• Journal of Service Research and Studies
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• v.13 no.2
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• pp.24-40
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• 2023

As the aversion to advertising grows, native advertising has gained significant attention as a means to counteract this rejection. Native advertising is designed to seamlessly blend with other content on the page where they are displayed. They mimic the overall design and presentation of the platform, providing a natural exposure to consumers. Many companies utilize native advertising, but there is not much academic research on this topic. Therefore this study aims to examine the effectiveness of native advertising for social issues. The study looked at two types of native advertising: ads created by a company and ads sponsored by a company. Results suggested that consumers showed more positive attitudes towards a company's ads if they were sponsored by the company rather than created by the company. This study argued that this effect would be more pronounced for consumers who tended to buy ethically. To verify these hypotheses an online experiment was conducted revealing that on average consumers displayed the same attitude toward a company whether the native advertising was created or sponsored by a company. However, it was also discovered that consumers with a tendency for ethical consumerism formed more positive attitudes toward companies when the company sponsored native advertising as opposed to having created it. In practice, this study suggests how companies can improve the effectiveness of native advertising, such as implementing native advertising as a sponsor and being transparent about their advertising to appeal to consumers with high ethical consumption tendencies. This study expands the scope of research in areas related to native adverting and corporate social responsibilities.

• Animal Bioscience
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• v.36 no.6
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• pp.851-860
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• 2023

Objective: This study aims to evaluate the target genes of gga-miR-20a-5p and the regulated immune responses in the chicken macrophage cell line, HD11, by the exosome-mediated delivery of miR-20a-5p. Methods: Exosomes were purified from the chicken macrophage cell line HD11. Then, mimic gga-miR-20p or negative control miRNA were internalized into HD11 exosomes. HD11 cells were transfected with gga-miR-20a-5p or negative control miRNA containing exosomes. After 44 h of transfection, cells were incubated with or without 5 ㎍/mL poly(I:C) for 4 h. Then, expression of target genes and cytokines was evaluated by quantitative realtime polymerase chain reaction. Results: Using a luciferase reporter assay, we identified that gga-miR-20a-5p directly targeted interferon gamma receptor 2 (IFNGR2), mitogen-activated protein kinase 1 (MAPK1), mitogen-activated protein kinase kinase kinase 5 (MAP3K5), and mitogen-activated protein kinase kinase kinase 14 (MAP3K14). Moreover, the exosome-mediated delivery of gga-miR-20a-5p successfully repressed the expression of IFNGR2, MAPK1, MAP3K5, and MAP3K14 in HD11 cells. The expressions of interferon-stimulated genes (MX dynamin like GTPase 1 [MX1], eukaryotic translation initiation factor 2A [EIF2A], and oligoadenylate synthase-like [OASL]) and proinflammatory cytokines (interferon-gamma [IFNG], interleukin-1 beta [IL1B], and tumor necrosis factor-alpha [TNFA]) were also downregulated by exosomal miR-20a-5p. In addition, the proliferation of HD11 cells was increased by exosomal miR-20a-5p. Conclusion: The exosome-mediated delivery of gga-miR-20a-5p regulated immune responses by controlling the MAPK and apoptotic signaling pathways. Furthermore, we expected that exosomal miR-20a-5p could maintain immune homeostasis against highly pathogenic avian influenza virus H5N1 infection by regulating the expression of proinflammatory cytokines and cell death.

• Molecules and Cells
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• v.45 no.3
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• pp.122-133
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• 2022

The aim of this study was to investigating whether lncRNA H19 promotes myocardial fibrosis by suppressing the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β axis. Patients with atrial fibrillation (AF) and healthy volunteers were included in the study, and their biochemical parameters were collected. In addition, pcDNA3.1-H19, si-H19, and miR-29a/b-3p mimic/inhibitor were transfected into cardiac fibroblasts (CFs), and proliferation of CFs was detected by MTT assay. Expression of H19 and miR-29a/b-3p were detected using real-time quantitative polymerase chain reaction, and expression of α-smooth muscle actin (α-SMA), collagen I, collagen II, matrix metalloproteinase-2 (MMP-2), and elastin were measured by western blot analysis. The dual luciferase reporter gene assay was carried out to detect the sponging relationship between H19 and miR-29a/b-3p in CFs. Compared with healthy volunteers, the level of plasma H19 was significantly elevated in patients with AF, while miR-29a-3p and miR-29b-3p were markedly depressed (P < 0.05). Serum expression of lncRNA H19 was negatively correlated with the expression of miR-29a-3p and miR-29b-3p among patients with AF (r_s = -0.337, r_s = -0.236). Moreover, up-regulation of H19 expression and down-regulation of miR-29a/b-3p expression facilitated proliferation and synthesis of extracellular matrix (ECM)-related proteins. SB431542 and si-VEGFA are able to reverse the promotion of miR-29a/b-3p on proliferation of CFs and ECM-related protein synthesis. The findings of the present study suggest that H19 promoted CF proliferation and collagen synthesis by suppressing the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β axis, and provide support for a potential new direction for the treatment of AF.

