• Childhood Kidney Diseases
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• 제15권1호
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• pp.29-37
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• 2011

신장질환은 같은 병명의 환자 일지라도 개개인의 증상과 치료에 대한 반응 정도가 다를 수 있기 때문에 신장병을 전공하는 임상의사는 신장병리에 대한 폭 넓은 지식이 치료에 많은 도움을 줄 수 있다. 이 논문에서는 생검 현장에서의 조직을 나누는 방법부터 기본적인 조직처리 과정까지를 설명함으로써 병리의 이해를 넓히고자 하였다. 광학현미경 판독을 위해서는 기본적인 특수 염색의 색깔 감별, 정상 사구체와 비정상 사구체의 감별점 등을 설명하였다. 면역형광 현미경 소견의 이해를 돕기 위하여 전형적인 막성사구체신병증과 IgA 신증의 침착 형태를 대표적으로 설명하였다. 전자현미경 판독을 위해서 면역 복합체인 고전자 밀도의 침착이 잘 생기는 부위를 질환별로 설명하여 참고가 되도록 하였고 전자현미경으로만 감별 가능한 질환들을 설명하였다. 이 논문은 신장질환의 병리학적 이해를 돕기 위한 소개에 불과하므로, 개별 질환의 판독을 위해서는 다른 신장병리 교과서나 문헌을 참고하여야 한다.

• Journal of Korean Neurosurgical Society
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• 제58권6호
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• pp.513-517
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• 2015

Objective : Microscopic indocyanine green (ICG) angiography is useful for identifying the completeness of aneurysm clipping and the preservation of parent arteries and small perforators. Neuroendoscopy is helpful for visualizing structures beyond the straight line of the microscopic view. We evaluated our prototype of endoscopic ICG fluorescence angiography in swine, which we developed in order to combine the merits of microscopic ICG angiography and endoscopy. Methods : Our endoscopic ICG system consists of a camera, a light source, a display and software. This system can simultaneously display real-time visible and near infrared fluorescence imaging on the same monitor. A commercially available endoscope was used, which was 4 mm in diameter and had an angle of $30^{\circ}$. A male crossbred swine was used. Results : Under general anesthesia, a small craniotomy was performed and the brain surface of the swine was exposed. ICG was injected via the ear vein with a bolus dose of 0.3 mg/kg. Visible and ICG fluorescence images of cortical vessels were simultaneously observed on the display monitor at high resolution. The real-time merging of the visible and fluorescent images corresponded well. Conclusion : Simultaneous visible color and ICG fluorescent imaging of the cortical vessels in the swine brain was satisfactory. Technical improvement and clinical implication are expected.

• Journal of Periodontal and Implant Science
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• 제37권2호
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• pp.251-263
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• 2007

The present study was performed to evaluate the effects of Tetracycline-HCI on the microstructure change of SLA implant surface according to application time. In the Tetracycline-HCI group, 6 implants were rubbed with sponges soaked $50mg/m{\ell}$ Tetracycline-HCI solution for O.5min., lmin., 1. 5min., 2min., 2.5min. and 3min. In the saline group. another 6 implants conditioned with sponges soaked saline using same methods. One implant wasn't conditioned anything. Then, the changes of surface roughness values were evaluated by optical interferometer & specimens were processed for scanning electron microscopic observation. The results of this study were as follows: 1. In both Tetracycline-HCI group & saline group, there are no significant differences between surface roughness values before & after surface detoxification. And in scanning electron microscopic observation. there are slightly changes of implant surface structures but this changes were not significant by comparison with no treatment implant surface. 2. In the changes of surface roughness values & the scanning electron microscopic observation, there were no significant differences between saline & Tetracycline-HCI groups. In conclusion, the detoxification with $50mg/m{\ell}$ Tetracycline-HCI within 3 minutes can be applied for treatment of peri-implantitis in SLA surface implants. without surface microstructure changes.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2003년도 봄 학술발표회 논문집
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• pp.85-92
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• 2003

