• Title/Summary/Keyword: Micro-propagation

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Observation of Tribologically Transformed Structures and fretting Wear Characteristics of Nuclear Fuel Cladding (핵연료 봉의 마찰변태구조 관찰과 프레팅 마멸 특성)

  • Kim, Kyeong-Ho;Lee, Min-Ku;Rhee, Chang-Kyu;Wey, Myeong-Yong;Kim, Whung-Whoe
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.26 no.12
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    • pp.2581-2589
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    • 2002
  • In this research, fretting tests were conducted in air to investigate the wear characteristics of fuel cladding materials with the fretting parameters such as normal load, slip amplitude, frequency and the number of cycles. A high frequency fretting wear tester was designed for this experiment by KAERI. After the experiments, the wear volume and the shape of wear contour were measured by the surface roughness tester. Tribologically transformed structures(TTS) were analysed by means of optical and scanning electron microscopes to identify the main wear mechanisms. The results of this study showed that the wear volume were increased with increasing slip amplitude, and the shape of wear contour was transformed V-type to W-type. Also, it was found that the critical slip amplitude was 168${\mu}{\textrm}{m}$. These phenomena mean that wear mechanism transformed partial slip to gross slip to accelerate wear volume. The wear depth increased with an increase of friction coefficient due to increase of normal load and frequency. The fretting wear mechanisms were believed that, after adhesion and surface plastic deformation occurred by relative sliding motion on the contact between two specimens, TTS creation was induced by surface strain hardening and wear debris were detached from the contact surface which were produced by the micro crack propagation and creation.

Micropropagation of Superior Variety of Japanese Pepper Tree (Zanthoxylum piperitum Dc.) (수형목 민초피나무의 기내 대량 증식)

  • Sung Ho SON;Seong Doo HUR;Jung Hee KIM;Yun Hee LEE;Mee Hee KIM;Jin Seon PARK;Young Wook LEE;Heung Kyu MOON;Yang YOUN
    • Korean Journal of Plant Tissue Culture
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    • v.22 no.3
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    • pp.127-130
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    • 1995
  • Japanese pepper (Zanthoxylum piperitum Dc.) tree of selected genotype was propagated by a two-step method. Among the media tested, BTM promoted shoot height growth and DKW revealed as superior to micro-canopy increment Multiple shoot formation was greatest when the single shoot were subcultured on medium containing 0.89 $\mu$M BA alone. The numbers of survival shoots could be markedly increased by acclimatization of the multiplied shoots itself in plastic Petridish (punctured with pin on the top) for two weeks and subsequently transplanting each shoot onto peat plug system. After transplanting the micropropagules onto pots, prickliness characteristics seem to be transmitted to all plane produced from selected genotype.

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Adventitious Root Cultures of Panax ginseng C.V. Meyer and Ginsenoside Production through Large-Scale Bioreactor System

  • Hahn, Eun-Joo;Kim, Yun-Soo;Yu, Kee-Won;Jeong, Cheol-Seung;Paek, Kee-Yoeup
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.1-6
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    • 2003
  • The adventitious root of Panax ginseng C.A. Meyer is regarded as an efficient alternative to cell culture or hairy root culture for biomass production due to its fast growth and stable metabolite production. To determine optimal culture conditions for the bioreactor culture of ginseng roots, experiments have been conducted on physical and chemical factors such as bioreactor type, dissolved oxygen, gas supply, aeration, medium type, macro- and micro-elements, medium supplement during culture period, sucrose concentration, osmotic agents, medium pH and light. Elicitation is a key step to increase ginsenoside accumulation in the adventitious roots but biomass growth is severely inhibited by elicitor treatment. To obtain high ginsenoside content with avoiding biomass decrease, we applied two-stage bioreactor culture system. Ginseng adventitious roots were cultured for 40 days to maximize biomass increase followed by elicitation for 7 days to enhance ginsenoside accumulation. We also experimented on types and concentrations of jasmonate to determine optimal elicitation methods. In this paper, we discussed several factors affecting the root propagation and ginsenoside accumulation. Based on the results obtained from previous experiments we have established large-scale bioreactor system (1 ton-10 ton) for the efficient production of ginseng adventitious roots and bioactive compounds including ginsenoside. Still, experiments are on going in our laboratory to determine other bioactive compounds having effects on diet, high blood pressure, DPPH elimination and increasing memories.

