• Biomolecules & Therapeutics
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• 제18권1호
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• pp.92-98
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• 2010

Breast cancer has been estimated as one of the most common causes of cancer death among women. The major cause of death from breast cancer is the metastatic spread of the disease from the primary tumor to distant sites in the body. Lycopene is one of the major carotenoids in fruits and vegetables including tomatoes. Epidemiological studies have shown that the dietary intake of lycopene is associated with decreased risk of cancer. Although mounting evidence shows the chemopreventive effect of lycopene, the role of lycopene in the prevention of metastatic potential of breast cancer has not been determined yet. In the present study, we investigated the inhibitory effect of lycopene on invasive and migratory phenotypes of two highly aggressive breast cancer cell lines, H-Ras-transformed MCF10A human breast epithelial cells (H-Ras MCF10A) and MDA-MB-231 human breast cancer cells. Here, we report that lycopene significantly inhibits invasion and migration as well as proliferation of H-Ras MCF10A and MDA-MB-231 cells. This study suggested an in vitro anti-cancer and anti-metastatic potential of lycopene. We also showed that activations of ERKs and Akt were inhibited by lycopene in H-Ras MCF10A cells, suggesting that the ERKs and Akt signaling pathways may be involved in lycopene-induced anti-proliferative and/or anti-invasive/migratory effects in these cells. Taken in conjunction with the fact that breast cancer metastasis is one of the most lethal malignancies in women, our findings may provide useful information for the application of lycopene in establishing strategy to prevent the metastatic breast cancer.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.325.3-326
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• 2002

An imbalance between matrix metalloproteinase (MMP)-2 and its endogenous inhibitor. tissue inhibitor of metalloproteinase (TIMP)-2 causes the degradation of the extracellular matrix associated with pathological events including invasion. metastasis and angiogenesis. Since TIMPs are secreted molecules. they have the potential to be used for gene therapy of certain tumors. (omitted)

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 Molecular and Cellular Response to Toxic Substances
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• pp.140-140
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• 2002

Glial cell-derived neurotrophic factor (GDNF) is a potent neurotrophic factor that enhances survival of midbrain doparminergic neuron and is a member of the transforming growth factor-b superfamily. GDNF and its receptors are widely distributed in brain and are believed to be involved in the control of neuron survival, proliferation and differentiation.(omitted)

• Journal of Pharmaceutical Investigation
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• 제39권1호
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• pp.51-54
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• 2009

${\beta}ig$-h3, is a TGF-${\beta}$-induced gene product, extracellular matrix protein with 68 kDa MW(683 amino acids) and has been known for its possible roles in cell adhesion, spreading, migration and proliferation. To minimize a proteolytic degradation of ${\beta}ig$-h3, ${\beta}ig$-h3 incorporated chitosan sponge was prepared and its effects on fibroblast adhesion and migration were investigated. And its wound healing efficacy was evaluated in deep 2nd degree burn rabbit ear wound model. ${\beta}ig$-h3 enhanced fibroblast adhesion and proliferation. In histological observation, a significant over-proliferation of epidermal regeneration was observed in ${\beta}ig$-h3/chitosan dressing applied wound while epidermal regeneration was not proceeded yet in chitosan only treated wound. ${\beta}ig$-h3/sponge dressing could enhance epidermal regeneration.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2001년도 추계학술대회 및 정기총회
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• pp.105-105
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• 2001

In an attempt to provide useful information on the development of an artifitial nerve tubing, proliferative and migrative properties of two glioma cell lines, C6 rat glioma cells and Hs683 human glioma cells, were examined. The present study shows that C6 cells proliferated more rapidly than Hs683 cells. The Hs683 cells are more adequate for the development of nerve tubing since unlike C6 cells, they are of human origin and known to be non-tumorigenic. In order to enhance proliferative and migrative abilities of Hs683 cells for the application as an artificial nerve tubing, we studied the effect of glial cell-derived neurotrophic factor (GDNF) on Hs683 cells. Cells were seeded in the scaffolds (polymer constructs), fabricated with type I collegen and alginate modified with cinnamoyl moiety, in the presence or absence of GDNF Stimulatory effect of GDNF on the proliferation and migration of Hs683 cells cultured in the scaffolds is currently under investigation. In addition, possible neuroprotective activities of natural products which inhibit staurosporine-induced apoptosis of glioma cells are also being studied.

• Nutrition Research and Practice
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• 제9권3호
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• pp.304-312
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• 2015

BACKGROUND/OBJECTIVES: International students face dissimilar food environments, which could lead to changes in dietary behaviors and anthropometric characteristics between before and after migration. We sought to examine the risk factors, including dietary behaviors, acculturation, and demographic characteristics, related to overweight subjects residing in South Korea. SUBJECTS/METHODS: We conducted a cross-sectional study, collecting data from 142 Chinese international students (63 males, 79 females) in 2013. RESULTS: The mean age of the subjects was 25.4 years, and almost half of them immigrated to South Korea to earn a master's degree or doctoral degree (n = 70, 49.3%). Chinese international students showed an increase in skipping meals and eating speed, but a decrease in the frequency of fruit and vegetable consumption in South Korea compared to when they lived in China. We found a statistically significant increase in weight (69.4 ${\rightarrow}$ 73.9 kg) and BMI (22.4 ${\rightarrow}$ $23.8kg/m^2$) for male subjects (P < 0.001) but no change for female subjects. We also found that overweight subjects were more likely to be highly acculturated and male compared with normal-weight subjects. CONCLUSION: Among Chinese international students living in South Korea, male and more highly acculturated subjects are more vulnerable to weight gain. This study provides useful information to design tailored nutrition intervention programs for Chinese international students.

