• Composites Research
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• v.32 no.5
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• pp.249-257
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• 2019

In this paper, the buckling behavior of triaxially braided circular arch with monosymmetric open section subjected to three-point bending was studied experimentally and numerically. First, test specimens were manufactured using vacuum assisted resin transfer molding (VARTM). Then the specimen was tested under three-point bending to determine the ultimate buckling strength. Before performing the numerical analysis, effective material properties of the braided composite were obtained through micro-meso scale analysis virtual testing validated with available test results. Then linear buckling analysis and geometrically non-linear post buckling analysis, established to simulate the test setup, were performed to study the buckling behavior of the composite frame. Analysis results were compared with experimentally obtained ones for verification. The effect of manufacturing defects of tow misalignment, irregular surface and resin rich region, and uncertainties during test setup were studied using numerical models. From the numerical analyses performed it was observed that both manufacturing defect and uncertainties had effect on the buckling behavior and strength.

• Journal of the korean academy of Pediatric Dentistry
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• v.36 no.3
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• pp.394-403
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• 2009

As the minimally invasive approach against white spot like early caries lesions in proximal surfaces of the teeth, therapeutic sealing has been introduced and studied for effective materials and methods to arrest the early caries lesion effectively, which is still going on. This study was performed for the purpose of evaluating its validity for the non-cavitated lesions according to the depth from surface using therapeutic sealing followed by artificial caries induction and evaluation with micro-CT, and we obtained the results as follows. 1. It was revealed that the deeper the caries lesions are, the lower radiation intensity at lesion body areas in pre-treatment specimen. 2. In the sealed groups, there were no differences in radiation intensity between pre- and post-treatment, whereas there were significant decreases in unsealed groups(p<0.05). 3. Even in the specimens with the lesions reaching deeply into DEJ, the effect of sealing was significant(p<0.05). Conclusively, it was thought therapeutic sealing can be an effective tool against the early caries lesions, regardless of their depth into tooth, unless cavitated.

• Journal of the Korean Society of Radiology
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• v.6 no.2
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• pp.93-98
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• 2012

Micro-computed tomography (microCT) is an important tool for preclinical vascular imaging, with micron-level resolution. This non-destructive means of imaging allows for rapid collection of 2D and 3D reconstructions to visualize specimens prior to destructive analysis such as pathological analysis. Objectives. The aim of this study was to suggest a method for ex vivo, postmortem examination of stented arterial segments with microCT. And ex vivo evaluation of stents such as bare metal or drug eluting stents on in-stent restenosis (ISR) in rabbit model was performed. The bare metal stent (BMS) and drug eluting stent (DES, paclitaxel) were implanted in the left or right iliac arteries alternatively in eight New Zealand white rabbits. After 4 weeks of post-implantation, the part of iliac arteries surrounding the stent were removed carefully and processed for microCT. Prior to microCT analysis, a contrast medium was loaded to lumen of stents. All samples were subjected to an X-ray source operating at 50 kV and 200 ${\mu}A$ by using a 3D isotropic resolution. The region of interest was traced and measured by CTAN analytical software. Objects being exposed to radiation had different Hounsfield unit each other with values of approximately 1.2 at stent area, 0.12 ~ 0.17 at a contrast medium and 0 ~ 0.06 at outer area of stent. Based on above, further analyses were performed. As a result, the difference of lengths and volumes between expanded stents, which may relate to injury score in pathological analysis, was not different significantly. Moreover, ISR area of BMS was 1.6 times higher than that of DES, indicating that paclitaxel has inhibitory effect on cell proliferation and prevent infiltration of restenosis into lumen of stent. And ISR area of BMS was higher ($1.52{\pm}0.48mm^2$) than that of DES ($0.94{\pm}0.42mm^2$), indicating that paclitaxel has inhibitory effect on cell proliferation and prevent infiltration of restenosis into lumen of stent. Though it was not statistically significant, it showed that the extent of neointema of mid-region of stents was relatively higher than that of anterior and posterior region in parts of BMS as showing cross-sectional 2-D image. suggest that microCT can be utilized as an accessorial tool for pathological analysis.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.34 no.1
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• pp.85-90
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• 2010

