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Antibacterial Activity of Herbal Complex ABHC for Development of Novel Therapeutic Agent Against Sepsis  

Lee, Ki Man (Department of Pharmacy, Sahmyook University)
Lee, Geum Seon (Department of Counseling Psychology, Sahmyook University)
Kim, Yu Ri (Department of Pharmacy, Sahmyook University)
Park, Jun Woo (Department of Pharmacy, Sahmyook University)
Boo, Kyung-Jun (Department of Pharmacy, Sahmyook University)
Yim, Dongsool (Department of Pharmacy, Sahmyook University)
Kang, Tae Jin (Department of Pharmacy, Sahmyook University)
Publication Information
Korean Journal of Pharmacognosy / v.50, no.3, 2019 , pp. 191-197 More about this Journal
Abstract
Sepsis, an infectious disease, is a life-threatening condition that arises when the response to infection causes injury to tissues and organs. The purpose of this study was to demonstrate whether ABHC-1 and ABHC-2, two functional extracts from herbal complex, have an anti-bacterial effect against Escherchia coli in vivo, in vitro experimental model. ABHC-1 and ABHC-2 showed the antibacterial activity against the bacteria by paper disc method. The minimum inhibitory concentration (MIC) was measured using alamar blue reagent. The MIC was shown at $60{\mu}g/ml$ from ABHC-1 and $500{\mu}g/ml$ from ABHC-2 against E. coli. We next examined the effect of ABHCs on the production of inflammatory cytokine, such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), which is related to the induction of inflammation, in RAW 264.7 cell. ABHC-1 and ABHC-2 increased $TNF-{\alpha}$ production of RAW 264.7 cell in a dose-dependent manner while two extract decreased $TNF-{\alpha}$ production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell in a dose-dependent manner. At a dose of $1{\times}10^8$ E. coli. i.p., non-treated mice were succumbed, while most of mice treated with ABHC-1 were survived. Therefore, our results suggest that ABHC-1 has anti-bacterial activity and can be a novel therapeutic agent against infectious diseases.
Keywords
Herbal complex; Sepsis; Antibacterial activity; Tumor necrosis $factor-{\alpha}$; Infection;
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