• 한국식품영양과학회지
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• 제24권6호
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• pp.978-983
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• 1995

The effect of sulfur-containing amino acids on lipid metabolism was studied in rats fed casein as a protein source. Plasma cholesterol, HDL-cholesterol and atherosclerotic index decreased in the cysteine group compared to the methionine group. Plasma triglyceride and phospholipid level were not affected by the supplementation of the sulfur-containing amino acids. The levels of cholesterol and triglyceride in liver decreased by both methionine and cysteine. Cysteine increased the fecal excretion of coprostanol, total neutral steroid and bile acid. The results suggest that plasma cholesterol level is affected by dietary ratio of cysteine/methionine and that the hypocholesterolemic effects of cysteine is, at least in part, through reducing cholesterol absorption from small intestine and through enhancing fecal excretion of bile acids.

• Asian-Australasian Journal of Animal Sciences
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• 제17권12호
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• pp.1725-1728
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• 2004

An experiment was conducted to investigate the efficiency of transfer of dietary iron sources to eggs of laying hens. Eighty ISA-Brown laying birds of 30 wk old were housed in 40 cages of 2 birds each. Eight birds in four cages were assigned to one of the following ten treatments: T1; control, T2; 100 ppm iron supplementation with iron-methionine chelate (Fe-Met-100), T3; Fe-Met- 200, T4; Fe-Met-300, T5; 100 ppm iron supplementation with iron sulfate ($FeSO_4$-100), T6; $FeSO_4$-200, T7; $FeSO_4$-300, T8; 100 ppm iron supplementation with Availa-$Fe^{(R)}$ (Availa-Fe-100), T9; Availa-Fe-200 and T10; Availa-Fe-300. Results of 40 d feeding trial showed that there were no consistent responses in laying performance by source and level of iron supplementation. However, eggshell strength and color were improved by Fe supplementation. Egg iron content was maximized at 10-15 days after feeding supplemental Fe. Fe- Met was the most effective source in enriching Fe of eggs followed by Availa-Fe and $FeSO_4$. Increasing supplementary Fe level more than 100 ppm was not effective in Fe-Met and Availa-Fe treatments. Average Fe enrichment of 18% was achieved after feeding Fe-Met-100 for 15 d. In conclusion, enrichment of Fe in egg could be effectively achieved by supplementation of Fe-Met-100 for 15 d.

• Journal of Animal Science and Technology
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• 제44권4호
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• pp.399-406
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• 2002

Methionine chelate 형태로 Cu 및 Zn의 첨가가 육계의 생산성과 영양소 이용률, 혈청 IgG 수준, 근위의 손상정도(gizzard erosion index) 그리고 간과 배설물의 Cu와 Zn 함량에 미치는 영향을 알아보기 위해 갓 부화한 1,000수의 Ross$^{(R)}$ 병아리를 4처리 5반복, 반복당 50수(암수 각각 25수씩)를 배치하여 5주간 사양시험을 실시하였다. 시험사료는 대조구 사료에 Cu와 Zn를 methionine chelate 형태로 100 ppm씩 단독 또는 혼합첨가하여 만들었다. 증체량은 chelate 첨가구들 모두 대조구 보다 높았고(P<0.05), 사료요구량은 chelate 첨가구들이 대조구 보다 낮았다(P<0.05). Cu와 Zn를 혼합하여 첨가한 처리구의 증체량과 사료효율은 Cu와 Zn를 단독 첨가한 처리구들보다 높은 경향을 나타냈다. 영양소 이용률은 Cu 및 Zn chelate 첨가에 의해 유의한 영향을 받지 않았다. 혈청 IgG 농도는 Cu와 Zn chelate 혼합처리구가 대조구에 비해 유의하게 높았으나(P< 0.05), 근위 궤양 정도는 처리에 따른 차이가 없었다. 간과 배설물 내 Cu 및 Zn 함량은 사료에서 이들 광물질의 함량에 의해 영향을 받았다(P<0.05). 결론적으로 Cu와 Zn chelate 단독 또는 혼합하여 첨가시 육계의 증체량과 사료효율을 개선시키고 혈청 IgG 농도를 증가시키며 간 내 Cu와 Zn 수준은 유의한 영향을 받지 않으나 분 내 Cu와 Zn의 함량은 증가시키는 것으로 나타났다.