• Journal of the Korean Orthopaedic Association
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• v.54 no.1
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• pp.72-77
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• 2019

Candida vertebral osteomyelitis (CVO) is a rare disease that is a complication of intravenous drug use, but recently it has been recognized as mostly an opportunistic infection. Because CVO appears to mimic pyogenic spondylodiscitis in terms of the clinical and radiologic presentations, it is often neglected in a usual clinical setting. The clinical, radiological, and biological characteristics of CVO are often used to make a differential diagnosis with vertebral osteomyelitis from other etiologies. Once an initial proper diagnosis was performed, the treatment relies on the prompt initiation of appropriate pharmacotherapy and serial monitoring of the clinical progress. This paper report late-onset CVO in two young patients who underwent a heart transplant surgery and had postoperative systemic candidiasis. These two cases are a good reminder of the potential of CVO in immunosuppressive patients treated with anti-fungal agents. This paper presents these two cases with a review of the relevant literature.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.36 no.3
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• pp.193-201
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• 2023

In this study, a nonlinear truss finite element is developed to analyze structures with negative Poisson effect-induced tensile buckling. In general, the well-known buckling phenomenon is a stability problem under a compressive load, whereas tensile buckling occurs because of local compression caused by tension. It is not as well-known as classical buckling because it is a recent study. The mechanism of tensile buckling can be briefly explained from an energy standpoint. The nonlinear truss finite element with a torsional spring is formulated because the finite element has not been reported in the literature yet. The post-buckling analysis is then performed using the generalized displacement control method, which reveals that the torsional spring plays an important role in tensile buckling. Structures that mimic a negative Poisson effect can be constructed using such post-buckling behaviors, and one of the possible applications is a mechanical switch. The results obtained are compared to those of analytical solutions and commercial finite element analysis to assess the validity of the proposed finite element model. The numerical results show that the developed finite element model could be a viable option for the basic design of nonlinear structures with a negative Poisson effect.

• Animal Bioscience
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• v.36 no.10
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• pp.1488-1498
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• 2023

Objective: α_S1-Casein is more closely associated with milk allergic reaction than other milk protein components. microRNA (miRNA) is a class of small non-coding RNAs that modulate multiple biological progresses by the target gene. However, the post-transcriptional regulation of α_S1-casein expression by miRNA in ruminants remains unclear. This study aims to explore the regulatory roles of miR-380-3p on α_S1-casein synthesis in goat mammary epithelial cells (GMEC). Methods: α_S1-Casein gene and miR-380-3p expression was measured in dairy goat mammary gland by quantitative real-time polymerase chain reaction (qRT-PCR). miR-380-3p overexpression and knockdown were performed by miR-380-3p mimic or inhibitor in GMEC. The effect of miR-380-3p on α_S1-casein synthesis was detected by qRT-PCR, western blot, luciferase and chromatin immunoprecipitation assays in GMEC. Results: Compared with middle-lactation period, α_S1-casein gene expression is increased, while miR-380-3p expression is decreased during peak-lactation of dairy goats. miR-380-3p reduces α_S1-casein abundance by targeting the 3'-untranslated region (3'UTR) of α_S1-casein mRNA in GMEC. miR-380-3p enhances β-casein expression and signal transducer and activator of transcription 5a (STAT5a) activity. Moreover, miR-380-3p promotes β-casein abundance through target gene α_S1-casein, and activates β-casein transcription by enhancing the binding of STAT5 to β-casein gene promoter region. Conclusion: miR-380-3p decreases α_S1-casein expression and increases β-casein expression by targeting α_S1-casein in GMEC, which supplies a novel strategy for reducing milk allergic potential and building up milk quality in ruminants.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.2C
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• pp.109-117
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• 2010

