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The Effect of Low-intensity Pulsed Ultrasound on Osteogenesis in Mini-pig Mandibles  

Yun, Yeong-Eun (Department of Oral and Maxillofacial Surgery, Sun Dental Hospital)
Lee, Jun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Wonkwang University)
Min, Seung-Ki (Department of Oral and Maxillofacial Surgery, School of Dentistry, Wonkwang University)
Kim, Sang-Jung (Department of Oral and Maxillofacial Surgery, Sun Dental Hospital)
Publication Information
Maxillofacial Plastic and Reconstructive Surgery / v.33, no.6, 2011 , pp. 467-477 More about this Journal
Abstract
Purpose: The objective of this study was to examine the affected period and the amount of bone formation during osteogenesis of intramembranous bone using low-intensity pulsed ultrasound (LPUS) $in$ $vivo$. Methods: Xeno-bone (Bio-oss) and autogenous bone were grafted bilaterally into mini-pig mandibles. The left mandible served as the control and the other mandible was treated with 3 MHz, 160 mW (output, 0.8 mW) ultrasound stimulation for 7 days 15 minutes per day. The mini-pigs were sacrificed at 1, 2, 4, and 8 weeks, and micro computed tomography (${\mu}CT$), a microscopic examination, and a statistical analysis were performed on the specimens. Results: Based on a computerized image analysis of the ${\mu}CT$ scans, the experimental group had an average 150% more new bone formation than that in the control group. The effect of LPUS continued during the post operative 2 weeks. The histomorphological microscopic examination showed similar results. Conclusion: Our results suggest the LPUS had an effect on early intramembranous bone formation in vivo.
Keywords
Low-intensity pulsed ultrasound; in vivo; Intramembranous bone; ${\mu}CT$;
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