• 한국가금학회지
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• 제22권4호
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• pp.213-221
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• 1995

Five experiments were conducted to evaluate the effect of dietary supplemental folic acid(FA) in starting broiler chicks. In the first two experiments, basal diets based on corn and soybean meal contained 0.6 mg/kg FA but no supplemental methionine or choline. At 18 d of age, chicks showed curvilinear responses to folic acid supplementation with maximum growth and feed efficiencies at 1.45 mg/kg FA diet. The liver FA response was also curvilinear but reached a plateau at 1.70 mg/kg FA diet. The basal diet for 3 additional experiments contained soybean meal that had been washed with methanol to remove most of the choline. The diet contained only 0.6 mg /kg folic acid and 754 mg /kg choline. Chicks exhibited a larger growth response to folic acid at low choline levels as evidenced by a significant FA x choline interaction. FA supplementation increased but then decreased valgus leg deformity. Choline supplementation also decreased the incidences of valgus and varus leg deformities and decreased bone ash and increased the incidence of tibial dyschondroplasia. It is concluded that chicks fed diets based on practical ingredients require from 1.45 to 1.70 mg /kg FA diet and also 1.60 mg/kg FA when choline is offered near the NRC recommended level of 1,300 mg/kg.

• Asian-Australasian Journal of Animal Sciences
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• 제10권2호
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• pp.185-191
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• 1997

This experiment was conducted to determine the effects of a low-protein diet supplemented with DL-methionine plus L-cystine (Met + Cys) on the growth performance and fat accumulation of female broiler chicks during the growing period (3-6 wks old). A low-protein diet (17% CP; 3,200 ME kcal/kg) was supplemented with Met + Cys (1.1 : 1.0) at levels 0.75, 0.94, 1.25, 1.31 or 1.50% of diet, respectively. Another diet with 21% CP and 3,200 ME kcal/kg served as the control group. All essential amino acids were adjusted to meet the National Research Council (1984) requirement for chicks. Feed and water were given ad libitum. Body weight of the chicks fed the low-CP diets supplemented with Met + Cys were heavier than those of the control birds. Feed conversion ratio and feed intakes were not significantly different between and among the treatment groups. Similary, abdominal fat content was not significantly different among the various treatments except that of the chicks fed the low CP diet with 1.25% Met + Cys which was higher than that of the control group. Fatty acid synthetase (FAS), acetyl-CoA carboxylase (ACC) activities and carcass protein content were not influenced by dietary treatments. Carcass fat content was lowest in chicks fed low CP diet with 0.75% Met + Cys and highest in the group that received 1.50% Met + Cys supplementation. Liver triglyceride increased as Met + Cys supplementation level increased. Various lipid fraction concentrations (cholesterol ester, free cholesterol, and phospholipid) in the serum went up as Met + Cys increased up to 1.25% after which it levelled off. Results of this experiment suggest that it is possible to reduce dietary protein level from 21% to 17% for growing broiler chicks by the supplementation of Met + Cys when other EAA were sufficient.

• Asian-Australasian Journal of Animal Sciences
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• 제29권3호
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• pp.378-383
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• 2016

The objective of this study was to determine the effects of chromium methionine (Cr-Met) chelate supplementation on blood metabolites and fatty acid profile of beef from Holstein steers during late fattening period. Fifteen Holstein steers were allotted randomly into two groups including the control (non Cr-Met feeding, NCM, ave. body weight [BW] = $483{\pm}25.7kg$) and the treatment (Cr-Met feeding for 4 months, 4CM, ave. $BW=486{\pm}27.5kg$) group. The feeding amount of Cr-Met to animals was limited to 400 ppb/cow/d and was supplemented to total mixed ration. No difference in blood albumin, alkaline phosphatase, urea-nitrogen, calcium, creatine, glucose, total protein, triglyceride, and cholesterol were observed between the treatment groups (p>0.05). The level of high density lipoprotein was higher in the 4CM group than the NCM group, whereas low density lipoprotein was lower in the 4CM group (p<0.05). The fatty acid composition (caprate, laurate, myristate, pentadecanoate, palmitate, palmitoleate, margarate, cis-11 heptadodecanoate, stearate, oleate, trans-vaccenate, linoleate, cis-11 eicosenoate, docosa hexaenoic acid, and docosa pentaenoic acid) of the beef showed no difference between the two groups (p>0.05). The arachidonic acid level tended to be higher in the 4CM than the NCM group (p = 0.07). Cr-Met had no influence (p>0.05) on the ratio of saturated, unsaturated, unsaturated/saturated, monounsaturated/saturated and polyunsaturated/saturated fatty acids whereas the ratio of polyunsaturated fatty acids (PUFA) in the 4CM group was comparatively higher than the NCM group (p<0.05). This study concluded that feeding Cr-Met supplementation in 400 ppb/d to Holstein steers for 4 months during late fattening period can improve some blood metabolites and beef quality by increasing PUFA and gamma-linoleate compositions of beef.