Generally, ordinary portland cement(OPC) is widely used for grouting to reduce permeability of ground under the foundations of structures. But, it is hard to be injected into the microscopic voids, fissures and crevices in soil or rock formation for the OPC material. Therefore new method what is called MSG(Micro Silica Grouting) has been developed recently to improve the weak point of the OPC material. In this case study, in order to verify performance of the MSG's water cut-off, trial injections were performed in rear of CIP(Cast in Place Pile) on the site A(weathered soil) and B(alluvial soil) that are constructed for the subway No. 9 nowadays. To take the proper grouting method of the MSG in the trial injecting, the injections are carried out for grouting types(constant pressure or fixed Quantity) and grouting methods(1.5shot or 2.0shot) and to confirm the effects of water cut-off and the injection range of the MSG, the tests of permeability and indicator(phenolphthalein) response were performed before and after the injection. Through the tests results, we could affirm the effects of water cut-off of the MSG and the injection range for the weathered and alluvial soil layers near the Han River. Finally we could make sure the application of the MSG method in actual construction under the layers.

• 한국임상수의학회지
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• 제9권2호
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• pp.457-466
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• 1992

An 11 years old Irish Setter bitch was euthanlzed and necropsied because of clinical findings such as severe purulent nasal discharge and formation of large tumor mass, 8 ${\times}$8cm in size, in the abdominal cavity. A complete unilateral mastectomy had been carried out twice 14 and 22 months before necropsy. The surgically removed masess of the mammary glands had been diagnosed as malignant mixed tumor in each time. Grossly, tumor masses were observed in nasal cavity, infralumbar lymph node, lung, abdominal cavityn and brain. Microscopic findings of the surgically removed masses consisted of tumor epithelial cells, tumor hyaline cartilage-like structures and abundant connective tissues. The mass of the lymph node had similar microscopic features to those of the original malignant mixed tumor of the mammary glands. The tumor osseous tissue and osteoid were observed in the abdominal cavity, lung, and brain. Myoepithelial cells were frequently found on association with the metastatic tumors. From the results, it was concluded that malignant mixed tumor of the mammary glands metastasized to the infralunbar lymph node, abdominal cavity, lung and brain. In addition, the observation in this study supported two theories at the same time that the bone in malignant mixed tumor arises by endochondral ossification of the cartilage formed by the myoepithelial cells and arises by intramembranous ossification of stromal connective tissue or transformed myeopithelial cells. Solid carcinoma of the nasal epielia and granulosa cell tumor were also diagnosed in a mass of the nasal cavity and of the ovaries respectively.

• 대한의용생체공학회:의공학회지
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• 제17권4호
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• pp.479-490
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• 1996

To utilize collagen as an implantable biomateriall the mcct widely used bovine skin origin Type I collagen was investigated Pepsin treated, Type I atelocollagen was extracted and crosslinked by the ultraviolet(W) ray with wavelength of 254nm or by various concentrations of glutaraldehyde to produce collagen membranes. The crosslink rates of the specimens were observed by a polarized light microscope, a scanning electron microscope, and a Fourier transform infrared (FT-lR) spectrometer. The followings are concluded 1. The collagen membranes produced by both 2.5% glutaraldehyde solution and 254nm UV ray irra- diation demonstrated similar morphologies on polarized light microscopic and scanning electron microscopic views. 2. The chemical structures of the crosslinked membranes by glutaraldehyde over 2.5% in concentrations revealed similar intensities to that of the UV ray irradiated one in FT-lR investigation. 3. To obtain optimal croulink in bovine stalin origin Type I atelocollagen, 2.5% glutaraldehyde solution or UV ray irradiation with 254nm wavelength is acceptable.

• Journal of Chest Surgery
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• 제22권3호
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• pp.373-383
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• 1989

Glutaraldehyde is known as an ideal preservatives for pericardial heterograft, and many laboratories used this chemicals for preparing tissue valves, pericardial patches and MVOP [monocusp ventricular outflow patch] so we tried to find out the appropriate concentration and ingredients of the Glutaraldehyde for the preparing bovine pericardium. We selected 50 calves, aged about 2 years, and procured their pericardia. These were divided 6 groups such as fresh group, treated with only antibiotics, treated with Glutaraldehyde 0,5%, 0.625 %, 0.75 %, and 0.875 %, and our experiments included microbial culture test, tensile strength measurement and microscopic examination. On microbial culture, there were no growth on 1 week and 4 weeks after preparation with all kind of Glutaraldehyde, but on 4 weeks after only antibiotics treatment [Penicillin, Streptomycin, Kanamycin, Amphotericin -B] E.coli and candida albicans were observed. On tensile strength test, 0.625 % and 0.75 % Glutaraldehyde were revealed as the best preservatives for bovine pericardium and compared to other commercial products they kept more desirable tensile strength. On light and electron microscopic examinations, Glutaraldehyde treated pericardia had much regular and compact collagen fibers and preserved more normal structures, but there were no difference between the different concentration of the Glutaraldehyde. We concluded that 0.625% and 0.75 % Glutaraldehyde were the best concentration for preservation of bovine pericardium in our experiment.