Compressive resistance behavior of UHPFRC encased steel composite stub column

  • Huang, Zhenyu;Huang, Xinxiong;Li, Weiwen;Zhang, Jiasheng
    • Steel and Composite Structures
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    • v.37 no.2
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    • pp.211-227
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    • 2020
  • To explore the feasibility of eliminating the longitudinal rebars and stirrups by using ultra-high-performance fiber reinforcement concrete (UHPFRC) in concrete encased steel composite stub column, compressive behavior of UHPFRC encased steel stub column has been experimentally investigated. Effect of concrete types (normal strength concrete, high strength concrete and UHPFRC), fiber fractions, and transverse reinforcement ratio on failure mode, ductility behavior and axial compressive resistance of composite columns have been quantified through axial compression tests. The experimental results show that concrete encased composite columns with NSC and HSC exhibit concrete crushing and spalling failure, respectively, while composite columns using UHPFRC exhibit concrete spitting and no concrete spalling is observed after failure. The incorporation of steel fiber as micro reinforcement significantly improves the concrete toughness, restrains the crack propagation and thus avoids the concrete spalling. No evidence of local buckling of rebars or yielding of stirrups has been detected in composite columns using UHPFRC. Steel fibers improve the bond strength between the concrete and, rebars and core shaped steel which contribute to the improvement of confining pressure on concrete. Three prediction models in Eurocode 4, AISC 360 and JGJ 138 and a proposed toughness index (T.I.) are employed to evaluate the compressive resistance and post peak ductility of the composite columns. It is found that all these three models predict close the compressive resistance of UHPFRC encased composite columns with/without the transverse reinforcement. UHPFRC encased composite columns can achieve a comparable level of ductility with the reinforced concrete (RC) columns using normal strength concrete. In terms of compressive resistance behavior, the feasibility of UHPFRC encased steel composite stub columns with lesser longitudinal reinforcement and stirrups has been verified in this study.

Use of Ultrasonic beam transmissivity for investigating the structural features in plastic pipe cased borehole (초음파의 매질 투과성을 이용한 시추공 케이싱 배면의 암상 및 절리구조 조사 연구)

  • 김중열;김유성
    • Proceedings of the Korean Geotechical Society Conference
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    • 2002.03a
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    • pp.751-758
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    • 2002
  • Boreholes that are drilled in soft or unconsolidated materials such as gravels and coals are prone to collapse. To maintain the hole, some kinds of casing pipes are needed. If thereby a plastic pipe e.g. PVC is used for the casing, Televiewer tool is still capable of detecting structural features such as fractures in the borehole wall behind the pipe, whereas other borehole-imaging logging devices such as BIPS (Borehole Image Processing System) and FMS(Formation Micro Scanner) won't provide any information about that. Televiewer's primary component is a piezoelectric transducer centered in the hole. It acts as both a transmitter and receiver, and sends an ultrasonic beam. That is reflected, in the same manner as the seismic wave propagation, from the both sides(inner and outer surfaces) of the casing pipe, transmits through the pipe and then reflected from the borehole wall. With an appropriate choice of time-windowing, it is possible to capture the returning signals from both the borehole wall and the outer side of casing pipe as well. A suite of laboratory tests were performed on various physical models composed of plastic pipes with different diameters. Although the amplitudes of returning signals were reduced to about half the usual value due to the transmission loss, the dynamic range of Televiewer tool was sufficient to observe the structural features behind the casing pipe. Besides, several representative case studies at various research areas in our country are presented. The results demonstrate the usefulness of the transmissivity of Televiewer acoustic km, which will assist in further structural interpretation.

