• 한국식생활문화학회지
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• 제11권2호
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• pp.217-228
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• 1996

Infanthood is a crucial period when mental and emotional developments take place along with physical growth. Adequate supply of nutrition is essential during this period and there is an urgent need for proper education and guidance on balanced nutrition. Therefore, this study was designed to assess to current status of lactation and to investigate mothers' perspectives on human milk and formular milk. As random samples for this study, a total of 352 mothers having infant under the age of 4 were surveyed. 27.3% of mother surveyed were breast-feeding, 38.0% were formula-feeding and 34.7% were mixed-feeding. According to the result of this study, the percentage of breast feeding was on the decrease, while the dependence on formular milk was on the rise. The main reason of formula-feeding was insufficient secretion of human milk on mothers part. The major factors that affect to select feeding method were employment status, degree of education, family income and age. Also, the interviewees desired the product fortified with special ingredients that are hot contained in formular milk currently available in the market. The most concerned problem of formula milk was indigestion and the interviewees wanted the formular milk to be improved digestibility.

• Journal of Nutrition and Health
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• 제34권1호
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• pp.23-29
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• 2001

This study was conducted to investigate the concentrations and infants intake of sodium and potassium from human milk during the first 5 months of lactation. The sodium concentrations of the milk during the lactation appeared 21.1, 18.1, 15.9, 12.4, 10.6 and 11.4 mg/100g at 0.5, 1, 2, 3, 4, and 5 months of lactation respectively. The potassium concentrations of the milk 48.5, 43.3, 40.8, 39.9, 40.5 and 38.5 respectively, Na/K ratio of the milk were 0.76, 0.75, 0.70, 0.56, 0.46 and 0.53(mEqu\\mEq). Sodium intakes of breast-fed infants were 106.9, 108.4, 90.6, 82.5 and 95.6mg/day during the lactation. Potassium intakes of the infants were 255.7, 276.0, 289.7, 294.6, 320.3 and 318.3mg/day. Sodium and potassium concentrations in infant formula averaged 23.62 and 67.61 mg/100g which was 1.90 and 1.69 times as those of breast milk at 3 month of lactation. Commercial whole milks had 39.53 and 135.22mg/100g of sodium and potassium respectively which was 3.19 and 3.39 times as those of the milk. From this study, reevaluation of sodium and potassium intakes if breast-fel infants was merited and the regulation of that minerals in infant formula is need to lower the renal solute load of formula-fed infants.(Korean J Nutrition 34(1):23-29, 2001)

• Journal of Dairy Science and Biotechnology
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• 제35권2호
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• pp.99-104
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• 2017

The global consumption of fructose in the form of high-fructose corn syrup (HFCS) has increased considerably over the past several decades. The current intake of HFCS exceeds that of other major caloric sweeteners such as sucrose. Fructose has potentially adverse effects on human metabolism. Therefore, the objective of this study was to analyze the monosaccharide contents of 13 sweetened milk-based beverages by using high-performance liquid chromatography (HPLC). The total monosaccharide (fructose and glucose) contents of various sweetened milk-based beverages ranged from 0.60 g/100 mL (cheese) to 26.03 g/100 mL (maple caramel snack), while milk showed only 0 g/100 mL monosaccharides. The fructose-to-glucose ratio of various popular sweetened milk-based beverages ranged from not-detected (ND) to 2.24 g/100 mL, but the content of glucose was higher than that of fructose in only three samples, namely, strawberry flavored milk, shrimp chips, and maple caramel snack. The analyzed results revealed important basic information about monosaccharides in various milk-based sweetened beverages, presenting ideas for future fructose research.