Friction welding is a common practice to join axially symmetrical parts for automobile industry applications. The shaft for automobile reverse idle gear is generally produced by forging steel, SF 45. This method is not so good because of high cost of material and production. In this study, in order to investigate the possibility of application of SM 45C to SF 45 dissimilar friction welding, the dissimilar friction welded joints were performed using 20 mm diameter solid bar in forging steel(SF 45) to carbon steel(SM 45C). The optimal friction welding parameters were selected to ensure reliable quality welds on the basis of visual examination, tensile test, micro-Virkers hardness surveys of the bond of area and optical microstructure investigations for welded joint parts. Finally, post weld heat treatment(PWHT) of the high-frequency induction hardening was performed for the friction welded specimens under the optimal welding conditions. And then, the mechanical properties were compared for as-welded and PWHT in SM 45C to SF 45.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.135-141
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• 2010

Embryoid bodies (EBs) generated from human embryonic stem cells (hESCs) include spontaneously induced endodermal lineage cells (ELCs). Activin-A plays important roles in the endoderm differentiation of hESCs. Despite studies on the generation of ELCs from hESCs with treatment of Actvin-A, it was unclear for localization and pattern of ELCs by Activin-A during differentiation of hESCs. Accordingly in this study, we knew that Actvin-A increased the cystic EBs formation, including the highly enriched AFP (endoderm lineage specific marker)-expressing cells in the surface of cystic EBs. To induce the EBs formation from undifferentiated hESCs, cells were transferred onto petri-dish and cultured in suspension condition without bFGF removed hESC media (EB media) for 3 days. Next to investigate the effect of Activin-A, EBs were subsequently cultured in EB media supplement with 100 ng/ml Activin-A for 3 days. After 5~7 days of Activin-A treatment, cystic EBs began to appear which increased in numbers reaching ~60% of initially formed EBs over 5 days. Endoderm lineage marker, AFP were highly expressed and specifically localized at the surface region of cystic EBs comparison with normal EBs. We next attached the cystic EBs onto gelatin-coated plates and cultured for 5 days. In the results of real-time PCR and immunocytochemistry analysis, AFP-expressing cells migrated and localized at the outgrowth region of attached cystic EBs. To obtain the AFP-expressing cells of the outgrowth region, we manually isolated by using micro-dissection and cultured them. These cells strongly express AFP over 70% of isolated cells post re-plating. Here, we first showed an expression pattern of specifically localized ELCs by Activin-A during differentiation of hESCs. From this observation, we could highly purified ELCs from undifferentiated hESCs. Taken together, our system will provide a novel and efficient option to generate ELCs from hESCs.

• Journal of agricultural medicine and community health
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• v.8 no.1
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• pp.19-27
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• 1983

This study was carried out to obtain the basic data for the prevention of chronic poisoning by organic phosphorous pesticides and to estimate the normal range of plasma cholinesterase activity level for Korean agricultural workers. The 285 agricultural workers were randomly selected for the study in Jungup Gun, Jeonbug Province, 135 persons of them were tested in the period of pre-exposure of organophosphorous pesticides (April 1981) and rest of them were tested after exposure(August 1981). Cholinesterase activity levels were measured by the Micro-method of Takahashi Hiroshi. Major findings are as following: 1) There is significant difference in plasma cholinesterase activity levels between the pro-exposed and post exposed group(p > 0.01). Mean values of plasma cholinesterase activity levels were $7.60{\pm}1.74{\mu}M/20{\mu}{\ell}$/hr. in the pre-exposed group and $6.23{\pm}1.59{\mu}M/20{\mu}{\ell}$/hr. in the post-exposed group. 2) Plasma cholinesterase activity in Korean agricultural worker could be estimated $7.30-7.90{\mu}M/20{\mu}{\ell}$/hr. with 95% confidence interval.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.3 no.4
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• pp.301-307
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• 2005