• Bulletin of the Korean Chemical Society
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• 제16권9호
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• pp.873-885
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• 1995

The local structural and thermodynamical properties of blends A-B/H of a diblock copolymer A-B and a homopolymer H are studied using the polymer reference interaction site model (RISM) integral equation theory with the mean-spherical approximation closure. The random phase approximation (RPA)-like static scattering function is derived and the interaction parameter is obtained to investigate the phase transition behaviors in A-B/H blends effectively. The dependences of the microscopic interaction parameter and the macrophase-microphase separation on temperature, molecular weight, block composition and segment size ratio of the diblock copolymer, density, and concentration of the added homopolymer, are investigated numerically within the framework of Gaussian chain statistics. The numerical calculations of site-site interchain pair correlation functions are performed to see the local structures for the model blends. The calculated phase diagrams for A-B/H blends from the polymer RISM theory are compared with results by the RPA model and transmission electron microscopy (TEM). Our extended formal version shows the different feature from RPA in the microscopic phase separation behavior, but shows the consistency with TEM qualitatively. Scaling relationships of scattering peak, interaction parameter, and temperature at the microphase separation are obtained for the molecular weight of diblock copolymer. They are compared with the recent data by small-angle neutron scattering measurements.

• The Plant Pathology Journal
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• 제35권5호
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• pp.417-424
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• 2019

Melanose, caused by Diaporthe citri, is one of severe diseases in citrus, a major economic resource in Jeju island. To reduce the usage amount of organic synthetic fungicide, bio-sulfur was tested as an alternative chemical to control citrus melanose in the present study. Direct antifungal activity of bio-sulfur against D. citri was determined through in vitro experiment using artificial nutrient media. Disease severity of melanose on bio-sulfur pretreated citrus leaves was lower than that on untreated ones. To illustrate the mechanism of disease suppression by bio-sulfur, infection structures were observed with a fluorescent microscope and a scanning electron microscope. In fluorescent microscopic observation, most conidia rarely germinated. In addition, hyphal growth on leaves pretreated with bio-sulfur was inhibited compared to that on untreated ones. In scanning electron microscope images of bio-sulfur pretreated leaves, surfaces of most conidia were shrunk while hyphae were morphologically changed and frequently branched. Such microscopic observations were also found for leaves pretreated with a commercial fungicide Dithianon. These results suggest that bio-sulfur may be used to control citrus melanose as an environment friendly alternative to organic synthetic fungicides

• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2002년도 춘계학술발표대회 논문집
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• pp.181-183
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• 2002

The accurate permeability for preform is critical to model and design the impregnation of fluid resin in the composite manufacturing process. In this study, the in-plane and transverse permeability for a woven fabric are predicted numerically through the coupled flow model which combines microscopic with macroscopic flow. The microscopic and macroscopic flow which are flows within the micro-unit and macro-unit cell, respectively, are calculated by using 3-D CVFEM(control volume finite element method). To avoid checker-board pressure field and improve the efficiency on numerical computation, A new interpolation function for velocity is proposed on the basis of analytic solutions. The permeability of plain woven fabric is measured through unidirectional flow experiment and compared with the permeability calculated numerically. Based on the good agreement of the results, the relationships between the permeability and the structures of preform such as the fiber volume fraction and stacking effect can be understood. The reverse and the simple stacking are taken in account. Unlike past literatures, this study is based on more realistic unit cell and the improved prediction of permeability can be achieved. It is observed that in-plane flow is more dominant than transverse flow in the real flow through preform and the stacking effect of multi-layered preform is negligible. Consequently, the proposed coupled flow model can be applied to modeling of real composite materials processing.