• Journal of Microbiology and Biotechnology
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• v.32 no.6
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• pp.681-698
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• 2022

The Nile tilapia Oreochromis niloticus, Atlantic salmon Salmo salar, rainbow trout Oncorhynchus mykiss, olive flounder Paralichthys olivaceus, common carp Cyprinus carpio, grass carp Ctenopharyngodon idella and rohu carp Labeo rohita are farmed commercially worldwide. Production of these important finfishes is rapidly expanding, and intensive culture practices can lead to stress in fish, often reducing resistance to infectious diseases. Antibiotics and other drugs are routinely used for the treatment of diseases and sometimes applied preventatively to combat microbial pathogens. This strategy is responsible for the emergence and spread of antimicrobial resistance, mass killing of environmental/beneficial bacteria, and residual effects in humans. As an alternative, the administration of probiotics has gained acceptance for disease control in aquaculture. Probiotics have been found to improve growth, feed utilization, immunological status, disease resistance, and to promote transcriptomic profiles and internal microbial balance of host organisms. The present review discusses the effects of single and multi-strain probiotics on growth, immunity, heamato-biochemical parameters, and disease resistance of the above-mentioned finfishes. The application and outcome of probiotics in the field or open pond system, gaps in existing knowledge, and issues worthy of further research are also highlighted.

• Fisheries and Aquatic Sciences
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• v.25 no.9
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• pp.463-472
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• 2022

In this study, a microbial risk assessment was performed for the bacteria Vibrio parahaemolyticus, which causes a foodborne illness following the consumption of Jeotgal, a fermented seafood in South Korea. The assessment comprised of six stages: product, market, home, consumption, dose-response, and risk. The initial contamination level (IC) was calculated based on the prevalence of V. parahaemolyticus in 90 Jeotgal samples. The kinetic behavior of V. parahaemolyticus was described using predictive models. The data on transportation conditions from manufacturer to market and home were collected through personal communication and from previous studies. Data for the Jeotgal consumption status were obtained, and an appropriate probability distribution was established. The simulation models responding to the scenario were analyzed using the @RISK program. The IC of V. parahaemolyticus was estimated using beta distribution [Beta (1, 91)]. The cell counts during transportation were estimated using Weibull and polynomial models [δ = 1 / (0.0718 - 0.0097 × T + 0.0005 × T²)], while the probability distributions for time and temperature were estimated using Pert, Weibull, Uniform, and LogLogistic distributions. Daily average consumption amounts were assessed using the Pareto distribution [0.60284,1.32,Risk Truncate(0,155)]. The results indicated that the risk of V. parahaemolyticus infection through Jeotgal consumption is low in South Korea.

• Journal of Environmental Science International
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• v.30 no.5
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• pp.379-389
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• 2021

Soil microorganism activity in an agricultural field is affected by various factors including climate conditions, soil chemical properties, and crop cultivation. In this study, we elucidate the correlation between microorganism activity and agricultural environment factors using the dehydrogenase activity (DHA) value, which is one of the indicators of soil microbial activity. As a result, the various factors noted above were related to the DHA value. Annual rainfall, soil Mg2+, bacterial and fungal diversities, types of crops, developmental stages, seasons, and cultivation status were highly correlated with the DHA value. Furthermore, next-generation sequencing (NGS) analysis was used to identify that the type of crop affected soil microbial compositions of both bacteria and fungi. Soil used for soybean cultivation showed the highest relative abundance for Verrucomicrobia, Planctomycetes, and Acidobacteria but Actinobacteria and Firmicutes had the lowest relative abundance. In the case of soil used for potato cultivation, Actinobacteria had the highest relative abundance but Proteobacteria had the lowest relative abundance. Armatimonadetes showed the highest relative abundance in soil used for cabbage cultivation. Among the fungal communities, Mortierellomycota had the highest relative abundance for soybean cultivation but the lowest relative abundance for cabbage cultivation; further, Rozellomycota, Chytridiomycota, and Cercozoa had the highest relative abundance for cabbage cultivation. Basidiomycota had the highest relative abundance for potato cultivation but the lowest relative abundance for soybean cultivation.

