• 대한유전성대사질환학회지
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• 제5권1호
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• pp.76-87
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• 2005

Various disorders cause hyperammonemia during childhood. Amongthem are those caused by inherited defects in urea synthesis and related metabolic pathways. These disorders can be grouped into two types: disorders of the enzymes that comprise the urea cycle, and disorders of the transporters or metabolites of theamino acids related to the urea cycle. Principal clinical features of these disorders are caused by elevated levels of blood ammonium. Additional disease-specific symptoms are related to the particular metabolic defect. These specific clinical manifestations are often due to an excess or lack of specific amino acids. Treatment of urea cycle disorders and related metabolic diseases consists of nutritional restriction of proteins, administration of specific amino acids, and use of alternative pathways for discarding excess nitrogen. Although combinations of these treatments are extensively employed, the prognosis of severe cases remains unsatisfactory. Liver transplantation is one alternative for which a better prognosis is reported.

• 한국수정란이식학회:학술대회논문집
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• 한국수정란이식학회 2006년도 The 6th Intermational Symposium on Developmental Biotechnology
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• pp.59-59
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• 2006

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권5호
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• pp.408-417
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• 2005

Increasing numbers of value added chemicals are being produced using microbial fermentation strategies. Computational modeling and simulation of microbial metabolism is rapidly becoming an enabling technology that is driving a new paradigm to accelerate the bioprocess development cycle. In particular, constraint-based modeling and the development of genome-scale models of industrial microbes are finding increasing utility across many phases of the bioprocess development workflow. Herein, we review and discuss the requirements and trends in the industrial application of this technology as we build toward integrated computational/experimental platforms for bioprocess engineering. Specifically we cover the following topics: (1) genome-scale models as genetically and biochemically consistent representations of metabolic networks; (2) the ability of these models to predict, assess, and interpret metabolic physiology and flux states of metabolism; (3) the model-guided integrative analysis of high throughput 'omics' data; (4) the reconciliation and analysis of on- and off-line fermentation data as well as flux tracing data; (5) model-aided strain design strategies and the integration of calculated biotransformation routes; and (6) control and optimization of the fermentation processes. Collectively, constraint-based modeling strategies are impacting the iterative characterization of metabolic flux states throughout the bioprocess development cycle, while also driving metabolic engineering strategies and fermentation optimization.

• 대한유전성대사질환학회지
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• 제5권1호
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• pp.88-93
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• 2005

• Journal of Microbiology and Biotechnology
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• 제19권1호
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• pp.23-36
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• 2009

In the present work, the metabolic network of primary metabolism of the slow-growing myxobacterium Sorangium cellulosum was reconstructed from the annotated genome sequence of the type strain So ce56. During growth on glucose as the carbon source and asparagine as the nitrogen source, So ce56 showed a very low growth rate of $0.23\;d^{-1}$, equivalent to a doubling time of 3 days. Based on a complete stoichiometric and isotopomer model of the central metabolism, $^{13}C$ metabolic flux analysis was carried out for growth with glucose as carbon and asparagine as nitrogen sources. Normalized to the uptake flux for glucose (100%), cells recruited glycolysis (51%) and the pentose phosphate pathway (48%) as major catabolic pathways. The Entner-Doudoroff pathway and glyoxylate shunt were not active. A high flux through the TCA cycle (118%) enabled a strong formation of ATP, but cells revealed a rather low yield for biomass. Inspection of fluxes linked to energy metabolism revealed that S. cellulosum utilized only 10% of the ATP formed for growth, whereas 90% is required for maintenance. This explains the apparent discrepancy between the relatively low biomass yield and the high flux through the energy-delivering TCA cycle. The total flux of NADPH supply (216%) was higher than the demand for anabolism (156%), indicating additional reactions for balancing of NADPH. The cells further exhibited a highly active metabolic cycle, interconverting $C_3$ and $C_4$ metabolites of glycolysis and the TCA cycle. The present work provides the first insight into fluxes of the primary metabolism of myxobacteria, especially for future investigation on the supply of cofactors, building blocks, and energy in myxobacteria, producing natural compounds of biotechnological interest.

• 생명과학회지
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• 제31권8호
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• pp.778-785
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• 2021

오이 씨앗의 발아는 세포의 지방체내 저장지방의 분해 결과인 acyl-CoA의 글라이옥시좀으로 이동 후 베타 산화의 결과물인 acetyl-CoA의 글라이옥실산 회로로의 유입과 지방의 유동으로 촉발된다. Acetyl-CoA는 글라이옥실산 회로의 가동을 위한 탄소원을 제공하며 시트르산과 말산을 생성하며 글라이옥실산 회로의 작동을 유도한다. 지방 저장 종자의 발아에 있어서 글라이옥실산 회로는 필수적 요소이며, 그 결과물인 말산 및 숙신산의 미토콘드리아로의 이동은 TCA 회로의 가동과 옥살초산의 생성 및 세포질로의 유동으로 PEPCK에 의한 당신생을 가능하게 한다. 즉, 저장 지방을 원료로 여러 대사물질의 생산 및 이동과 다중의 대사경로를 통하여 발아 시 사용 가능한 에너지원인 포도당의 형태로 전환이 이루어진다. 이에 동반하여 많은 유전자의 발현 조절이 이루어지고, 세포내 소기관 특히 미소체로 대표되는 글라이옥시좀은 말산 합성효소(malate synthase)와 이소 시트르산 분해효소(isocitrate lyase)로 특화된다. 또 다른 acetyl-CoA의 유동은 carnitine을 매개로 하는 BOU (A BOUT DE SOUFFLE)의 작동이다. 이것은 카니틴의 대사와 관련하여 고등식물의 발달과 대사과정에서의 중요성이 확인된 것으로 사료된다.

