• 한국균학회지
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• 제38권2호
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• pp.99-104
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• 2010

2003년부터 2007년까지 국내 여러 지역의 산과 섬 지역을 조사하여 진균에 감염된 죽은 곤충시료를 채집하였다. 채집한 곤충시료에 형성된 진균의 자실체 형태를 조사하고, 채집한 곤충시료에서 총 542균주의 진균을 분리하였다. 분리한 균주의 형태적 및 배양적 특성을 조사하여 동정한 결과, 분리빈도가 많은 곤충기생 진균은 Beauveria속이 3종, Cordyceps속이 8종, Isaria속이 4종이었다. 이외에 분리된 곤충기생 진균은 Metarhizium anisopliae, Nomuraea rileyi, Paecilomyces sp., Verticillium sp.였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제48권1호
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• pp.42-47
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• 2024

The value of silkworms as functional health food materials has increased, as has the interest in its disease control for stable production, and in the economic value of entomopathogenic microorganisms. In this study, we isolated and identified disease-causing fungi from white muscardine silkworms, and confirmed whether this strain could produce white muscardine silkworms. For the analysis of the cause of white muscardine disease in the infected silkworms, the fungi and prokaryotes causing the disease were identified, isolated, and identified using metagenome analysis. Metagenomic analysis detected a large amount of the fungus Metarhizium rileyi in silkworms, and a large amount of the bacterium Enterococcus mundtii, which was presumed to be the causative agent of the disease. For accurate identification of the fungi, these were purified by culture medium, and sequencing and phylogenetic tree analyses were performed using an internal transcribed spacer. As a result, M. rileyi, Cladosporium cladosporioides, and C. tenuissimum were identified. In general, M. rileyi is known to form green conidia, but in this study, white-yellow conidia were formed, indicating that the exact causative agent of the fungal disease cannot be estimated by diagnosing the symptoms. Thus, a diagnostic method is necessary for the continuously collection of required pathogens, and identifying their morphological and genetic characteristics.

• 아시안잔디학회지
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• 제18권4호
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• pp.221-229
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• 2004

경북지방에서 분리한 곤충병원성곰팡이 Beauveria bassiana와 Metarhizium anisopliae를 이용하여 나비목 해충인 거세미나방 (Agrotis segetum), 배추흰나비 (Artogeia rapae), 도둑나방 (Mamestra brassicae), 배추좀나방(Plutella xylostella), 파밤나방 (Spodoptera exigua), 담배거세미나방 (S. litura)의 생물적 방제 가능성을 알아보기 위하여 실험한 결과, 거세미나방 2$\cdot$3령충에 대하여 B. bassiana와 M. anisopliae의 반수치사농도 ($LC_50$)는 접종7일 후에 각각 $4.3\times10^6\;conidia/m\ell$와 $2.4\times10^5\;conidia/m\ell$였다. 배추흰나비 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 접종 7일 후에 각각 $1.2\times10^7\;conidia/m\ell$와 $5.2\times10^6\;conidia/m\ell$였다. 도둑나방 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 접종 7일 후에 각각 $1.5\times10^6\;conidia/m\ell$와 $9.7\times10^5\;conidia/m\ell$였고, 배추좀나방 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 각각 $3.0\times10^6\;conidia/m\ell$와 $1.4\times10^6\;conidia/m\ell$였다. 파밤나방 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 접종 7일 후에 각각 $6.3\times10^6\;conidia/m\ell$와 $2.6\times10^6\;conidia/m\ell$였고, 담배거세미나방 2$\cdot$3령충에 대한 B. bassiana와 M. anisopliae의 $LC_50$는 각각 $1.6\times10^7\;conidia/m\ell$와 $3.4\times10^6\;conidia/m\ell$였다.