• Journal of Nutrition and Health
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• 제29권2호
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• pp.213-222
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• 1996

The most abundant long-chain polyunsaturated fatty acid in brain lipids is docosahexaenoic acid(C22 : 6 N-3, DHA). It is incorporated into nerve tissues mostly in utero and during the first year of life. DHA in brain is derived from either pre-formed DHA in human milk or by infant hepatic synthesis from linolenic acid in milk. This study was designed to investigate the effects of DHA supplementation on fatty acid profiles in maternal plasma lipid and breast milk. Twenty lactating women participated in the study. Seven women took 3g of fish oil per day and vitamin E for 28 days starting from the day of giving birth. Five women consumed 1.5g of fish oil as well as tivamin E, and the rest took vitamin E supplements for the same period of time. Dietary questionnaires and 3 consecutive 24-h recalls were collected to evaluate theri nutritional status and food habits. Finding that DHA intake from fish was not significantly different among three experimental groups, the partcipants were instructed to continue eating their usual home diets. Milk samples were taken on the day of giving birth, as well as the 7th, 14th and 28th day being the supplement phase, and finally 2 weeks after the cessating of DHA supplements. The amounts of the fish oil supplements produced significant dose-dependent increased in the DHA content of milk and plasma, but to a lesser degree. Base-line for 28 days raised the level to 2.05$\pm$0.43% and 1.5g/day supplement produced DHA levels of 1.02$\pm$0.19%. The results of this study indicated that relatively small amount of dietary DHA supplementation significantly elevats DHA content in milk. This would clearly elevate the infant's DHA intake which in turn may have implications for the infant's brain development.

• 한국식품영양과학회지
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• 제38권5호
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• pp.644-648
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• 2009

Bacillus subtilis로 발효한 두유의 암세포 증식 저해 효과를 알아보기 위하여 AGS 인체 위암 세포를 이용하여 실험을 수행하였다. 두유와 발효두유 모두 암세포 성장 저해 효과가 나타났으며, 발효두유에서 그 효과가 더욱 증진되었다. 이러한 결과는 apoptosis에 의해 암세포 증식이 저해된 것으로 사료되며 DAPI staining을 통해 뚜렷한 apoptotic body를 관찰할 수 있었다. 발효두유를 처리한 암세포에서 apoptosis를 유발하는 인자인 bcl-2의 발현은 감소하고, 억제하는 인자인 bax의 발현은 발효하지 않은 두유보다 상대적으로 증가하였다. 또한 종양억제 유전자인 p53과 p21의 발현 역시 발효하지 않은 두유보다 발효두유에서 더 증가되었다. 따라서 발효두유의 항암 효과는 이들 유전자의 발현을 조절하여 유도된 apoptosis에 의한 것으로 보이며, 발효두유와 발효하지 않은 두유의 항암효과의 차이는 발효 균주와 발효과정중 생성된 여러 가지 발효산물들이 암세포 성장 억제에 영향을 끼친 것으로 생각된다.

• 여성건강간호학회지
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• 제20권1호
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• pp.14-28
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• 2014

Purpose: This study was to identify relationships of maternal psychosocial factors including mother's mood state, childcare stress, social support and sleep satisfaction with breastfeeding adaptation and immune substances in breast milk, especially secretory immunoglobulin A (sIgA) and transforming growth factor-beta 2 (TGF-${\beta}2$). Methods: Data were collected from 84 mothers who delivered full-term infants by natural childbirth. Structured questionnaires and breast milk were collected at 2~4 days and 6 weeks postpartum. Data were analyzed using descriptive statistics, Pearson's correlation, multiple linear regression, and generalized estimating equation (GEE). Results: Scores for the breastfeeding adaptation scale were significantly related with child care stress, mood state and social support. Mother's anger was positively correlated with the level of sIgA in colostrum (p<.01). Immune substances of breastmilk was significantly influenced by time for milk collection (p<.001) and the type of breastfeeding (sIgA, p<.001, TGF-${\beta}2$, p=.003). Regression analysis showed that breastfeeding adaptation could be explained 59.1% by the type of breastfeeding, childcare stress, the Profile of Mood States, emotional support and sleep quality (F=16.67, p<.001). Conclusion: The findings from this study provide important concepts of breastfeeding adaptation program and explanation of psychosocial factors by immune substances in breast milk. Future research, specially, bio-maker research on breast milk should focus on the ways to improve breastfeeding adaptation.

