• Molecules and Cells
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• 제38권10호
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• pp.895-903
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• 2015

Non-coding microRNAs (miRNAs) regulate the translation of target messenger RNAs (mRNAs) involved in the growth and development of a variety of cells, including primordial germ cells (PGCs) which play an essential role in germ cell development. However, the target mRNAs and the regulatory networks influenced by miRNAs in PGCs remain unclear. Here, we demonstrate a novel miRNAs control PGC development through targeting mRNAs involved in various cellular pathways. We reveal the PGC-enriched expression patterns of nine miRNAs, including miR-10b, -18a, -93, -106b, -126-3p, -127, -181a, -181b, and -301, using miRNA expression analysis along with mRNA microarray analysis in PGCs, embryonic gonads, and postnatal testes. These miRNAs are highly expressed in PGCs, as demonstrated by Northern blotting, miRNA in situ hybridization assay, and miRNA qPCR analysis. This integrative study utilizing mRNA microarray analysis and miRNA target prediction demonstrates the regulatory networks through which these miRNAs regulate their potential target genes during PGC development. The elucidated networks of miRNAs disclose a coordinated molecular mechanism by which these miRNAs regulate distinct cellular pathways in PGCs that determine germ cell development.

• The Korean Journal of Physiology and Pharmacology
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• 제23권6호
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• pp.449-458
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• 2019

Retinoblastoma (Rb) is one of the most common eye malignancies occur in childhood. The crucial roles of non-coding RNAs, particularly long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been widely reported in Rb progression. In the present study, we found the expression of lncRNA T-cell leukemia/lymphoma 6 (TCL6) was significantly downregulated in Rb tissues and cell lines. Knockdown of lncRNA TCL6 promoted cell proliferation while reduced cell apoptosis in Rb cells. Moreover, lncRNA TCL6 serves as a sponge for miR-21, a previously-reported oncogenic miRNA in Rb, by direct targeting to negatively regulated miR-21 expression, therefore modulating Rb proliferation through miR-21. TCL6 overexpression inhibited Rb cell proliferation while miR-21 overexpression exerted an opposing effect; the effect of TCL6 overexpression was partially attenuated by miR-21 overexpression. PTEN/PI3K/AKT signaling pathway was involved in lncRNA TCL6/miR-21 axis modulating Rb cell proliferation. Taken together, lncRNA TCL6 serves as a tumor suppressor by acting as a sponge for miR-21 to counteract miR-21-mediated PTEN repression.

• 생명과학회지
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• 제29권1호
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• pp.118-122
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• 2019

옥시토신(Oxt)과 바소프레신(Avp)은 주로 시상하부의 신경세포에서 생성이 되어 뇌하수체 후엽에서 온 몸으로 분비된다. 유전자 구조와 염기서열 연구를 통해 옥시토신과 바소프레신이 진화적으로 발달되는 단계에서 유전자가 염색체 내에 중복된 것으로 추정되어져 왔다. 이전 연구에서 작은 조절자로 알려진 마이크로RNA 중 하나인 miR-24가 옥시토신과 직접 결합한 후 조절할 수 있다는 사실을 본 연구실에서 발표한 바가 있지만, 바소프레신을 동시에 조절할 수 있는지는 확실치 않았다. 본 연구에서 바소프레신을 조절할 수 있는 후보 마이크로RNA를 생물정보학적 방법으로 탐색하였다. 여러 후보 중 miR-24만이 바소프레신과 직접 결합할 수 있음을 형광 리포터 분석과 바소프레신 결합부위의 돌연변이 cDNA 제작을 통해 밝혀내었다. 바소프레신의 miR-24 결합 필수 부위인 "seed" 부분을 돌연변이 시킨 바소프레신의 경우 miR-24와의 결합능이 현저히 떨어지고 miR-24의 저해제 역시 결합능을 감소시키는 것을 보아 miR-24가 바소프레신에 결합하여 조절할 수 있음을 명확히 할 수 있었다. 이러한 결과를 종합해 볼 때 단일 마이크로RNA가 두 주요한 뇌하수체 호르몬의 조절에 관여한다는 새로운 조절 기전을 제시하여 준다.

