• Title/Summary/Keyword: Micro-CMM

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Detection of Clavibacter michiganensis subsp. michiganensis Assisted by Micro-Raman Spectroscopy under Laboratory Conditions

  • Perez, Moises Roberto Vallejo;Contreras, Hugo Ricardo Navarro;Herrera, Jesus A. Sosa;Avila, Jose Pablo Lara;Tobias, Hugo Magdaleno Ramirez;Martinez, Fernando Diaz-Barriga;Ramirez, Rogelio Flores;Vazquez, Angel Gabriel Rodriguez
    • The Plant Pathology Journal
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    • v.34 no.5
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    • pp.381-392
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    • 2018
  • Clavibacter michiganensis subsp. michiganesis (Cmm) is a quarantine-worthy pest in $M{\acute{e}}xico$. The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria. Under controlled conditions a contagion process was induced with Cmm, the disease epidemiology was monitored. Micro-Raman spectroscopy ($532nm\;{\lambda}$ laser) technique was evaluated its performance at assisting on Cmm detection through its characteristic Raman spectrum fingerprint. Our experiment was conducted with tomato plants in a completely randomized block experimental design (13 plants ${\times}$ 4 rows). The Cmm infection was confirmed by 16S rDNA and plants showed symptoms from 48 to 72 h after inoculation, the evolution of the incidence and severity on plant population varied over time and it kept an aggregated spatial pattern. The contagion process reached 79% just 24 days after the epidemic was induced. Micro-Raman spectroscopy proved its speed, efficiency and usefulness as a non-destructive method for the preliminary detection of Cmm. Carotenoid specific bands with wavelengths at 1146 and $1510cm^{-1}$ were the distinguishable markers. Chemometric analyses showed the best performance by the implementation of PCA-LDA supervised classification algorithms applied over Raman spectrum data with 100% of performance in metrics of classifiers (sensitivity, specificity, accuracy, negative and positive predictive value) that allowed us to differentiate Cmm from other endophytic bacteria (Bacillus and Pantoea). The unsupervised KMeans algorithm showed good performance (100, 96, 98, 91 y 100%, respectively).

Generation of Tool Paths for NC Machining of 3D Surfaces by Measurement Data (3차원 측정 곡면의 효율적인 NC 가공을 위한 공구 경로 생성)

  • 구영희
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
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    • 1997.10a
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    • pp.207-212
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    • 1997
  • The purpose of this study is the development of CAM system which can cut and measure any shape by machining center and coordinate measuring machine. The overall goal of the CAM system is to achieve the CNC machining, from digitizing through to final cutting. The hardware of the system comprises PC and machining center, CMM. There are three steps in the CNC machining, (1) workpiece measuring on the CMM, (2) geometric modeling by the CAD system, (3) NC commands generation by the tool path compensated for tool nose radius. It is developed a software package, with which can conduct a micro CAM system in the PC without economical burden.

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Development of a CAM System for Mold Machining using 3D Measurement Data (3차원 측정 데이터를 이용한 금형 가공용 CAM시스템 개발)

  • 구영회
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.7 no.4
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    • pp.79-88
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    • 1998
  • This study deals with the development of CAM system which can machine and measure any shape of mold and die by machining center and coordinate measureing machine . The overall goal of the CAM system is to achieve the mold and die machining , from digitizing through to final cutting. The hardware of the system comprises PC and machining center. CMM. There are three steps in the mold and die machining. (1) measuring of physical model by the CMM, (2) geometric modeling by the CAD system, (3) generation of NC code by the tool path compensated for tool radius. It is developed a software package, with which can conduct a micro CAM system in the PC without economical burden.

