• 제목/요약/키워드: Micro-propagation

검색결과 240건 처리시간 0.025초

Silver nitrate and silver-thiosulphate mitigates callus and leaf abscission during Shisham clonal micro-propagation

  • Raturi, Manoj Kumar;Thakur, Ajay
    • Journal of Plant Biotechnology
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    • 제48권3호
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    • pp.173-178
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    • 2021
  • Basal callus formation and leaf abscission is a problem in clonal micropropagation. We have described an in vitro clonal propagation protocol of Dalbergia sissoo Roxb (shisham) 'FRI-14' in which AgNO3 played important role not only in mitigating problem of leaf abscission and basal callus, but also improved shoot induction and multiplication. Best induction and shoot multiplication was obtained on MS media with 1.5 mg/l 6-BAP and 10 mg/l AgNO3 and half-strength MS media with 0.5 mg/l 6-BAP, 2 mg/l AgNO3 and 50 mg/l Adenine sulphate whereas best ex vitro rooting was obtained with 200 mg/l IBA in pulse treatment.

Identification and Three-Dimensional Characterization of Micropore Networks Developed in Granite using Micro-Focus X-ray CT

  • Choo, Chang-Oh;Takahashi, Manabu;Jeong, Gyo-Cheol
    • 지질공학
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    • 제24권2호
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    • pp.179-189
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    • 2014
  • We analyzed the three-dimensional distribution of micropores and internal structures in both fresh and weathered granite using micro-focus X-ray computed tomography (micro-CT). Results show that the pore radius in fresh granite is mostly in the range of $17-50{\mu}m$, the throat radius is in the range of $5-25{\mu}m$, and the coordination number (CN) of pores is less than 10. In contrast, the pore radius in weathered granite is mostly in the range of $20-80{\mu}m$, the throat radius is in the range of $8-30{\mu}m$, and the CN is less than 12. In general, a positive linear relationship exists between pore radius and CN. In addition, both the size and the density of pores increase with an increasing degree of rock weathering. The size of the throats that connect the pores also increases with an increasing degree of weathering, which induces fracture propagation in rocks. Micro-CT is a powerful and versatile approach for investigating the three-dimensional distributions of pores and fracture structures in rocks, and for quantitatively assessing the degree of pore connectivity.

Experimental Determination of Concrete Fracture Properties with Modified S-FPZ Model

  • Yon, Jung-Heum;Kim, Tai-Hoon
    • International Journal of Concrete Structures and Materials
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    • 제18권3E호
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    • pp.213-219
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    • 2006
  • Modified singular fracture process zone(S-FPZ) model is proposed in this paper to determine a fracture criterion for continuous crack propagation in concrete. The investigated fracture properties of the proposed fracture model are strain energy release rate at a micro-crack tip and the relationship between crack closure stress(CCS) and crack opening displacement(COD) in the FPZ. The proposed model can simulate the actual fracture energy of experimental results fairly well. The results of the experimental data analysis show that specimen geometry and loading condition did not affect the CCS-COD relation. However, the strain energy release rate is a function of not only specimen geometry but also crack extension. The strain energy release rate remained constantly at the minimum value up to the crack extension of 25 mm, and then it increased linearly to the maximum value. The maximum fracture criterion occurred at the peak load for specimens of large size. The fracture criterion remained at the maximum value after the peak load. The variation of the fracture criterion is caused by micro-cracking and micro-crack localization. The fracture criterion of strain energy release rate can simply be the size effect of concrete fracture, and it can be used to quantify the micro-cracking and micro-crack localizing behavior of concrete.

A Study on the Crevice Corrosion for Ferritic Stainless Steel by Micro Capillary Tube Method

  • Na Eun-Young;Ko Jae-Yong;Baik Shin-Young
    • 전기화학회지
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    • 제7권4호
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    • pp.179-182
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    • 2004
  • The aim of this study is to investigate the initiation and propagation of crevice corrosion for ferritic stainless steel in artificial crevice based on micro capillary tube method. The 430 stainless steel in artificial crevice is potentiostatically polarized in different sodium chloride solutions. Potentiodynamic and potentiostatic polarization data were measured in situ. The potentials in the crevice were measured by depth profile using the 0.04 mm diameter micro capillary tube inserted in the crevice. The potentials in the crevice ranged from -220 mV to -360 mV vs SCE from opening to bottom of crevice, which are lower than the external surface potential, -200 mV vs SCE. Such a potential drop induced the change of the metal surface state from passive to active. The surface of metal is located in passive state in -200 mV but the inner surface keeps active state below -220 mV, Thus these results show that the It drop mechanism in the crevice was more objective for evaluation and the method was easier to reproduce. Therefore the potential drop is one of the reasons for crevice corrosion by measuring the potentials in narrow crevice with a new micro measuring system.

引張 軸荷重 疲勞 에 의한 微小表面 균열 의 發생 . 成長擧動 (Initiation and Propagation Behaviors of Micro-Surface-Fatigue Cracks under In-Plane Tension Fatigue Tests)

  • 서창민
    • 대한기계학회논문집
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    • 제9권1호
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    • pp.1-9
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    • 1985
  • 본 연구에서는 이러한 점에도 역점을 두어서 초기 피로균열의 발생.거동을 관 찰하기 쉬운 연강을 선택하여 편진인장반복하중을 작용시켜, 연강 평골재의 표면상에 발생.성장하는 미소표면균열의 거동을 전자현미경과 광학현미경을 병용하여 표면과 파 단면을 입체적으로 조사하여, 피로균열의 초기발생상태를 조사하고, 그 성장율을 조사 하여 파괴역학적으로 해석하여 그 특성을 조사한다.

