행의 위험추구(risk-taking) 행위가 심화되어 은행의 실질적 건전성에 악영향을 줄 수 있다. 이에 따라 금융당국은 확장적 통화정책 지속 시 편중리스크 발생 및 시스템리스크 증가 등에 대한 선제적인 모니터링 및 감독을 강화할 필요가 있다.

• The Korean Journal of Physiology
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• 제21권2호
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• pp.211-223
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• 1987

Head-down tilt (HDT) at $-6^{\circ}$ has been commonly used as the experimental model in both man and animals to induce the blood shift toward the head or central protion of the body, demonstrating similar physiological effect encountered in the weightlessness in the orbital flight. There are few reports about the physiological response upon the cardiovascular regulatory system or the tolerance to the $(-6^{\circ})$ HDT within a relatively short period less than 1 hour. Therefore, the purpose of this study way to observe the effects of $-6^{\circ}$ HDT on cardiovascular system within 30 minutes and to evaluate early regulatory mechanism for simulated hypogravity. Ten mongrel dogs weighing 8-12 kg were anesthetized with the infusion of 1% ${\alpha}-chloralose$ (100 mg/kg) intravenously, and the postural changes were performed from the supine to the $-6^{\circ}$ head-down Position, then from the head-down to the supine (SUP), and each posture was maintained for 30 minutes. Blood flow $({\dot{Q}})$ through common carotid and femoral arteries were determined by the electromagnetic flowmeter. Mean arterial pressure (MAP), heart rate (HR), respiratory rate , and pH, $P_{O_2}$, $P_{CO_2}$ and hematocrit (Hct) of arterial and venous blood were also measured. The peripheral vascular resistance was calculated by dividing respective MAP values by ${\dot{Q}}$ through both sides of common carotid or femoral arteries. The concentration of plasma epinephrine and norepinephrine was determined by Peuler & Johnson's radioenzymatic method. The results are summarized as follows: In the initial 5 minutes in $-6^{\circ}$ HDT, HR was significantly (p<0.05) increased and MAP slightly decreased. Although ${\dot{Q}}$ and carotid peripheral artery resistance were not significantly changed, ${\dot{Q}}$ through femoral artery was diminished and femoral peripheral artery resistance was elevated. In the SUP, the initial changes of MAP and HR were increased (p<0.05), but those of ${\dot{Q}}$ and peripheral vascular resistance through both common carotid and femoral arteries were not significant. After 10 minutes of each postural change in both HDT and SUP, MAP was maintained almost equal to that of the level of pretilting control. During 60 minutes of both postural changes of HDT and SUP, $P_{O_2}$ and Hct were not changed significantly. However pH tended to increase slowly and $P_{CO_2}$ was gradually decreased. The pH and $P_{CO_2}$ seemed to be related to the increased respiratory rate. Plasma epinephrine concentration was not changed significantly and plasma norepinephrine concentration was slightly decreased in the course of HDT and also at 10 minutes of SUP. However these concentration changes were statistically insignificant. From these results, it may be concluded that the effect of $-6^{\circ}$ HDT for 30 minutes on the cardiovascular system and plasma catecholamine levels of the dog is minimum and it is suggestive that the cardiovascular regulatory mechanism, possibly mediated by so called gravity receptors including baroreceptor and volume receptor, has been properly and adequately operated.

• Journal of Periodontal and Implant Science
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• 제30권3호
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• pp.661-676
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• 2000

