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http://dx.doi.org/10.4014/mbl.1712.12009

Nitrogen Fixation Screening and Plant Growth Assessment for Urban Greening  

Jeong, Sun Hwan (Department of Life Science, Graduate School, Kyonggi University)
Lee, Sang Seob (Department of Life Science, Graduate School, Kyonggi University)
Publication Information
Microbiology and Biotechnology Letters / v.46, no.2, 2018 , pp. 154-161 More about this Journal
Abstract
Currently, urban greening projects and research are attracting attention as a way to mitigate urban heat island phenomenon. In this study, nitrogen fixative bacteria were isolated and their effects on plant growth were confirmed. First, enrichment was performed in a nitrogen-free medium to isolate the nitrogen-fixing bacteria, and the colony showing high growth in a medium with limited nitrogen source was isolated and purified. Separated bacterial isolates were reduced by more than 90% acetylene by ARA and indirectly confirmed the activity of nitrogenase by ethylene production. Cedecea sp. MK7 and Enterobacter sp. Y8 with confirmed reproducibility were selected as nitrogen fixative bacteria. Nitrogen fixing bacteria were applied to the growth of perennial rye grass, and it was found that the dry weight increased to 34.80 mg (186.60%) compared with the control with 18.65 mg dry weight. After plant growth, microbial community analysis of soil applied by bacteria showed similarity to the control group. Therefore, in this study, it is expected that the efficiency will be increased if plant growth is promoted by using nitrogen fixing bacteria in urban greenery system.
Keywords
Nitrogen fixation; fertilizer; Cedecea sp.; urban greening;
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