Development of computer integrated system for circular error diagnosis of CMM using calibrated ring gauges

  • 박희재;김종후
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 1992.04a
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    • pp.237-241
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    • 1992
  • Error calibration and frequent reverification of coordinate measuring machines(CMMs) and machine tools are currently acknowledge as essential processes in order to maintain high performance of the machines and high quality of products. This paper shows a micro computer integrated circular error calibration system, which is fast and efficient error calibration system

Numerical Analysis of Ventilation System for Dust Removal Inside the Enclosed Conveyor Belt (밀폐된 컨베이어 벨트 내부의 분진 제거를 위한 환기 시스템의 수치해석적 분석)

  • Yeseung Lee;Ki Jin Jeong;Jong Min Kim;Jung-Hun Noh
    • Particle and aerosol research
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    • v.19 no.2
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    • pp.43-53
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    • 2023
  • In this study, the ventilation system of the enclosed conveyor belt for coal transportation was evaluated, and the particle removal efficiency according to the ventilation conditions was identified using computational fluid dynamics and particle behavior analysis. The most effective way to remove dust generated inside the closed conveyor belt is to adjust the position of the exhaust port of the duct so that the air is exhausted around the rear of the conveyor belt. And this method seems to work for another narrow and long spaces where air enters in one direction. In addition, when the air flow rate of the each duct was less than 300 CMM, it was efficient to increase the flow rate of the duct located at the rear of the conveyor belt, and when the flow rate of the each duct was higher than 300 CMM, it was efficient to increase the flow rate of the duct located at the front of the conveyor belt.

Repair of Mold by Cold Spray Deposition and Mechanical Machining (저온 분사 적층과 절삭가공을 이용한 금형보수 사례연구)

  • Kang Hyuk-Jin;Jung Woo-Gyun;Chu Won-Sik;Ahn Sung-Hoon
    • Journal of the Korean Society for Precision Engineering
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    • v.23 no.7 s.184
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    • pp.101-107
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    • 2006
  • Cold gas dynamic spray or cold spray is a novel manufacturing method for coatings. Cold spray is a high rate and direct material deposition process that utilizes the kinetic energy of particles sprayed at high velocity (300-1,200m/s). In this research, a technique to repair the damaged mold by cold spray deposition and mechanical machining was proposed. An aluminum 6061 mold with three-dimensional surface was fabricated, intentionally damaged and material-added by cold spray, and its original geometry was re-obtained successfully by Computer Numerical Control (CNC) machining. To investigate deformation of material caused by cold spray, deposition was conducted on thin aluminum plates ($100mm{\times}100mm{\times}3mm$). The average deformation of the plates was $205{\sim}290{\mu}m$ by Coordinate Measurement Machine (CMM). In addition, the cross section of deposited layer was analyzed by scanning electron microscopy (SEM). To compare variation of hardness, Vickers hardness was measured by micro-hardness tester.

Traveling-wave Ti:LiNbO3 optical modulator capable of complete switching (완전 스위칭이 가능한 Ti:LiNbO3 진행파 광변조기)

  • 곽재곤;김경암;김영문;정은주;피중호;박권동;김창민
    • Korean Journal of Optics and Photonics
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    • v.14 no.5
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    • pp.545-554
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    • 2003
  • Design of the optical modulator composed of a three-waveguide coupler and CPW traveling-wave electrodes was carried out. Switching phenomena of three-waveguide couplers were analyzed by using the coupled mode theory, and the coupling-lengths of the devices were calculated by means of the FDM. CPW traveling-wave electrodes were analysed by the CMM and SOR simulation technique in order to find the conditions of phase-velocity and impedance matching. Traveling-wave modulators were fabricated on z-cut LiNbO$_3$ substrate. Ti was in-diffused in LiNbO$_3$ to make waveguides and Au electrodes were built on the waveguides by the electrolyte technique. The fabricated modulator chip was end-polished, pig-tailed and packaged in a brass mount with K-connector. The insertion loss and the switching voltage of the optical modulator were about 4㏈ and 19V, respectively. Network analyzer was used to obtain the S parameter and the corresponding RF response. From the measurement, parameters of the traveling-wave electrodes were extracted to be Z$_{c}$= 45 Ω, N$_{eff}$=2.20, and $\alpha$$_{0}$=0.055/cm√GHZ. The measured optical response R($\omega$) was compared with the theoretically estimated one, showing both responses agree well. The measurement results revealed that 3㏈ bandwidth turned out to be about 13 GHz.