• Korean Journal of Clinical Laboratory Science
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• v.46 no.4
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• pp.136-139
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• 2014

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the severe nosocomial infectious agents. The traditional diagnostic methods including biochemical test, antibiotic susceptibility test and PCR amplification are time consuming and require much work. The Surface enhanced Raman spectroscopy (SERS) biosensor is a rapid and powerful tool for analyzing the chemical composition within a single living cell. To identify the biochemical and genetic characterization of clinical MRSA, all isolates from patients were performed with VITEK2 gram positive (GP) bacterial identification and Antibiotic Susceptibility Testing (AST). Virulence genes of MRSA also were identified by DNA based PCR using specific primers. All isolates, which were placed on a gold coated nanochip, were analyzed by a confocal Raman microscopy system. All isolates were identified as S. aureus by biochemical tests. MRSA, which exhibited antibiotic resistance, demonstrated to be positive gene expression of both femA and mecA. Furthermore, Raman shift of S. aureus and MRSA (n=20) was perfectly distinguished by a confocal Raman microscopy system. This novel technique explained that a SERS based confocal Raman microscopy system can selectively isolate MRSA from non-MRSA. The study recommends the SERS technique as a rapid and sensitive method to detect antibiotic resistant S. aureus in a single cell level.

• Applied Microscopy
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• v.37 no.3
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• pp.199-208
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• 2007

We have made an optical device to study the wave optics phenomena, such as image and diffraction pattern, constructive and destructive interference, by direct operation of laser beam and optical lenses. It consists of laser beam, goniometer, objective lens, intermediate lens, projection lens, CCD system, and computing system. As a result of the performance test, we were able to magnify samples up to 44 times with the resolution of about $5{\mu}m$. It is expected to help EM users understanding more easily principles of transmission electron microscopy (TEM).

• Proceedings of the SAREK Conference
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• 2006.06a
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• pp.95-98
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• 2006

Filtration by fibrous filter is one of the Principle methods used for removing pollutant particles in the liquid. Because of the increasing need to protect both human health and valuable devices from exposure to fine particles, filtration has become more important. Filters have been developed with modified surface charge characteristics to capture and adsorb particles by electrokinetic interaction between the filter surface and particles contained in water. The main purposes of this study are to develop and evaluate the performance evaluation of the apparatus for making a positively charged porous filter media and to analyze the surface characteristics of the filter media for capturing negavitely charged contaminants mainly bacteria and virus from water. The experimental apparatus consists of a mixing tank, a vacuum pumping system, a injection nozzle, a roller press and a controller. The filter media is composed of glass fiber(50-750 nm), cellulose($10-20{\mu}m$) and colloidal charge modifier. The characteristics of filter media is analyzed by SEM(Scanning Electron Microscopy), AFM(Atomic Force Microscopy) and quantified by measuring the zeta potential values.

• Information Display
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• v.4 no.2
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• pp.3-6
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• 2003

This paper describes a MacMic System developed for Flat Panel Display. The MacMic System usually is used for testing of Mother Glass of TFT and Color Filter. They are normally consisted of microscopy system, illumination system and panel stage system.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1446-1449
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• 2008

Immune system is composed of multiple cells with distinct functions, and immune responses are orchestrated by complex and dynamic cell-cell interactions. Therefore, each cell behavior and function should be understood under right spatio-temporal context. Studying such complexity and dynamics has been challenging with conventional biological tools. Recent development of new technologies such as state of art imaging instruments and microfabrication techniques compatible with biological systems have provided many exciting opportunities to dissect complex and dynamic immune cell interactions; new microscopy techniques enable us to observe stunning dynamics of immune system in real time. Microfabrication permits us to manipulate microenvironments governing molecular/cellular dynamics of immune cells to study detailed mechanisms of phenomena observed by microscopy. Also, microfabrication can be used to engineer microenvironments optimal for specific imaging techniques. In this presentation, I am going to present an example of how these two techniques can be combined to tackle challenging problems in immunology. Obviously, this strategy can readily be applied to many different fields of biology other than immunology.

• Applied Microscopy
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• v.28 no.3
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• pp.391-398
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• 1998

The morphological phenotype on ocellus of Drosophila rdgC mutant was observed with electron microscope. The result showed the particular phenotype that was not found in other retinal degenarative mutants. The most distinct difference was the orientation of photoreceptor cells. The photoreceptor cells did not attached to corneagenous cells but dropped under corneagenous cells and assembled around newly formed space. Enormous multivesicle bodies caused by the degeneration of photoreceptor cells were frequently found. Rhabdomeres were also severely degenerated in consequence of the mutant. Another degeneration was found in a part of photoreceptor cell, but the degeneration of subrhabdomeric cisternae (SRC) was not found. It was a ovious difference of rdgC comparing with other two retinal degenerative mutants, rdgA and rdgB. As a result, rdgC mutant was affected on the attachment between photoreceptor cells and corneageneous cells, and it suggested the defect of cell-cell attachment. In addition, rdgC mutant was accompanied by the defect not only in retina but nerve system. The results were agreed to the reference discussion that the rdgC molecule is exist in the nerve.

• International Journal of Precision Engineering and Manufacturing
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• v.4 no.1
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• pp.23-29
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• 2003

Stencil is used normally as a mask for solder pasting on pad of a printed circuit board (PCB). The objective of this study is to develop a stencil cutting system and determine the optimal conditions to make good-quality stencil by using a Nd:YAG laser. The effects of process parameters such as laser power, type of mask, gas pressure, cutting speed and pulse duration on the cut edge quality were investigated. In order to analyze the cut surface characteristics (roughness, kerfwidth, dross) optical microscopy, SEM microscopy and roughness measurements were used. As a result, the optimal conditions of cutting process parameters were determined, and the practical feasibility of the proposed system was also examined by using a commercial Gerber file for PCB stencil manufacturing.

• The Korean Journal of Ceramics
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• v.7 no.2
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• pp.80-84
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• 2001

The anisotropic CdS platelets were synthesized in the lamellar bilayer phase region of the octylamine-water binary system. The influence of the synthesis conditions of the system components on morphology and size of the platelets was examined. Atomic force microscopy (AFM) and high-resolution transmission electron microscopy (HRTEM) studies have shown thickness and face size of the synthesized particles. Platelets with face sizes ranging from 50 to 250 nm and thickness from 10 to 30 nm have been synthesized at room temperature. In addition, HRTEM micrographs show that the synthesized platelets are poly crystal.

• Structural Engineering and Mechanics
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• v.82 no.6
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• pp.747-756
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• 2022

This study presents the nonlinear free and forced vibrations of a cracked atomic force microscopy (AFM) cantilever by using the modified couple stress. The cracked section of the AFM cantilever is considered and modeled as rotational spring. In the frame work of Euler-Bernoulli beam theory, Von-Karman type of geometric nonlinear equation and the modified couple stress theory, the nonlinear equation of motion for the cracked AFM is derived by Hamilton's principle and then discretized by using the Galerkin's method. The semi-inverse method is utilized for analysis nonlinear free oscillation of the system. Then the method of multiple scale is employed to investigate primary resonance of the system. Some numerical examples are presented to illustrate the effects of some parameters such as depth of the crack, length scale parameter, Tip-Mass, the magnitude and the location of the external excitation force on the nonlinear free and forced vibration behavior of the system.

• 한국전자현미경학회:학술대회논문집
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• 1999.05a
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• pp.84-84
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• 1999