• 한국환경농학회지
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• 제40권3호
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• pp.186-193
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• 2021

BACKGROUND: Before generating transgenic plant using the CRISPR-Cas9 system, the efficiency test of sgRNAs is recommended to reduce the time and effort for plant transformation and regeneration process. The efficiency of the sgRNA can be measured through the transient expression of sgRNA and Cas9 gene in tomato cotyledon; however, we found that the calculated efficiency showed a large variation. It is necessary to increase the precision of the experiment to obtain reliable sgRNA efficiency data from transient expression. METHODS AND RESULTS: The cotyledon of 11^th, 15^th, 19^th, and 23^rd-day-old tomato (Solanum lycopersicum cv. Micro-Tom) were used for expressing CRISPR-Cas9 transiently. The agrobacterium harboring sgRNA for targeting ALS2 gene of tomato was injected through the stomata of leaf adaxial side and the genomic DNA was extracted in 5 days after injection. The target gene edition was identified by amplifying DNA fragment of target region and analyzing with Illumina sequencing method. The target gene editing efficiency was calculated by counting base deletion and insertion events from total target sequence read. CONCLUSION: The CRISPR-Cas9 editing efficiency varied with tomato cotyledon age. The highest efficiency was observed at the 19-day-old cotyledons. Both the median and mean were the highest at this stage and the sample variability was also minimized. We found that the transgene of CRISPR-Cas9 system was strongly correlated with plant leaf development and suggested the optimum cotyledon leaf age for Agrobacterium-mediated transfection in tomato.

• 연구윤리
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• 제2권1호
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• pp.19-24
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• 2021

Purpose: The purpose of this study is to check the direction of KODISA's pursuit of complex and convergence studies by confirming the research trends of KODISA's direct academic journals such as JDS, JIDB, JBEES and JAFEB. To this end, we tried to compare and confirm the research trends of the papers in four academic journals targeting keywords. Research Design, data and methodology: The analysis was conducted from 2014 to 2020 on 867 papers from JDS, 315 papers from JIDB, 120 papers from JBEES, and 867 papers based on the publication year of the most recently published journal from JAFEB. For the analysis, frequency analysis, word crowding, topic modeling, and frequency analysis by applying weights for each year group were performed on the keywords crawled using Python. Results: The results of frequency analysis showed that each journal is properly oriented toward its target direction. In addition, it was confirmed that the results of topic modeling significantly reflected the results of frequency analysis. Finally, it could be concluded that the results of frequency analysis using the weights of keywords by year group were also developing in the direction the target journals were analyzed. Specifically, in the case of JDS, 20 keywords such as Service Quality, Distribution were found to increase continuously according to the year group. Meanwhile, the keywords that continued to increase according to JIDB's year group were India, Social Capital, and Job Stress. The keywords that continued to increase according to the year group of JBEES were Micro Finance Institutions and Microfinance, and the keywords that of JAFEB were confirmed to be Vietnam and Service Quality. Conclusion: It was confirmed that KODISA's direct management journals responded appropriately to convergence issues. In particular, it was confirmed that researches in various fields of JDS are continuously increasing. However, it seems that JIDB needs to deal with various issues additionally in the service industry field and JBEES in the environment field. Finally, it was found that JAFEB needs to be wary of the relatively low level of interest in some countries such as Kazakhstan and India in recent years.

