• 한국농공학회논문집
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• 제57권2호
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• pp.93-101
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• 2015

The purpose of this study is to evaluate the paddy irrigation efficiency using real-time water level monitoring data and intermittent irrigation model in Gimjae, Dong-Jin irrigation district. For this study, the real-time water level data in Gimjae main canal and other secondary canals were collected from 2012 to 2014 and converted to daily discharge using rating curve in each canal. From intermittent irrigation model in paddy, irrigation water requirement was estimated and irrigation efficiency was calculated. The average amount of irrigation water supply per unit irrigation area was 1,011 mm in Gimjae main canal for 12,749 ha irrigation area, 1,011 mm in the secondary canal of upper region and 1,470 mm in the secondary canal of lower region. The median irrigation loss was 43 % in Gimjae main canal, 25 % in secondary canal of upper region and 35 % in the secondary canal of lower region. The larger irrigation area is, the irrigation loss rates tend to decrease in secondary canals. Monthly median irrigation losses in upper region were 10 (June) - 40 % (September) and those in lower region were 25 (May) to 40 % (April, June, August, and September). The results of canal management loss can be available as the basic data for irrigation water management and estimating guideline of optimal irrigation water supply to improve agricultural water use efficiencies.

• 한국미생물·생명공학회지
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• 제46권2호
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• pp.154-161
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• 2018

현재 도시의 도시열섬현상을 완화시키는 방안으로 도시 녹화사업 및 연구가 주목 받고 있다. 이 연구에서는 질소고정균을 분리하고, 식물 성장에 미치는 영향을 확인했다. 먼저 질소고정 균을 분리하기 위해, 질소원이 없는 배지에서 enrichment를 실시했고, 질소원이 제한된 배지에서 높은 성장을 보인 colony를 분리하여 순수분리 했다. 순수 분리된 균은 ARA를 통해 acetylene이 90% 이상 감소되고, ethylene 생성을 통해 nitrogenase의 활성을 간접적으로 확인했다. 재현성이 확인된 Cedecea sp. MK7과 Enterobacter sp. Y8을 선별했다. 선별된 질소고정 균을 perennial rye grass의 성장에 적용한 결과 건조중량이 18.65 mg인 대조군에 비해 34.80 mg (186.60%)으로 증가한 것을 확인했다. 식물 성장 후, 질소고정 균이 접종된 토양의 미생물 군집 분석은 대조군과 유사했다. 따라서 본 연구에서는 도시녹화 시스템에 질소고정 균을 이용하여 식물 성장을 촉진한다면 그 효율이 증대될 것이다.

• Journal of Microbiology and Biotechnology
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• 제28권11호
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• pp.1823-1833
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• 2018

Fluorescence in-situ hybridization (FISH) is a common and popular method used to investigate microbial communities in natural and engineered environments. In this study, two specific 16S rRNA-targeted oligonucleotide probes, CLZ and KCLZ, were designed and verified to quantify the genus Clostridium and the species Clostridium kluyveri. The optimal concentration of hybridization buffer solution for both probes was 30% (w/v). The specificity of the designed probes was high due to the use of pellets from pure reference strains. Feasibility was tested using samples of Chinese liquor from the famed Luzhou manufacturing cellar. The effectiveness of detecting target cells appears to vary widely in different environments. In pit mud, the detection effectiveness of the target cell by probes CLZ and KCLZ was 49.11% and 32.14%, respectively. Quantitative analysis by FISH technique of microbes in pit mud and fermented grains showed consistency with the results detected by qPCR and PCR-DGGE techniques, which showed that the probes CLZ and KCLZ were suitable to analyze the biomass of Clostridium spp. and C. kluyveri during liquor fermentation. Therefore, this study provides a method for quantitative analysis of Clostridium spp. and C. kluyveri and monitoring their community dynamics in microecosystems.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1772-1781
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• 2007