Development of non-destructive testing method to evaluate the bond quality of reinforced concrete beam

  • Saleem, Muhammad;Almakhayitah, Abdulmalik Mohammed
    • Structural Engineering and Mechanics
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    • 제74권3호
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    • pp.313-323
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    • 2020
  • Non-destructive tests are commonly used in construction industry to access the quality and strength of concrete. However, till date there is no non-destructive testing method that can be adopted to evaluate the bond condition of reinforced concrete beams. In this regard, the presented research work details the use of ultra-sonic pulse velocity test method to evaluate the bond condition of reinforced concrete beam. A detailed experimental research was conducted by testing four identical reinforced concrete beam samples. The samples were loaded in equal increments till failure and ultra-sonic pulse velocity readings were recorded along the length of the beam element. It was observed from experimentation that as the cracks developed in the sample, the ultra-sonic wave velocity reduced for the same path length. This reduction in wave velocity was used to identify the initiation, development and propagation of internal micro-cracks along the length of reinforcement. Using the developed experimental methodology, researchers were able to identify weak spots in bond along the length of the specimen. The proposed method can be adopted by engineers to access the quality of bond for steel reinforcement in beam members. This allows engineers to carryout localized repairs thereby resulting in reduction of time, cost and labor needed for strengthening. Furthermore, the methodology to apply the proposed technique in real-world along with various challenges associated with its application have also been highlighted.

Discrete element modeling of strip footing on geogrid-reinforced soil

  • Sarfarazi, Vahab;Tabaroei, Abdollah;Asgari, Kaveh
    • Geomechanics and Engineering
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    • 제29권4호
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    • pp.435-449
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    • 2022
  • In this paper, unreinforced and geogrid-reinforced soil foundations were modeled by discrete element method and this performed under surface strip footing loads. The effects of horizontal position of geogrid, vertical position, thickness, number, confining pressure have been investigated on the footing settlement and propagation of tensile force along the geogrids. Also, interaction between rectangular tunnel and strip footing with and without presence of geogrid layer has been analyzed. Experimental results of the literature were used to validation of relationships between the numerically achieved footing pressure-settlement for foundations of reinforced and unreinforced soil. Models and micro input parameters which used in the numerical modelling of reinforced and unreinforced soil tunnel were similar to parameters which were used in soil foundations. Model dimension was 1000 mm* 600 mm. Normal and shear stiffness of soils were 5*105 and 2.5 *105 N/m, respectively. Normal and shear stiffness of geogrid were 1*109 and 1*109 N/m, respectively. Loading rate was 0.001 mm/sec. Micro input parameters used in numerical simulation gain by try and error. In addition of the quantitative tensile force propagation along the geogrids, the footing settlements were visualized. Due to collaboration of three layers of geogrid reinforcements the bearing capacity of the reinforced soil tunnel was greatly improved. In such practical reinforced soil formations, the qualitative displacement propagations of soil particles in the soil tunnel and the quantitative vertical displacement propagations along the soil layers/geogrids represented the geogrid reinforcing impacts too.

Fe-Ni 합금 클래드 리드 프레임을 이용한 전자 재료 접합부의 품질향상과 그 신뢰성 (Quality improvement on joints of electronic materials and its reliability by Fe-Ni alloy clad lead frame)

  • 신영의;최인수;안승호
    • Journal of Welding and Joining
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    • 제13권2호
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    • pp.82-95
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    • 1995
  • This paper discusses distribution of thermal stress, strain at near the joint and investigates the reliability of solder joints of electronic devices on a printed circuit board. As Electronic devices are composed of different materials, thermal stresses generate at near the interface, such as solder joints and interface between lC device and lead frame pad due to the differences of thermal expansion coefficients, As results of thermal stress, strain, micro crack often occurs thermal fatigue fracture at the interface of different materials, The initiation and propagation of micro crack depend on the environmental conditions, such as storage temperature and thermal cycling. Finally, this paper experimentally shows a way to suppress micro cracks by using Fe-Ni alloy clad lead frame, and investigates crack and thermal fatigue fracture of TSOP(Thin small outline package) type on printed circuit board.

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63Sn-37Pb 땜납의 크리프 특성에 관한 연구 (A Study on the Creep Characteristics of Solder of 63 Sn-37Pb)

  • 이억섭;김의상
    • 한국정밀공학회지
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    • 제21권2호
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    • pp.138-144
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    • 2004
  • The initiation and the propagation of solder joint crack depend on its environmental conditions, such as high temperature creep and thermal fatigue. Creep is known to be the most important factor for the mechanical failure of solder joints in micro-electronic components and micro-systems. This is mainly caused by the different thermal expansion coefficients of the materials used in the micro-electronic packages. To determine the reliability of solder joints and consequently the electronic components, the characterization of the creep behavior of this group of materials is crucial. This paper is to apply the theory of creep into solder joints and to provide related technical information needed for evaluation of reliability of solder joint to failure. 63Sn-37Pb solder was used in this study. This paper experimentally shows a way to enhance the reliability of solder joints.