• 한국응용과학기술학회지
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• 제14권2호
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• pp.93-102
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• 1997

Isothiazoline derivatives is widely used to food, medical drug and industrial goods, cosmetics etcs, and it makes to restrain and to sterilize a breeding of microbe as a preservative and a sterilizing agent. It differs with the raw material of paraoxybenzoic acid derivatives or imidazolydinyl urea to be in use at present, on the efficacy and effect, and has various characteristics. This synthesis makes 3,3'-dithiodipropionic chloride to add a thionyl chloride in 3,3'-dithiodipropionic acid, and 3,3'-dithiodipropionic methyl amide makes to synthesize in a reflux reaction the mono methyl amine to 3,3'-dithiodipropionic chloride. And last synthesis becomes to make chlorination-cyclization molecule doing a reflux reaction in the temperature of $90{\sim}100^{\circ}C$ to mix excessively thionyl chloride and ethylene dichloride to 3,3'-dithiodipropionic methyl amide. The last synthesis material has got in the mixture of 5-chloro-2-methyl-4-isothiazoline-3-one and 2-methyl-4-isothiazoline-3-one, and it is so-called isothiazoline derivatives. The purification of isothiazoline derivatives makes to fuse in ethyl acetate, and makes to decolorize and to deodorize in recrystallization. This experiment has been in synthesis and purification of isothiazoline derivatives, and has tried to measure on the antisepsis and sterilization function of microbe according to pH or content change.

• Journal of Microbiology and Biotechnology
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• 제14권1호
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• pp.212-215
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• 2004

In order to develop a biocontrol agent that can effectively control Fusarium wilt on Cymbidium genus, the effectiveness of antagonistic microbes against the cause pathogen was screened. The selected microbe showed a broad spectrum of antifungal activity, and the culture broth of this microbe had better preventive effect on Fusarium wilt than the commercial chemical agent in the pot assay. This isolated strain, GBA-12, was identified as Streptomyces kasugaensis, and the antifungal substance was purified from a broth culture of GBA-12. This purified substance was identified as a polyene macrolide (YS-822A) that was newly discovered from Streptomyces kasugaensis, and it exhibited antifungal activity against several phytopathogenic fungi.

• The Plant Pathology Journal
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• 제36권2호
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• pp.185-191
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• 2020

Streptomyces griseus S4-7, a well-characterized keystone taxon among strawberry microbial communities, shows exceptional disease-preventing ability. The whole-genome sequence, functional genes, and bioactive secondary metabolites of the strain have been described in previous studies. However, proteomics studies of not only the S4-7 strain, but also the Streptomyces genus as a whole, remain limited to date. Therefore, in the present study, we created a proteomics reference map for S. griseus S4-7. Additionally, analysis of differentially expressed proteins was performed against a wblE2 mutant, which was deficient in spore chain development and did not express an antifungal activity-regulatory transcription factor. We believe that our data provide a foundation for further in-depth studies of functional keystone taxa of the phytobiome and elucidation of the mechanisms underlying plant-microbe interactions, especially those involving the Streptomyces genus.

• 멤브레인
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• 제20권3호
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• pp.173-184
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• 2010

미생물 연료전지는 신재생에너지로서 미생물이 유기물을 분해하는 신진대사 과정을 통해서 전기에너지를 생성한다. 각종 유기물이 풍부한 폐수를 이용하여 전력을 생산할 뿐 아니라, 슬러지 발생량도 감축할 수 있는 미래 전도유망한 친환경에너지이다. 하지만 이를 상용화하기 위해서는 전지 내부에서 발생하는 모든 저항요소들을 감소시켜 더 높은 전력밀도를 생산해야 될 필요가 있다. 예를 들어 신진대사가 활발한 미생물의 종류, 미생물과 전극의 효과적인 전자전달 과정, 전극의 재료 및 형태 등의 개선을 통하여 전력밀도를 높일 수 있다. 특히, 고분자 전해질 분리막의 성능개선은 산화, 환원전극조를 완벽히 분리할 뿐만 아니라, 환원전극으로의 수소이온 전도도를 높여 내부저항을 줄일 수 있는 핵심 요소이다.

• 한국식품저장유통학회지
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• 제6권3호
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• pp.339-344
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• 1999

김치의 향미개선 및 게껍질 분말 첨가김치의 부미를 제거하기 위한 향미개선제로서 우골추출물의 첨가효과를 검토하였다 우골추출물은 김치의 숙성중 pH와 산도, 총균수, 젖산균비, Leuconostoc과 Lactobacilli의 수에는 큰 영향을 미치지 못하였으나 산미와 김치냄새를 감소시키고 종합적 맛을 개선시키는 향미 개선제로서의 역할을 하였다. 또 우골추출물(0.03%)을 게껍질분말(1.5%)과 함께 첨가할 경우는 게껍질분말만을 첨가한 경우보다 높은 pH를 유지하는 한편 완충효과가 증가되었으며 젖산균비가 높아졌다. 관능검사에 의하여 평가한 결과 게껄질분말을 단독으로 첨가한 경우보다 우골추출물을 병용하여 첨가한 김치에서 비린 맛이 낮아졌으며, 아삭아삭한 맛과 종합적인 맛은 숙성전반에 걸쳐 높게 나타났다.