• Journal of Animal Science and Technology
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• v.64 no.6
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• pp.1092-1104
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• 2022

Using antibiotics as growth promoter has been banned in poultry feed industry, thus various researchers try to seek an alternative to replace the growth-promoting antibiotics. In this study, we aimed to evaluate the growth performance via intestinal nutrient utilization and cecal microbial composition of broiler after dietary supplementation with most commonly using antibiotics, zinc bacitracin, and sophorolipid. A total of 180 1-day-old chicks were randomly assigned, and dietary treatment was as follow: CON, basal diet; ZB, 100 ppm of zinc bacitracin supplemented diet; and SPL, 250 ppm of sophorolipid supplemented diet. Their growth performance was evaluated and the samples of blood, small intestine, and ileal and cecal digesta were collected for biochemical, histological, and genomic analyses. The body weight and average daily gain of 7-day-old chicks were higher in ZB and those in overall experimental period were improved by ZB and SPL supplementation (p < 0.05). Their intestinal characteristics were not affected by dietary treatments in duodenum and ileum. Nonetheless, villus height was increased by SPL supplementation in jejunum (p < 0.05). Moreover, dietary SPL supplementation could down-regulate the expression level of pro-inflammatory cytokine, IL-1β (p < 0.05). mRNA levels of lipid and protein transporters did not differ among the treatments, however, relative expression levels of carbohydrate transporters, GLUT2 and SGLT1 were increased in broiler chicken's jejumum fed zinc bacitracin and sophorolipid supplemented diets (p < 0.05). Dietary zinc bacitracin supplementation could increase the population of Firmicutes in phylum level, and the portion of Turiciacter in genus level. On the other hands, the portion of Faecalibacterium was increased by dietary SPL supplementation compared to the other treatments. Our findings suggest that SPL supplementation improves growth performance through enhanced carbohydrate utilization capacity via improvement of gut morphological status and modulation of the cecal microbial population of broilers.

• The Korean Journal of Food And Nutrition
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• v.36 no.3
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• pp.193-201
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• 2023

Prebiotics are known as components of intestinal microbiota that can improve and maintain human health status by stimulating the growth and activity of the intestinal tract as a method of controlling the intestinal environment. In this study, we examined whether 2'-fucosyllactose (FL) could affect intestinal microbial population and bowel activity. Water content and frequency of mouse feces were increased in the 2'-FL treated group at a high concentration (1,000 mg/kg), with brightness of the color enhanced and physical properties diluted. In addition, intestinal microbial analysis showed that harmful bacteria Clostridium and Staphylococcus strains were decreased and beneficial bacteria such as Lactobacillus strains were markedly increased in the group treated with a high concentration of 2'-FL compared to those in the control group. These findings suggest that administration of 2'-FL can maintain healthy bowel activity by reducing harmful bacteria population and improving diluted physical properties.

• Journal of Genetic Medicine
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• v.20 no.2
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• pp.52-59
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• 2023

Purpose: Colorectal cancer (CRC) is a common malignancy worldwide and the second leading cause of cancer-related deaths. In addition, lymph node metastasis in CRC is considered an important prognostic factor for predicting disease recurrence and patient survival. Recent studies demonstrated that the microbiome makes substantial contributions to tumor progression, however, there is still unknown about the microbiome associated with lymph node metastasis of CRC. Here, we first reported the microbial and tumor-infiltrating immune cell differences in CRC according to the lymph node metastasis status. Materials and Methods: Using Next Generation Sequencing data acquired from 368 individuals diagnosed with CRC (N0, 266; N1, 102), we applied the LEfSe to elucidate microbial differences. Subsequent utilization of the Kaplan-Meier survival analysis enabled the identification of particular genera exerting significant influence on patient survival outcomes. Results: We found 18 genera in the N1 group and 3 genera in the N0 group according to CRC lymph node metastasis stages. In addition, we found that the genera Crenobacter (P=0.046), Maricaulis (P=0.093), and Arsenicicoccus (P=0.035) in the N0 group and Cecembia (P=0.08) and Asanoa (P=0.088) in the N1 group were significantly associated with patient survival according to CRC lymph node metastasis stages. Further, Cecembia is highly correlated to tumor-infiltrating immune cells in lymph node metastasized CRC. Concolusion: Our study highlights that tumor-infiltrating immune cells and intratumoral microbe diversity are associated with CRC. Also, this potential microbiome-based oncology diagnostic tool warrants further exploration.

• Korean Journal of Veterinary Research
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• v.51 no.1
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• pp.47-53
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• 2011

