• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1711-1717
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• 2011

The objective of this experiment was to evaluate the effect of dietary supplementation of glutathione (GSH) on health, solid feed consumption, nutrient intake, body weight gain (BWG), feed efficiency, blood metabolites and the occurrence of diarrhea in Holstein neonatal calves. The calves were fed plain milk as a control (CON) or milk with GSH supplementation. Sixteen calves were separated from their mothers immediately after birth, moved into individual cages and fed colostrum for the first three days. For GSH supplementation, three grams of GSH powder were mixed in 1.8 L of heat-treated milk and placed in a plastic bottle with a rubber nipple. The calves were fed GSH-supplemented milk only once out of four daily feedings. For the first 25 d, calves were fed 1.8 L of milk four times per day. Milk feeding frequency was reduced to three times per day from days 26 to 30, followed by twice a day from days 31 to 44, and once a day from days 45 to 49, after which they were weaned at day 50. Body weight gain (BWG), feed consumption, and growth performance were monitored until day 70. The dietary supplementation of GSH had no effect on daily feed intake and growth performance in growing calves. Hematological results revealed red blood cell distribution width (RDW) was lower, and mean corpuscular volume (MCV) was significantly higher in calves fed GSH. Serum lactate dehydrogenase (LDH) concentrations were lower in calves fed GSH. Rectal temperature at day 70 was higher in calves that did not receive GSH, while mean frequency of diarrhea and enteritis was less in calves fed GSH. It is concluded from the present study that BW gain, total dry matter intake (DMI), feed efficiency, and breathing rate did not differ between groups. However, there were some positive blood parameters and the mean frequency of diarrhea and enteritis was less in calves fed GSH compared to CON which did not receive GSH. With the results obtained, supplementation of GSH is highly recommended.

• Journal of Animal Science and Technology
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• 제58권1호
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• pp.2.1-2.10
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• 2016

Background: Heat stress adversely affects the physiological and metabolic status, and the productive performance of buffalo. Methods: The present study was conducted to explicate the effect of misting and wallowing cooling strategies during heat stress in lactating Murrah buffalo. The study was conducted for three months (May-July) of which first two months were hot dry and last month was hot humid. Eighteen lactating buffaloes, offered the same basal diet, were blocked by days in milk, milk yield and parity, and then randomly allocated to three treatments: negative control (no cooling), cooling by misting, and cooling by wallowing. Results: The results showed higher (P < 0.05) milk yield in buffaloes of misting and wallowing group compared to control during the experimental period however wallowing was found more (P < 0.05) effective during July (hot humid period). Both the treatments resulted into significant (P < 0.05) reduction in rectal temperature (RT) and respiratory rate (RR) compared to control animals during study period whereas wallowing was found to be effective on pulse rate (PR) only during July. Both treatments were resulted in mitigating the heat stress mediated decrease in packed cell volume (PCV), lymphocytopnoea and neutrophilia whereas decrease in total erythrocyte count (TEC) and monocytes was only mitigated by wallowing. Heat load induced alteration in serum creatinine and sodium concentration was significantly (P < 0.05) ameliorated by misting and wallowing whereas aspartate aminotransferase, alkaline phosphatase and superoxide dismutase activity, and reactive oxygen species concentration could be normalized neither by misting nor by wallowing. The significant (P < 0.05) increment in serum cortisol and prolactin levels observed in June and July period in control animals was significantly (P < 0.05) prevented by misting and wallowing. Conclusions: It can be concluded that misting and wallowing were equally effective in May and June (hot dry period) whereas wallowing was more effective during hot humid period in preventing a decline in milk production and maintaining physiological, metabolic, endocrine and redox homeostasis.