• 한국유기농업학회지
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• 제24권4호
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• pp.957-967
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• 2016

The present study was conducted to investigate effect of dietary protected amino acid on milk yield and composition in dairy cow using meta-analysis. Total 21 research papers were employed in analysis, and mixed model was used for the analysis of effects. Effect of protected methionine (PM) and combination of protected methionine and lysine (PML) were investigated under two different levels of dietary crude protein (CP, <18% and >18%). For performance of dairy cow, milk yield, milk composition including milk fat and protein content and yield and 4% FCM (fat corrected milk) production were used for analysis. In case of milk yield, a trend of increment was found at PM supplementation at low CP (P=0.055). However, the effect of PM at high CP was detected as not significant (P>0.05). In case of milk protein, inclusion of PM at low CP showed significant decrement (P<0.05). However, there was no significant effect of MP on milk protein at high CP (P>0.05). Supplementation of MP at high CP level showed significant increment of milk fat (P<0.05). MP supplementation represented significant increment of 4% FCM production (P<0.05) regardless of dietary CP levels. Effects of PML on milk yield and composition at both of low and high dietary CP were not significant in this study. However, it seem to be that there was a possible positive effect of MPL application at high dietary CP on performance of dairy cow.

• Asian-Australasian Journal of Animal Sciences
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• 제25권2호
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• pp.256-260
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• 2012

The objectives of this study were to investigate the immune responses of broiler chickens fed diets supplemented with different level of chromium methionine (CrMet) in heat stress (HS) condition. Two hundred and eighty eight male broiler chickens (Ross 308) were allocated to four treatment groups (supplementation with 0, 200, 400 or 800 ppb Cr in the form of CrMet) in a completely randomized design. The experiment was conducted at heat stressed condition and all birds were kept under temperature of $33{\pm}2^{\circ}C$. Antibody titers against Newcastle disease virus (NDV) and infectious bronchitis virus (IBV), heterophil to lymphocyte ratios (H/L), and concentration of plasma cortisol (CPC) were measured at 21 and 42 d. At 42 days of age two birds were chosen randomly from each replicate, slaughtered, spleen and bursa of Fabricius were collected, weighed and expressed as a percentage of live body weight. Antibody titers against NDV and IBV at 21 and 42 days of age in broiler fed supplemental CrMet were higher than in broiler chickens fed control diet (p<0.05). CPC level in broiler chickens fed CrMet were significantly (p<0.05) decreased. Increases in lymphocyte counts and consequently a decrease in heterophil to lymphocyte ratios in broiler chickens fed 800 ppb Cr were observed at 21 and 42 d. Supplementation with CrMet had no significant effect on lymphoid organs of broilers. The results suggest that dietary CrMet supplementation at a level of 800 ppb can improve some immune responses of broiler chickens under heat stress conditions.

• Journal of Animal Science and Technology
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• 제47권5호
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• pp.759-768
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• 2005