• Journal of Microbiology
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• 제35권3호
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• pp.222-227
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• 1997

DNA fragments homologous to chitin synthase gene were amplified from the genomic DNA of Beauveria brongniartii by PCR using degenerate primers. Cloning and sequencing of the PCR-amplified fragments led to the identification of a gene, designated BbCHSl. Comparison of the deduced amino acid sequence of BbCHSl with those of other Euascomycetes revealed that BbCHSl is a gene for class II chitin synthase. The Blastp search of the deduced amino acid sequence of BbCHSl displayed the highest rate of similarity, 95.8%, with CHS2 of Metarhizium unisopliae. Phylogenetic analysis of the amino acid sequences confirmed the taxonomic and evolutionary position of B. brongniartii, which was previously derived by traditional fungal classification based on morphological features.

• 한국균학회지
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• 제49권3호
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• pp.259-283
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• 2021

We isolated nine fungal strains from different environmental materials collected from different locations during a survey of fungal diversity in Korea. Using molecular phylogenetic analyses and morphological characteristics, nine previously undescribed strains were identified and assigned to the species Collariella robusta, Fusicolla acetilerea, Hongkongmyces pedis, Hongkongmyces snookiorum, Mariannaea fusiformis, Metarhizium pemphigi, Pallidocercospora crystallina, Scopulariopsis candida, and Volutella citrinella. Diverse environmental samples may thus be a promising source for isolating and investigating novel fungal species, thus sampling efforts should be increased in future studies. This study also reports identification of some rare fungal species belonging to the genera Hongkongmyces and Pallidocercospora from Korea.

• 한국유기농업학회지
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• 제31권4호
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• pp.427-439
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• 2023

The silverleaf whitefly, Bemisia tabaci, is a major pest distributing worldwide damaging over 900 host plant species, and is highly resistant to chemical pesti- cides. Due to the high pesticide resistance of whitefly, there is a need for alternatives to chemical control. Entomopathogenic fungi are candidates for biological pesticide that can overcome the resistance problem of chemical pesticide. Therefore, in this study, we tested pathogenicity of the entomopathogenic fungi to select high insec- ticidal activity against whitefly. As a result, IPBL-C (Cordyceps fumosorosea) and IPBL-F (Metarhizium pinghaense) isolates showed high insecticidal activity against whitefly. Additionally, as a result of culturing the selected isolates on spent coffee grounds medium, the conidia of IPBL-F produced on coffee grounds medium showed five times higher heat stability after heat treatment at 45℃ for one hour than conidia produced on PDA medium.

• 한국자연치유학회지
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• 제8권2호
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• pp.78-87
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• 2019

목적: 매미에 기생하는 동충하초를 1990 년부터 2016년까지 매년 7월부터 10월까지 주로 한국에서 수집하고 분류하는 것이 목적이었다. 그러나 네팔, 베트남, 일본, 중국, 태국에서 자주 채집을 하였다. 방법: 매미에 기주로 한 동충하초를 산에서 채집하였다. 결과: 9개 속과 10개 종에 속하는 총 1,104개의 표본이 수집하였다. 가장 많은 수의표본은 Ophiocordyceps (563개)이었고, 이어서 Isaria (361개), Polycephalomyces (73개), Cordyceps (70개), Beauveria (25개), Perennicordyceps (8개), Metarhizium (2개) 그리고 Purpureocillium (2개)이었다. Ophiocordyceps종 중. O. longissima는 563개 중에 총 426개의 표본으로 가장 많이 수집되었으며, 120개가 수집된 O. heteropoda와 17개의 표본이 있는 O. sobolifera이었다. 주로한국에서 채집되었지만 C. ishikariensis는 네팔에서만 채집되었다. 새로운 특징은 Isaria cicada-like synnemata가 같은 숙주에서 C. ishikariensis stromata와 함께 자라는 것을 발견했다. 한국에서는 1,104개 중총 691개의 표본이 제주도 한라산에서 발견되었다. 표본을 수집한 한국의 다른 산들은 대룡, 용문, 삼악, 설악, 구절, 두륜, 백암, 칠갑, 청등, 내장, 월출, 지리, 대룡산 등 이었다. 채집한 샘풀 중에서 3개는 미확정되었다. 결론: 총 1,104개의 표본은 9개의 속(genus), 10종류의 종(species)으로 분류되었고, 수집된 691개의 샘풀은 제주도 한라산에서 채집되었다.