• 한국ITS학회 논문지
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• 제20권4호
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• pp.71-82
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• 2021

본 연구에서는 세종시 포장도로 구간을 대상으로 모바일매핑시스템(MMS)과 딥러닝 균열 감지시스템을 활용한 도로포장 모니터링 방안을 제시하고 경제성 분석을 통한 최적 유지보수 공법을 제시하였다. 나아가, 기존 대부분의 연구에서는 도로포장 조사 차량에 의해 취득된 데이터를 활용한 사례가 대부분이었으나 본 연구에서는 직접 모니터링 조사를 통해 취득한 도로 포장 상태등급을 기준으로 경제성 분석을 실시하였다. 도로포장 상태 모니터링 결과 일반국도, 시도, 지방도의 순서대로 도로포장 상태가 양호한 것을 확인하였다. 또한 포장파손모델을 활용한 경제성 분석 결과, 예방적 유지보수공법인 마이크로서페이싱 공법을 적용하는 것이 유지보수비용과 이용자 편익 측면에서 가장 경제적인 것으로 나타났다. 본 연구의 성과는 지자체의 기반시설 관리계획 수립을 위한 기초적 자료로 활용될 것으로 기대된다.

• The Korean Journal of Physiology and Pharmacology
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• 제27권3호
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• pp.197-208
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• 2023

Dysregulation of certain long non-coding RNAs may facilitate tumor initiation and progression. However, numerous carcinogenesis-related long noncoding RNAs have not been characterized. The goal of this study was to elucidate the role of LINC00562 in gastric cancer (GC). The expression of LINC00562 was analyzed using real-time quantitative PCR and Western blotting. The proliferative capacity of GC cells was determined using Cell Counting Kit-8 and colony-formation assays. The migration of GC cells were evaluated using wound-healing assays. The apoptosis of GC cells was assessed by measuring the expression levels of apoptosis-related proteins (Bax and Bcl-2). Xenograft models in nude mice were constructed for in vivo functional analysis of LINC00562. The binding relationship between miR-4636 and LINC00562 or adaptor protein complex 1 sigma 3 (AP1S3), obtained from public databases, was confirmed using dual-luciferase and RNA-binding protein immunoprecipitation experiments. LINC00562 was expressed in GC cells at high levels. Knockdown of LINC00562 repressed GC cell growth and migration, promoted apoptosis in vitro, and inhibited tumor growth in nude mouse models. LINC00562 directly targeted miR-4636, and miR-4636 depletion restored the GC cell behavior inhibited by LINC00562 absence. AP1S3, an oncogene, binds to miR-4636. MiR-4636 downregulation increased AP1S3 level, restoring GC cell malignant behaviors inhibited by AP1S3 downregulation. Thus, LINC00562 exerts carcinogenic effects on GC development by targeting miR-4636-mediated AP1S3 signaling.

• 생명과학회지
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• 제31권8호
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• pp.710-718
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• 2021

태반유래 중간엽줄기세포(PD-MSCs)는 재생의학에서 세포기반치료제로 잘 알려진 세포군이다. PD-MSCs의 손상된 부위로의 이동과 호밍 기능은 MSC 생착의 중요한 특성이다. miRNA는 최근 MSC의 증식, 생존 이동과 같은 중요한 기능을 조절하는 것으로 알려져 있다. 본 연구의 목적은 담관결찰(BDL) 쥐 모델에서 PD-MSCs 호밍에 관련된 miRNA 및 표적 유전자를 동정하는 것으로, 마이크로어레이 분석을 이용하여 PD-MSCs 호밍에 관여하는 유전자 표적 miRNA를 선별하였다. BDL 쥐모델에 PD-MSCs을 이식한 일주일 후 간 조직에서 PD-MSCs 생착여 부는 면역형광분석법과 qRT-PCR에 의한 인간 Alu유전자 발현으로 확인되었다. 저산소 및 정상조건(Hyp/Nor)에서 이동한 PD-MSC에 비하여, PD-MSCs 이식한 BDL군 간 조직에서 miRNAs 발현의 차이가 크게 나타났으며, PD-MSCs 호밍 관련 miRNA와 표적유전자를 검증하였다. miR199a-5p 및 miR-148a-3p에 대한 표적 유전자 인테그린 α4 (ITGA4)와 α5 (ITGA5)의 발현은 이식(Tx)그룹에서(p<0.05) 유의하게 상향 조절되었다. 또한 인테그린 β1 (ITGB1)과 β8 (ITGB8)의 발현은 miR-183-5p 및 miR-145-5p억제에 의하여 크게 증가되었다. 따라서 이러한 결과는 BDL에 의해 손상된 쥐간에서 PD-MSCs가 호밍효과을 위해 인테그린 그룹과 관련된 miRNA 발현 조절에 관여함을 나타내었다. 본 연구결과는 miRNA에 의한 인테그린 그룹 조절기능이 BDL에 의해 유도된 간섬유증 쥐모델에서 PD-MSCs의 치료효과에 기여할 수 있음을 시사한다.

