• 한국축산식품학회지
• /
• 제38권2호
• /
• pp.362-375
• /
• 2018

This study proposed a rapid microscopic examination method for pork freshness evaluation by using the self-assembled hyperspectral microscopic imaging (HMI) system with the help of feature extraction algorithm and pattern recognition methods. Pork samples were stored for different days ranging from 0 to 5 days and the freshness of samples was divided into three levels which were determined by total volatile basic nitrogen (TVB-N) content. Meanwhile, hyperspectral microscopic images of samples were acquired by HMI system and processed by the following steps for the further analysis. Firstly, characteristic hyperspectral microscopic images were extracted by using principal component analysis (PCA) and then texture features were selected based on the gray level co-occurrence matrix (GLCM). Next, features data were reduced dimensionality by fisher discriminant analysis (FDA) for further building classification model. Finally, compared with linear discriminant analysis (LDA) model and support vector machine (SVM) model, good back propagation artificial neural network (BP-ANN) model obtained the best freshness classification with a 100 % accuracy rating based on the extracted data. The results confirm that the fabricated HMI system combined with multivariate algorithms has ability to evaluate the fresh degree of pork accurately in the microscopic level, which plays an important role in animal food quality control.

• Journal of Korean Neurosurgical Society
• /
• 제40권6호
• /
• pp.406-411
• /
• 2006

Objective : The authors have developed a procedure, termed posterior microscopic lesionectomy, that creates a minimal laminotomy site according to the location of the shifted disc using the $METRx^{TM}$ system in the lumbar spine. This study compared the usefulness and surgical outcomes of this procedure with those of traditional standard lumbar discectomy. Methods : From June 2003 to June 2004, Twenty-two patients with one-level radiculopathy due to lumbar disc herniation underwent posterior microscopic lesionectomy with the assistance of an operating microscope and the $METRx^{TM}$ tubular retractor. Surgical results of the new procedure were compared to those of 39 patients who underwent traditional lumbar discectomy from April 2003 to September 2004. All patients were evaluated for pain score, clinical assessment according to the VAS, and Roland-Morris scores pre-operatively and at 1, 3, 6, and 12 months post-operatively. Results : Mean blood loss, operation time, and admission date showed significant improvements for microscopic lesionectomy compared to traditional lumbar discectomy [P < 0.001]. Also, both measures of short-term functional improvement, the Visual Analogue Scale[VAS] and Roland-Morris[RM] scores, were statistically better for microscopic lesionectomy than for traditional discectomy [P < 0.001]. Conclusion : Posterior microscopic lesionectomy can be performed more safely and provide greater benefit than traditional discectomy. The procedure is associated with less post-operative pain, shorter hospital stays, and quicker rehabilitation.

• ALGAE
• /
• 제21권4호
• /
• pp.479-483
• /
• 2006

In preparation of the biological samples for electron microscopy, the chemical fixation by glutaraldehyde, paraformaldehyde, and OsO4 has been generally used for a long time. However, the chemical fixation method has some problems: the infiltration time is a little bit long and the ultrastructure of cell or tissue transforms before complete fixation of sample. So, recently, cryo-fixation is considered more often in biomedical field. In this study, we compared High Pressure Freezing (HPF) method with chemical fixation method using a algal sample (Ptilota filicina J. Agardh), which was difficult to fix using chemical fixation method. In chloroplast, the ultrastructure of thylakoid lamella and phycobilisome can not show clearly by chemical fixation. In this study we could observe the ultrastructure of thylakoid lamella and phycobilisome of chloroplast very clearly using HPF fixation. An improved images of ultrastructures of nucleus, mitochondrion and floridean starch could obtain. These results suggest that HPF method is very useful method in algal specimen for electron microscopy.

• Applied Microscopy
• /
• 제42권2호
• /
• pp.105-109
• /
• 2012

Electron tomography (ET) of biological specimens is performed from a series of images obtained over a range of tilt angles in a transmission electron microscope. When using the high voltage electron microscope (HVEM), various noises appear in EM images acquired from thick sections by high voltage electron beam. In order to obtain an adequate result in electron tomograms that allow visualization of rather complex and mega-cellular structure such as brain tissue, it is necessary to remove the noise in each original tilt images of thick section. Using band-pass filtering of original tilt images, the filtered images are obtained and used to assemble a reconstructed tomogram. The qualified 3D tomogram from filtered images results in a considerable reduction of the noises compared to conventional tomogram. In conclusion, this study suggests that band-pass filtering is effective to improve the brightness and intensity of HVEM produced tomograms acquired from micron-thick sections of biological specimens.