• Journal of the Korean Wood Science and Technology
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• 제52권3호
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• pp.262-275
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• 2024

In Indonesia, Coptotermes curvignathus Holmgren and Cryptotermes cyanocephalus Light termite attacks can damage wood and causing losses of 8.7 trillion rupiah per year. Wood treatment and soil barrier are very important to protect structures and their components from termite infestation. The application of synthetic chemicals that pose risks to the environment and human health. The growing movement to replace these chemicals with new termiticides that are safe for the environment in place of persistent organic pollutants. Efficacy performance in entomopathogenic fungi spores such as that produce decanoic acid, hexadecanoic acid, palmitic acid, and octadecadienoic acid are potential compounds that provide for ecofriendly termite control. Entomopathogenic fungal spores from Metarhizium anisopliae, Trichoderma harzianum, and Beauveria bassiana, as the active ingredient were formulated with nanocellulose was added as an inert ingredient to which helped to deliver the active ingredients on controlling the target pest, and enhance the utility ability of the product fungi to control termites. The mortality of these termites successively entomopathogenic fungi was the main cause of death. The higher concentrations being associated with of nanocellulose used affect increased the number of termites mortality. M. anisopliae paired with 60% nanocellulose concentrate was the best percentage for yielded the greatest C. curvignathus and C. cynocephalus termite control. In the field testing, M. anisopliae provided better, it showed the finest result of termite control rather than B. bassiana and T. harzianum. The results of the research indicate that entomopathogenic fungi can be used for Pest Control Management as the subterranean termite and drywood termite control.

• 한국잠사곤충학회지
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• 제38권2호
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• pp.150-153
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• 1996

1차 액체배양액과 2차 pellet 배지로 이루어지는 대량배양체계의 다른 곤충병원성 곰팡이인 Beauveria bassiana, Beauveria brongniartii, Metarhizum anisopliae, Verticillium lecanii 등에 대한 효용성을 조사하기 위하여 단균사 및 분생포자의 성장율을 비교하였다. 그 결과, 액체배양액에서 단균사의 성장율은 접종 후 72시간에 그 수가 최초 접종수에 비해 103-104배까지 증가함을 확인할 수 있었으며, pellet 배지에서 분생자의 수는 접종 후 3주 째에 103배까지 증가됨을 확인할 수 있었다. 본 실험의 결과 진균의 대량배양을 위해 선발된 1차 액체배양액과 2차 pellet 배지는 여러 곤충병원성 진균의 성장에 효율적인 체계임을 확인할 수 있었다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2014년도 추계학술대회 및 정기총회
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• pp.47-47
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• 2014

Dokdo island is located in the northeastern part of Ulleungdo, known as volcanic island. In total, 53 fungal isolates were isolated from Dokdo island soil sample, using dilution plate technique. The isolates were identified on the basis of morphological characteristics and rDNA ITS sequence analysis. Out of them, 41 isolates were identified at the level of species. The dominant fungal species and genera included Fusarium spp., Mucor sp., Clonostachys spp., and Trichoderma sp. The % sequence identity (the number of matches/the complete alignment length) values via NCBI BLAST searching of EML-IF9, EML-MF30-1 and EML-DDSF4 represented 97.19% (485/499) with Clonostachys cf. rosea (GenBank accession no. KC313107), 98.33% (472/480) with Metarhizium guizhouense (GenBank accession no. HM055445), and 100% (350/350) with Mortierella oligospora (GenBank accession no. JX976032), respectively. Three species of C. rosea, M. guizhouense and M. oligospora represented new records of fungi from Dokdo island, Korea. The antimicrobial activities of the fungal strains varied with tested. Two isolates (EML-MFS30-1 and EML-IF9) showed antifungal activity against several fungi including Fusarium oxysporum and Rhizotonia solani. Clonostachys rosea (EML-IF9) showed strong hydrolytic enzyme activity. Our results showed that the antagonistic fungi including Clonostachys rosea will be used as potential biocontrol agents for control of fungal diseases.