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System identification of soil behavior from vertical seismic arrays

  • Glaser, Steven D.;Ni, Sheng-Huoo;Ko, Chi-Chih
    • Smart Structures and Systems
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    • v.4 no.6
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    • pp.727-740
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    • 2008
  • A down hole vertical seismic array is a sequence of instruments installed at various depths in the earth to record the ground motion at multiple points during an earthquake. Numerous studies demonstrate the unique utility of vertical seismic arrays for studying in situ site response and soil behavior. Examples are given of analyses made at two sites to show the value of data from vertical seismic arrays. The sites examined are the Lotung, Taiwan SMART1 array and a new site installed at Jingliao, Taiwan. Details of the installation of the Jingliao array are given. ARX models are theoretically the correct process models for vertical wave propagation in the layered earth, and are used to linearly map deeper sensor input signals to shallower sensor output signals. An example of Event 16 at the Lotung array is given. This same data, when examined in detail with a Bayesian inference model, can also be explained by nonlinear filters yielding commonly accepted soil degradation curves. Results from applying an ARMAX model to data from the Jingliao vertical seismic array are presented. Estimates of inter-transducer soil increment resonant frequency, shear modulus, and damping ratio are presented. The shear modulus varied from 50 to 150 MPa, and damping ratio between 8% and 15%. A new hardware monitoring system - TerraScope - is an affordable 4-D down-hole seismic monitoring system based on independent, microprocessor-controlled sensor Pods. The Pods are nominally 50 mm in diameter, and about 120 mm long. An internal 16-bit micro-controller oversees all aspects of instrumentation, eight programmable gain amplifiers, and local signal storage.

Effect of Cobalt Contents on the Microstructure and Charpy Impact Properties of Ferritic/martensitic Oxide Dispersion Strengthened Steel (페라이트/마르텐사이트계 산화물분산강화강의 미세조직 및 샤르피 충격특성에 미치는 코발트 함량의 영향)

  • Kwon, Daehyun;Noh, Sanghoon;Lee, Jung Gu
    • Journal of Powder Materials
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    • v.27 no.4
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    • pp.311-317
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    • 2020
  • In this study, the effects of Co content on the microstructure and Charpy impact properties of Fe-Cr-W ferritic/martensitic oxide dispersion strengthened (F/M ODS) steels are investigated. F/M ODS steels with 0-5 wt% Co are fabricated by mechanical alloying, followed by hot isostatic pressing, hot-rolling, and normalizing/tempering heat treatment. All the steels commonly exhibit two-phase microstructures consisting of ferrite and tempered martensite. The volume fraction of ferrite increases with the increase in the Co content, since the Co element considerably lowers the hardenability of the F/M ODS steel. Despite the lowest volume fraction of tempered martensite, the F/M ODS steel with 5 wt% Co shows the highest micro-Vickers hardness, owing to the solid solution-hardening effect of the alloyed Co. The high hardness of the steel improves the resistance to fracture initiation, thereby resulting in the enhanced fracture initiation energy in a Charpy impact test at - 40℃. Furthermore, the addition of Co suppresses the formation of coarse oxide inclusions in the F/M ODS steel, while simultaneously providing a high resistance to fracture propagation. Owing to these combined effects of Co, the Charpy impact energy of the F/M ODS steel increases gradually with the increase in the Co content.

A Study on Microstructures and Cryogenic Mechanical Properties of Electron Beam Welds between Cast and Forged Inconel 718 Superalloys for Liquid Rocket Combustion Head (액체로켓 연소기용 Inconel 718 주조 및 단조 합금의 전자빔 용접부 미세조직 및 극저온 특성)

