• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2002.09a
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• pp.310-314
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• 2002

To investigate the effect of on-site bioremediation in soil that have been contaminated by hydrocarbon fuel spills, petroleum-degrading bacteria isolated from soil around petroleum chemical industry and microbial agents were constructed. We investigated biopiles for on-site bioremediation of soil contaminated (5000 mg per kg) with bunker A fuel in five independent lab-scale experiments. Five biopile units constituting the following treatments: (1) control with no nutrients and microbial agents (2) microbial agent M plus nutrients (3) microbial agent C plus nutrients (4) only microbial agent C (5) control with only nutrients. The results were highly different one another. After 30 days in treatments with optimal condition, total petroleum hydrocarbons were reduced to below 10 mg per kg of soil at the biopile units mixed with microbial agents, but control biopile units show that were reduced from 1,105 to 2,588 mg per kg of soil. Our results show that microbial agents at on-site bioremediation of fuel-contaminated soil is highly effective.

• Microbiology and Biotechnology Letters
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• v.40 no.1
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• pp.30-38
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• 2012

We investigated the effects on soil microbial diversity and the growth promotion of red pepper resulting from inoculation with a microbial agent composed of Bacillus subtilis AH18, B. licheniformis K11 and Pseudomonas fluorescens 2112 in a red pepper farming field. Photosynthetic bacteria, Trichoderma spp., Azotobacter spp., Actinomycetes, nitrate oxidizing bacteria, nitrite oxidizing bacteria, nitrogen fixing bacteria, denitrifying bacteria, phosphate solubilizing bacteria, cellulase producing bacteria, and urease producing bacteria are all indicator microbes of healthy soil microbial diversity. The microbial diversity of the consortium microbial agent treated soil was seen to be 1.1 to 14 times greater than soils where other commercial agent treatments were used, the latter being the commercial agent AC-1, and chemical fertilizer. The yield of red pepper in the field with the treated consortium microbial agent was increased by more than 15% when compared to the other treatments. Overall, the microbial diversity of the red pepper farming field soil was improved by the consortium microbial agent, and the promotion of growth and subsequent yield of red pepper was higher than soils where the other treatments were utilized.

• Journal of Environmental Science International
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• v.24 no.12
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• pp.1705-1710
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• 2015

The aim of the present study was to determine the effects of soil microbial agent with red ginseng marc on growth of watermelon during 5 months. The three treatments were distributed in a completely randomized design with four replicates per plot. After 1 week in planting dates, the growth of watermelon (full length, stem thichness, leaf length and lead width) showed no significant difference in all treatments. During elongation stage (20 days), soil microbial agent with red ginseng marc was increased by 5% in leaf thickness (May 23) and 7~14% in leaf length (May 16 and 23) when compared to other treatments. For changes in fruit bearing thickness, there were no differences among treatments. Characteristics of watermelon in harvest season have an effect on harvest and length, stalk length, naval length, weight, sugar content and yield, except for harvest and width. In particular, yields increased with treatments with two soil microbial agent (7~12%), indicating that soil microbial agent with red ginseng marc showed higher yield than the other treatments. In conclusion, red ginseng marc-treated soil microbial agents have a positive effect on the harvest season of watermelon and can provide useful information for the selection of the functional microbial properties and the registration of microbial fertilizer.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2004.04a
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• pp.39-42
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• 2004

Landfarming and a microbial agent, Bioil-D, were used to treat diesel-contaminated soil. The microbial agent was applied to the contaminated soil in a concentration of 5$\times$10$^2$ cfu/soil(g) and the total amount of microbial agent, 210$\ell$, was sprinkled on the soil four times for 24 days during 50 day-remediation period. The remediation goal, lower than 800mg/kg of TPH, was achieved from 5, 707 mg/kg of TPH within 50 days. The total number and activity of indigenous microorganisms were increased by 100 times after the microbial agent, Bioil-D, was applied to the contaminated soil.

• Bulletin of Korea Environmental Preservation Association
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• v.26 no.5_6
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• pp.59-61
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• 2004

Landfarming and a microbial agent were used to treat dilsel-contaminated soil. The microbial agent was applied to the contaminated soil in a concentration of 5$\times$102 cfu/soil(g) and the total amount of microbial agent, 210 l , was sprinkled on the soil four times for 24 days during 50 day-remediation period. The remediation goal, lower than 800mg/kg of TPH, was achieved from 5,707mg/kg of TPH within 50 days. The total number and activity of indigenous microorganisms were increased by 100 times after the microbial agent, Bioil-D, was applied to the contaminated soil.

