• 식품기술
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• 제22권1호
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• pp.117-126
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• 2009

• 한국지역사회생활과학회지
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• 제21권1호
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• pp.139-148
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• 2010

This study was conducted to restore our traditional Cheongtaejeon tea and to develop the special products. We inoculated Cheongtaejeon tea with lactobacilli (Lactobacillus plantarum CHO 25) and the mixed microbial strains (L. plantarum CHO 25 + Saccharomyces cerevisiae + Bacillus amyloliquefaciens CHO 104). We also examined the sensuous characteristics and physiological activity of Cheongtaejeon tea which was produced by the inoculation of microbial strains. The external appearance of Cheongtaejeon teas were not significant among the teas which were produced with or without the inoculation of L. plantarum CHO 25 and the mixed microbial strains. The taste of the tea increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains. The taste and liking of Cheongtaejeon tea which was inoculated with Aspergillus oryzae and Aspergillus niger decreased significantly, and it was not suitable to drink. Total phenolics compound contents, total flavonoid contents and DPPH ($\alpha,\alpha$-diphenyl-$\beta$-picryl-hydrazyl) radical scavenging activity of Cheongtaejeon tea extracts increased much more in the order of that produced with the inoculation of L. plantarum CHO 25, control and that with the mixed microbial strains. However, nitrite radical scavenging activity in 1,000 mg/L Cheongtaejeon tea hot water extracts were in the order of the control (94.4%), the inoculation of L. plantarum CHO 25 (93.6%) and the mixed microbial strains (91.1%). Overall results indicated that the sensuous characteristics increased most in Cheongtaejeon tea which was produced without the inoculation of microbial strains, and those physiological activities in tea with the inoculation of L. plantarum CHO 25.

• 한국미생물·생명공학회지
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• 제24권1호
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• pp.19-26
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• 1996

A phospholipase C (PLC) inhibitor (MT267-2B) was isolated from the culture broth of actinomycetes isolate MT2617-2 by the extraction with n-butanol and column chromatographic techniques. The molecular weight of the inhibitor was 1057, by the spectroscopic analyses of IR, $^{13}C$-and $^{1}H$-NMR and ESI-MS. The chemical structure of MT2617-2B was found to be a macrolide compound consisted of a hemiketal ring, polyhydroxyl and polymethyl groups, which had a malonate and guanidine group as its side chain. MT2617-2B produced its two isomers having the same molecular weight by standing in methanol solution at room temperature. Therefore, MT2617-2B was identified as copiamycin and niphithricin A, macrolide antibiotics. The values of $IC_{50}$ against PLC-${\gamma}$1 and PLC-${\beta}$1 were 25 and 50${\mu}$g/ml, respectively. MT2617-2B had antimicrobial activities against Staphylococcus aureus and Candida albicans, but not against Escherichia coli.

• 한국환경보건학회지
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• 제34권3호
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• pp.207-212
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• 2008

This study was performed to investigate the anti-microbial activity of extract from Korean fermented soybean paste (doen-jang) against 16 types of oral microbes, and to determine the minimum inhibition concentration (MIC) of the extract for three major microbes causing human oral diseases (Streptococcus mutans, Porphyromonas gingivalis, and Candida albicans). The extract was prepared using ethyl acetate and it was treated with the oral microbes at a concentration of 5.00 mg/ml (0.5%). The anti-microbial activity and MIC were measured using broth dilution method. Significant reduction of microbial activities of 16 types of oral microbes occurred when the soybean paste extract was added to the broth compared to the control (p<0.01), and striking inhibition (more than 99%) was observed in ten types. S. mutans, which causes dental caries, showed MIC at a concentration of 1.25 mg/ml for the extract. P. gingivalis, which causes adult periodontal disease, showed MIC at a concentration of 2.50 mg/ml for the extract. C. albicans, which causes denture stomatitis and angular stomatitis, showed MIC at a concentration of 20 mg/ml for the extract. These results indicate that ethyl acetate extract of doen-jang showed strong anti-microbial effect against 16 types of oral microbes, and the anti-microbial effect of the extract against oral microbes was stronger against bacteria than against fungi. The anti-microbial effect might be possibly enhanced by the fermentation of soybeans.