• 한국미생물·생명공학회지
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• 제49권4호
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• pp.594-602
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• 2021

The NLRP3 (nucleotide-binding domain, leucine-rich repeat family pyrin domain containing 3) inflammasome plays an important role in the initiation of inflammatory responses, through the recognition of pathogen-associated molecular patterns and tumor progression, including tumor growth and metastasis. In this study, we examined the effects of defective NLRP3 on the growth, migration, and invasiveness of hepatocellular carcinoma (HCC) SK-Hep1 cell. First, HCC SK-Hep1 cells were transfected with human NLRP3 targeting LentiCRISPRv2 vector using the CRISPR-Cas9 system, and NLRP3 deficiency was confirmed by RT-qPCR and western blotting. NLRP3 deficient SK-Hep1 cells showed delayed cell growth and decreased protein expression of PI3K, p-AKT, and pNF-κB when compared to NLRP3 complete SK-Hep1 cells. In addition, NLRP3 deficiency arrested the cell cycle at G1 phase through an increase in p21 and a reduction in CDK6. NLRP3 deficient SK-Hep1 cells also showed significantly delayed cell migration, invasion, and wound healing. The expression of epithelial-mesenchymal transition signaling molecules, such as N-cadherin and MMP-9, was found to be dramatically decreased in NLRP3 deficient SK-Hep1 cells compared to NLRP3 complete SK-Hep1 cells.

• 대한지리학회지
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• 제51권4호
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• pp.525-539
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• 2016

본 연구는 사후분포를 예측하는 베이지안 추정기법의 일환인 마르코프 체인 모형을 적용하여 직업요인 인구이동에 따른 직종별 취업자의 공간적 분포에 나타나는 변화를 예측하였다. 이를 위해 인구이동의 사유 중 직업요인 이동량을 추출하여 직업을 요인으로 하는 인구이동 패턴을 파악하고, 직업요인 인구이동의 추이확률 산출 값을 토대로 채프만-콜모고로프 방정식을 구축하여 장래 지역별 취업자 분포와 직종분포의 변동성을 예측하였다. 분석결과, 서울의 취업자 분포가 감소할 것으로 예측되나 직종 중 단순노무 종사자는 증가할 것으로 예측되었다. 전문가 및 관련 직의 경우 수도권과 일부 광역시를 제외한 모든 지역에서 증가할 것으로 추정되었고, 강원, 충청지역은 전체 직업군의 취업자 분포에 있어 증가세를 나타낼 것으로 예측되었다. 본 연구 결과는 향후 지역 노동시장의 원활한 인력수급이 가능하도록 유입, 유출될 가능성이 높은 인력 및 직종을 중심으로 직업훈련, 취업알선 등 고용지원 서비스를 통해 사전 대비하는 방안 마련에 기초자료로 활용될 수 있다.

• Clinical and Experimental Reproductive Medicine
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• 제32권3호
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• pp.287-293
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• 2005

Objective: To evaluate the usefulness of serum concentrations of macrophage migration inhibitory factor (MIF) of patients with ovarian cysts for differential diagnosis of endometrioama. Method: From Jan. 2003 to Dec. 2004, preoperative serum MIF levels were assessed in 28 women with endometrioma, 32 with benign epithelial tumor, 23 with functional and simple cysts, 22 with benign mature cystic teratoma, and 25 women without ovarian tumor as control. MIF levels were determined using an ELISA (Quantikine Human MIF immunoassay, R&D Systems, Inc., USA). Results: Mean MIF levels were higher in all groups with benign tumors than control (all p<0.01), but there was no significant difference between benign tumor groups (p=0.95). There was no significant correlation between MIF levels and tumor volume, body mass index (BMI) (p=0.635, 0.674 respectively) Serum MIF level had significant correlation with count of WBC and neutrophils (p=0.008, 0.024 respectively), but had no correlation with count of lymhocytes and monocytes (p=0.688, 0.294 respectively). Conclusions: This study showed a marked increase in MIF concentrations in the peripheral blood of patients with endometrioma, but there was no significant difference with other benign tumors. Serum MIF level had significant correlation with count of WBC and neutrophils. These suggest serum MIF level has no usefulness for differential diagnosis of endometrioma from other benign ovarian cysts.

• 경제분석
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• 제26권1호
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• pp.34-83
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• 2020

본 연구는 통일한국에서 남한 중심으로 추진되는 통일정책을 분석하고 북한 주민들이 소득재분배와 관련한 의사결정에 참여할 때 어떤 정책이 채택되는지 살펴본다. 구체적으로 두 가지 정책을 분석한다. 첫째는 남한 중심의 이주 및 재분배정책으로서 남한의 중위 투표자가 가장 선호하는 이주 비율과 세율이 채택된다. 둘째는 북한이 참여하는 재분배정책으로서 이주가 자유롭고 통일한국에서 중위투표자가 세율을 선택하며 동 세율이 남북한 모든 주민들에게 동일하게 적용된다. 북한 주민들의 남한 이주문제를 명시적으로 분석하기 위해서 이주 의사결정을 내생화한다. 남한으로 이주를 선택하지 않는 북한 주민들은 새로운 정부를 구성하거나 통일 직후 남한으로부터의 재정 독립을 선언할 수 있다. 본 연구에서는 남한 중심의 재분배정책 하에서 소득재분배가 이루어지지 않는다는 것을 보인다. 북한의 중위투표자는 소득재분배가 이루어지지 않는 상황에서 재정독립을 통해 자체적으로 소득재분배를 수행하는 것을 선호한다. 북한 참여 정책 하에서는 보다 적극적인 재분배 정책이 이루어지고 재정독립이 일어나지 않는다는 것을 보인다.