• 대한화학회지
• /
• 제62권3호
• /
• pp.243-252
• /
• 2018

이 연구에서는 중학교 2학년 학생들의 물질의 특성에 대한 거시적, 미시적 수준의 개념을 비교하였다. 기초 입자 개념 검사와 녹는점, 용해도, 밀도 개념에 대한 거시적, 미시적 수준의 개념 검사를 개발하여 371명의 중학생들을 대상으로 실시하였다. 학생들의 응답을 거시적 수준과 미시적 수준의 개념 이해 여부에 따라 네 가지로 범주화하고 범주별 비율과 특징을 분석하였다. 연구 결과, 거시적 수준의 개념을 이해한 학생들은 미시적 수준에서 다양한 오개념을 가지고 있는 경우가 많았다. 많은 학생들은 물질의 특성과 관련한 미시적 수준의 개념뿐 아니라 거시적 수준의 개념 이해에도 어려움을 겪었다. 거시적 수준의 개념을 이해하지 못한 학생들은 대부분 미시적 수준에서 거시적 수준의 응답과 일치하는 응답을 하지 못하였다. 기초 입자 개념 문항에 옳은 응답을 한 학생들도 물질의 특성 관련 개념을 이해하는 데 어려움을 겪었으나, 거시적 수준의 개념을 이해한 경우 미시적 수준의 개념도 함께 이해한 비율이 높았다. 연구 결과를 바탕으로 물질의 특성 관련 개념의 효과적인 교수학습 및 향후 교육과정의 개선을 위한 교육적 시사점을 논의하였다.

• Bulletin of the Korean Chemical Society
• /
• 제28권3호
• /
• pp.391-396
• /
• 2007

The three-dimensional structure of an inorganic crystal, CaMoO4 (space group I 41/a, a = 5.198(69) A and c = 11.458(41) A), was determined by electron crystallography utilizing a high voltage electron microscope. An initial structure of CaMoO4 was determined with 3-D electron diffraction patterns. This structure was refined by crystallographic image processing of high resolution TEM images. X-ray crystallography of the same material was performed to evaluate the accuracy of the TEM structure determination. The cell parameters of CaMoO4 determined by electron crystallography coincide with the X-ray crystallography result to within 0.033-0.040 A, while the atomic coordinates were determined to within 0.072 A.

• Journal of the Korean Wood Science and Technology
• /
• 제15권1호
• /
• pp.22-55
• /
• 1987

본(本) 연구(硏究)는 육안적(肉眼的) 특성(特性)에 의한 목재식별(木材識別)의 속보(續報)로써 앞으로 생산가능(生産可能)한 한국산(韓國産) 단판수종(單板樹種)의 현미경적(顯微鏡的) 목재특성(木材特性)을 조사(調査)하여 합판공업(合板工業)에 필요(必要)한 기초자료(基礎資料)를 제공하기 위하여 실시(實施)하였다. 선정(選定)된 50수종(樹種)의 현미경적(顯微鏡的) 특성(特性)을 조사(調査)하여 수종별(樹種別)로 기재(記載)하였고 이를 바탕으로 하여 침엽수재(針葉樹材) 및 활엽수재별(闊葉樹材別)로 목재식별(木材識別) 검색표(檢索表)를 간략(簡略)하게 보고(報告)하면 다음과 같다.

• Tuberculosis and Respiratory Diseases
• /
• 제52권5호
• /
• pp.550-556
• /
• 2002

Microscopic polyangiitis is a systemic small-vessel vasculitis that is associated primarily with necrotizing glomerulonephritis and pulmonary capillaritis. A recurrent and diffuse alveolar hemorrhage due to pulmonary capillaritis is the main clinical manifestation of lung involvement. Recently, and interstitial lung disease that mimics idiopathic pulmonary fibrosis was reported to be rarely associated with microscopic polyangiitis. Here we report two patients with microscopic polyangiitis who showed a honeycomb lung at the time of the initial diagnosis with a brief review of relevant literature.

• Applied Microscopy
• /
• 제37권3호
• /
• pp.199-208
• /
• 2007

본 장치는 실험자가 레이저 빔과 광학 렌즈를 조정하여 이미지 및 회절도형의 형성, 보강 및 소멸간섭과 같은 파동광학 현상을 이해하도록 제작된 실험 장치이다. 실험장치는 광원으로 쓰이는 레이저빔과 빔의 광축을 정렬하는 광원 부분과 시료대, 대물렌즈, 중간렌즈, 확대렌즈, CCD system, 컴퓨터, 그리고 렌즈를 상하 조절하는 경통부분으로 구성된다. 본 장치를 통해서 다양한 회절격자의 이미지 및 회절도형을 최대 약 44배 확대할 수 있고, 최대 약 5um의 분해능을 가지고 분석할 수 있다. 이 장치는 전자현미경 이용자들이 TEM의 원리를 보다 쉽게 이해하는데 도움을 주리라 기대한다.

• Journal of Audiology & Otology
• /
• 제23권3호
• /
• pp.140-144
• /
• 2019

Background and Objectives: Inlay butterfly cartilage tympanoplasty makes the graft easy, and reduces operating time. The present study aimed to investigate the outcomes of microscopic versus endoscopic inlay butterfly cartilage tympanoplasty. Subjects and Methods: In this retrospective study, the outcomes of 63 patients who underwent inlay butterfly cartilage tympanoplasty with small to medium chronic tympanic membrane perforation were evaluated. Twenty-four patients underwent conventional microscopic tympanoplasty and 39 underwent endoscopic tympanoplasty. The outcomes were analyzed in terms of the hearing gain and graft success rate. Results: The surgical success rate was 95.8% in the patients who underwent conventional microscopic tympanoplasty and 92.3% in those who underwent endoscopic tympanoplasty. In both groups of patients, the postoperative air-bone gap (ABG) was significantly lower than the preoperative ABG. There were no significant differences between the preoperative and postoperative ABG values in either group. Conclusions: Endoscopic inlay tympanoplasty using the butterfly cartilage technique appears to be an effective alternative to microscopic tympanoplasty and results in excellent hearing.