• Title/Summary/Keyword: Micro-propagation

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Early Detection of Micro-Defects(Degradation) by Using Nonlinear Acoustic Effect (비선형 음향 효과를 이용한 미세 결함(열화)의 조기 검출)

  • Jhang, K.Y.;Kim, K.C.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.18 no.5
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    • pp.365-372
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    • 1998
  • The method of measuring the nonlinear effect of ultrasonic waves is suggested as a new approach for the effective evaluation of material degradation. In sonic wave propagation, the existence of nonlinear effect can be demonstrated by the generation of higher order harmonic waves. So, at first, the mechanism of generating higher order harmonic components due to nonlinear effect was explained by using nonlinear elasticity. Next, we attempted to measure how much of the higher order harmonic component was generated in the degraded material. For this purpose, a measurement system mainly based on a high-powered nonlinear ultrasonic signal analysis system was constructed, and SS41 and SS45 specimen intentionally degraded by tensile load and fatigue load were tested. From the results, we confirmed that the measurement of nonlinear acoustic effect may be useful for the evaluation of material degradation.

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Experimental and numerical studies of precast connection under progressive collapse scenario

  • Joshi, Digesh D.;Patel, Paresh V.;Rangwala, Husain M.;Patoliya, Bhautik G.
    • Advances in concrete construction
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    • v.9 no.3
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    • pp.235-248
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    • 2020
  • Progressive collapse in a structure occurs when load bearing members are failed and the adjoining structural elements cannot resist the redistributed forces and fails subsequently, that leads to complete collapse of structure. Recently, construction using precast concrete technology is adopted increasingly because it offers many advantages like faster construction, less requirement of skilled labours at site, reduced formwork and scaffolding, massive production with reduced amount of construction waste, better quality and better surface finishing as compared to conventional reinforced concrete construction. Connections are the critical elements for any precast structure, because in past, major collapse of precast structure took place because of connection failure. In this study, behavior of four different precast wet connections with U shaped reinforcement bars provided at different locations is evaluated. Reduced 1/3rd scale precast beam column assemblies having two span beam and three columns with removed middle column are constructed and examined by performing experiments. The response of precast connections is compared with monolithic connection, under column removal scenario. The connection region of test specimens are filled by cast-in-place micro concrete with and without polypropylene fibers. Performance of specimen is evaluated on the basis of ultimate load carrying capacity, maximum deflection at the location of removed middle column, crack formation and failure propagation. Further, Finite element (FE) analysis is carried out for validation of experimental studies and understanding the performance of structural components. Monolithic and precast beam column assemblies are modeled using non-linear Finite Element (FE) analysis based software ABAQUS. Actual experimental conditions are simulated using appropriate boundary and loading conditions. Finite Element simulation results in terms of load versus deflection are compared with that of experimental study. The nonlinear FE analysis results shows good agreement with experimental results.

The Characteristics of Ultrasonic Signals for Detecting Micro-Defects in Ti-6Al-4V Alloy (Ti-6Al-4V 합금의 내부 미소결함에 따른 초음파 신호 특성 연구)

  • Choi, Sang-Woo;Lee, Joon-Hyun;Kubota, M.;Murakami, Y.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.21 no.6
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    • pp.591-597
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    • 2001
  • Ti alloy is used for essential parts of aircraft for high temperature environment. Although Ti alloy has excellent performance in regard to mechanical properties, it is difficult ot find fatigue cracks by nondestructive ultrasonic inspection due to its two-phase microstructure, which consists of hard alpha and beta phases. Sound energy reflected from microstructural features in the component produces a background inspection noise which is seen even when no defects are present. This noise can inhibit the detection of critical internal defects such as pores cracks or inclusions. To obtain fundamental data on ultrasonic inspection of Ti alloy, ultrasonic testing was performed using a specimen with small drill holes and ultrasonic wave propagation velocites were measured.

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Effects of Carbon and Nitrogen Sources on the Shoot Formation in bioreator culture of Scrophularia buergeriana Miquel (현삼에서 탄소원과 질소원의 종류와 농도가 기내 식물체 분화에 미치는 영향)

  • Lim, Wan-Sang;Chae, Young-Am
    • Korean Journal of Medicinal Crop Science
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    • v.8 no.1
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    • pp.9-13
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    • 2000
  • To determine the proper carbon and nitrogen sources and their proper levels for mass micro propagation of Scrophularia buergeriana Miquel, tonic and curing cough experiment were applied and a method for mass cultivation by using bioreactors (2.5 L) was expinined. Proper ratio of $NH_4NO_3\;:\;$KNO_3$ was 413 mg/L : 1900 mg/L for multiple shoot production. Sucrose was more effective than glucose or fractose as carbon source and 3% concentration was good for shoot formation. Total nitrogen was not detected after six weeks both in 500 ml flask and bioreactor culture. Sucrose was decreased sharply after two weeks and there was no sucrose left after three weeks both in 500 ml flask and bioreactor culture. The stirrer in bioreactor caused shear stress to shoots severely. The sphere type bioreactor was better than the cylinder type and removal of inner loop in sphere type was more effective to avoid shear stress.