• 한국토양비료학회지
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• 제43권6호
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• pp.1012-1017
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• 2010

본 연구는 고추 연작의 작부체계가 토양 미생물의 변화에 미치는 효과를 분석하고자 10년 동안 무농약 및 화학적 무비료의 시용방법으로 수행되었다. 지표 PLFAs 분석을 통해 토양미생물상과 활성도를 평가하였다. 고추와 마늘의 단작, 마늘과 고추의 간작의 작부체계에 따른 토양의 화학학성은 큰 차이를 보이지 않았으나 토양 미생물의 PLFAs의 다양성은 PC2에서 다른 작부체계와 상이한 미생물상으로 구분되었으며, 토양 미생물의 PLFAs의 총량은 마늘 단작구와 비교하여 고추 단작구에서 유의적인 감소를 보였으며, 미생물군집의 구조는 내생균근균의 비율에서 유의성있는 감소를 보였다. 이는 마늘과 고추의 근계에 따라 내생균근균의 미생물체량의 감소하였으며, 이것이 토양미생물상과 활성도를 다른 작부체계와 구분한 것으로 판단된다.

• 한국토양비료학회지
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• 제42권6호
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• pp.500-512
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• 2009

유기물원을 항온 배양했을 때 온도가 토양미생물체량과 효소활성 및 PLFA함량에 미치는 영향을 토양 특성별로 평가하고자 입상과 분상 혼합유기질비료, 돈분퇴비, 음식물퇴비를 화산회토양과 비화산회토양 30g에 2g을 잘 혼합 후 10, 20, $30^{\circ}C$에서 항온배양을 하면서 pH, 토양질소, 유기물 함량, Microbial biomass C와 N, 토양효소활성, 인지질지방산 함량을 분석하였다. 돈분과 음식물퇴비는 토양종류와 온도에 상관없이 토양 pH의 변화가 크지 않았으나 입상유기질비료는 온도가 높을수록 낮아졌으며, 비화산회토양에서 변화폭이 컸다. 미생물체량 C는 화산회토양의 경우 배양 온도가 높고 유기질비료를 처리할 때 높아지는 경향이었지만 토양종류에 상관없이 점차 감소하였다. 미생물체량 N은 비화산회토양에서 유기질비료, 화산회토양에서는 돈분과 음식물퇴비를 처리할 때 높게 나타났다. 인지질 지방산함량은 항온배양 75일후 비화 산회토양이 화산회토양보다 높았고, 270일후 화산회토양에서 높게 나타났으나 점차 낮아지는 경향이었다. Urease활성은 150일에 비화산회토양의 입상유기질비료처리에서 $10^{\circ}C$(75.0)>$20^{\circ}C$(16.3)>$30^{\circ}C$($4.6ug\;NH{_4-}N\;g^{-1}\;2h^{-1}$)순으로 $10^{\circ}C$에서 가장 높게 나타났으며 온도가 높고, 시간이 경과할수록 낮아졌으며 화산회토양의 Urease활성은 $10^{\circ}C$에서 높게 나타났다. ${\beta}-glucosidase$ 활성은 비화산회토양이 화산회토양보다 높았고 시간이 경과할수록 낮아졌다. 토양미생물체량 C와 미생물활성지표간의 상관계수는 비화산회토양에서 PLFA ($r^2=0.91$), 화산회토양에서 ${\beta}-glucosidase$ ($r^2=0.83$)가 높았으며, 미생물활성지표는 토양종류와 온도에 따라 상대적으로 민감도가 다르게 나타났다.

• 한국식품영양과학회지
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• 제45권5호
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• pp.778-781
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• 2016

본 연구에서는 폐기되는 성게 껍질을 이용한 생분해성 필름소재를 개발하고자 gelatin을 첨가한 복합필름 및 항산화, 항균성을 부여하기 위해 아까시 꽃 추출물(RFE)을 첨가한 필름을 제조하였다. 그 결과 gelatin 비율이 증가함에 따라 성게 껍질 필름의 인장강도가 증가하였고 신장률이 감소하였으며, 성게 껍질과 gelatin을 8:2 비율로 첨가하였을 때 최적의 물성을 보였다. 또한, 필름에 RFE를 첨가함에 따라 인장강도는 감소하였으나 신장률과 필름 투습도는 증가하였다. RFE 함유 성게 껍질 필름의 ABTS, DPPH 라디칼 소거능 측정과 디스크 확산법을 이용하여 항산화 및 항균 능력을 측정하였는데, RFE 농도가 증가함에 따라 항산화, 항균 능력이 향상하였다. 따라서 본 연구 결과, RFE를 첨가한 생분해 성게 껍질 필름은 적절한 물성과 함께 항산화, 항균 능력이 있기에 가공식품의 저장성을 증대시킬 수 있는 기능성 포장 소재로의 활용이 가능하다고 판단된다.