Gingivitis and periodontitis are infectious diseases in that microorganisms are the primary extrinsic cause of the diseases. the occurrence of gingivitis has been associated clearly with the presence of microorganisms at the disease site, and the histologic nature of the tissue involved is indicative of an inflammatory response induced by microorganisms. additional evidence for the microbial etiology of periodontal disease is that numerous antimicrobial agents are effective in reducing plaque accumulation and periodontal diseases. the purpose of this article is to analyze the antimicrobial effects of Pulsatilla koreana. Well-dried Pulsatilla koreana purchased from herbs distributor was ground and extracted into methanol(MeOH), ethylacetate(EtoAc), chlorform($CHCl_3$) and Butyl alcohol(BuOH). we have then applied each solution to the bacteria samples(Bacteroides forsythus, Streptococcus mutans, Streptococcus sanguis, Porphylomonas gingivalis, Actinobacillus actinomycetemcomitans, Eikenella corrodens, Prevotella intermedia, Actinomyces viscosus, Prevotella nigrescens , Rothia dentocariosa, Fusobacterium nucleatum, Pseudomonas aeruginosa, Staphylococcus aureus) collected from several organizations. To conduct susceptibility test(Kirby-Bauer method), plate contained each periodontopathic bacteria is spread extracted into methanol(MeOH), ethylacetate(EtoAc), chlorform($CHCl_3$) and Butyl alcohol(BuOH) and to measure the minimum inhibition concentration(MIC) of the bacteria against the solutions to ultimately determine antimicrobial effects of the solutions, insert bacteria sample into $20{\mu\ell}/{m\ell}$, $10{\mu\ell}/{m\ell}$, $5{\mu\ell}/{m\ell}$, $2.5{\mu\ell}/{m\ell}$ of each solution and control group(not contained solution) 1. Solution extracted into methanol did not show clear zone against all bacteria samples. Only P.nigrescens, S. mutans and S. sanguis in solution extracted into ethylacetate, S. mutans and S. anguis in solutions extracted into chlorform and Butyl alcohol showed clear zone against all bacteria samples. Solution extracted into Butyl alcohol showed clear zone against 13 types of bacteria, excluding P. gingivalis. 2. In Solution extracted into methanol, the bacteria samples grew in the highest concentrated plate, showing minimal variation from control group. 3. In Solution extracted into Butyl alcohol, S. aureus, P. intermedia, E. corrodens, A. actinomycetemcomitans, B. forsythus, P. gingivalis et al. showed decreased growth in the highest concentrated plate. P. auruginosa, R. dentocariosa, A. viscosus, P. nigrescens, S. mutans et al. showed decreased growth at MIC $20{\mu\ell}/{m\ell}$ and S. sanguis showed decreased growth at MIC $10{\mu\ell}/{m\ell}$. 4. By analyzing the MIC level through considering the results from Kirby-Bauer method, Solution extracted into methanol did not reveal any antimicrobial effects and Solution extracted into Butyl alcohol showed the highest antimicrobial effects In conclusion, it can be used the extracts of Pulsatilla koreana as wide spectrum antimicrobial agent.

• 대한약리학회지
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• 제32권3호
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• pp.381-388
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• 1996

패혈증 환자에 흡입마취제를 투여하는 것은 혈역동학에 심각한 영향을 초래할 수 있다. 흡입마취제 투여시 내독소혈증흰쥐 대동맥 수축반응에 미치는 hydroxocobalamin의 영향을 알아보기위해, 내독소 (1.5 mg/kg)를 복강내 투여한 뒤 18시간 후에 대동맥을 적출하여 phenylephrine에 대한 수축반응과 이에 대한 흡입마취제 및 hydroxocobalamin의 작용을 알아보았다. 패혈증이 적절히 유발되었는 지를 확인하기 위해 RT-PCR을 이용하여 혈관 평활근에서 iNOS유도를 확인하였다. 내독소 처치에 의해 수축기 및 확장기 혈압이 대조군에 비해 유의하게 감소되었으나, 심박동수는 영향이 없었다. Phenylephrine에 대한 수축반응은 $10^{-8}\;M$부터 $10^{-5}\;M$까지 용량 의존적으로 증가했으며, 내독소혈증흰쥐 대동맥에서 수축반응이 억제되었다. halothane과 enflurane은 1 MAC 농도에서 phenylephrine에 의한 수축반응을 유의하게 억제했으나 isoflurane은 영향이 없었다. Hydroxocobalamin $(10^{-5}\;M)$은 흡입마취제의 종류 및 투여 유무에 관계없이 내독소혈증흰쥐 대동맥 수축반응을 증가시켰다. 이상의 결과로 미루어보아 hydroxocobalamin은 패혈증 환자의 흡입마취시 혈역동을 개선시킬 것으로 사료된다.