• 한국식품조리과학회지
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• 제22권2호
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• pp.204-212
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• 2006

$\beta$-glucan 첨가 우유식합의 이화학적 관능적 특성을 분석한 결과는 다음과 같다. 완성된 우유식빵의 무게는 $\beta$-glucan 첨가군이 대조군에 비해 차이가 없었다. 그러나 높이와 부피는 $\beta$-glucan 20% 첨가시까지 증가하다가 $\beta$-glucan 30% 첨가군에서 유의적으로 감소하였다. 수분함량은 $\beta$-glucan 첨가군이 높았으며, $\beta$-glucan 20 및 30% 첨가군은 저장 전 기간동안 유의적으로 높은 수치를 유지하였다. 기계적 조직감을 TPA로 측정한 결과, 식빵의 경도는 대조군과 $\beta$-glucan 첨가군 사이에 차이가 없었고 씹힘성과 응집성은 $\beta$-glucan 30% 첨가군이 유의적으로 높았다. 빵의 색상은 $\beta$-glucan 30% 첨가군을 제외하고 명도, 적색도 및 황색도에 있어 차이가 없었다. 전반적인 수용도는 대조군과 $\beta$-glucan 10 및 20% 첨가군이 6.8-6.9점, $\beta$-glucan 30% 첨가군은 3.4점을 받았다. 이상의 결과로부터 식빵 제조 시 $\beta$-glucan 첨가량은 20%까지는 긍정적인 효과를 나타내었다.

• 생명과학회지
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• 제14권4호
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• pp.676-682
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• 2004

버섯 추출물을 첨가하여 유산 발효시킨 버섯발효유와 이때 사용된 버섯 추출물 및 버섯발효유 상등액의 항당뇨 효과를 규명하고자 Sprague-Dawley 수컷에 streptozotocin을 50 mg/kg body weight씩 복강내 주사하여 당뇨를 유발시켜 검토하였다. 버섯발효유, 상등액 및 버섯 추출물을 식이 중에 10% (v/w)씩 동량 첨가 한 식이를 3주간 급여한 후 혈당치, 인슐린 농도 및 경구당부하실험을 실시하였다. 버섯발효유의 이화학적 성분을 분석 한 결과 단백질 2.87%, 지방 0.09%, 탄수화물 6.0%, 식이섬유 0.3%, 락토스 2.01%, 슈크로스 1.23% 및 칼슘과 철 성분을 각각 95.9 및 0.08 mg/l00 g함유하고 있었다. Streptozotocin-유발 대조군 당뇨쥐에 비해 버섯발효유 투여군에서 현저한 혈당강하 효과가 있었으며, 이러한 효과는 인슐린 농도증가에 의한 것으로 나타났다. 또한, 버섯발효유 상등액 및 버섯 추출물에도 혈당강하 효과가 있는 것으로 나타났다. 실험 종료시점에 실시한 경구당부하실험에서도 버섯발효유, 상등액 및 추출물 순으로 현저한 효과를 보였다. 이상의 결과로 볼 때 혈당강하 효과가 있는 버섯과 유산균을 접목한 버섯발효유 제조는 이들 상호간의 시너지 효과에 의해 당뇨성 흰쥐에서 현저한 항당뇨 효과를 발휘하였다.

• 한국포장학회지
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• 제12권1호
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• pp.63-73
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• 2006

한국의 시유 포장은 1937년 7월부터 현재 서울우유협동조합(그 당시 경성우유동업조합)이 1홉(hob : 180 ml, 0.048 standard gallon) 유리병에 지전(paper cap) 또는 콜크마개(cork cap)로 사용한 것이 최초였다. 그 이후(1945$\sim$1972)로 2홉 유리병 360 ml도 생산되었고 마개도 철전(iron cap)이나 plastic film을 씌운 뚜겅이 관모(egret, pappus)형태로 바뀌었다. 그 밖에 우유병이 부족하면 사이다병, 맥주병 등 유색유리용기가 사용되기도 하였다. 우유생산업체는 한국에서 서울우유협동조합만이 유일하게 있다가 1964년 3월 남양유업(주)이 창립되고, 1969년 현재 해태유업(그 당시 대한식품공사)이 설립, 1971년 5월 현재 매일유업(주)(그 당시 한국낙농유업) 등 여러 업체들이 점진적으로 설립되어 각 종의 낙농제품(dairy products, dairy manufactures)이 포장되어 생산되기 시작하였다. 그리고 1971년에 서울우유협동조합에서 처음으로 삼각형 폴리 에틸렌필름용기가 사용되었고 1972년에는 남양유업(주)의 종이용기인 Tetra pak 멸균팩을 수입하여 최초 사용하였다. 1974년경부터 사각형 폴리에틸렌필름포장과 플라스틱병 용기가 처음 선보였으며, 1977년에는 종이포장인 게이블톱 카튼팩(gable top carton pack)이 서주산업(주)에 의해 최초로 소개된 후 급격히 시장점유율이 확대되었고 용량에서도 180 ml에서 200 ml, 250 ml, 500 ml, 1,000 ml의 대형으로 서서히 바뀌었으며 그에 맞춰 소비량도 변화되었다. 그리고 파스퇴르 우유 490 ml의 포장을 1987년 9월에 국내 최초로 몸체는 고밀도 폴리에틸렌병(high density polyethylene bottle), 뚜껑은 저밀도 폴리에틸렌을 사용하였고 1992년 7월 남양유업은 다우(DOW)란 상품명으로 polyethylene병에 235g, 450g, 930g을 넣어 4각형 형태로는 최초 시판하였다.