• 디지털융복합연구
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• 제11권8호
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• pp.1-10
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• 2013

정치캠페인에서 유권자들의 표심(share of mind)을 읽는 중요성이 증가함에도 불구하고 인게이지먼트(engagement)를 정치캠페인 관점에서 분석한 연구가 없다. 따라서 본 연구의 목적은 인게이지먼트적 관점에서 정치캠페인의 후보자 몰입(immersion)에 대한 질적지표인 정치인게이지먼트 지수(Political Engagement Index : PEI)산출을 통해 정치광고홍보 전략을 위한 과학적 자료를 제공하고자 한다. 이를 위해 2012년 12월 19일 제 18대 대통령선거에 출마했던 A, B 두 후보자를 대상으로 삼았다. 설문지 응답 대상은 이번 대선에서 이외로 투표 참여율 낮은 젊은 층에게 설문하였다. 본 연구는 광고, 홍보, 정치여론과 시청률 등의 정량적 평가를 뛰어 넘어 질적 내용분석이 갖는 제한점을 보완하고자 하는 정성적 평가이다. 평가속성은 8개 유목에 PEI는 0~100점 사이에 분포하도록 설계하여 PEI 가 50 이상이면 보통 이상의 몰입, 50 이하면 보통 이하의 몰입도 의미하도록 했다. 본 연구의 모형은 정치캠페인전략의 방법론적 연구발전에 기여하게 될 것이다. 또한 향후 각 정치캠페인의 선거전략에서 이를 마이크로 타깃팅으로 활용할 수 있을 것이다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3503-3507
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• 2013

Background: Although the majority of investigations concerned with TP53 and its protein have focused on coding regions, recently a set of studies highlighted significant roles of regulatory elements located in p53 mRNA, especially 5'UTR. The wrap53${\alpha}$ transcript is one of those that acts as a natural antisense agent, forming RNA-RNA hybrids with p53 mRNA and protecting it from degradation. Materials and Methods: In this study, we focused on the mutation status of exon $1{\alpha}$ of the WRAP53 gene (according to exon 1 of p53) in 160 breast tumor tissue samples and conducted a bioinformatics search for probable miRNA binding site in the p53/wrap53 overlapping region. Mutations were detected, using single stranded conformation polymorphism (SSCP) and sequencing. We applied the miRBase database for prediction of miRNAs which target overlapping region of p53/wrap53 transcripts. Results: Our results showed all samples to have wild type alleles in exon 1 of TP53 gene. We could detect a novel and unreported intronic mutation (IVS1+56, G>C) outside overlapping regions of p53/wrap53 genes in breast cancer tissues and also predict the presence of a binding site for miR-4732-5p in the 5'UTR of Wrap53 mRNA. Conclusions: From our findings we propose designing further studies focused on overexpression of miRNA-4732-5p and introducing different mutations in the overlapping region of wrap53 and p53 genes in order to study their effects on p53 and its ${\Delta}N$ isoform (${\Delta}$40p53) expression. The results may provide new pieces in the p53 targeting puzzle for cancer therapy.