• Title/Summary/Keyword: Microscopic image analysis

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Analysis of Wear Debris for Machine Condition Diagnosis of the Lubricated Moving Surface (기계윤활 운동면의 작동상태 진단을 위한 마멸분 해석)

  • Seo, Yeong-Baek;Park, Heung-Sik;Jeon, Tae-Ok
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.5
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    • pp.835-841
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    • 1997
  • Microscopic examination of the morphology of wear debris is an accepted method for machine condition and fault diagnosis. However wear particle analysis has not been widely accepted in industry because it is dependent on expert interpretation of particle morphology and subjective assessment criteria. This paper was undertaken to analyze the morphology of wear debris for machine condition diagnosis of the lubricated moving surfaces by image processing and analysis. The lubricating wear test was performed under different sliding conditions using a wear test device made in our laboratory and wear testing specimen of the pin-on-disk-type was rubbed in paraffine series base oil. In order to describe characteristics of debris of various shape and size, four shape parameters (50% volumetric diameter, aspect, roundness and reflectivity) have been developed and outlined in the paper. A system using such techniques promises to obviate the need for subjective, human interpretation of particle morphology in machine condition monitoring, thus to overcome many of the difficulties in current methods and to facilitate wider use of wear particle analysis in machine condition monitoring.

Stress analysis of the CR lens using the chrome conversion (Chrome 변환을 이용한 CR 렌즈의 미세응력 시각화)

  • Kim, Yong-Geun
    • Journal of Korean Ophthalmic Optics Society
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    • v.10 no.1
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    • pp.9-15
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    • 2005
  • The polariscope to measure the microscopic stress in CR lens consists of light source polarizer, model, polarizer, CCD, computer, chrome conversion orderly and the principal-stressed difference, (${\sigma}_1-{\sigma}_2$) and the fringe order n were measured by analyzing two components of light wave $E_1$ and $E_2$ following each polarizer's steps. The two-dimensional model could be determined from the fact that the optical axes of sample concide with the principal-stress directions. The bi-refringence acted to a light wave and the phase retardation were in proportion to the principal-stressed difference(${\sigma}_1-{\sigma}_2$) and the intensity of final light wave was proportioned to $sin2({\Delta}/2)$ and when ${\Delta}/2=n{\pi}$ (n=0, 1, 2, ${\ldots}$) the extinction occurs. Photoelastic's image by microscopic stress could analyzed using chrome conversion, and the image showed clearly.

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Microscopic Analysis of High Lithium-Ion Conducting Glass-Ceramic Sulfides

  • Park, Mansoo;Jung, Wo Dum;Choi, Sungjun;Son, Kihyun;Jung, Hun-Gi;Kim, Byung-Kook;Lee, Hae-Weon;Lee, Jong-Ho;Kim, Hyoungchul
    • Journal of the Korean Ceramic Society
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    • v.53 no.5
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    • pp.568-573
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    • 2016
  • We explore the crystalline structure and phase transition of lithium thiophosphate ($Li_7P_3S_{11}$) solid electrolyte using electron microscopy and X-ray diffraction. The glass-like $Li_7P_3S_{11}$ powder is prepared by the high-energy mechanical milling process. According to the energy dispersive X-ray spectroscopy (EDS) and selected area diffraction (SAD) analysis, the glass powder shows chemical homogeneity without noticeable contrast variation at any specific spot in the specimen and amorphous SAD ring patterns. Upon heating up to $260^{\circ}C$ the glass $Li_7P_3S_{11}$ powder becomes crystallized, clearly representing crystal plane diffraction contrast in the high-resolution transmission electron microscopy image. We further confirm that each diffraction spot precisely corresponds to the diffraction from a particular $Li_7P_3S_{11}$ crystallographic structure, which is also in good agreement with the previous X-ray diffraction results. We expect that the microscopic analysis with EDS and SAD patterns would permit a new approach to study in the atomic scale of other lithium ion conducting sulfides.