• Bulletin of the Korean Chemical Society
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• 제34권1호
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• pp.35-41
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• 2013

Metabolic analysis of CR6 interacting factor 1 (Crif1) deficient mouse embryonic fibroblasts with impaired oxidative phosphorylation has been carried out using LC-MS/MS and GC-MS methods. Metabolic profiles of the Crif1 deficient cells were comprehensively obtained for the first time. Loss of oxidative phosphorylation functions in mitochondria resulted in cancer-like metabolic reprogramming with consumption of majority of glucose carbon from up-regulated glycolysis to produce lactate, suppressed utilization of glucose carbon in the TCA cycle, increased amounts of amino acids. The changes in metabolic profile of the Crif1 deficient cells are most probably a consequence of metabolic reprogramming to meet the needs of energy balance and anabolic precursors in compensation for the loss of major oxidative phosphorylation functions.

• 한국의류학회지
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• 제25권2호
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• pp.339-349
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• 2001

This study was investigated the effects of ambient temperatures and menstrual cycle on Resting Metabolic Rate(RMR), Rectal Temperature(Tre), Skin(forehead, chest, abdomen, forearm, hand, thigh, leg, foot) Temperatures, and subjective thermal sensations in 8 young Korean females(ages 22-25, voluntarily). The Tre and the Skin Temperatures were measured in once every five minute for one hour. RMR was measured three times at 30 minutes intervals by indirect calorimetry. All measurements were gathered during Luteal Phase(LP), Menstruation(M), and Follicular Phase(FP) at two levels of ambient temperatures; low(17~21$^{\circ}C$) and middle(21.1~$25^{\circ}C$). LP were the highest values during FP and M in RMR, Tre, forehead temperature, chest temperature and abdomen temperature, while the leg(leg and foot) and arm(forearm and hand) temperatures were higher during FP rather than during LP at each ambient temperature. The downward curve of Tre in the experiment was larger during FP than LP. The values in subjective thermal sensations were most comfortable during LP than M and FP at each ambient temperature. The LP-FP differences in core and mean skin temperatures, and resting metabolic rate, were more significant at middle ambient temperatures than at low ambient temperatures.

• 생명과학회지
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• 제28권8호
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• pp.985-991
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• 2018

생물시계(Biological clock)는 생명체에서 나타나는 반복되는 자율적인 리듬을 말하며, 단일세포는 물론 다세포 생명체의 기본적인 대사와 이에 따른 표현형과 행동을 직접적으로 조절하고 있다. 이러한 생물시계는 동면 리듬, 수면 리듬, 심장박동 리듬 및 짝짓기 노래 리듬 등 매우 다양하며, 24시간 이상의 주기를 infradian rhythm, 24시간 주기를 circadian rhythm, 24시간 이내의 짧은 주기를 ultradian rhythm으로 구분한다. 효모 Saccharomyces cerevisiae는 최소 5종 이상의 반복되는 자율적인 리듬이 알려져 있으며, 이중 일부는 생체시계로 인식되고 있다. 본 리뷰에서는 Saccharomyces cerevisiae의 glycolytic oscillation (T= 1~30분), cell cycle-dependent oscillation (T= 2~16 시간), ultradian metabolic oscillation (T= 15~50분), yeast colony oscillation (T= 수 시간) 및 circadian oscillation (T= 24시간)에 대한 연구 결과를 제시하고, 특히 ultradian metabolic oscillation의 특징, 집단 동조인자(population synchronizer), 동조인자의 조절 기작 및 효모 생물시계의 대사공학 분야의 이용성을 제시하여 효모를 이용한 동적 대사조절 및 생물시계 연구가 가능함을 제시하였다.

• 수면정신생리
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• 제19권1호
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• pp.5-10
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• 2012

The release of hormones and the metabolism of human body are controlled by the circadian rhythm related to sleep-wake cycle. Growth hormone, prolactin, thyroid stimulating hormone, cortisol, glucose, and insulin-secretion rates fluctuate according to the sleep-wake cycle. In addition, sleep is related to the appetite regulation and carbohydrate and other energy metabolism. Hypocretin (orexin), an excitatory neuropeptide, regulates waking and diet intake, and the poor sleep increases diet intake. The short sleep duration increases one's body mass index and impairs the function of the endocrine and metabolism, causing increases in the risk of glucose intolerance and diabetes. The poor sleep quality and sleep disorders have similar impact on the metabolic function. In short, the sleep loss and the poor quality of sleep have a detrimental effect on the endocrine and energy metabolism. The improvement of sleep quality by the future research and appropriate clinical treatment would contribute to the decrease of the metabolic diseases such as diabetes.