• Journal of Microbiology and Biotechnology
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• 제24권6호
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• pp.843-853
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• 2014

In the context of artificial groundwater recharge, a reactive soil column at pilot-scale (4.5 m depth and 3 m in diameter) fed by treated wastewater was designed to evaluate soil filtration ability. Here, as a part of this project, the impact of treated wastewater filtration on soil bacterial communities and the soil's biological ability for wastewater treatment as well as the relevance of the use of multi-bioindicators were studied as a function of depth and time. Biomass; bacterial 16S rRNA gene diversity fingerprints; potential nitrifying, denitrifying, and sulfate-reducing activities; and functional gene (amo, nir, nar, and dsr) detection were analyzed to highlight the real and potential microbial activity and diversity within the soil column. These bioindicators show that topsoil (0 to 20 cm depth) was the more active and the more impacted by treated wastewater filtration. Nitrification was the main activity in the pilot. No sulfate-reducing activity or dsr genes were detected during the first 6 months of wastewater application. Denitrification was also absent, but genes of denitrifying bacteria were detected, suggesting that the denitrifying process may occur rapidly if adequate chemical conditions are favored within the soil column. Results also underline that a dry period (20 days without any wastewater supply) significantly impacted soil bacterial diversity, leading to a decrease of enzyme activities and biomass. Finally, our work shows that treated wastewater filtration leads to a modification of the bacterial genetic and functional structures in topsoil.

• 한방안이비인후피부과학회지
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• 제20권2호통권33호
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• pp.10-35
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• 2007

Objectives : To investigate the effects of Ohbaesangami (OBSGM) on mucosa and skin diseases, anti-microbial and anti-inflammatory tests were performed using several in vitro test models. Results : In anti-microbial test, OBSGM showed the slight inhibitory effect against Propionibacterium acnes (P. acnes) and Staphylococcus aureus (S. aureus). In anti-oxidant test, OBSGM showed the potent radical scavenging activity. In anti-inflammatory test, OBSGM weakly inhibited the lipopolysaccharide (LPS)-induced nitric oxide(NO) release from the RAW 264.7 macrophage cells. OBSGM also inhibited the LPS-induced $interleukin-1{\beta}(IL-1{\beta})$ and cyclooxygenase-2 (COX-2) expressions. The inhibitory effects of OBSGM on macrophage activation was via the inhibition of $NF-{\kappa}B$, evidenced by transient transfection assay. Furthermore, OBSGM markedly inhibited the activation of Jun-N-terminal kinase (JNK) and p38 MAP kinase in RAW 264.7 cells. In skin wrinkle formation assay, OBSGM strongly inhibited collagnease and elastase, whose activities are tightly related with the wrinkle formation. In addition, OBSGM inhibited the activities of MMP-1, MMP-2 on the mRNA levels in RAW 264.7 cells. However, OBSGM did not show an inhibitory potential on tyrosinase activity and melanin synthesis, indicating that it could not be applicable for skin whitening. Conclusion : These results suggest that the anti-inflammatory effect of OBSGM may be due to its inhibitory potentials on the macrophage activation. And, the anti-wrinkle effects of OBSGM may be due to its inhibitory potential on the collagnease and elastase activities. Therefore, OBSGM could be applicable for the treatment of mucosa and skin diseases.

• 한국환경과학회지
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• 제19권1호
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• pp.17-26
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• 2010

To understand the initial changes in the microbial activities of wetland soil after construction, dehydrogenase activity (DHA) and denitrification potential (DNP) of soil from 1 natural wetland and 2 newly constructed wetlands were monitored. Soil samples were collected from the Daepyung marsh as a natural wetland, a treatment wetland in the West Nakdong River, and an experimental wetland in the Pukyong National University, Busan. The results showed that the DHA of the natural wetland soil was 6.1 times higher than that of the experimental wetland and similar to that of the treatment wetland 6 months after wetland construction (fall). Few differences were observed in the DNP between the soil samples from the natural wetland and 2 constructed wetlands four months after wetland construction (summer). However, 6 months after the construction (fall), the DNP of the soil samples from the natural wetland was 12.9 times and 1.8 times higher than that of the experimental wetland and the treatment wetland, respectively. These results suggested that the presence of organic matter as a carbon source in the wetland soil affects the DHA of wetland soil. Seasonal variation of wetland environment, acclimation time under anaerobic or anoxic wetland conditions, and the presence of carbon source also affect the DNP of the wetland soil. The results imply that the newly constructed wetland requires some period of time for having the better contaminant removal performance through biogeochemical processes. Therefore, those microbial activities and related indicators could be considered for wetland management such as operation and performance monitoring of wetlands.