  • Hong, Hyun-Uk;Bae, Sang-Hyun;Kwon, Soon-Il;Lee, Je-Hyun;Do, Jeong-Hyeon;Choi, Baig-Gyu;Kim, In-Soo;Jo, Chang-Yong
    • Journal of Welding and Joining
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    • v.31 no.6
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    • pp.50-57
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    • 2013
  • Characterization of microstructures and cryogenic mechanical properties of electro beam (EB) welds between cast and forged Inconel 718 superalloys has been investigated. Optimal EBW condition was found in the beam current range of 36~39 mA with the constant travel speed of 12 mm/s and arc voltage of 120 kV for 10 mm-thick specimens. Electron beam current lower than 25 mA caused to occur the liquation microfissuring in cast-side heat affected zone (HAZ) of EB welds. The HAZ liquation microfissure was found on the liquated grain boundaries with resolidified ${\gamma}/Laves$ and ${\gamma}/NbC$ eutectic constituents. EBW produced welds showing a fine dendritic structure with relatively discrete Laves phase due to fast cooling rate. After post weld aging treatment, blocky Laves phase and formation of ${\gamma}^{\prime}+{\gamma}^{{\prime}{\prime}}$ strengtheners were observed. Presence of primary strengthener and coarse Laves particles in PWHT weld may cause to reduce micro-plastic zone ahead of a crack, leading to a significant decrease in Charpy impact toughness at $-196^{\circ}C$. Fracture initiation and propagation induced by Charpy impact testing were discussed in terms of the dislocation structures ahead of crack arisen from the fractured Laves phase.

Model test on slope deformation and failure caused by transition from open-pit to underground mining

  • Zhang, Bin;Wang, Hanxun;Huang, Jie;Xu, Nengxiong
    • Geomechanics and Engineering
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    • v.19 no.2
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    • pp.167-178
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    • 2019
  • Open-pit (OP) and underground (UG) mining are usually used to exploit shallow and deep ore deposits, respectively. When mine deposit starts from shallow subsurface and extends to a great depth, sequential use of OP and UG mining is an efficient and economical way to maintain mining productivity. However, a transition from OP to UG mining could induce significant rock movements that cause the slope instability of the open pit. Based on Yanqianshan Iron Mine, which was in the transition from OP to UG mining, a large-scale two-dimensional (2D) model test was built according to the similar theory. Thereafter, the UG mining was carried out to mimic the process of transition from OP to UG mining to disclose the triggered rock movement as well as to assess the associated slope instability. By jointly using three-dimensional (3D) laser scanning, distributed fiber optics, and digital photogrammetry measurement, the deformations, movements and strains of the rock slope during mining were monitored. The obtained data showed that the transition from OP to UG mining led to significant slope movements and deformations that can trigger catastrophic slope failure. The progressive movement of the slope could be divided into three stages: onset of micro-fracture, propagation of tensile cracks, and the overturning and/or sliding of slopes. The failure mode depended on the orientation of structural joints of the rock mass as well as the formation of tension cracks. This study also proved that these non-contact monitoring technologies were valid methods to acquire the interior strain and external deformation with high precision.

A self-confined compression model of point load test and corresponding numerical and experimental validation

  • Qingwen Shi;Zhenhua Ouyang;Brijes Mishra;Yun Zhao
    • Computers and Concrete
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    • v.32 no.5
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    • pp.465-474
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    • 2023
  • The point load test (PLT) is a widely-used alternative method in the field to determine the uniaxial compressive strength due to its simple testing machine and procedure. The point load test index can estimate the uniaxial compressive strength through conversion factors based on the rock types. However, the mechanism correlating these two parameters and the influence of the mechanical properties on PLT results are still not well understood. This study proposed a theoretical model to understand the mechanism of PLT serving as an alternative to the UCS test based on laboratory observation and literature survey. This model found that the point load test is a self-confined compression test. There is a compressive ellipsoid near the loading axis, whose dilation forms a tensile ring that provides confinement on this ellipsoid. The peak load of a point load test is linearly positive correlated to the tensile strength and negatively correlated to the Poisson ratio. The model was then verified using numerical and experimental approaches. In numerical verification, the PLT discs were simulated using flat-joint BPM of PFC3D to model the force distribution, crack propagation and BPM properties' effect with calibrated micro-parameters from laboratory UCS test and point load test of Berea sandstones. It further verified the mechanism experimentally by conducting a uniaxial compressive test, Brazilian test, and point load test on four different rocks. The findings from this study can explain the mechanism and improve the understanding of point load in determining uniaxial compressive strength.