• The Korean Journal of Community Living Science
• /
• v.21 no.1
• /
• pp.139-148
• /
• 2010

This study was conducted to restore our traditional Cheongtaejeon tea and to develop the special products. We inoculated Cheongtaejeon tea with lactobacilli (Lactobacillus plantarum CHO 25) and the mixed microbial strains (L. plantarum CHO 25 + Saccharomyces cerevisiae + Bacillus amyloliquefaciens CHO 104). We also examined the sensuous characteristics and physiological activity of Cheongtaejeon tea which was produced by the inoculation of microbial strains. The external appearance of Cheongtaejeon teas were not significant among the teas which were produced with or without the inoculation of L. plantarum CHO 25 and the mixed microbial strains. The taste of the tea increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains. The taste and liking of Cheongtaejeon tea which was inoculated with Aspergillus oryzae and Aspergillus niger decreased significantly, and it was not suitable to drink. Total phenolics compound contents, total flavonoid contents and DPPH ($\alpha,\alpha$-diphenyl-$\beta$-picryl-hydrazyl) radical scavenging activity of Cheongtaejeon tea extracts increased much more in the order of that produced with the inoculation of L. plantarum CHO 25, control and that with the mixed microbial strains. However, nitrite radical scavenging activity in 1,000 mg/L Cheongtaejeon tea hot water extracts were in the order of the control (94.4%), the inoculation of L. plantarum CHO 25 (93.6%) and the mixed microbial strains (91.1%). Overall results indicated that the sensuous characteristics increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains, and those physiological activities in tea with the inoculation of L. plantarum CHO 25.

• Korean Journal of Pharmacognosy
• /
• v.23 no.3
• /
• pp.142-145
• /
• 1992

MeOH extract of twenty medicinal herbs were screened for their effects against protein kinase C (PKC) using bleb-forming assay and PKC enzyme assay. Smilax china and Sanguisorba officinalis showed potent anti-PKC activity. Campsis grandiflora and Galla Halepensis showed moderate inhibitory effect on PKC.

• Archives of Pharmacal Research
• /
• v.12 no.4
• /
• pp.236-238
• /
• 1989

The anti-microbial and anti-inflammatory activities of the constituents from Atractylodes rhizomes were evaluated. Atracylone showed anti-microbial activity. Atractylone and attractylenolide I possessed considerable anti-inflammatory activity utilizing rat cotton pellet granuloma bioassay.

• Journal of Korean Society on Water Environment
• /
• v.27 no.2
• /
• pp.188-193
• /
• 2011

The effects of coagulants on the microorganisms when they are injected directly into the biological treatment facility for T-P removal have been easily observed from the results of past experiments. As such this study is set out to derive the effective plans for the coagulant dosage by analyzing the effects of the injected coagulant on the microbial activity during the chemical treatment for T-P removal. The research methods entailed the assessment of removal efficiency of T-P according to the coagulant dosage while changing the molar ration between Alum and influent phosphorus. At the same time Specific Oxygen Uptake Rate (SOUR) according to the coagulant dosage was measured. SOUR was used as a method for indirect assessment of the microbial activity according to the coagulant dosage. The results from the study showed that with the increase in the alum dosage, the removal efficiency T-P tended to increase. On the other hand, the increase in coagulant dosage resulted in the decrease in SOUR, which indicates the decrease in the microbial activity. Such reduction in the activity could be explained by the increase in the concentration of removal efficiency of $TBOD_5$. Based on experiment results from the study, it is determined that coagulant dosage affects the microbial activity. Moreover, the indirect assessment on the microbial activity using SOUR is considered possible.

• Korean Journal of Microbiology
• /
• v.23 no.2
• /
• pp.147-156
• /
• 1985

Seasonal and vertical variations of abiontic soil carboxymethylcellulase (CMCase) activities were assessed every other month for a year in two contrasting forest soils and evaluated the relationships between soil CMCase activity and environmental parameters. In climax deciduous soil, variations in CMCase activities caused by differences in sampling time were greater than those caused by differences in soil depth. On the other hand, counter phenomenon was obserned in coniferous soil at the stage of development. Correlation analyses showed that soil CMCase activities were significantly (p>0.01) correlated with microbial respiration rates ($O_2$ uptake) and all of the microbial population sizes. From these results, it is suggested that determination of abiontic soil CMCase activity is an useful additional index for evaluating the overall microbial growth and activity in soils.

• Journal of Ecology and Environment
• /
• v.34 no.4
• /
• pp.401-410
• /
• 2011

We assayed the effects of simulated acid rain on the mass loss, $CO_2$ evolution, dehydrogenase activity, and microbial biomass-C of decomposing Sorbus alnifolia leaf litter at the microcosm. The dilute sulfuric acid solution composed the simulated acid rain, and the microcosm decomposition experiment was performed at 23$^{\circ}C$ and 40% humidity. During the early decomposition stage, decomposition rate of S. alnifolia leaf litter, and microbial biomass, $CO_2$ evolution and dehydrogenase activity were inhibited at a lower pH; however, during the late decomposition stage, these characteristics were not affected by pH level. The fungal component of the microbial community was conspicuous at lower pH levels and at the late decomposition stage. Conversely, the bacterial community was most evident during the initial decomposition phase and was especially dominant at higher pH levels. These changes in microbial community structure resulting from changes in microcosm acidity suggest that pH is an important aspect in the maintenance of the decomposition process. Litter decomposition exhibited a positive, linear relationship with both microbial respiration and microbial biomass. Fungal biomass exhibited a significant, positive relationship with $CO_2$ evolution from the decaying litter. Acid rain had a significant effect on microbial biomass and microbial community structure according to acid tolerance of each microbial species. Fungal biomass and decomposition activities were not only more important at a low pH than at a high pH but also fungal activity, such as $CO_2$ evolution, was closely related with litter decomposition rate.