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Depth Sizing of Notch Fatigue Crack Using Diffracted Ultrasonic Wave (회절초음파를 이용한 노치 피로균열의 균열깊이 평가)

  • Jin, Mei-Ling;Lee, Tae-Hun;Park, Byung-Jun;Jhang, Kyung-Young
    • Journal of the Korean Society for Nondestructive Testing
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    • v.29 no.5
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    • pp.405-414
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    • 2009
  • This paper proposed a methodology based on ultrasonic diffraction technique to inspect the depth of a crack initiated from a notch of CT specimen by fatigue test, and its usefulness was verified by experiments. Especially, in order to identify accurately the diffractive waves from the crack tip in the situation where there are extra diffractive elements such as a notch, we have tried imaging by transducer scan and analyzed the propagation path of diffracted wave. Two specimens with and without a crack were experimented. Higher frequency and larger refractive angle of transducer showed a tendency to decrease the error in the measurements, and the measured crack depth showed an error less than 0.38 mm in case of 4 MHz $60^{\circ}-60^{\circ}$. The proposed methodology is applicable to weak diffractive sources, and so that it would be useful to inspect micro cracks and for their depth sizing.

Characteristics of Developmental Stages in Bacterial Biofilm Formation (세균 생물막 형성의 단계별 특징)

  • Kim Chang-Beom;Rho Jong-Bok;Lee Hyun-Kyung;Choi Sang Ho;Lee Dong-Hun;Park Soon-Jung;Lee Kyu-Ho
    • Microbiology and Biotechnology Letters
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    • v.33 no.1
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    • pp.1-8
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    • 2005
  • Since Anton van Leeuwen­hoek first observed a surface-associated multicellular structure of bacterial cells in the 17th century, it has been shown to exhibit an ability to form a biofilm by numerous bacterial species. The biofilm formation is composed of distinct developmental stages, which include an attachment/adhesion of a single cell, a proliferation toward monolayered coverage, a propagation to aggregated microcolony, a maturation to 3-dimensional structure, and subsequently a local degradation. Investigation to identify the essential factors for bacterial biofilm formation has been performed via classical genetic approaches as well as recently developed technologies. The initial stage requires bacterial motility provided by a flagellum, and outermembrane components for surface signal interaction. Type IV-pilus and autoaggregation factors, e.g., type I-fimbriae or Ag43, are necessary to reach the stages of monolayer and micro colony. The mature biofilm is equipped with extracellular polymeric matrix and internal water-filled channels. This complex architecture can be achieved by differential expressions of several hundred genes, among which the most studied are the genes encoding exopolysaccharide biosyntheses and quorum-sensing regulatory components. The status of our knowledge for the biofilms found in humans and natural ecosystems is discussed in this minireview.

Defect Monitoring of a Wind Turbine Blade Surface by using Surface Wave Damping (표면파 기반의 풍력발전기 블레이드 표면상태 실시간 모니터링에 관한 연구)

  • Kim, Kyung-Hwan;Yang, Young-Jin;Kim, Hyun-Bum;Yang, Hyung-Chan;Lim, Jong-Hwan;Choi, Kyung-Hyun
    • Clean Technology
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    • v.23 no.1
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    • pp.90-94
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    • 2017
  • These days much efforts are being dedicated to wind power as a potential source of renewable energy. To maintain effective and uniform generation of energy, defect preservation of turbine blade is essential because it directly takes effects on the efficiency of power generation. For the effective maintenance, early measurements of blade defects are very important. However, current technologies such as ultrasonic waves and thermal imaging inspection methods are not suitable because of long inspection time and non-real time inspection. To supplement the problems, the study introduced a method for real time defect monitoring of a blade surface based on surface wave technology. We examined the effect of various parameters such as micro-cracks and peelings on the propagation of surface wave.