Evaluation of Air Void System and Permeability of Latex-Modified Concrete by Image Analysis Method

  • Jeong, Won-Kyong;Yun, Kyong-Ku;Hong, Seung-Ho
    • International Journal of Concrete Structures and Materials
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    • v.19 no.1E
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    • pp.41-48
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    • 2007
  • Addition of latex to concrete is known to increase its durability and permeability. The purpose of this study is to analyze air void systems in latex-modified concretes using a reasonable and objective method of image analysis with such experimental variables as water-cement (w/c) ratios, latex contents (0%, 15%) and cement types (ordinary portland cement (OPC), high-early strength (HES) cement and very-early strength (VES) cement). The results are analyzed by spacing factor, air volume (content) after hardening, air void distribution and structure. Additionally, air void systems and permeability of latex-modified concrete (LMC) are compared by a correlation analysis. The results are as follows. The LMC of the same w/c ratio showed better air entraining (AE) effect than OPC with AE water reducer. The VES-LMC showed that the quantity of entrained air below $100{\mu}m$ increased more than four times. For the case of HES-LMC, microscopic entrained air between the range of 50 to $500{\mu}m$ increased greater than 7 times even in the absence of anti-foamer. Although spacing factor was measured rather low, the permeability of latex-modified concrete was good. It is construed that air void system does not have a considerable effect on the property of latex-modified concrete, but latex film (membrane) has a definite influence on the durability of LMC.

Three Dimensional Confocal Imaging and Biomedical Image Analysis (3차원 Confocal Imaging과 생체 영상 분석)

  • Lee, Yim-Kul
    • Proceedings of the KOSOMBE Conference
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    • v.1995 no.11
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    • pp.257-261
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    • 1995
  • Confocal laser scanning microscopy (CLSM)는 기존의 coherent or incoherent microscopic imaging 보다 횡축 방향 (lateral direction)으로 고해상도를 가지며, 층과 층 사이를 구분하는 광축 방향 (axial direction)의 optical sectioning에 의해 샘플의 3D 구조를 고해상도로 영상화함으로써 3D 구조 및 생체 기능 분석을 가능하게 해 준다. 본 논문에서는 CLSM에 의한 3D 영상화 원리와 촛점면 부근에서 얻어지는 광세기 분포, 얻어진 2D slice 영상의 시각화 및 응용에 대해 논의된다.

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Analysis of Motional Characteristics of Sperm Using Image Processing (영상처리를 이용한 정자의 운동 특성 분석)

  • Shim, Hoon-Sup;Yi, Won-Jin;Park, Kwang-Suk;Paick, Jae-Seung
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.31B no.11
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    • pp.109-115
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    • 1994
  • In this paper, we developed an analyzing method of the motional characteristics of sperm, using image processing technology. Without the aid of a dedicated image-processor, this processing of a personal computer(PC) and a simple image processing board. The image processing board is used for acquiring images from a microscopic imaging source. The PC processes the images from the board and computes the parameters of motional characteristics of sperms. The algorithm of the site detection of sperms and the 'Match Matrix Method' is noteworthy. After comparing the results of our method with those of the manual method, and with those of the method using a dedicated image-processor, we concluded that our method is useful and reliable.

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A scheme of extracting age-related wrinkle feature and skin age based on dermoscopic images (피부 현미경 영상을 통한 피부 특징 추출 및 피부 나이 도출 기법)

  • Choi, Young-Hwan;Hwang, Een-Jun
    • Journal of IKEEE
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    • v.14 no.4
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    • pp.332-338
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    • 2010
  • Usually, mage feature extraction methods are performed as a pre-processing step in many applications including image retrieval, object recognition, and image indexing. Especially, in the image texture analysis, texture feature extraction methods attempt to increase texture contrast to make it easier to extract the texture features from the image. One of the distinct textures in microscopic skin image is the wrinkle, and its features could provide various useful information for the age-related applications. In this paper, we propose a scheme to extract age-related features from the skin images and improve its accuracy in the skin age estimation.