• Journal of Microbiology and Biotechnology
• /
• v.6 no.3
• /
• pp.213-218
• /
• 1996

Hot-water extract, Fr. 1, of Phellinus linteus mycelium was fractionated into Fr. 2, 3, 4, and 5 by the difference of solubility in ethanol. The polysaccharide fractions were studied for their immunostimulating activity on in vitro T-independent polyc1onal antibody response to trinitrophenyl-haptened SRBC (sheep red blood cell). The Fr. 4 with the highest immunostimulating activity was subjected to DEAE-cellulose ion exchange chromatography and gave five fractions, 4-I, II, III, IV, and V. The in vitro immunostimulating assay of the five fractions showed that 4-I and 4-III had a similar activity to that of LPS but the other fractions had low activity. By analyses of chemical composition and HPLC, all fractions obtained were found to be heteropolysaccharide-protein complex. The molecular weights ranged from 9, 000 to 15, 000. Sugar analyses showed that glucose, galactose, mannose, arabinose, and xylose were main component. Uronic acid and amino sugar were also detected in the fractions. It should be noted that the molecular weight (15, 000) of 4-III was very small and the structure of 4-III may be different from the known immunostimulating branched $\beta$-(1longrightarrow3)-glucan.

• Journal of Environmental Health Sciences
• /
• v.34 no.3
• /
• pp.207-212
• /
• 2008

This study was performed to investigate the anti-microbial activity of extract from Korean fermented soybean paste (doen-jang) against 16 types of oral microbes, and to determine the minimum inhibition concentration (MIC) of the extract for three major microbes causing human oral diseases (Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans). The extract was prepared using ethyl acetate and it was treated with the oral microbes at a concentration of 5.00 mg/ml (0.5%). The anti-microbial activity and MIC were measured using broth dilution method. Significant reduction of microbial activities of 16 types of oral microbes occurred when the soybean paste extract was added to the broth compared to the control (p<0.01), and striking inhibition (more than 99%) was observed in ten types. S. mutans, which causes dental caries, showed MIC at a concentration of 1.25 mg/ml for the extract. P. gingivalis, which causes adult periodontal disease, showed MIC at a concentration of 2.50 mg/ml for the extract. C. albicans, which causes denture stomatitis and angular stomatitis, showed MIC at a concentration of 20 mg/ml for the extract. These results indicate that ethyl acetate extract of doen-jang showed strong anti-microbial effect against 16 types of oral microbes, and the anti-microbial effect of the extract against oral microbes was stronger against bacteria than against fungi. The anti-microbial effect might be possibly enhanced by the fermentation of soybeans.

• The Korean Journal of Ecology
• /
• v.16 no.2
• /
• pp.191-198
• /
• 1993

The relationships between microbial activity and disturbance level of soil were investigated from 15 parks in Seoul and undisturbed area. The physico-chemical characteristics of soil and dehydrogenase activity(DHA) as an index of soil microbial activity were analysed. There were ranges of 3.84~7.37 in pH, 9.63~40.33% in moisture content, 3.41~21.49% in organic matter, 0.36~0.79g/g in water holding capacity and 0.03~0.53% in total nitrogen investigated sites. DHA values of soil were 8.64~$146.76{\mu}g/g$ in park soil and 545.14~$1, 198.80{\mu}g/g$ in undisurbed area. DHA of park soil with high traffic density and contamination source from human activities was much lower than that of undisturbed area. DHA was positively correlated with moisture content, organic matter, water holding capacity and total nitrogen.

• The Korean Journal of Ecology
• /
• v.15 no.3
• /
• pp.267-279
• /
• 1992

The relationship between pb, zn, cd, and the microbial biomass and activity were investigated in three public park soils of central and outer london. Variability with distance from the roadside and profile were studied. The heavey metal concentrations were the highest in hampstead heath and hyde park with high trafic density and the lowest in hainault. The highest concentrations of heavy metals were found adjacent to the roadside in the upper parts of the soil profile. Dehydrogenase activity, adenosine tri-phosphate and ergosterol contents used as indices of micrbial biomass and activity, were generally higher in hainadult, and also higher in the upper pats of the soil profile. Simple regression analysis indicated that the microbial biomass and activity were affected significantly by moisture content, water holding capacity, total organic carbon, total nitrogen, and organic mater rather than heavy metal concentration. Highest inputs of nitrogen and carbon were associated with high inputs of heavey metals, all three being derived from vehicle emissions adjacent to the road. The hyde park and hampstead heath microbial populations were able to respond to the c and n input positively by increase in biomass and activity, whereas the hainault populations could not. This rsult suggrsts adaptation in he former to heavy matals, but not in the latter.