본 연구는 무기태 크롬($ClCl_3$)와 유기태화 크롬인 Cr-methionine chelate(크라민®)을 첨가하였을 때 in vitro 조건에서 반추위내 발효성상과 반추미생물체 내 Cr과 Methionine을 분석하여 크라민®의 by-pass 여부를 간접적으로 증명하고자 실시하였다. In vitro 소화시험에 이용한 기초영양소는 Jar 당 반추미생물 기초 영양소로 시중에 유통되고 있는 배합사료 7g(DM), 볏짚 2g(DM) 및 Corn silage 2g(DM)을 동일하게 배합하였으며, 시험구로는 대조구(control), $ClCl_3$를 1000ppb 첨가한 T1구 및 Cr 농도가 1000ppb이 되도록 크라민®을 첨가한 T2구를 두었으며, 처리 당 5반복으로 시험을 수행하였다. T2의 pH는 모든 배양시간에서 대조구 및 T1구에 비하여 낮은 경향을 보였으며, 암모니아 농도는 배양 6시간 전까지는 대조구와 T1구에 비하여 T2구가 높은 경향을 보였으나, 배양 6시간 이후에는 모든 처리구가 일정하게 낮게 유지되었다. 총 휘발성지방산 농도는 모든 처리구가 배양시간이 경과함에 다라 지속적으로 증가하였으며, 대조구에 비하여 T1구와 T2구의 농도가 유의적으로 낮았다. In vitro 배양 12시간 동안 미생물체 건물 회수율은 T1구가 가장 낮은 반면에 T2구가 가장 효율적으로 미생물을 증가시켰다. 반추미생물체 내 Cr 농도는 대조구와 T2구간에는 차이가 없었으나(P>0.05), T1구는 유의적(P>0.05)으로 높게 나타났다. 반추미생물체 내 methionine 및 cyctine 농도는 대조구와 T2구간에는 차이(P>0.05)가 없었으나, T1구는 비교적 낮은 경향을 보였다. 본 시험의 결과를 종합해 보면, 크라민®의 첨가에 따른 in vitro 배양액내 pH 및 암모니아 농도를 포함한 발효특성에 대한 부의 영향은 없는 것으로 판단되며, 오히려 미생물체 단백질의 합성에 이용되어 암모니아의 생성량과 총미생물 건물 회수량을 증가시킬 수 있는 것으로 판단된다. 또한 크라민®은 반추 미생물에 의해 상당히 제한적으로 분해되기 때문에 반추위를 회피해서 소장으로 by-pass 되어 이용된 것으로 판단되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권8호
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• pp.1197-1205
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• 2006

This experiment was conducted to compare the effects of feeding DL-2-hydroxy-4-(methylthio)butanoic acid (HMTBA) and DL-methionine (DLM) supplemented corn-soybean-cottonseed meal diets on growth performance, carcass composition, and muscle color of broilers. The trial was designed as a $2{\times}3{\times}2$ factorial experiment, including two methionine (Met) sources (HMTBA and DLM), three equimolar graded levels of Met supplementation (i.e., 0.08, 0.16, and 0.24% in the starter diet and 0.07, 0.14, and 0.21% in the grower and finisher diets, respectively), and two sexes (male and female). Additionally, one basal diet for each sex was formulated to be limiting in Met to test the dosage response of increasing supplemental Met levels. Four hundred and twenty 10-d-old broilers were randomly allotted to 14 treatments (seven each for males and females), with five replicate pens per treatment and six chicks per pen. There was no difference (p>0.05) between the two Met sources in growth performance and muscle deposition of broilers throughout the whole experimental period (d 10 to 49). With the increasing Met supplementation levels, average daily gain was increased (quadratic; p<0.01) during the starter, grower, and overall phases, average daily feed intake was increased (quadratic; p<0.01) during the starter phase, and feed:gain ratio was decreased (quadratic; p<0.05) during the grower and overall phases. At the end of finisher phase, Met supplementation increased breast muscle content (quadratic; p<0.01) and thigh muscle content (linear; p<0.05), and decreased abdominal fat content (quadratic; p<0.02). Compared to the broiler fed DLM, broilers fed HMTBA had superior breast and thigh muscle coloration (p<0.01). Male broilers had higher weight gain and feed intake and better feed conversion than female broilers (p<0.01). The fat content of thigh muscle in female broilers was higher than that of male broilers (p<0.03). The best fit comparison of HMTBA vs. DLM was determined by Schwarz Bayesian Criteria index, which indicated that the average relative bioefficacy of HMTBA vs. DLM was 120% with 95% confidence limit 67 to 172%. These results indicated that Met supplementation improved growth performance and carcass quality of broilers fed corn-soybean-cottonseed meal diets irrespective of Met sources. Compared to DLM, HMTBA has the same molar bioefficacy on improving the growth performance and carcass quality of broilers; however, HMTBA fed birds had superior meat color to DLM fed birds.