• Journal of Environmental Science International
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• v.21 no.10
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• pp.1187-1193
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• 2012

The aim of this research is to enhance the bottom environment of Geoje fish farm that has been severely contaminated. Treatment of microbial agent and/or calcium oxide significantly changed that environment: in ignition loss, either treatment (25% or 21%) showed better than mixed treatment (13.2%). In COD, the oxygen releasing agent or mixed treatment reduced the index by more than 20%. In T-P and T-N, the effects of $CaO_2$ on them were overwhelming (50% or more) meanwhile that of the microbial agent on them was less than 20%. Also, $CaO_2$ influenced on the microbial flora: Desulfobvibrio thermophilus, a sulfate reducing bacterium decreased in number, considering the increase of pH and rise of redox potential. In contrast, Pseudomonas sp., Pseudoalteromonas sp., Pseudomonas aeruginosa were remarkably dominant over other species with mixed treatment as a PCA analysis confirmed it.

• Environmental Engineering Research
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• v.21 no.1
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• pp.52-57
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• 2016

Sediment is a useful natural source but deteriorated continually by anthropogenic and industrial sources. Therefore, it is imperative to search a suitable method for improving or restoring sediment quality. Sediment has been tested to identify the effects of some external agents on a polluted area for 28 days. Chemical analysis and total viable counts (TVC) test have been conducted for 4 days interval to assess their performance. The analyses of chemical oxygen demand (COD), acid volatile sulfide (AVS), total phosphorous (T-P), total nitrogen (T-N) indicate that the chemical agents was more efficient to improve sediment quality whereas the microbial agent was more efficient for nutrient releasing from sediment. Oxygen releasing property of the chemical agent was thought to be providing with more congenial environment for the higher growth of the bacterial community than the direct application of microbial agents.

• Journal of the Korea Organic Resources Recycling Association
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• v.11 no.3
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• pp.75-86
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• 2003

In the near future, the capacity of conventional anaerobic digester is thought to be insufficient because of the increase of the total solids from expansion of intercepting sewer, sewage quantity and direct input of night soil from near apartment districts. The objectives of this study was to investigate the improvement of digestion efficiency using microbial agent(Bio-dh). The system was a pilot-scale, two-staged, anaerobic sludge digestion system. The first-stage digester was heated and mixed. The agitation velocity of the first-stage digester was 120rpm. The second-stage digester was neither heated nor mixed. The Digestion temperature was kept at $35{\pm}1^{\circ}C$ The detention time of digester was 19 days. The dosage of sewage sludge and microbial agent were $0.65m^3/day$ and $0.5{\ell}/day$, respectively. The experiments was run for 25days. Three times a week, $COD_{Mn}$ and SS of effluent, TS, VS, and biogas production rate were measured. Temperature, pH, and alkalinity were measured daily. The results were as follows ; Without microbial agent, digestion efficiencies ranged 46.0%~50.9%(mean=48.6%), with microbial agent(Bio-dh), digestion efficiencies ranged 52.8%~57.3%(mean=54.2%). Consequently, microbial agent(Bio-dh) increased the sludge digestion efficiency about 12%. Also, Without microbial agent, the mean concentration of $COD_{Mn}$ and SS of second-stage digester effluent were 1,639mg/L, 4,888mg/L respectively. With microbial agent, the mean concentration of $COD_{Mn}$ and SS of second-stage digester effluent were 859mg/L, 2,405mg/L respectively. Consequently, microbial agent(Bio-dh) increased the removal efficiency of $COD_{Mn}$ and SS about 47.6% and 50.8%, respectively.

• Fashion & Textile Research Journal
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• v.7 no.1
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• pp.81-84
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• 2005

Skin patch test and antibacterial properties of the reaction products between poly(hexamethyl biguanide) hydrochloride and trimethylol melamine on textile fabrics were examined. Antibacterial activities of anti-microbial agent treated samples are very good. The reduction ratios against four kinds of colonies are 99.9 % after repeated laundering ten times. Skin patch test results for anti-microbial agent treated samples are almost-negative by Hi-scope judgement and macroscopical judgement.

• The Journal of the Convergence on Culture Technology
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• v.4 no.2
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• pp.179-183
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• 2018

Recently, several studies have been conducted on biogas and organic compost production using food waste in an anaerobic digester. In this study, basic experiments were conducted to produce biogas and compost by fermenting food wastes with microbial agents. First, a microbial agent was developed by combining various microorganisms. Then, the amount of generated biogas was identified through a food waste batch experiment. Further, we could maximize and demonstrate biogas production in an anaerobic digester by examining biogas production and composting in a pilot plant.