• 대한영양사협회학술지
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• 제14권4호
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• pp.361-370
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• 2008

The purpose of this study is to provide basic data for the medical nutrition therapy of premature infants. The general characteristics, presence of metabolic disorders, hematological profile and feeding methods were compared between the premature infant group (<37 weeks, n=61) and the full-term infant group (37$\sim$42 weeks, n=165). Birth weight (p<0.0001), birth length (p<0.005), head circumference (p<0.0001), chest circumference (p<0.0001), and Apgar scores (p<0.0001) of the neonates were all statistically lower in the premature infant group. Jaundice cases (p<0.0001) were statistically higher in the premature infant group. White blood cell counts (WBC: p<0.005), mean corpuscular volume (MCV: p<0001), mean corpuscular hemoglobin (MCH: p<0.005), mean corpuscular hemoglobin concentration (MCHC: p<0.005), and mean platelet volume (MPV: p<0.05) were statistically lower in the premature infant group. The premature infant group were fed a higher rate of premature formula than breast milk and the full-term infant group were fed a high rate of human milk at a higher rate, showing differences in kinds of feeding methods (p<0.0001) between the two groups. An infant's birth weight showed a significantly positive correlation with the infant's birth length (p<0.0001), head circumference (p<0.0001), chest circumference (p<0.0001), and Apgar scores(p<0.0001). The birth length also showed a significantly positive correlation with both head circumference (p<0.05) and chest circumference (p<0.05). Head circumference showed a significantly positive correlation with chest circumference (p<0.0001) and Apgar scores (p<0.0001). Chest circumference showed a significantly positive correlation with Apgar scores (p<0.0001). In addition, the Apgar Score at of 1 minute after birth showed a significantly positive correlation with the Apgar score at of 5 minute after birth (p<0.0001).

• 한국식생활문화학회지
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• 제19권1호
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• pp.106-117
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• 2004

The factors of obesity related with food habits were assessed to provide information for nutrition education database. The subjects in this study were 234 primary school children in Busan. Children were classified into normal and obese groups(mildly obese, moderately obese and severely obese) by obesity index. In self-recognition of body image, only 28.6% of MI group considered themselves as 'overweight or obese'. 87.2% of the subjects controlled their weight method with exercise. With regard to meal speed, 64.5% of the subjects ate $fast({\leq}15\;min.)$. The study also found that 79.8% of the subjects ate breakfast, 56.4% of the subjects them were moderate by obese, 40.8% of the subjects ate snacks after dinner. Eating speed, meal volume and snacks money per day were significantly different in obesity. Preference for eating out was Korean Chinese, Western and Japanese food, in order. Favorite food for snack, such as ddukbokki, fruits, milk. fried foods and candy and caramel were significantly different in obesity(p<0.05). However hamberger, pizza and chocolate were not significantly different in obesity. The most preference snack for all the subjects were fruits and ice cream. In food preference, potato, dduk, meats, fishes, cheese, milk, ice cream were significantly different in obesity. The distasteful food for subjects were patbab, vegetables, shellfishes and salt-fermented foods. From above results, obesity of children was related to meal speed, meal volume and snacks motley per day. Therefore, these results suggest that continuous and practical nutrition education to change food habits art necessary to avoid child obesity.