Activated sludge was sequentially adapted to benzene, toluene, and o-xylene (BTX) to study the effects on the change of microbial community. Sludge adapted to BTX separately degraded each by various rates in the following order; toluene>o-xylene>benzene. Degradation rates were increased after exposure to repeated spikes of substrates. Eleven different kinds of sludge were prepared by the combination of BTX sequential adaptations. Clustering analyses (Jaccard, Dice, Pearson, and cosine product coefficient and dimensional analysis of MDS and PCA for DGGE patterns) revealed that acclimated sludge had different features from nonacclimated sludge and could be grouped together according to their prior treatment. Benzene- and xylene-adapted sludge communities showed similar profiles. The sludge profile was affected from the point of the final adaptation substrate regardless of the adaptation sequence followed. In the sludge adapted to 50 ppm toluene, Nitrosomonas sp. and bacterium were dominant, but these bands were not dominant in benzene and benzene after toluene adaptations. Instead, Flexibacter sp. was dominant in these cultures. Dechloromonas sp. was dominant in the culture adapted to 50 ppm benzene. Thauera sp. was the main band in the sludge adapted to 50 ppm xylene, but became vaguer as the xylene concentration was increased. Rather, Flexibacter sp. dominated in the sludge adapted to 100 ppm xylene, although not in the culture adapted to 250 ppm xylene. Two bacterial species dominated in the sludge adapted to 250 ppm xylene, and they also existed in the sludge adapted to 250 ppm xylene after toluene and benzene.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1185-1192
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• 2012

This study investigated the hydrocarbonoclastic microbial community present on weathered crude oil and their ability to degrade weathered oil in seawater obtained from the Gulf St. Vincent (SA, Australia). Examination of the native seawater communities capable of utilizing hydrocarbon as the sole carbon source identified a maximum recovery of just $6.6{\times}10^1\;CFU/ml$, with these values dramatically increased in the weathered oil, reaching $4.1{\times}10^4\;CFU/ml$. The weathered oil (dominated by > $C_{30}$ fractions; $750,000{\pm}150,000mg/l$) was subject to an 8 week laboratory-based degradation microcosm study. By day 56, the natural inoculums degraded the soluble hydrocarbons (initial concentrations $3,400{\pm}700mg/l$ and $1,700{\pm}340mg/l$ for the control and seawater, respectively) to below detectable levels, and biodegradation of the residual oil reached 62% ($254,000{\pm}40,000mg/l$) and 66% ($285,000{\pm}45,000mg/l$) in the control and seawater sources, respectively. In addition, the residual oil gas chromatogram profiles changed with the presence of short and intermediate hydrocarbon chains. 16S rDNA DGGE sequence analysis revealed species affiliated with the genera Roseobacter, Alteromonas, Yeosuana aromativorans, and Pseudomonas, renowned oil-degrading organisms previously thought to be associated with the environment where the oil contaminated rather than also being present in the contaminating oil. This study highlights the importance of microbiological techniques for isolation and characterisation, coupled with molecular techniques for identification, in understanding the role and function of native oil communities.

• 한국물환경학회지
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• 제23권5호
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• pp.636-641
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• 2007

A pilot-scale biofilm nitiritation reactor was operated with the reject water from a large wastewater treatment plant. The effects of various operating parameters including pH, temperature, dissolved oxygen, solids and organic concentrations were examined. A stable nitritation was achieved at operating pH range of 7.3 to 8.8 with an alkalinity addition. Higher operating temperature of $35{\pm}0.7^{\circ}C$ achieved more stable nitritation compared to $30{\pm}0.2^{\circ}C$. It has been noticed that nitrite accumulation maintained with DO, solids and organic concentrations range of 0.8 to 3.9 mg/L, 3,400 to 11,000 mg/L, and 86 to 572 mg/L, respectively. It seems that the accumulation of nitrite was caused by both the inhibition of $NO_2{^-}$ oxidizers due to free ammonia and the maintenance of the high operating temperature of $35^{\circ}C$ which promote to accumulate the $NH_4{^+}$ oxidizers in the reactor. According to microbial community analysis of fluorescence in situ hybridization and INT-Dehydrogenase measurements, more nitrifiers were presented in attached form compared to suspended growth.

• 상하수도학회지
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• 제22권2호
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• pp.259-265
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• 2008

Generally speaking, there are two widely used methods of Nitrogen removal from waste water: 1) nitrification using autotrophic microorganisms, and 2) denitrification using heterotrophic microorganisms. The C/N ratio is an important factor of the denitrification process. In this case, if methanol is added to increase the lacking organic matter, a high economic cost is incurred and methanol is left in the processed water. In an effort to fix these issues, autotrophic denitrification through the use of Hydrogen, Iron and Sulfur is being studied, and among those Sulfur is cheaper and carries out denitrification effectively, and therefore is being studied the most. In this study, after cultivating T. denitrificans, the presence of T. denitrificans was determined and the effectiveness of denitirification via T. denitrificans was studied. In order to find out about the inhibition of T. denitrificans from the loading of organic matter, this shows that the greater the loading of organic matter, the more the denitrification ability of T. denitrificans is hindered. In order to research the hindrance of T. denitrificans resulting from the loading of $NO_3{^-}-N$, these results show that concentrations less than 100mg/L per 100mL of gel volume do not hinder T. denitrificans. In order to research the optimization of denitrification resulting from T. denitrificans, three 500mL samples of Sulfur granules were prepared: 1) one with only T. denitrificans attached (Mode I), 2) one with both T. denitrificans and active sludge attached (Mode II), and 3) one with only active sludge attached (Mode III). The results showed that autotrophic denitrification using S from Mode I was the most active.