Computerized Image Analysis of Micronucleated Reticulocytes in Mouse Bone Marrow (컴퓨터 이미지 분석법을 이용한 마우스 골수세포에서 소핵의 계수)

  • 권정;홍미영;고우석;정문구;이미가엘
    • Toxicological Research
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    • v.18 no.4
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    • pp.369-374
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    • 2002
  • The present study was performed to validate an automated image analysis system (Loats Automated Micronucleus Scoring System) for the mouse bone marrow micronucleus assay, comparing with conventional microscopic scoring. Two studies were conducted to provide slides for a comparison of micro-nucleated polychromatic erythrocytes (MNPCEs) values collected manually to those collected by the auto-mated system. Test article A was used as an example of a compound negative for the induction of micronuclei and test article B was wed as a micronucleus-inducing agent to elicit a positive response. Cyclophosphamide was included to provide an positive control in two studies. Bone marrow samples were collected 24 h after administration of test article A and B in male ICR mice. The cells were fixed with absolute methanol and stained with May-Grunwald and Giemsa. The number of MNPCEs was determined by the analysis of 1000 total PCEs per bone marrow sample. In addition to micronucleus scoring, an index of bone marrow toxicity based on PCE ratio (% of PCEs to total erythrocytes) was determined for each sample. The automated and manual scoring was similar when the MNPCEs incidence induced by each test article was less than 10. However manual scoring was able to effectively enumerate micronucleated PCEs in mouse bone marrow when MNPCEs incidence was more than 10, such as cyclophosphamide treatment. Conversely, PCE ratio was superior in computer-assisted image analysis. Taken together, it is suggested that improvement of the automated image analysis may be necessary to render the automatic scoring as sensitive as manual scoring for routine counting of micronuclei, especially because it is superior in objectivity and high throughput scoring.

The Effect of Low-intensity Pulsed Ultrasound on Osteogenesis in Mini-pig Mandibles (Mini-pig 하악골에서 저강도진동초음파가 골형성에 미치는 영향)

  • Yun, Yeong-Eun;Lee, Jun;Min, Seung-Ki;Kim, Sang-Jung
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.33 no.6
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    • pp.467-477
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    • 2011
  • Purpose: The objective of this study was to examine the affected period and the amount of bone formation during osteogenesis of intramembranous bone using low-intensity pulsed ultrasound (LPUS) $in$ $vivo$. Methods: Xeno-bone (Bio-oss) and autogenous bone were grafted bilaterally into mini-pig mandibles. The left mandible served as the control and the other mandible was treated with 3 MHz, 160 mW (output, 0.8 mW) ultrasound stimulation for 7 days 15 minutes per day. The mini-pigs were sacrificed at 1, 2, 4, and 8 weeks, and micro computed tomography (${\mu}CT$), a microscopic examination, and a statistical analysis were performed on the specimens. Results: Based on a computerized image analysis of the ${\mu}CT$ scans, the experimental group had an average 150% more new bone formation than that in the control group. The effect of LPUS continued during the post operative 2 weeks. The histomorphological microscopic examination showed similar results. Conclusion: Our results suggest the LPUS had an effect on early intramembranous bone formation in vivo.

Movement of graphene grain boundary and its interaction with defects during graphene growth (그래핀 결정입계의 이동 및 결함과의 상호작용)

  • Hwang, Suk-Seung;Choi, Byung-Sang
    • The Journal of the Korea institute of electronic communication sciences
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    • v.9 no.3
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    • pp.273-278
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    • 2014
  • On poly and single crystalline Cu substrates, the graphene was synthesized by chemical vapor deposition(CVD). Optical microscopic images which were not possible to show the detailed characterization of graphene growth were adjusted and analyzed using image analyzing software. As a result it was possible to show the detailed growth mechanism of graphene by utilizing the image analysis. Nucleation of graphene on Cu grain boundary and its growth behavior into Cu grain are shown. In addition, the movement of graphene grain boundary interacting with Cu grain boundary and pinholes during growth was illustrated in detail, and the cause and result are discussed as a result of those interactions.