• Korean journal of applied entomology
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• v.49 no.3
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• pp.227-232
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• 2010

Toxicity of seven environment friendly agricultural materials (EFAM), which have been used in the domestic market were evaluated on honeybee (Apis mellifera) and asian multicolored ladybird beetle (Harmonia axyridis). Three EFAMs made from plant extract agents (Wangjoongwang Eco, Bogum Eco and Bestop Eco) and four EFAMs made from microbial utilizing agents (Worldstar Eco, Goodmorning, Bluechip and Cameleon) were investigated as EFAMs. In evaluation of toxicity on honeybee, the $RT_{25}$ values of 3 EFAMs made from plant extract agents ranged from 1 to 3 days. Therefore, honeybee should be released 1-3 days after application of these EFAMs. Meanwhile, the four agricultural materials made from microbial utilizing agents did not show any mortality against honeybee. In evaluating the toxicity to adult and larva ladybird beetles, all seven EFAMs made from plant extract agents and microbial utilizing agents to show any mortality.

• Microbiology and Biotechnology Letters
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• v.41 no.3
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• pp.292-299
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• 2013

In this study, we analyzed the changes in fungal populations of red-pepper fields employing eco-friendly farming methods, such as microbial agents and crop rotation, by using polymerase chain reactions coupled with denaturing gradient gel electrophoresis (PCR-DGGE). Primer specific for fungi were used to determine the contribution of domains to the microbial community. Analysis of planted and non-planted soil samples applying PCR-DGGE technology offered evaluation of long-term patterns in fungal species richness. To evaluate the stability of DGGE patterns from different soils, comparison of planted and non-planted soil samples were compared using PCR-DGGE. The number of DNA fragments obtained from all planted soil samples by DGGE separation was far greater (14 to 15 bands) than that of the non-planted soil samples (3 to 4 bands). In addition, 14 bands were observed from crop continuation soil treated with agrochemicals and 18 bands from crop rotation soil treated with microbial agents. The PCR-DGGE analysis suggests that the use of crop rotation and microbial agents benefits the fungal community more than crop continuation using agrochemicals. These results indicate that crop rotation with microbial agents was better able to support beneficial organisms, enable more effective biological control and maintain a healthier balance of nutrients, organic matter and microorganisms.

• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1278-1283
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• 2007

The 30% resistant frequencies of pathogenic bacteria were identified against generally utilizing disinfectants. Among the used disinfectants, foodsef, Taego, and Iodo 175 were dictated by lower sensitivities against pathogenic bacteria, as well as higher resistant frequencies when compared with other disinfectants. The resistant frequencies against antibiotics were also dictated by 30% through MIC (minimal inhibitory concentration) and paper disc methods. Especially, the used bacteria exhibited resistances against gentamycin, kanamycin, and streptomycin, which included in all aminoglycoside group. The MBT-01108 material, which extracted and purified from a powder obtained by processing of Opunita ficus-indica var. saboten Makino trunk, did not develop or grow resistant bacteria. Interestingly, the multi-drug resistant bacteria such as MRSA, resistant Pseudomonas aeruginosa, VRE, and E. coli 0157 did not resistant against MBT-01108 material. These results suggest that MBT-01108 material uses as an anti-microbial agent.