The morphology of the high burnup $UO_2$ spent fuel, which was oxidized and annealed in a PIA (Post Irradiation Annealing) apparatus, has been observed. The high burnup fuel irradiated in Ulchin Unit 2, average rod burnup 57,000 MWd/tU, was transported to the KAERI's PIEF. The test specimen was used with about 200 mg of the spent $UO_2$ fuel fragment of the local burnup 65,000 MWd/tU. This specimen was annealed at $1400^{\circ}C$ for 4hrs after the oxidation for 3hrs to grain boundary using the PIA apparatus in a hot-cell. In order to oxidize the grain boundary, the oxidation temperature increased up to $500^{\circ}C$ and held for 3hrs in the mixed gas (60 ml He and 100 ml STD-air) atmosphere. The amount of 85Kr during the whole test process was measured to know the fission gas release behavior using the online system of a beta counter and a gamma counter. The detailed micro-structure was observed by a SEM to confirm the change of the fuel morphology after this test. As the annealing temperature increased, the fission products were observed to move to the grain surface and grain boundary of the $UO_2$ matrix. This specimen was re-structured through the reduction process, and the grain sizes were distributed from 5 to $10\;{\mu}m$.

• Restorative Dentistry and Endodontics
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• v.34 no.3
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• pp.232-239
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• 2009

The aim of this study was to compare the effects of anti curvature filing with stainless steel k-file versus nickel-titanium ProFile in the shaping of mesial root canals of extracted mandibular molars. A total of 60 canals from 30 mesial roots of mandibular molar teeth were randomly assigned to three groups with n=20 each. They were prepared with different instruments and methods: The first group with stainless steel k-file and circumferential filing. the second with precurved stainless steel k-file and anticurvature filing and the third with ProFile C06 taper) and anticurvature filing. Using a micro-computed tomography system (skyscan-1076, SKYSCAN, Antwerpen, Belgium), pre-and post-operative specimens were scanned. Subsequently, canal images were superimposed and changes in root dentin thickness were measured at distal side (danger zone) of the canal. The data was analyzed using a one-way ANOVA and the comparison of means was conducted using a post hoc multiple comparison Tukey test. There were significant differences in the change of root dentin thickness at the $7.5{\sim}8.5\;mm$ level between group 1 and 2, $3.5{\sim}6mm$ level between group 1 and 3 and $3.5{\sim}6mm$ level between group 2 and 3(n=20, P<0.05).

• Restorative Dentistry and Endodontics
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• v.34 no.1
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• pp.69-79
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• 2009

The purpose of this study was to evaluate the influence of elastic modulus of restorative materials and the number of interfaces of post and core systems on the stress distribution of three differently restored endodontically treated maxillary second premolars using 3D FE analysis. Model 1, 2 was restored with a stainless steel or glass fiber post and direct composite resin. A PFG or a sintered alumina crown was considered. Model 3 was restored by EndoCrown. An oblique 500 N was applied on the buccal (Load A) and palatal (Load B) cusp. The von Mises stresses in the coronal and root structure of each model were analyzed using ANSYS. The elastic modulus of the definitive restorations rather than the type of post and core system was the primary factor that influenced the stress distribution of endodontically treated maxillary premolars. The stress concentration at the coronal structure could be lowered through the use of definitive restoration of high elastic modulus. The stress concentration at the root structure could be lowered through the use of definitive restoration of low elastic modulus.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.7
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• pp.954-960
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• 2008

MiRNAs (microRNAs) are a class of small non-coding RNA molecules of ~21 nucleotides that down- regulate the expression of target genes at post-transcriptional level. In this study, we first accomplished a preliminary scan of miRNA expression using 65 and 90 day fetal pig skeletal muscle samples by microarray hybridization, and 34 miRNAs showed strong positive signals. Five of these miRNAs were selected for further investigation by real-time RT-PCR. The statistical analyses indicated that three miRNAs exhibited significant differential expression (p<0.05) during porcine muscle development from 65 to 90 days of gestation, e.g., miR-24 and miR-424 were down-regulated while miR-133a was up-regulated. Multi-tissue RT-PCR was performed to detect the expression patterns of the five miRNA precursors. The results showed that most of these precursor miRNAs were ubiquitously expressed in different porcine tissues.