Browse > Article
http://dx.doi.org/10.5010/JPB.2021.48.3.173

Silver nitrate and silver-thiosulphate mitigates callus and leaf abscission during Shisham clonal micro-propagation  

Raturi, Manoj Kumar (Genetics and Tree Improvement Forest Research Institute)
Thakur, Ajay (Genetics and Tree Improvement Forest Research Institute)
Publication Information
Journal of Plant Biotechnology / v.48, no.3, 2021 , pp. 173-178 More about this Journal
Abstract
Basal callus formation and leaf abscission is a problem in clonal micropropagation. We have described an in vitro clonal propagation protocol of Dalbergia sissoo Roxb (shisham) 'FRI-14' in which AgNO3 played important role not only in mitigating problem of leaf abscission and basal callus, but also improved shoot induction and multiplication. Best induction and shoot multiplication was obtained on MS media with 1.5 mg/l 6-BAP and 10 mg/l AgNO3 and half-strength MS media with 0.5 mg/l 6-BAP, 2 mg/l AgNO3 and 50 mg/l Adenine sulphate whereas best ex vitro rooting was obtained with 200 mg/l IBA in pulse treatment.
Keywords
Clonal propagation; Ethylene inhibitors; Tissue-culture; Tahli; Shisham; Callus;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Tewari D.N. A Monograph on Dalbergia sissoo Roxb; International Book Distributors: Dehra Dun, India, 1994
2 Thirunavoukkarasu M, Panda PK, Nayak P, Behera PR, Satpathy GB (2010) Effect of media type and explant source on micropropagation of Dalbergia sissoo Roxb. - an important multipurpose forest tree. Int Res J Plant Sci 1155-1162
3 Yan H, Liang C, Yang L, Li Y (2010) In vitro and Ex vitro rooting of Siratia grosvenori a traditional medicine plant. Acta Physiol Plant 32:115-120   DOI
4 Drisya Ravi, R. S., Siril, E. A., & Nair, B. R. (2019). The effect of silver nitrate on micropropagation of Moringa oleifera Lam. an important vegetable crop of tropics with substantial nutritional value. Physiology and Molecular Biology of Plants, 25(5), 1311-1322. https://doi.org/10.1007/s12298-019-00689-x   DOI
5 Eeswara JP, Stuchbury T, Allan EJ, Mordue AJ (1998) A standard procedure for the micropropagation of the Neem tree (Azadirachta indica A. Juss). Plant Cell Rep 17:215-219   DOI
6 Hassanein AM, Salem JM, Faheed FA, El-Nagish A (2018) Effect of anti-ethylene compounds on isoenzyme patterns and genome stability during long term culture of Moringa oleifera. Plant Cell Tissue Organ Cult 132:201-212. https://doi.org/10.1007/s11240-017-13260   DOI
7 Joshi I, Bisht P, Sharma VK, Uniyal DP (2003) Studies on effect of nutrient media for clonal propagation of superior phenotypes of Dalbergia sissoo Roxb. through tissue culture. Silvae Genet 52:143-147
8 Kao CH, Yang SF (1983) Role of ethylene in the senescence of detached rice leaves. Plant Physiol 73:881-885   DOI
9 Lemos EEP, Jennet B (2015) Control of leaf abscission in nodal cultures of Annona squamosa L. J Hortic Sci Biotechnol 71:721-728   DOI
10 Majeed F. A, Munir H, Rashid R, & Zub air M. T. (2019). Antimicrobial, cytotoxicity, mutagenicity and anti-epileptic potential of ethanol extracts of a multipurpose medicinal plant Dalbergia sissoo. Biocatalysis and Agricultural Biotechnology, 19, 101155. https://doi.org/10.1016/j.bcab.2019.101155   DOI
11 Mirzai F, Uliaie ED, Hagh AB (2015) Stimulation effect of AgNO3 and CoCl2 as ethylene inhibitors on in vitro organogenesis of sunflower (Helianthus annuus L.). J Agric Sci 25:113-118   DOI
12 Mookkan M, Andy G (2014) AgNO3 boosted high-frequency shoot regeneration in Vigna mungo (L.) Hepper. Plant Signal Behav 9(10):1-5. https://doi.org/10.4161/psb.32165   DOI
13 Murashige T. and Skoog F (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Plant Physiology, 15:473-497   DOI
14 Orwa C, Mutua A, Kindt R, Jamnadass R, Anthony S (2009) Agroforestree Database: A tree reference and selection guide version 4.0.. Available online: http://www.worldagroforestry.org (accessed on 7 December 2018)
15 Pua EC, Chi GL (1993) De novo shoot morphogenesis and plant growth of mustard (Brassica juncea) in vitro in relation to ethylene. Physiol Plant 88:467-474   DOI
16 Riyathong T, Dheeranupattana S, Palee J, Shank L (2010) Shoot multiplication and plant regeneration from in vitro cultures of drumstick tree (Moringa oleifera Lam.). In: The 8th international symposium on biocontrol and biotechnology. King Mongkut's Institute of Technology Ladkrabang and Khon Kaen University, Nongkhai Campus, Thailand, pp 99-104