• 한국물환경학회지
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• 제21권6호
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• pp.624-629
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• 2005

The purpose of this study is to investigate the performance of nitrogen removal using autotrophic microorganism in the Membrane-Attached Biofilm Reactor (MABR). The treatment system consists of an aerobic MABR (R1) for nitrification and an anaerobic MABR (R2) for hydrogenotrophic denitrification. Oxygen and hydrogen were supplied through the lumen of hollow-fiber membranes as electron acceptor and donor, respectively. In phase Ι, simultaneous organic carbon removal and nitrification were carried out successfully in R1. In phase II, to develop the biofilm on the hollow-fiber membrane surface and to acclimate the microbial community to autotrophic condition, R1 and R2 were operated independently. The MABRs, R1 and R2 were connected in series continuously in phase III and operated at HRT of 8 hr or 4 hr with $NH_4{^+}-N$ concentration of influent, from 150 to 200 mgN/L. The total nitrogen removal efficiency reached the maximum value of 99% at the volumetric nitrogen loading rate of $1.20kgN/m^3{\cdot}d$ in the combined MABR system with R1 and R2. The results in this study demonstrated that the combined MABR system could operate effectively for the removal of nitrogen in wastewater not containing organic materials and can be used stably as a high rate nitrogen removal technology.

• 한국식품위생안전성학회지
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• 제11권4호
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• pp.315-321
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• 1996

Various antimicrobial drug screen tests have been used in order to ensure food safety. However, the conventional screen tests, the Swab Test on Premises(STOP, USA), the Calf Antibiotic and Sulfa Test(CAST, USA) and the European Economic Community 4-plate Test(FPT, EU) are not sufficiently rapid or sensitive enough to detect low levels of sulfa drugs in meat. We developed a new screen test kit for the determination of the antimicrobial residues in meat called the Bacillus megaterium Disk Assay(BmDA). A comparison of BmDA with the older screen tests showed BmDA was as good as the older ones with several advantages. The new test kit is faster-it can be read in 4∼6 hours instead of 16∼18 hours. Moreover, BmDA can discriminate sulfa drugs from other antimicrobial drugs because p-aminobenzoic acid countacts the inhibiting action of sulfa drugs. Minimum detectable levels of sulfa drugs were significantly improved at the lever of 0.025*0.1 pp, compared with the level of 1.0 ppm in FPT. A comparison of BmDA with the older screen tests in HPLC confirmed meat samples exceeded the Korean tolerance value of 0.1 ppm showed BmDA was the most sensitive in the microbiological screen tests. As the microbiological screen tests have already known, a person familiar with simple laboratory techniques should have no difficulty in using it to detect antimicrobial residues in meat. This would be a simple, economic method of antimicrobial residues detection which might be succesfully used by many laboratories.

• Asian-Australasian Journal of Animal Sciences
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• 제28권4호
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• pp.584-591
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• 2015

This study characterized the fecal bacterial community structure and inter-individual variation in 30-week-old Duroc pigs, which are known for their excellent meat quality. Pyrosequencing of the V1-V3 hypervariable regions of the 16S rRNA genes generated 108,254 valid reads and 508 operational taxonomic units at a 95% identity cut-off (genus level). Bacterial diversity and species richness as measured by the Shannon diversity index were significantly greater than those reported previously using denaturation gradient gel electrophoresis; thus, this study provides substantial information related to both known bacteria and the untapped portion of unclassified bacteria in the population. The bacterial composition of Duroc pig fecal samples was investigated at the phylum, class, family, and genus levels. Firmicutes and Bacteroidetes predominated at the phylum level, while Clostridia and Bacteroidia were most abundant at the class level. This study also detected prominent inter-individual variation starting at the family level. Among the core microbiome, which was observed at the genus level, Prevotella was consistently dominant, as well as a bacterial phylotype related to Oscillibacter valericigenes, a valerate producer. This study found high bacterial diversity and compositional variation among individuals of the same breed line, as well as high abundance of unclassified bacterial phylotypes that may have important functions in the growth performance of Duroc pigs.