Gas hydrate dissociation can generate large amounts of gas and water in gas hydrate bearing sediments, which may eventually escape from a soil skeleton and form voids within the sediments. The loss of fine particles between coarse particles or collapse of cementation due to water flow during heavy or continuous rainfall may form large voids within soil structure. In this study, the effect of void formation resulting from gas hydrate dissociation or loss of some particles within soil structure on the strength of soil is examined. Glass beads with uniform gradation were used to simulate a gas hydrate bearing or washable soil structure. Glass beads were mixed with 2% cement ratio and 7% water content and then compacted into a cylindrical sample with five equal layers. Empty capsules for medicine are used to mimic large voids, which are bigger than soil particle, and embedded into the middle of five equal layers. The number, direction, and length of capsules embedded into each layer vary. After two days curing, a series of unconfined compression tests is performed on the capsule-embedded cemented glass beads. Unconfined compressive strength of cemented glass beads with capsules depends on the volume, direction and length of capsules. The volume and cross section formed by voids are most important factors in strength. An unconfined compressive strength of a specimen with large voids decreases up to 35% of a specimen without void. The results of this study can be used to predict the strength degradation of gas hydrate bearing sediments in the long term after dissociation and loss of fine particles within soil structure.

• Journal of Korea Water Resources Association
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• v.56 no.5
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• pp.337-345
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• 2023

Monitoring river microplastics is a challenging task since it is a time-consuming and high-cost process. The use of a physical model to have a better understanding of river microplastics' behaviors can complement the challenging monitoring process. However, there have been very limited studies on modeling river microplastics. In this study, therefore, we evaluated the applicability of one commonly used river water quality model, i.e., the Water Quality Analysis Simulation Program (WASP), in simulating the microplastic concentration in the river environment. We simulated the microplastic concentration in the Anyangcheon stream using the WASP's biochemical oxygen demand (BOD) and suspended solid (SS) variables as possible surrogate variables for the microplastics. Simulation analyses indicate that the SS state variable performs better than the BOD state variable to mimic the observed concentrations of microplastics. This is because of the characteristics of each water quality parameter; the BOD variable, a biochemical indicator, is inappropriate for modeling the behaviors of microplastics, which have generally constant biochemical features. In contrast, the SS variable, which has similar physical behaviors, followed the observed patterns of the microplastic concentrations well. To build a more advanced and accurate model for simulating the microplastic concentration, comprehensive and long-term monitoring studies of the river microplastics under different environmental conditions are needed, and the unit of microplastic concentration should be carefully addressed before its modeling application.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.1B
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• pp.95-109
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• 2008

We numerically analyzed the nonlinear shoaling, a plunging breaker and its accompanying energetic suspension of sediment at a bed, and a redistribution of suspended sediments by a down rush of preceding waves and the following plunger using SPH with a Gaussian kernel function, Lagrangian Dynamic Smagorinsky model (LDS), Van Rijn's pick up function. In that process, we came to the conclusion that the conventional model for the tractive force at a bottom like a quadratic law can not accurately describe the rapidly accelerating flow over a swash zone, and propose new methodology to accurately estimate the bottom tractive force. Using newly proposed wave model in this study, we can successfully duplicate severely deformed water surface profile, free falling water particles, a queuing splash after the landing of water particles on the free surface and a wave finger due to the structured vortex on a rear side of wave crest (Narayanaswamy and Dalrymple, 2002), a circulation of suspended sediments over a swash zone, net transfer of sediments clouds suspended over a swash zone toward the offshore, which so far have been regarded very difficult features to mimic in the computational fluid mechanics.

• Journal of the Korean Electrochemical Society
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• v.26 no.4
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• pp.43-55
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• 2023

Mass transport through nanoporous structures such as nanopores or nanochannels has fundamental electrochemical implications and many potential applications as well. These structures can be particularly useful for water treatment, energy conversion, biosensing, and controlled delivery of substances. Earlier research focused on creating nanopores with diameters ranging from tens to hundreds of nanometers that can selectively transport cationic or anionic charged species. However, recent studies have shown that nanopores with diameters of a few nanometers or even less can achieve more complex and versatile transport control. For example, nanopores that mimic biological channels can be functionalized with specific receptors to detect viruses, small molecules, and even ions, or can be made hydrophobic and responsive to external stimuli, such as light and electric field, to act as efficient valves. This review summarizes the latest developments in nanopore-based systems that can control mass transport based on the size of the nanopores (e.g., length, diameter, and shape) and the physical/chemical properties of their inner surfaces. It also provides some examples of practical applications of these systems.