• 대한수의학회지
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• 제13권1호
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• pp.85-102
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• 1973

In the event a population of animals is exposed to ionizing radiation, proper disposition of animals will minimize loss to the farmer and protect the public from unwholesome meat and dairy products. Clinical response is an important factor in considering salvage of animals for food following exposure to ionizing radiation whether the dose is lethal or not. It is the purpose of this report to present the discussions of blood picture and growth of y ung growing rabbits exposed to cobalt-60 gamma-irradiation. The experimental animals were Hyrnalayan rabbits of 65 days old. The body weight for all animals of the time of irradiation was approximately 450g. The 96 rabbits used in the experiment were allotted t groups of eight. Rabbits in one group served as controls and the others were exposed to single doses of 100, 200, 300, 400, 500, 600 and 700 Roentgens (R). The exposure does rate averaged 15 R per minute. Central blood was obtained by heart puncture. Erythrocyte and leukocyte enumerations and, determinations of hemoglobin in blood and hematocrit value were made in the usual manner. Erythrocyte counts, concentrations of hemoglobin in blood, and hematocrit readings dropped to the lowest value between 1 and 4 weeks postirradiation, returning towards the control values. But recovery was not complete at the time of the last determination on 6 weeks postirradiation. There was a progressive decrease with increasing radiation does in the above values. Mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were not affected by the irradiation. After irradiation of each does the numbers of total leukocyte and the absolute numbers of various types of white cell began to decrease rapidly, and reached a minimum after 2 weeks, returning towards normal levels. But recovery was not complete at the time of the last determination on 6 weeks postirradiation. The reductions in total leukocyte and differential counts as the radiation does increases were apparent. Relative neutrophilia was observed in each irradiated group because of more expense of lymphocytes after irradiation. Growth rate of the rabbits was not affected by 100 R of whloe-body irradiation, while rabbits irradiated with 200, 300, 400 and 500 R showed marked depression of growth rate. As the radiation does increases, a depression of growth rate was apparent. The rabbits exposed to 600 and 700 R ceased from growing and recovery was not recognized during the experimental period.

• 지구물리와물리탐사
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• 제20권2호
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• pp.88-95
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• 2017

초계분지는 그릇과 같이 주변이 모암으로 둘러싸여 있는 닫힌 분지의 형태를 갖고 있다. 이러한 닫힌 분지에서는 입사한 지진에너지가 분지 내에서 다중 반사파를 형성하며 에너지의 집중이 발생하기 쉽다. 경상남도 합천군 초계면과 적중면에 위치한 타원형의 초계분지를 대상으로 상시미동 관측 조사를 수행하였다. 상시미동 관측 자료를 주파수 영역으로 변환하고 수평 대 수직성분 스펙트럼 비를 계산하였다. 이 결과로부터 각 관측 지점에 대한 기본 공명주파수를 측정하였다. 이전 연구에서 알려진 우리나라 퇴적층에 대한 고유주기와 두께의 경험적인 상관관계를 이용하여, 기본 공명 주파수로부터 초계분지의 퇴적층 두께 분포를 결정하였다. 급한 경사를 갖는 산지로 둘러싸인 분지 외곽에 비하여, 분지 내부의 관측점에서 기본 공명주파수가 최소 1.03 Hz로 낮으며, 이에 상응하는 퇴적층이 두껍고, 그 깊이는 최대 약 100 m에 이른다. 각 관측점에서 결정한 분지 깊이를 내삽하여 초계분지의 기반암 지형에 대한 3차원 분지 모델을 작성하였다.