• 대한소아치과학회지
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• 제36권3호
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• pp.358-366
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• 2009

유산균이 위장관과 구강건강에 probiotics로서 긍정적인 영향을 미치고 있음이 보고되고 있다. 이에 시판되는 유산균 발효유 5종과 우유를 선택하여 우식유발 원인균인 S. mutans에 대한 증식억제 및 biofilm 형성에 미치는 영향을 평가하고, 발효유의 산도, 완충능, 산생성능, 칼슘과 인의 함량을 측정하였다. 유산균 발효유에 S. mutans를 주입한 후 생균수를 측정한 결과 모든 발효유의 %가 증가함에 따라 S. mutans 수가 감소했으나 우유에서는 변화가 없었다. 10% 유산균 발효유에 S. mutans를 주입한 후 biofilm 형성 정도를 측정한 결과 액티브 GG, 불가리스는 biofilm 형성이 감소하였고, 이오는 유의한 변화가 없었으며, 투티, 에이스, 우유는 증가하였다(P<0.05). 유산균 발효유 및 우유의 산도를 측정한 결과 이오(3.48${\pm}$0.01), 투티(3.67${\pm}$0.02), 에이스(3.71${\pm}$0.02), 액티브 GG(4.04${\pm}$0.02), 불가리스(4.19${\pm}$0.02) 순으로 나타났다. 유산균 발효유의 산생성능을 평가한 결과 불가리스가 가장 높은 산생성능을 보였으며 액티브 GG, 에이스, 투티, 이오, 우유 순으로 산도 하강 경향을 나타냈다. 이상의 결과는 유산균 발효유가 초기산도 및 산생성능 측정 결과 우식유발 가능성이 있음을 나타냈다. 그러나 유산균 발효유 중에서 농후발효유가 우식유발균인 S. mutans를 억제하였고 biofilm 형성을 감소시켜 긍정적인 효과를 가지고 있음을 알 수 있었다.

• Bulletin of the Korean Chemical Society
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• 제34권9호
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• pp.2787-2794
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• 2013

Cholesterol and cholesterol oxidation products (COPs) may accumulate in foods of animal origin during processing or storage. An effective and sensitive analytical method was developed by increasing the UV absorption of compounds through derivatization by attaching a chromophore to the functional groups of cholesterols (cholesterol, 20-hydroxycholesterol, 7-ketocholesterol, cholestane-$3{\beta}$-$5{\alpha}$-$6{\beta}$-triol, 25-hydroxycholesterol, and $5,6{\alpha}$-epoxycholesterol). The influences of the reaction time, volume of reaction solvent, amounts of derivatizing reagent, and extraction solvents were investigated, as they may influence the reaction and extraction yield. The derivatized COPs were analyzed simultaneously on a C18 column (2.1 mm i.d. ${\times}$ 100 mm length, $3.5{\mu}m$ particle size) using a gradient elution with water and acetonitrile. The derivatized COPs showed increased sensitivity and selectivity in HPLC/UV-Vis. The LOD and LOQ were in the concentration ranges of 0.018-0.55 mg/kg and 0.059-1.84 mg/kg from the powdered milk. And the accuracy and precision were 78.1-116.7% and 1.1-9.9%, respectively.

• 한국식생활문화학회지
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• 제7권2호
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• pp.97-103
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• 1992

This research was conducted to survey the feeding methods of Korean women. The survey was executed for 355 mothers bringing up a child below 24 months in some part of Seoul in Korea from April to May, 1990. Incidence of breast feeding was 29.1% of the interviewed mothers in Seoul, which showed lower percentages than those of previous surveys. This research proved the major influencing factor generally depended on its mother rather than babies or labor condition. For example, the higher income and the higher educational degree of its mothers, they tended to prefer the bottle feeding to the others. Generally mothers knew the informations of infant nutrition very well, 84.5% of the interviewed mothers in Seoul had the opinion of 'breast feeding is better', but their knowledge actually didn't work. The source of mothers‘ informations on the infant nutrition showed the books and magazines, friend and relatives, grandmother were many by the order. But there were some discrepancies between the actual and needed source of information; i.e. mothers in Seoul mostly(44.8%) wanted to acquire their informations from mass communications. The reason of breast feeding showed 'for baby's health' was the greatest, and then 'immunity of breast milk' and 'baby's emotion' were great by the order, and the reason of bottle feeding showed 'not enough breast milk' was the greatest. There were no problems in feeding attitude and position of breast feeding, but there were problems in feeding volume, consistency of formula milk and feeding position of bottle feeding.