• 분석과학
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• 제35권6호
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• pp.229-236
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• 2022

모유의 지질대사체에 대한 연구는 축산유래 유제품의 경우와 비교하여 매우 제한적으로 이루어져 있다. 본 연구는 지용성 대사체 연구의 선행연구로서, 모유중 극성 및 비극성 지질 중의 주요 지방산 조성에 대한 세부적 정량법 및 구조동정을 목적으로 수행되었다. 인지질을 포함한 극성 지질과 비극성 지질의 분리를 위하여 silica gel을 활용한 정제법을 개발하였으며, 효과적인 methyl ester 및 pyridylcarbinol ester 유도체화 방법을 적용하여 개별 분획의 세부적 지방산조성을 분석한 결과, 16 종의 필수지방산을 확인하였으며, 이와 함께 29 종의 미량 지방산이 확인되어, 구조 분석을 실시하였다. 측쇄 지방산의 경우, 15-18 개의 탄소를 보유한 iso 및 anteiso 지방산이 주요한 것으로 확인되었다.

• Journal of Nutrition and Health
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• 제21권2호
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• pp.129-133
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• 1988

수유기간의 경과에 따른 모유중의 비중, 총고형분, 단백질 함량 변화를 살펴 보고자 수유부 27명을 대상으로 분만후 15일부터 150일까지 추적하여 채취한 총112개 모유시료로 부터 얻은 결과는 다음과 같다. 즉 모유 비중의 경시적인 변화는 15일경에 1.0298(S.D. 0.0044, n=25), 30일경에 1.0304(S.D. 0.0046, n=24), 60일경에 1.0270(S.D. 0.0035, n=20) 90일경에 1.0293(S.D. 0.0055, n=18), 120일경에 1.0254(S.D. 0.0038, n=16), 150일경에 1.0268(S.D. 0.0039, n=9)로서 나타났고 수유기간이 경과함에 따라 유의하게 감소하는 경향을 보였다. 총고형분은 모유 100$m\ell$중에 15일 경에 12.48g(S.D. 1.23, n=25), 30일경에 12.31g(S.D. 1.31, n=24) 60일경에 12.47g (S.D. 1.45, n=20), 90일경에 11.81g (S.D. 1.85, n=18), 120일경에 11.44g(S.D. 0.92, n=16) 150일경에 11.88 (S.D. 0.89, n=9)으로 나타났고 수유기간의 경과에 따른 유의한 차이는 보이지 않았다. 모유 100$m\ell$중 단백질함량은 1.07~1.46g의 범위였고, 수유기간별 변화는 15일경에 1.46g (S.D. 0.20, n=25), 30일 경에 1.32g (S.D. 0.19, n=24) 60일경에 1.15g (S.D. 0.10, n=20), 90일경에 1.09g (S.D. 0.17, n=18), 120일경에 1.11g (S.D. 0.17, n=16), 150일 경에 1.07g (S.D. 0.18, n=9)으로 수유기간이 경과 함에 따라 유의하게 감소하는 경향을 보였다. 또한 수유기간 15, 30, 50, 90, 120, 150일에 수집한 모유시료 전체의 총평균 단백질함량은 1.20g (S.D. 0.14, n=27, 총시료수 112)로 나타났다.

• Toxicological Research
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• 제29권1호
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• pp.53-60
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• 2013