• Molecules and Cells
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• 제25권3호
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• pp.323-331
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• 2008

Plants are continually exposed to a variety of potentially pathogenic microbes, and the interactions between plants and pathogenic invaders determine the outcome, disease or disease resistance. To defend themselves, plants have developed a sophisticated immune system. Unlike animals, however, they do not have specialized immune cells and, thus all plant cells appear to have the innate ability to recognize pathogens and turn on an appropriate defense response. Using genetic, genomic and biochemical methods, tremendous advances have been made in understanding how plants recognize pathogens and mount effective defenses. The primary immune response is induced by microbe-associated molecular patterns (MAMPs). MAMP receptors recognize the presence of probable pathogens and evoke defense. In the co-evolution of plant-microbe interactions, pathogens gained the ability to make and deliver effector proteins to suppress MAMP-induced defense responses. In response to effector proteins, plants acquired R-proteins to directly or indirectly monitor the presence of effector proteins and activate an effective defense response. In this review we will describe and discuss the plant immune responses induced by two types of elicitors, PAMPs and effector proteins.

• 응용통계연구
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• 제26권6호
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• pp.923-932
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• 2013

이 논문에서는 우리나라에서 발생하는 원인균별 식중독 발생건수를 예측하는 방법을 제안한다. 우리나라에서 보고되는 주별 식중독 발생 건수를 원인균로 나누면 자료에 많은 0의 관측값이 포함되어 있으며 식중독 발생 간에 종속성을 가진다. 이 현상을 모형화하기 위해 이 논문에서는 전체 식중독 건수를 자기회귀모형으로 예측하고 원인균별 식중독 발생 확률을 다범주 로짓모형으로 추정한다. 예측된 식중독 건수와 추정된 원인균별 식중독 발생 확률을 곱하여 원인균별 식중독 발생건수를 예측한다. 제안된 방법의 타당성을 확인하기 위해 평균제곱오차와 평균절대편차를 이용하여 제안 방법과 영과잉모형을 비교해 본다.

• Journal of Microbiology and Biotechnology
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• 제21권11호
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• pp.1184-1192
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• 2011

A bacterial isolate (strain JS-2) characterized as Bacillus sp. was challenged with high concentrations of toxic selenite ions. The microbe was found to transform the toxic, soluble, colorless selenite (${SeO_3}^{2-}$) oxyions to nontoxic, insoluble, red elemental selenium ($Se^0$). This process of biotransformation was accompanied by cytoplasmic and surface accumulation of electron dense selenium ($Se^0$) granules, as revealed in electron micrographs. The cells grown in the presence of selenite oxyions secreted large quantities of extracellular polymeric substances (EPS). There were quantitative and qualitative differences in the cell wall fatty acids of the culture grown in the presence of selenite ions. The relative percentage of total saturated fatty acid and cyclic fatty acid increased significantly, whereas the amount of total unsaturated fatty acids decreased when the cells were exposed to selenite stress. All these physiological adaptive responses evidently indicate a potentially important role of cell wall fatty acids and extracellular polymeric substances in determining bacterial adaptation towards selenite-induced toxicity, which thereby explains the remarkable competitiveness and ability of this microbe to survive the environmental stress.

• 한국재난정보학회 논문집
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• 제5권1호
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• pp.10-27
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• 2009

The fear briefly became reality in the U.S. after 9/11, as man-made anthrax spores were found in letters and in east coast post offices on a number of occasions. Anthrax attacks must be related to the terrorist attacks of September 11. These two accidents changed the situation completely. Characteristics are summarized as belows. First, it is necessary for all countries to create each own appropriate countermeasures against bioterrorism. In addition, it is urgent to consider countermeasures focusing on bioterror characteristics that terrorists may use biological agents because they can be extremely difficult to detect and do not cause illness for several hours to several days. Otherwise, those attacks will also show the panic and very real danger posed by the release of such toxins on an unsuspecting public. Second, it is crucial to manage the result thoroughly. A biological weapon is useful to a terrorist group mainly as a method of creating mass panic and disruption to a society. Because it is not easy to predict and collect information about how terrorists utilize pathogenic microbe as well as terror measures in their activities, more realistic approach should be taken to reduce damage. Third, to expand investment in research and development is necessary. Considering advances in microbiology and genetic engineering lately, it is likely that new pathogenic microbe would be created by the enemy. For that matter, it is imperative that medical countermeasures should be devised.

• 한국유기농업학회지
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• 제19권spc호
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• pp.149-152
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• 2011

To improve the soil condition for no-tillage organic pepper cultivation, four different green manure crops were cultivated. Fertilizer supply was depended on the biomass of the cultivated green manure crops, nitrogen supplies were 314kg in Vicia villosa and 341kg $ha^{-1}$ in Vicia angustifolia. In the microbial community analyzed by phospholipid fatty acid (PLFA) method, soil microbe populations were different among the green manure crops and fungi group was increased at Vicia angustifloia and Vicia villosa. The biological ratio indexes of fatty acids in the soils, the ratio of Gram-negative to Gram-positive bacterial PLFA and Ratio of aerobes to anaerobes were high at Vicia hirsute and Vicia tetrasperma suggesting the enrich of the aerobic conditions. The ratio of saturated to unsaturated fatty acids increased at Vicia angustifloia and Vicia villosa suggesting anaerobic conditions. Abundant biomass and uncomposted organic matter, the ratio of fungi to bacteria was increased at Vicia angustifloia and Vicia villosa.