A CONTROLLED CYCLIC LOADING ON THE SURFACE TREATED AND BONDED CERAMIC: STAIRCASE METHOD

  • Yi, Yang-Jin
    • The Journal of Korean Academy of Prosthodontics
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    • v.46 no.3
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    • pp.298-306
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    • 2008
  • STATEMENT OF PROBLEM: Effect of surface treatment of ceramic under loading does not appear to have been investigated. PURPOSE: The aim of this study was to investigate the effect of surface treatment of esthetic ceramic, which is performed to increase the bonding strength, on the fracture stress under controlled cyclic loading condition. MATERIAL AND METHODS: Sixty 1.0 mm-thick specimens were made from Mark II Vitablocs (Vita Zahnfabrik, Germany) and divided into 3 groups: polished (control), sandblasted, and etched. Specimens of each group were bonded to a dentin analog material base including micro-channels to facilitate the flow of water to the bonding interface. Bonded ceramics were cyclically loaded with a flat-end piston in the water (500,000 cycles, 15Hz). Following completion of cyclic loading, specimens were examined for subsurface crack formation and subsequent stress was determined and loaded to next specimen by the staircase method according to the crack existence. RESULTS: There were significant differences of mean fatigue limit in the sandblasted (222.86 ${\pm}$ 23.42 N) and etched group (222.86 ${\pm}$ 14.16 N) when compared to polished group (251.43 ${\pm}$ 10.6 N) (P<.05; Wald-type pair-wise comparison and post hoc Bonferroni test). Of cracked specimens, surface treated group showed longer crack propagation after 24 hours. All failures originated from the radial cracking without cone crack. Fracture resistance of this study was very low and comparable to failure load in the oral cavity. CONCLUSION: Well controlled cyclic loading could induce clinically relevant cracks and fracture resistance of Mark II ceramic was relatively low applicable only to anterior restorations. Surface treatment of inner surface of feldspathic porcelain in the matsicatory area could influence lifetime of restorations.

System Capacity and Coverage Analysis of Hierarchical Femtocell Networks (펨토셀 기반 계층셀 구조 시스템 용량 및 서비스 반경 분석)

  • O, Nam-Geol;Kim, Hoon
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.6A
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    • pp.476-483
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    • 2009
  • Recently much attention has been devoted to femtocell's potential to improve indoor cellular coverage and high speed wireless communications. Femtocell based commercial services have been already launched in some countries and standardization activities are actively on-going, there has been concern however over potential issues of interference between femtocells and the micro/macro networks. With universal frequency reuse, the ensuing cross-tier interference causes unacceptable data rate and outage probability, so an analysis of effect of interference in femtocell embedded networks would be necessary for a stable system design. This paper investigates the effect of interference on system performances of femtocell embedded hierarchical cell structure (HCS) networks considering the characteristics of propagation environments. Various channel parameters are specially considered for indoor environments where most of femtocells are deployed to investigate the effect of interference of femtocell embedded RCS networks. System capacity and coverage are provided with variant distance between macrocell and femtocell, location of the user in femtocell coverage, and characteristic of building structures.

Observation of Tribologically Transformed Structures and fretting Wear Characteristics of Nuclear Fuel Cladding (핵연료 봉의 마찰변태구조 관찰과 프레팅 마멸 특성)

  • Kim, Kyeong-Ho;Lee, Min-Ku;Rhee, Chang-Kyu;Wey, Myeong-Yong;Kim, Whung-Whoe
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.26 no.12
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    • pp.2581-2589
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    • 2002
  • In this research, fretting tests were conducted in air to investigate the wear characteristics of fuel cladding materials with the fretting parameters such as normal load, slip amplitude, frequency and the number of cycles. A high frequency fretting wear tester was designed for this experiment by KAERI. After the experiments, the wear volume and the shape of wear contour were measured by the surface roughness tester. Tribologically transformed structures(TTS) were analysed by means of optical and scanning electron microscopes to identify the main wear mechanisms. The results of this study showed that the wear volume were increased with increasing slip amplitude, and the shape of wear contour was transformed V-type to W-type. Also, it was found that the critical slip amplitude was 168${\mu}{\textrm}{m}$. These phenomena mean that wear mechanism transformed partial slip to gross slip to accelerate wear volume. The wear depth increased with an increase of friction coefficient due to increase of normal load and frequency. The fretting wear mechanisms were believed that, after adhesion and surface plastic deformation occurred by relative sliding motion on the contact between two specimens, TTS creation was induced by surface strain hardening and wear debris were detached from the contact surface which were produced by the micro crack propagation and creation.