In Korea, groundwater is main water source in livestock farms. Most dairy and cattle farms have constructed their own wells for human drinking and livestock farming. However, these private residential wells have not been controlled by government and also there was scant study about livestock drinking water quality. Therefore this study was to monitor of the livestock farms' groundwater quality in Korea. Water samples were collected at 123 dairy and cattle farms and were analysed forty six substances with quality standard for drinking water approved by the Minister of Environment. Seventy eight (63.4%) of 123 samples failed to drinking water stand a test. The most frequent contaminants were nitrate-nitrogen and microbial. 22.8% (n=28) of samples showed nitrate-N concentration of higher than 10 mg/L meant that can't be used drinking water for human and the Nitrate-N concentration analysed in the range of 0.2 to 61.2 mg/L. All of 78 failed to drinking samples had microbial problems, especially 5.7% (n=7) of samples indicated water could be contaminated by feces. Other contaminants detected were zinc and evaporation residue. Especially detected zinc concentration (32 mg/L) was about ten times higher than standard of zinc (3 mg/L). Regression analysis indicated that groundwater pH did not influence to nitrate-N concentration but the hardness and chloride could affect to nitrate-N concentration in the groundwater. Most livestock farms were adjacent to crop farmland in Korea. This could cause contamination of groundwater with nitrate-N and pesticide that could accumulate livestock product. Moreover Heavy metal such as zinc and copper could be released from a corrosive plated water pipe in livestock farm. Put together, Korea livestock system is indoor, not pasture-based, hence livestock could be exposed to potential contaminated water consistently. Therefore on the basis of these data, appropriate livestock drinking water quality standards should be prepared to keep livestock healthy and their product safe. Further, livestock drinking water quality should be monitored continuously in suitable livestock drinking water standards.

• Korean Journal of Veterinary Service
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• v.34 no.4
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• pp.409-416
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• 2011

This study was performed to evaluate the microbial contamination level of meat processing in butcher's shops in Seoul from January to November in 2010. A total of 695 samples (112 cotton work gloves, 342 utensils and equipments, 241 meat samples) were collected and the environmental hygiene of 89 butcher's shops was investigated. The aerobic plate count (APC), E. coli and pathogenic bacteria such as Staphylococcus aureus, Salmonella spp. were tested in the samples. As a result, the level of count on APC ranged $0{\sim}2.2{\times}10^6\;CFU/cm^2$ from the utensils & equipments, $1{\times}10^2{\sim}2.7{\times}10^8$ CFU/glove from the cotton work gloves and $1{\times}10^2{\sim}9.3{\times}10^6$ CFU/g from the meat samples. The level of count on E. coli ranged $0{\sim}2.5{\times}10^3\;CFU/cm^2$ from the utensils, $0{\sim}8.6{\times}10^4$ CFU/glove from the cotton work gloves and $0{\sim}1.4{\times}10^5$ CFU/g from the meat samples, respectively. Staphylococcus aureus and Salmonella spp. were detected in 11 samples and 2 samples from the cotton work gloves, utensils & equipments, respectively. The sanitation standard operating procedure (SSOP) was applicated at 49 butcher's shops. In order to improve sanitation of meat in butcher's shops, in this study, applications of SSOP, systemic sanitation education for employees, hygenic control of utensils & equipments, and continuous monitoring for microorganisms will be required.

• Environmental Engineering Research
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• v.20 no.1
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• pp.1-18
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• 2015

The biosorption process has been established as characteristics of dead biomasses of both cellulosic and microbial origin to bind metal ion pollutants from aqueous suspension. The high effectiveness of this process even at low metal concentration, similarity to ion exchange treatment process, but cheaper and greener alternative to conventional techniques have resulted in a mature biosorption technology. Yet its adoption to large scale industrial wastewaters treatment has still been a distant reality. The purpose of this review is to make in-depth analyses of the various aspects of the biosorption technology, staring from the various biosorbents used till date and the various factors affecting the process. The design of better biosorbents for improving their physico-chemical features as well as enhancing their biosorption characteristics has been discussed. Better economic value of the biosorption technology is related to the repeated reuse of the biosorbent with minimum loss of efficiency. In this context desorption of the metal pollutants as well as regeneration of the biosorbent has been discussed in detail. Various inhibitions including the multi mechanistic role of the biosorption technology has been identified which have played a contributory role to its non-commercialization.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.5
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• pp.483-493
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• 1996

Advances in silage technology, including precision chop forage harvesters, improved silos, polyethylene sheeting, shear cutting silo unloaders, and the introduction of total mixed rations, have made silage the principal method of forage preservation. A better understanding of the biochemistry and microbiology of the four phases of the ensiling process has also led to the development of numerous silage additives. Although acids and acid salts still are used to ensile low-DM forages in wet climates, bacterial inoculants have become the most widely used silage additives in the past decade. Commercial inoculants can assure a rapid and efficient fermentation phase; however, in the future, these products also must contribute to other areas of silage management, including the inhibition of enterobacteria, clostridia, and yeasts and molds. Nonprotein nitrogen additives have the problems of handling, application, and reduced preservation efficiency, which have limited their wide spread use. Aerobic deterioration in the feedout phase continues to be a serious problem, especially in high-DM silages. The introduction of competitive strains of propionic acid-producing bacteria, which could assure aerobically stable silages, would improve most commercial additives. New technologies are needed that would allow the farmer to assess the chemical and microbial status of the silage crop on a given day and then use the appropriate additive(s).