Studies on milk transfer of drugs in non-human primates (NHPs) are among the crucial components in the assessment of peri- and postnatal toxicity because of the similarity between NHPs and humans. To evaluate the milk transfer of valproic acid (VPA) in NHPs, the toxicokinetics of VPA, an antiepileptic drug, were studied in pregnant cynomolgus monkeys. VPA was administered once daily to pregnant cynomolgus monkeys at doses of 0, 30, 90, and 270 mg/kg by oral gavage from Day 100 of gestation (GD 100) to Day 31 of lactation (LD 31). Concentrations of VPA and its metabolite, 4-ene-VPA, in the maternal plasma on GD 100, GD 140, and LD 30, and concentrations of VPA and 4-ene-VPA in the offspring plasma and milk on LDs 30 and 31, respectively, were quantified using liquid chromatography tandem mass spectrometry (LC/MS/MS). After administration of a single oral dose of VPA to pregnant monkeys on GD 100, the concentrations of VPA and 4-ene-VPA were generally quantifiable in the plasma of all treatment groups up to 24 hr after administration, which showed that VPA was absorbed and that the monkeys were systemically exposed to VPA and 4-ene-VPA. After administration of multiple doses of VPA to the monkeys, VPA was detected in the pup's plasma and in milk taken on LD 30 and LD 31, respectively, which showed that VPA was transferred via milk, and the pup was exposed to VPA. Further, the concentration of VPA in the milk increased with an increase in the dose. Extremely low concentrations of 4-ene VPA were detected in the milk and in the pup plasma. In conclusion, pregnant monkeys were exposed to VPA and 4-ene-VPA after oral administration of VPA at doses of 30, 90, and 270 mg/kg/day from GD 100 to LD 31. VPA was transferred via milk, and the VPA exposure to the pup increased with an increase in the dose of VPA. The metabolite, 4-ene VPA, was present in extremely low concentrations (< 0.5 ${\mu}g/ml$) in the milk and in the pup plasma. In this study, we established methods to confirm milk transfer in NHPs, such as mating and diagnosis of pregnancy by examining gestational sac with ultrasonography, collection of milk and pup plasma and determination of toxicokinetics, using cynomolgus monkeys.

• 한국식품영양학회지
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• 제5권2호
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• pp.84-89
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• 1992

Concentrations of major minerals(Ca, p, Na, K, Mg) and trace elements (Fe, Zn) were measured in human milk samples collected from 19 selected, healthy lactating women at 2~5 days, and at 4, 8 and 12 weeks postpartum. Decreases in the contents of m) or minerals and trace elements were found with the increase of time postpartum. Mean Ca contents of 2~5 days, 4 week, 8 week and 12 week in human milk were 243.02$\pm$50.90ug/ml, 295.0$\pm$65.70ug/ml, 295.0$\pm$41.50ug/ml, 349.90$\pm$83.50ug/ml, respectively And mean P contents of 2~5 days, 4 week, 8 week and 12 week in human milk were 134.70$\pm$49.4ug/ml, 134.90$\pm$19.70ug/ml, 117.60$\pm$18.90ug/ml, 130.60$\pm$28.20ug/ml, respectively. Ca/P of them were 1.81, 2.19, 2.20, 2.68, respectively. Mean Mg contents of them were 22.30$\pm$5.60ug/ml, 25.70$\pm$3.70ug/ml, 25.10$\pm$3.60ug/ml, 32.10$\pm$4.50ug/ml, respectively. And mean Na contents of them were 171.20$\pm$90.50ug/ml, 158.70$\pm$47.00ug/ml, 104.50$\pm$24.70ug/ml, 115.90$\pm$12.50ug/ml, respectively. Mean K contents of them were 506.10$\pm$156.10ug/ml, 520.90$\pm$55.00ug/ml, 370.10$\pm$29.00ug/ml, 468.40$\pm$75.70ug/ml, respectively. Na/K of them were 0.34, 0.30, 0.28, 0.25, respectively. Mean Fe contents of them were 2.80$\pm$1.80ug/ml, 2.70$\pm$0.40ug/ml, 2.60$\pm$0.40ug/ml, 2.10$\pm$0.10ug/ml, respectively. And mean Zn contents of them were 3.70$\pm$1.60ug/ml, 2.78$\pm$9.70ug/ml, 2.70$\pm$0.70ug/ml, 2.30$\pm$0.20ug/ml, respectively, These determinations will provide the basic information on the variability of major minerals and trace elements as lactation proceeds and on the composition of components between term and preterm milk.