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http://dx.doi.org/10.4490/ALGAE.2006.21.4.479

Ultrastructures of Ptilota filicina (Rhodophyta) by High Pressure Freezing(HPF): Comparison of HPF Fixation and Chemical Fixation  

Lee, Sang-Hee (Division of Electron Microscopic Research, Korea Basic Science Institute)
Kim, Youn-Joong (Division of Electron Microscopic Research, Korea Basic Science Institute)
Jeong, Jong-Man (Division of Electron Microscopic Research, Korea Basic Science Institute)
Kim, Jin-Gyu (Division of Electron Microscopic Research, Korea Basic Science Institute)
Kim, Young-Min (Division of Electron Microscopic Research, Korea Basic Science Institute)
Kweon, Hee-Seok (Division of Electron Microscopic Research, Korea Basic Science Institute)
Moon, Won-Jin (KBSI, Gwang-ju Center)
Lee, Seok-Hoon (Division of Electron Microscopic Research, Korea Basic Science Institute)
Publication Information
ALGAE / v.21, no.4, 2006 , pp. 479-483 More about this Journal
Abstract
In preparation of the biological samples for electron microscopy, the chemical fixation by glutaraldehyde, paraformaldehyde, and OsO4 has been generally used for a long time. However, the chemical fixation method has some problems: the infiltration time is a little bit long and the ultrastructure of cell or tissue transforms before complete fixation of sample. So, recently, cryo-fixation is considered more often in biomedical field. In this study, we compared High Pressure Freezing (HPF) method with chemical fixation method using a algal sample (Ptilota filicina J. Agardh), which was difficult to fix using chemical fixation method. In chloroplast, the ultrastructure of thylakoid lamella and phycobilisome can not show clearly by chemical fixation. In this study we could observe the ultrastructure of thylakoid lamella and phycobilisome of chloroplast very clearly using HPF fixation. An improved images of ultrastructures of nucleus, mitochondrion and floridean starch could obtain. These results suggest that HPF method is very useful method in algal specimen for electron microscopy.
Keywords
freeze substitution; High Pressure Freezing; Ptilota filicina;
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