17 Singh AK, Chand S (2003) Somatic embryogenesis and efficient plant regeneration from cotyledon explants of a timber yielding legumi nous tree Dalbergia sissoo Roxb . J Plant Physiol 160:415-421   DOI
18 Bairu MW, Aremu AO, Staden JV (2011) Somaclonal variation in plants: causes and detection methods. Plant Growth Regul 63:147-173   DOI
19 Beyer EM (1976b) Silver ion: a potent anti-ethylene agent in cucumber and tomato. HortScience 11:175-196
20 Chand S, Singh AK (2005) Plant regeneration from semi-mature zygotic embryos of Dalbergia sissoo Roxb. Indian J Biotech 4:78-81
21 Das P, Samantaray S, Roberts AV, Rout GR (1997) In vitro somatic embryogenesis of Dalbergia sissoo Roxb.-a multipurpose timber yielding tree. Plant Cell Rep 16:578-582   DOI
22 Phulwaria M, Ram K, Harish, Gupta AK, Shekhawat NS (2012b) Micropropagation of mature Terminalia catappa (Indian almond)- a medicinally important forest tree. J Forest Res 17:202-207   DOI
23 Sarropoulou V, Dimassi-Theriou K, Therios I (2016) Effect of the ethylene inhibitors silver nitrate, silver sulfate, and cobalt chloride on micropropagation and biochemical parameters in the cherry rootstocks CAB-6P and Gisela 6. Turk J Biol 40:670-683. https://doi.org/10.3906/biy-1505-92   DOI
24 Vibha J. B., Shekhawat, N. S., Mehandru, P., & Dinesh, R. (2014). Rapid multiplication of Dalbergia sissoo Roxb.: A timb er yielding tree legume through axillary shoot proliferation and Ex vitro rooting. Physiology and Molecular Biology of Plants, 20(1), 81-87. https://doi.org/10.1007/s12298-013-0213-3   DOI
25 Husain MK, Anis M, Shahzad A (2008) In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium Roxb.) using nodal explants. Acta Physiol Plant 30:353-359   DOI
26 Ahmad Z, Shahzad A, Sharma S, Parveen S (2018) Ex vitro rescue, physiochemical evaluation, secondary metabolite production and assessment of genetic stability using DNA based molecular markers in regenerated plants of Decalepis salicifolia (Bedd. ex Hook. f.) Venter. Plant Cell Tissue Organ Cult 132:497-510   DOI
27 Beyer EM (1975) Abscission: the initial effect of ethylene is in the leaf blade. Plant Physiol 55:322-327   DOI
28 Beyer EM (1976a) A potent inhibitor of ethylene action in plants. Plant Physiol 58:268-271   DOI
29 Burg SP (1968) Ethylene, plant senescence and abscission. Plant Physiol 43:1503-1511
30 Chauhan M., Thakur, A., Singh, Y. et al. (2021) Gnotobiotic evaluation of Dalbergia sissoo genotypes for resistance against Fusarium solani via dual culture set up. J Genet Eng Biotechnol 19, 35. https://doi.org/10.1186/s43141-021-00132-3   DOI
31 Currais L, Loureiro J, Santos C, Canhoto JM (2013) Ploidy stability in embryogenic cultures and regenerated plantlets of tamarillo. Plant Cell Tissue Organ Cult 114:149-159   DOI
32 Chand S, Singh AK (2004) Plant regeneration from encapsulated nodal segments of Dalbergia sissoo Roxb., a timber-yielding leguminous tree species. J Plant Physiol 161:237-243   DOI
33 Naik SK, Chand PK (2003) Silver nitrate and aminoethoxyvinylglycine promote in vitro adventitious shoot regeneration of pomegranate (Punica granatum L.). J Plant Physiol 160:423-430   DOI
34 Pattnaik S, Pradhan C, Naik SK, Chand PK (2000) Shoot organogenesis and plantlet regeneration from hypocotyl-derived cell suspensions of a tree legume Dalbergia sissoo. In vitro Cell Dev Biol - Plant 36:407-411   DOI
35 Datta SK and Datta K (1983) Auxin induced regeneration of forest treesDalbergia sissoo Roxb. through tissue culture. Curr Sci 52:435-436
36 Beyer EM (1979) [14C] Ethylene metabolism during leaf abscission in cotton. Plant Physiol 64:971-974. https://doi.org/10.1104/pp.64.6.971   DOI
37 Shahzad A. et al. (2017) Plant Tissue Culture: Applications in Plant Improvement and Conservation. In Plant Biotechnology: Principles and Applications (pp. 37-72). Springer Singapore
38 Tamimi SM (2015) Effects of ethylene inhibitors, silver nitrate (AgNO3), cobalt chloride (CoCl2) and aminooxy acetic acid (AOA), on in vitro shoot induction and rooting of banana (Musa acuminata L.). Afr J Biotechnol 14:2511-2516. https://doi.org/10.5897/AJB2015.14788   DOI